Characterizing the roles of Met31 and Met32 in coordinating Met4-activated transcription in the absence of Met30.

abstract：:The development and progression of cardiac hypertrophy often leads to heart failure and death, and important modulators of hypertrophy include the histone deacetylase proteins (HDACs). Estrogen inhibits cardiac hypertrophy and progression in animal models and humans. We therefore investigated the influence of 17-β-est...

journal_title：Molecular biology of the cell

authors： Pedram A,Razandi M,Narayanan R,Dalton JT,McKinsey TA,Levin ER

更新日期：2013-12-01 00:00:00

abstract：:Extended coiled-coil proteins of the Golgin family play prominent roles in maintaining the structure and function of the Golgi complex. Here we further investigate the Golgin protein Coy1 and document its function in retrograde transport between early Golgi compartments. Cells that lack Coy1 displayed a reduced half-l...

journal_title：Molecular biology of the cell

authors： Anderson NS,Mukherjee I,Bentivoglio CM,Barlowe C

更新日期：2017-08-09 00:00:00

abstract：:Maturation-promoting factor, consisting of cdc2 protein kinase and a regulatory B-type cyclin, is a universal regulator of meiosis and mitosis in eukaryotes. In Xenopus, there are two subtypes of B-type cyclins, designated B1 and B2, both of which are phosphorylated. In this study, we have investigated the biological ...

journal_title：Molecular biology of the cell

authors： Li J,Meyer AN,Donoghue DJ

更新日期：1995-09-01 00:00:00

abstract：:Yeast TGN resident proteins that frequently cycle between the TGN and endosomes are much more slowly transported to the prevacuolar/late endosomal compartment (PVC) than other proteins. However, TGN protein transport to the PVC is accelerated in mutants lacking function of Inp53p. Inp53p contains a SacI polyphosphoino...

journal_title：Molecular biology of the cell

authors： Ha SA,Torabinejad J,DeWald DB,Wenk MR,Lucast L,De Camilli P,Newitt RA,Aebersold R,Nothwehr SF

更新日期：2003-04-01 00:00:00

abstract：:Due to extensive elaboration of the photoreceptor cilium to form the outer segment, axonemal transport (IFT) in photoreceptors is extraordinarily busy, and retinal degeneration is a component of many ciliopathies. Functional loss of heterotrimeric kinesin-2, a major anterograde IFT motor, causes mislocalized opsin, fo...

journal_title：Molecular biology of the cell

authors： Lopes VS,Jimeno D,Khanobdee K,Song X,Chen B,Nusinowitz S,Williams DS

更新日期：2010-12-01 00:00:00

abstract：:The key regulator of entry into mitosis is the serine/threonine kinase p34cdc2. This kinase is regulated both by association with cyclins and by phosphorylation at several sites. Phosphorylation at Tyr 15 and Thr 14 are believed to inhibit the kinase activity of cdc2. In Schizosaccharomyces pombe, the wee1 (and possib...

journal_title：Molecular biology of the cell

authors： Kornbluth S,Sebastian B,Hunter T,Newport J

更新日期：1994-03-01 00:00:00

abstract：:A cell fraction that would today be termed "the nuclear matrix" was first described and patented in 1948 by Russian investigators. In 1974 this fraction was rediscovered and promoted as a fundamental organizing principle of eukaryotic gene expression. Yet, convincing evidence for this functional role of the nuclear ma...

journal_title：Molecular biology of the cell

authors： Pederson T

更新日期：2000-03-01 00:00:00

abstract：:Doing scientific research can be a challenging, but creative and rewarding career. However, the challenge of doing research is orders of magnitude more difficult for scientists lacking access to the necessary resources. Resource-scarce environments are quite common worldwide and are highly contingent on the financial ...

journal_title：Molecular biology of the cell

authors： Karasmanis EP

更新日期：2018-06-01 00:00:00

abstract：:Disassembly of the epithelial apical junctional complex (AJC), composed of the tight junction (TJ) and adherens junction (AJ), is important for normal tissue remodeling and pathogen-induced disruption of epithelial barriers. Using a calcium depletion model in T84 epithelial cells, we previously found that disassembly ...

journal_title：Molecular biology of the cell

authors： Ivanov AI,McCall IC,Parkos CA,Nusrat A

更新日期：2004-06-01 00:00:00

abstract：:Sm-like (Lsm) proteins function in a variety of RNA-processing events. In yeast, the Lsm2-Lsm8 complex binds and stabilizes the spliceosomal U6 snRNA, whereas the Lsm1-Lsm7 complex functions in mRNA decay. Here we report that a third Lsm complex, consisting of Lsm2-Lsm7 proteins, associates with snR5, a box H/ACA snoR...

