PKC-mediated phosphorylation of nuclear lamins at a single serine residue regulates interphase nuclear size in Xenopus and mammalian cells.

abstract：:The Rab11 effector Rab11-family interacting protein 2 (Rab11-FIP2) regulates transcytosis through its interactions with Rab11a and myosin Vb. Previous studies implicated Rab11-FIP2 in the establishment of polarity in Madin-Darby canine kidney (MDCK) cells through phosphorylation of Ser-227 by MARK2. Here we examine th...

journal_title：Molecular biology of the cell

authors： Lapierre LA,Avant KM,Caldwell CM,Oztan A,Apodaca G,Knowles BC,Roland JT,Ducharme NA,Goldenring JR

更新日期：2012-06-01 00:00:00

abstract：:Posttranslationally modified forms of tubulin accumulate in the subset of stabilized microtubules (MTs) in cells but are not themselves involved in generating MT stability. We showed previously that stabilized, detyrosinated (Glu) MTs function to localize vimentin intermediate filaments (IFs) in fibroblasts. To determ...

journal_title：Molecular biology of the cell

authors： Kreitzer G,Liao G,Gundersen GG

更新日期：1999-04-01 00:00:00

abstract：:Proteolytic processing of the amyloid-β precursor protein (APP) and generation of amyloid-β peptide (Aβ) are key events in Alzheimer's disease (AD) pathogenesis. Cell biological and genetic evidence has implicated the low-density lipoprotein and sorting receptor LR11/SorLA in AD through mechanisms related to APP and A...

journal_title：Molecular biology of the cell

authors： Herskowitz JH,Offe K,Deshpande A,Kahn RA,Levey AI,Lah JJ

更新日期：2012-07-01 00:00:00

abstract：:Microtubules (MTs) often form a polarized array with minus-ends anchored at the centrosome and plus-ends extended towards the cell margins. Plus-ends display behavior known as dynamic instability, - transitions between rapid shortening and slow growth. It is known that dynamic instability is regulated locally to ensur...

journal_title：Molecular biology of the cell

authors： Burakov A,Vorobjev I,Semenova I,Cowan A,Carson J,Wu Y,Rodionov V

更新日期：2021-01-13 00:00:00

abstract：:In this essay I describe my personal journey from reductionist to systems cell biology and describe how this in turn led to a 3-year sea voyage to explore complex ocean communities. In describing this journey, I hope to convey some important principles that I gleaned along the way. I realized that cellular functions e...

journal_title：Molecular biology of the cell

authors： Karsenti E

更新日期：2012-07-01 00:00:00

abstract：:Microtubules (MTs) in newt mitotic spindles grow faster than MTs in the interphase cytoplasmic microtubule complex (CMTC), yet spindle MTs do not have the long lengths or lifetimes of the CMTC microtubules. Because MTs undergo dynamic instability, it is likely that changes in the durations of growth or shortening are ...

journal_title：Molecular biology of the cell

authors： Gliksman NR,Skibbens RV,Salmon ED

更新日期：1993-10-01 00:00:00

abstract：:Two cDNAs encoding casein kinase-1 have been isolated from a yeast cDNA library and termed CKI1 and CKI2. Each clone encodes a protein of approximately 62,000 Da containing a highly conserved protein kinase domain surrounded by variable amino- and carboxy-terminal domains. The proteins also contain two conserved carbo...

journal_title：Molecular biology of the cell

authors： Wang PC,Vancura A,Mitcheson TG,Kuret J

更新日期：1992-03-01 00:00:00

abstract：:Most membrane proteins are synthesized on and inserted into the membrane of the endoplasmic reticulum (ER), in eukaryote. The widely conserved ER membrane protein complex (EMC) facilitates the biogenesis of a wide range of membrane proteins. In this study, we investigated the EMC function using Drosophila photorecepto...

journal_title：Molecular biology of the cell

authors： Hiramatsu N,Tago T,Satoh T,Satoh AK

更新日期：2019-11-01 00:00:00

abstract：:Pre-rRNA synthesis and processing are key steps in ribosome biogenesis. Although recent evidence in yeast suggests that these two processes are coupled, the nature of their association is unclear. In this report, we analyze the coordination between rDNA transcription and pre-rRNA processing in mammalian cells. We foun...

journal_title：Molecular biology of the cell

authors： Kopp K,Gasiorowski JZ,Chen D,Gilmore R,Norton JT,Wang C,Leary DJ,Chan EK,Dean DA,Huang S

更新日期：2007-02-01 00:00:00

abstract：:The nuclear envelope of metazoans disassembles during mitosis and reforms in late anaphase after sister chromatids have well separated. The coordination of these mitotic events is important for genome stability, yet the temporal control of nuclear envelope reassembly is unknown. Although the steps of nuclear formation...

journal_title：Molecular biology of the cell

authors： Tseng LC,Chen RH

更新日期：2011-09-01 00:00:00

abstract：:Mechanically stressed cells display increased levels of fos message and protein. Although the intracellular signaling pathways responsible for FOS induction have been extensively characterized, we still do not understand the nature of the primary cell mechanotransduction event responsible for converting an externally ...

journal_title：Molecular biology of the cell

authors： Grembowicz KP,Sprague D,McNeil PL

更新日期：1999-04-01 00:00:00

abstract：:Growth factor stimulation induces the formation of dynamic actin structures known as dorsal ruffles. Mammalian actin-binding protein-1 (mAbp1) is an actin-binding protein that has been implicated in regulating clathrin-mediated endocytosis; however, a role for mAbp1 in regulating the dynamics of growth factor-induced ...

