Mitogen-activated protein kinase phosphatase 2 regulates histone H3 phosphorylation via interaction with vaccinia-related kinase 1.

Abstract:

:Mitogen-activated protein kinase phosphatase 2 (MKP2) is a member of the dual-specificity MKPs that regulate MAP kinase signaling. However, MKP2 functions are still largely unknown. In this study, we showed that MKP2 could regulate histone H3 phosphorylation under oxidative stress conditions. We found that MKP2 inhibited histone H3 phosphorylation by suppressing vaccinia-related kinase 1 (VRK1) activity. Moreover, this regulation was dependent on the selective interaction with VRK1, regardless of its phosphatase activity. The interaction between MKP2 and VRK1 mainly occurred in the chromatin, where histones are abundant. We also observed that the protein level of MKP2 and its interaction with histone H3 increased from G1 to M phase during the cell cycle, which is similar to the VRK1 profile. Furthermore, MKP2 specifically regulated the VRK1-mediated histone H3 phosphorylation at M phase. Taken together, these data suggest a novel function of MKP2 as a negative regulator of VRK1-mediated histone H3 phosphorylation.

journal_name

Mol Biol Cell

authors

Jeong MW,Kang TH,Kim W,Choi YH,Kim KT

doi

10.1091/mbc.E12-06-0456

subject

Has Abstract

pub_date

2013-02-01 00:00:00

pages

373-84

issue

3

eissn

1059-1524

issn

1939-4586

pii

mbc.E12-06-0456

journal_volume

24

pub_type

杂志文章
  • Calreticulin couples calcium release and calcium influx in integrin-mediated calcium signaling.

    abstract::The engagement of integrin alpha7 in E63 skeletal muscle cells by laminin or anti-alpha7 antibodies triggered transient elevations in the intracellular free Ca(2+) concentration that resulted from both inositol triphosphate-evoked Ca(2+) release from intracellular stores and extracellular Ca(2+) influx through voltage...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.11.4.1433

    authors: Kwon MS,Park CS,Choi K,Ahnn J,Kim JI,Eom SH,Kaufman SJ,Song WK

    更新日期:2000-04-01 00:00:00

  • Saccharomyces cerevisiae PTS1 receptor Pex5p interacts with the SH3 domain of the peroxisomal membrane protein Pex13p in an unconventional, non-PXXP-related manner.

    abstract::A number of peroxisome-associated proteins have been described that are involved in the import of proteins into peroxisomes, among which is the receptor for peroxisomal targeting signal 1 (PTS1) proteins Pex5p, the integral membrane protein Pex13p, which contains an Src homology 3 (SH3) domain, and the peripheral memb...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.11.11.3963

    authors: Bottger G,Barnett P,Klein AT,Kragt A,Tabak HF,Distel B

    更新日期:2000-11-01 00:00:00

  • Dual prenylation is required for Rab protein localization and function.

    abstract::The majority of Rab proteins are posttranslationally modified with two geranylgeranyl lipid moieties that enable their stable association with membranes. In this study, we present evidence to demonstrate that there is a specific lipid requirement for Rab protein localization and function. Substitution of different pre...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e02-11-0707

    authors: Calero M,Chen CZ,Zhu W,Winand N,Havas KA,Gilbert PM,Burd CG,Collins RN

    更新日期:2003-05-01 00:00:00

  • Ran1 functions to control the Cdc10/Sct1 complex through Puc1.

    abstract::We have undertaken a biochemical analysis of the regulation of the G1/S-phase transition and commitment to the cell cycle in the fission yeast Schizosaccharomyces pombe. The execution of Start requires the activity of the Cdc2 protein kinase and the Sct1/Cdc10 transcription complex. Progression through G1 also require...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.8.6.1117

    authors: Caligiuri M,Connolly T,Beach D

    更新日期:1997-06-01 00:00:00

  • Vacuole-mediated selective regulation of TORC1-Sch9 signaling following oxidative stress.

    abstract::Target of rapamycin complex 1 (TORC1) is a central cellular signaling coordinator that allows eukaryotic cells to adapt to the environment. In the budding yeast, Saccharomyces cerevisiae, TORC1 senses nitrogen and various stressors and modulates proteosynthesis, nitrogen uptake and metabolism, stress responses, and au...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E17-09-0553

    authors: Takeda E,Jin N,Itakura E,Kira S,Kamada Y,Weisman LS,Noda T,Matsuura A

    更新日期:2018-02-15 00:00:00

  • Cell polarity protein Spa2P associates with proteins involved in actin function in Saccharomyces cerevisiae.