journal_title：Molecular biology of the cell

authors： Fernandez CF,Pannone BK,Chen X,Fuchs G,Wolin SL

更新日期：2004-06-01 00:00:00

abstract：:Mutations in the Caenorhabditis elegans unc-84 gene cause defects in nuclear migration and anchoring. We show that endogenous UNC-84 protein colocalizes with Ce-lamin at the nuclear envelope and that the envelope localization of UNC-84 requires Ce-lamin. We also show that during mitosis, UNC-84 remains at the nuclear ...

journal_title：Molecular biology of the cell

authors： Lee KK,Starr D,Cohen M,Liu J,Han M,Wilson KL,Gruenbaum Y

更新日期：2002-03-01 00:00:00

abstract：:The dynein light intermediate chain (LIC) is a subunit unique to the cytoplasmic form of dynein, but how it contributes to dynein function is not fully understood. Previous work has established that the LIC homodimer binds directly to the dynein heavy chain and may mediate the attachment of dynein to centrosomes and o...

journal_title：Molecular biology of the cell

authors： Mische S,He Y,Ma L,Li M,Serr M,Hays TS

更新日期：2008-11-01 00:00:00

abstract：:A cell surface proteoglycan, syndecan-1, has been shown to participate in the maintenance of the epithelial cell morphology. A point mutated activated c-Ha-ras gene under the control of the glucocorticoid inducible MMTV-LTR promoter was transfected into the mouse mammary epithelial cell line, NOG-8. The NOG-8 ras cell...

journal_title：Molecular biology of the cell

authors： Kirjavainen J,Leppä S,Hynes NE,Jalkanen M

更新日期：1993-08-01 00:00:00

abstract：:Chemoattractants induce neutrophil polarization through localized polymerization of F-actin at the leading edge. The suppression of rear and lateral protrusions is required for efficient chemotaxis and involves the temporal and spatial segregation of signaling molecules. We have previously shown that the intracellular...

journal_title：Molecular biology of the cell

authors： Nuzzi PA,Senetar MA,Huttenlocher A

更新日期：2007-03-01 00:00:00

abstract：:The membrane-anchored atlastin GTPase couples nucleotide hydrolysis to the catalysis of homotypic membrane fusion to form a branched endoplasmic reticulum network. Trans dimerization between atlastins anchored in opposing membranes, accompanied by a cross-over conformational change, is thought to draw the membranes to...

journal_title：Molecular biology of the cell

authors： Saini SG,Liu C,Zhang P,Lee TH

更新日期：2014-12-01 00:00:00

abstract：:We examined the relative function of the two classes of guanine nucleotide exchange factors (GEFs) for ADP-ribosylation factors that regulate recruitment of coat proteins on the Golgi complex. Complementary overexpression and RNA-based knockdown approaches established that GBF1 regulates COPI recruitment on cis-Golgi ...

journal_title：Molecular biology of the cell

authors： Manolea F,Claude A,Chun J,Rosas J,Melançon P

更新日期：2008-02-01 00:00:00

abstract：:In vertebrate cells, the nucleoporin Nup358/RanBP2 is a major component of the filaments that emanate from the nuclear pore complex into the cytoplasm. Nup358 forms a complex with SUMOylated RanGAP1, the GTPase activating protein for Ran. RanGAP1 plays a pivotal role in the establishment of a RanGTP gradient across th...

journal_title：Molecular biology of the cell

authors： Hutten S,Flotho A,Melchior F,Kehlenbach RH

更新日期：2008-05-01 00:00:00

abstract：:Inactivation of YME1 in yeast causes several distinct phenotypes: an increased rate of DNA escape from mitochondria, temperature-sensitive growth on nonfermentable carbon sources, extremely slow growth when mitochondrial DNA is completely absent from the cell, and altered morphology of the mitochondrial compartment. T...

journal_title：Molecular biology of the cell

authors： Weber ER,Hanekamp T,Thorsness PE

更新日期：1996-02-01 00:00:00

abstract：:Cholesterol is an essential constituent of membranes in mammalian cells. The plasma membrane and the endocytic recycling compartment (ERC) are both highly enriched in cholesterol. The abundance and distribution of cholesterol among organelles are tightly controlled by a combination of mechanisms involving vesicular an...

journal_title：Molecular biology of the cell

authors： Iaea DB,Mao S,Lund FW,Maxfield FR

更新日期：2017-04-15 00:00:00

abstract：:Yeast and vertebrate nuclear pores display significant morphological similarity by electron microscopy, but sequence similarity between the respective proteins has been more difficult to observe. Herein we have identified a vertebrate nucleoporin, Nup93, in both human and Xenopus that has proved to be an evolutionaril...