    abstract::Spa2p is a nonessential protein that regulates yeast cell polarity. It localizes early to the presumptive bud site and remains at sites of growth throughout the cell cycle. To understand how Spa2p localization is regulated and to gain insight into its molecular function in cell polarity, we used a coimmunoprecipitatio...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e05-02-0108

    authors: Shih JL,Reck-Peterson SL,Newitt R,Mooseker MS,Aebersold R,Herskowitz I

    更新日期:2005-10-01 00:00:00

  • Dissociation of the Nuf2-Ndc80 complex releases centromeres from the spindle-pole body during meiotic prophase in fission yeast.

    abstract::In the fission yeast Schizosaccharomyces pombe, centromeres remain clustered at the spindle-pole body (SPB) during mitotic interphase. In contrast, during meiotic prophase centromeres dissociate from the SPB. Here we examined the behavior of centromere proteins in living meiotic cells of S. pombe. We show that the Nuf...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e04-11-0996

    authors: Asakawa H,Hayashi A,Haraguchi T,Hiraoka Y

    更新日期:2005-05-01 00:00:00

  • Persistent growth of microtubules at low density.

    abstract::Microtubules (MTs) often form a polarized array with minus-ends anchored at the centrosome and plus-ends extended towards the cell margins. Plus-ends display behavior known as dynamic instability, - transitions between rapid shortening and slow growth. It is known that dynamic instability is regulated locally to ensur...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E20-08-0546

    authors: Burakov A,Vorobjev I,Semenova I,Cowan A,Carson J,Wu Y,Rodionov V

    更新日期:2021-01-13 00:00:00

  • Epidermal growth factor-induced contraction regulates paxillin phosphorylation to temporally separate traction generation from de-adhesion.

    abstract::Directed cell migration is mediated by cycles of protrusion, adhesion, traction generation on the extracellular matrix and retraction. However, how the events after protrusion are timed, and what dictates their temporal order is completely unknown. We used acute epidermal growth factor (EGF) stimulation of epidermal k...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e09-03-0219

    authors: Schneider IC,Hays CK,Waterman CM

    更新日期:2009-07-01 00:00:00

  • An aspartyl cathepsin, CTH3, is essential for proprotein processing during secretory granule maturation in Tetrahymena thermophila.

    abstract::In Tetrahymena thermophila, peptides secreted via dense-core granules, called mucocysts, are generated by proprotein processing. We used expression profiling to identify candidate processing enzymes, which localized as cyan fluorescent protein fusions to mucocysts. Of note, the aspartyl cathepsin Cth3p plays a key rol...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E14-03-0833

    authors: Kumar S,Briguglio JS,Turkewitz AP

    更新日期:2014-08-15 00:00:00

  • Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase RhoA.

    abstract::In mouse embryoid bodies, mutation of the tight junction protein cingulin results in changes in gene expression. Here, we studied the function of cingulin using a gene silencing approach in Madin-Darby canine kidney (MDCK) cells. Cingulin-depleted cells show higher protein and mRNA levels of claudin-2 and ZO-3, increa...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e06-02-0122

    authors: Guillemot L,Citi S

    更新日期:2006-08-01 00:00:00

  • Cdk1-dependent phosphoinhibition of a formin-F-BAR interaction opposes cytokinetic contractile ring formation.

    abstract::In Schizosaccharomyces pombe, cytokinesis requires the assembly and constriction of an actomyosin-based contractile ring (CR). A single essential formin, Cdc12, localizes to the cell middle upon mitotic onset and nucleates the F-actin of the CR. Cdc12 medial recruitment is mediated in part by its direct binding to the...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E17-11-0646

    authors: Willet AH,Bohnert KA,Gould KL

    更新日期:2018-03-15 00:00:00

  • Association of 14-3-3 proteins to beta1-adrenergic receptors modulates Kv11.1 K+ channel activity in recombinant systems.

    abstract::We identify a new mechanism for the beta(1)-adrenergic receptor (beta(1)AR)-mediated regulation of human ether-a-go-go-related gene (HERG) potassium channel (Kv11.1). We find that the previously reported modulatory interaction between Kv11.1 channels and 14-3-3epsilon proteins is competed by wild type beta(1)AR by mea...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e06-05-0422

    authors: Tutor AS,Delpón E,Caballero R,Gómez R,Núñez L,Vaquero M,Tamargo J,Mayor F Jr,Penela P

    更新日期:2006-11-01 00:00:00

  • Positive regulation of Wee1 by Chk1 and 14-3-3 proteins.

    abstract::Wee1 inactivates the Cdc2-cyclin B complex during interphase by phosphorylating Cdc2 on Tyr-15. The activity of Wee1 is highly regulated during the cell cycle. In frog egg extracts, it has been established previously that Xenopus Wee1 (Xwee1) is present in a hypophosphorylated, active form during interphase and underg...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.12.3.551

    authors: Lee J,Kumagai A,Dunphy WG

    更新日期:2001-03-01 00:00:00

  • Binding to the extracellular matrix and proteolytic processing: two key mechanisms regulating vascular endothelial growth factor action.

    abstract::Vascular endothelial growth factor (VEGF, VEGF-A) is a major regulator of physiological and pathological angiogenesis. One feature of VEGF is the existence of multiple isoforms arising from alternative exon splicing. Our initial biochemical and biological studies indicated that such isoforms are uniquely suited to gen...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1091/mbc.e09-07-0590

    authors: Ferrara N

    更新日期:2010-03-01 00:00:00

  • Assembly in G1 phase and long-term stability are unique intrinsic features of CENP-A nucleosomes.

    abstract::Centromeres are the site of kinetochore formation during mitosis. Centromere protein A (CENP-A), the centromere-specific histone H3 variant, is essential for the epigenetic maintenance of centromere position. Previously we showed that newly synthesized CENP-A is targeted to centromeres exclusively during early G1 phas...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E13-01-0034

    authors: Bodor DL,Valente LP,Mata JF,Black BE,Jansen LE

    更新日期:2013-04-01 00:00:00

  • Analysis of prelamin A biogenesis reveals the nucleus to be a CaaX processing compartment.

    abstract::Proteins establish and maintain a distinct intracellular localization by means of targeting, retention, and retrieval signals, ensuring most proteins reside predominantly in one cellular location. The enzymes involved in the maturation of lamin A present a challenge to this paradigm. Lamin A is first synthesized as a ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e08-07-0704

    authors: Barrowman J,Hamblet C,George CM,Michaelis S

    更新日期:2008-12-01 00:00:00

  • Calcineurin regulates cyclin D1 accumulation in growth-stimulated fibroblasts.

    abstract::Calcium (Ca(2+)) and calmodulin (CaM) are required for progression of mammalian cells from quiescence into S phase. In multiple cell types, cyclosporin A causes a G(1) cell cycle arrest, implicating the serine/threonine phosphatase calcineurin as one Ca(2+)/CaM-dependent enzyme required for G(1) transit. Here, we show...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e03-10-0730

    authors: Kahl CR,Means AR

    更新日期:2004-04-01 00:00:00

  • The tomosyn homologue, Sro7, is a direct effector of the Rab GTPase, Sec4, in post-Golgi vesicle tethering.

    abstract::The tomosyn/Sro7 family is thought to play an important role in cell surface trafficking both as an effector of Rab family GTPases and as a regulator of plasma-membrane SNARE function. Recent work has determined the binding site of GTP-bound Sec4 on Sro7. Here we examine the effect of mutations in Sro7 that block Sec4...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E18-02-0138

    authors: Rossi G,Watson K,Kennedy W,Brennwald P

    更新日期:2018-06-15 00:00:00

  • Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi.

    abstract::The late Golgi of the yeast Saccharomyces cerevisiae receives membrane traffic from the secretory pathway as well as retrograde traffic from post-Golgi compartments, but the machinery that regulates these vesicle-docking and fusion events has not been characterized. We have identified three components of a novel prote...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.11.1.305

    authors: Conibear E,Stevens TH

    更新日期:2000-01-01 00:00:00

  • Transition of responsive mechanosensitive elements from focal adhesions to adherens junctions on epithelial differentiation.

    abstract::The skin's epidermis is a multilayered epithelial tissue and the first line of defense against mechanical stress. Its barrier function depends on an integrated assembly and reorganization of cell-matrix and cell-cell junctions in the basal layer and on different intercellular junctions in suprabasal layers. However, h...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E17-06-0387

    authors: Noethel B,Ramms L,Dreissen G,Hoffmann M,Springer R,Rübsam M,Ziegler WH,Niessen CM,Merkel R,Hoffmann B

    更新日期:2018-09-15 00:00:00

  • GLUT4 retention in adipocytes requires two intracellular insulin-regulated transport steps.

    abstract::Insulin regulates glucose uptake into fat and muscle by modulating the distribution of the GLUT4 glucose transporter between the surface and interior of cells. The GLUT4 trafficking pathway overlaps with the general endocytic recycling pathway, but the degree and functional significance of the overlap are not known. I...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e02-02-0071

    authors: Zeigerer A,Lampson MA,Karylowski O,Sabatini DD,Adesnik M,Ren M,McGraw TE