journal_title：Molecular biology of the cell

authors： Grandi P,Dang T,Pané N,Shevchenko A,Mann M,Forbes D,Hurt E

更新日期：1997-10-01 00:00:00

abstract：:Cells have evolved an autoregulatory mechanism to dampen variations in the concentration of tubulin monomer that is available to polymerize into microtubules (MTs), a process that is known as tubulin autoregulation. However, thermodynamic analysis of MT polymerization predicts that the concentration of free tubulin mo...

journal_title：Molecular biology of the cell

authors： Mooney DJ,Hansen LK,Langer R,Vacanti JP,Ingber DE

更新日期：1994-12-01 00:00:00

abstract：:Although p38 activity is reported to be required as cells enter mitosis for proper spindle assembly and checkpoint function, its role during the division process remains controversial in lieu of direct data. We therefore conducted live cell studies to determine the effect on mitosis of inhibiting or depleting p38. We ...

journal_title：Molecular biology of the cell

authors： Lee K,Kenny AE,Rieder CL

更新日期：2010-07-01 00:00:00

abstract：:The proper execution of premeiotic S phase is essential to both the maintenance of genomic integrity and accurate chromosome segregation during the meiotic divisions. However, the regulation of premeiotic S phase remains poorly defined in metazoa. Here, we identify the p21(Cip1)/p27(Kip1)/p57(Kip2)-like cyclin-depende...

journal_title：Molecular biology of the cell

authors： Narbonne-Reveau K,Lilly M

更新日期：2009-04-01 00:00:00

abstract：:We describe the development and characterization of a system that allows the rapid and specific induction of individual genes in the yeast Saccharomyces cerevisiae without changes in nutrients or temperature. The system is based on the chimeric transcriptional activator Gal4dbd.ER.VP16 (GEV). Upon addition of the horm...

journal_title：Molecular biology of the cell

authors： McIsaac RS,Silverman SJ,McClean MN,Gibney PA,Macinskas J,Hickman MJ,Petti AA,Botstein D

更新日期：2011-11-01 00:00:00

abstract：:In mammalian cells, the inheritance of the Golgi apparatus into the daughter cells during each cycle of cell division is mediated by a disassembly and reassembly process, and this process is precisely controlled by phosphorylation and ubiquitination. VCIP135 (valosin-containing protein p97/p47 complex-interacting prot...

journal_title：Molecular biology of the cell

authors： Zhang X,Wang Y

更新日期：2015-06-15 00:00:00

abstract：:In all eucaryotic cell types analyzed, proliferations of the endoplasmic reticulum (ER) can be induced by increasing the levels of certain integral ER proteins. One of the best characterized of these proteins is HMG-CoA reductase, which catalyzes the rate-limiting step in sterol biosynthesis. We have investigated the ...

journal_title：Molecular biology of the cell

authors： Koning AJ,Roberts CJ,Wright RL

更新日期：1996-05-01 00:00:00

abstract：:Transforming growth factor-β (TGF-β) plays critical roles in numerous physiological and pathological responses. Cholesterol, a major plasma membrane component, can have pronounced effects on signaling responses. Cells continually monitor cholesterol content and activate multilayered transcriptional and translational s...

journal_title：Molecular biology of the cell

authors： Shapira KE,Ehrlich M,Henis YI

更新日期：2018-10-01 00:00:00

abstract：:Actin-based protrusions are important for signaling and migration during development and homeostasis. Defining how different tissues in vivo craft diverse protrusive behaviors using the same genomic toolkit of actin regulators is a current challenge. The actin elongation factors Diaphanous and Enabled both promote bar...

journal_title：Molecular biology of the cell

authors： Nowotarski SH,McKeon N,Moser RJ,Peifer M

更新日期：2014-10-15 00:00:00

abstract：:The [URE3] and [PSI(+)] prions are infectious amyloid forms of Ure2p and Sup35p. Several chaperones influence prion propagation: Hsp104p overproduction destabilizes [PSI(+)], whereas [URE3] is sensitive to excess of Ssa1p or Ydj1p. Here, we show that overproduction of the chaperone, Sse1p, can efficiently cure [URE3]....

journal_title：Molecular biology of the cell

authors： Kryndushkin D,Wickner RB

更新日期：2007-06-01 00:00:00

abstract：:Cdc2 kinase activity is required for triggering entry into mitosis in all known eukaryotes. Elaborate mechanisms have evolved for regulating Cdc2 activity so that mitosis occurs in a timely manner, when preparations for its execution are complete. In Schizosaccharomyces pombe, Wee1 and a related Mik1 kinase are Cdc2-i...

journal_title：Molecular biology of the cell

authors： Campbell SD,Sprenger F,Edgar BA,O'Farrell PH

更新日期：1995-10-01 00:00:00