    更新日期:2002-07-01 00:00:00

  • PICH regulates the abundance and localization of SUMOylated proteins on mitotic chromosomes.

    abstract::Proper chromosome segregation is essential for faithful cell division and if not maintained results in defective cell function caused by the abnormal distribution of genetic information. Polo-like kinase 1-interacting checkpoint helicase (PICH) is a DNA translocase essential for chromosome bridge resolution during mit...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E20-03-0180

    authors: Hassebroek VA,Park H,Pandey N,Lerbakken BT,Aksenova V,Arnaoutov A,Dasso M,Azuma Y

    更新日期:2020-11-01 00:00:00

  • A prefoldin-associated WD-repeat protein (WDR92) is required for the correct architectural assembly of motile cilia.

    abstract::WDR92 is a highly conserved WD-repeat protein that has been proposed to be involved in apoptosis and also to be part of a prefoldin-like cochaperone complex. We found that WDR92 has a phylogenetic signature that is generally compatible with it playing a role in the assembly or function of specifically motile cilia. To...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E16-01-0040

    authors: Patel-King RS,King SM

    更新日期:2016-04-15 00:00:00

  • A balance of deubiquitinating enzymes controls cell cycle entry.

    abstract::Protein degradation during the cell cycle is controlled by the opposing activities of ubiquitin ligases and deubiquitinating enzymes (DUBs). Although the functions of ubiquitin ligases in the cell cycle have been studied extensively, the roles of DUBs in this process are less well understood. Here, we used an overexpr...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E18-07-0425

    authors: Mapa CE,Arsenault HE,Conti MM,Poti KE,Benanti JA

    更新日期:2018-11-15 00:00:00

  • Different contributions of nonmuscle myosin IIA and IIB to the organization of stress fiber subtypes in fibroblasts.

    abstract::We demonstrated that myosin IIA and IIB are essential for the formation of transverse arcs and ventral stress fibers, respectively. Furthermore, we illustrated the roles of both isoforms in lamellar flattening and also raised the possibility that actin filaments in ventral stress fibers are in a stretched conformation...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E17-04-0215

    authors: Kuragano M,Uyeda TQP,Kamijo K,Murakami Y,Takahashi M

    更新日期:2018-04-15 00:00:00

  • Fast-acting and nearly gratuitous induction of gene expression and protein depletion in Saccharomyces cerevisiae.

    abstract::We describe the development and characterization of a system that allows the rapid and specific induction of individual genes in the yeast Saccharomyces cerevisiae without changes in nutrients or temperature. The system is based on the chimeric transcriptional activator Gal4dbd.ER.VP16 (GEV). Upon addition of the horm...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E11-05-0466

    authors: McIsaac RS,Silverman SJ,McClean MN,Gibney PA,Macinskas J,Hickman MJ,Petti AA,Botstein D

    更新日期:2011-11-01 00:00:00

  • Regulation of sodium channel activity by capping of actin filaments.

    abstract::Ion transport in various tissues can be regulated by the cortical actin cytoskeleton. Specifically, involvement of actin dynamics in the regulation of nonvoltage-gated sodium channels has been shown. Herein, inside-out patch clamp experiments were performed to study the effect of the heterodimeric actin capping protei...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e02-09-0622

    authors: Shumilina EV,Negulyaev YA,Morachevskaya EA,Hinssen H,Khaitlina SY

    更新日期:2003-04-01 00:00:00

  • Rab GDI: a solubilizing and recycling factor for rab9 protein.

    abstract::Rab proteins are thought to function in the processes by which transport vesicles identify and/or fuse with their respective target membranes. The bulk of these proteins are membrane associated, but a measurable fraction can be found in the cytosol. The cytosolic forms of rab3A, rab11, and Sec4 occur as equimolar comp...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.4.4.425

    authors: Soldati T,Riederer MA,Pfeffer SR

    更新日期:1993-04-01 00:00:00

  • Gfap and Osmr regulation by BRG1 and STAT3 via interchromosomal gene clustering in astrocytes.

    abstract::Long-range chromatin interactions between gene loci in the cell nucleus are important for many biological processes, including transcriptional regulation. Previously, we demonstrated that several genes specifically cluster with the astrocyte-specific gene for glial fibrillary acidic protein (Gfap) during astrocyte dif...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E17-05-0271

    authors: Ito K,Noguchi A,Uosaki Y,Taga T,Arakawa H,Takizawa T

    更新日期:2018-01-15 00:00:00