journal_title：Molecular biology of the cell

authors： Cortesio CL,Perrin BJ,Bennin DA,Huttenlocher A

更新日期：2010-01-01 00:00:00

abstract：:The receptor-tyrosine kinase (RTK)/Ras/Raf pathway is an essential cascade for mediating growth factor signaling. It is abnormally overactive in almost all human cancers. The downstream targets of the pathway are members of the extracellular regulated kinases (Erk1/2) family, suggesting that this family is a mediator ...

journal_title：Molecular biology of the cell

authors： Smorodinsky-Atias K,Goshen-Lago T,Goldberg-Carp A,Melamed D,Shir A,Mooshayef N,Beenstock J,Karamansha Y,Darlyuk-Saadon I,Livnah O,Ahn NG,Admon A,Engelberg D

更新日期：2016-03-15 00:00:00

abstract：:The Rho family of GTP-binding proteins plays a critical role in a variety of cellular processes, including cytoskeletal reorganization and activation of kinases such as p38 and C-jun N-terminal kinase (JNK) MAPKs. We report here that dominant negative forms of Rac1 and Cdc42Hs inhibit the expression of the muscle-spec...

journal_title：Molecular biology of the cell

authors： Meriane M,Roux P,Primig M,Fort P,Gauthier-Rouvière C

更新日期：2000-08-01 00:00:00

abstract：:Budding yeast treated with hydroxyurea (HU) activate the S phase checkpoint kinase Rad53, which prevents DNA replication forks from undergoing aberrant structural transitions and nuclease processing. Rad53 is also required to prevent premature extension of the mitotic spindle that assembles during a HU-extended S phas...

journal_title：Molecular biology of the cell

authors： Julius J,Peng J,McCulley A,Caridi C,Arnak R,See C,Nugent CI,Feng W,Bachant J

更新日期：2019-10-15 00:00:00

abstract：:Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1) protects cells from oxidative stress via the base excision repair pathway and as a redox transcriptional coactivator. It is required for tumor progression/metastasis, and its up-regulation is associated with cancer resistance. Loss of APE1 expression causes ce...

journal_title：Molecular biology of the cell

authors： Vascotto C,Bisetto E,Li M,Zeef LA,D'Ambrosio C,Domenis R,Comelli M,Delneri D,Scaloni A,Altieri F,Mavelli I,Quadrifoglio F,Kelley MR,Tell G

更新日期：2011-10-01 00:00:00

abstract：:Mechanosensing describes the ability of a cell to sense mechanical cues of its microenvironment, including not only all components of force, stress, and strain but also substrate rigidity, topology, and adhesiveness. This ability is crucial for the cell to respond to the surrounding mechanical cues and adapt to the ch...

journal_title：Molecular biology of the cell

authors： Chen Y,Ju L,Rushdi M,Ge C,Zhu C

更新日期：2017-11-07 00:00:00

abstract：:Chromatin and nuclear pore complexes (NPCs) undergo dramatic changes during mitosis, which in vertebrates and Aspergillus nidulans involves movement of Nup2 from NPCs to the chromatin region to fulfill unknown functions. This transition is shown to require the Cdk1 mitotic kinase and be promoted prematurely by ectopic...

journal_title：Molecular biology of the cell

authors： Markossian S,Suresh S,Osmani AH,Osmani SA

更新日期：2015-02-15 00:00:00

abstract：:TPA-inducible sequence 11b/butyrate response factor 1 (TIS11b/BRF1) belongs to the tristetraprolin (TTP) family of zinc-finger proteins, which bind to mRNAs containing AU-rich elements in their 3'-untranslated region and target them for degradation. Regulation of TTP family function through phosphorylation by p38 MAP ...

journal_title：Molecular biology of the cell

authors： Rataj F,Planel S,Desroches-Castan A,Le Douce J,Lamribet K,Denis J,Feige JJ,Cherradi N

更新日期：2016-12-01 00:00:00

abstract：:The conserved NDR-kinase Dbf2 plays a critical role in cytokinesis in budding yeast. Among its cytokinesis-related substrates is the F-BAR protein Hof1. Hof1 colocalizes at the cell division site with the septin complex and, as mitotic exit progresses, moves to the actomyosin ring (AMR). Neither the function of Hof1 a...

journal_title：Molecular biology of the cell

authors： Meitinger F,Palani S,Hub B,Pereira G

更新日期：2013-05-01 00:00:00

abstract：:Mutations in the Caenorhabditis elegans unc-84 gene cause defects in nuclear migration and anchoring. We show that endogenous UNC-84 protein colocalizes with Ce-lamin at the nuclear envelope and that the envelope localization of UNC-84 requires Ce-lamin. We also show that during mitosis, UNC-84 remains at the nuclear ...

journal_title：Molecular biology of the cell

authors： Lee KK,Starr D,Cohen M,Liu J,Han M,Wilson KL,Gruenbaum Y

更新日期：2002-03-01 00:00:00

abstract：:Myo4p, a single-headed and nonprocessive class V myosin in budding yeast, transports >20 different mRNAs asymmetrically to the bud. Here, we determine the features of the Myo4p motor that are necessary for correct localization of ASH1 mRNA to the daughter cell, a process that also requires the adapter protein She3p an...

journal_title：Molecular biology of the cell

authors： Bookwalter CS,Lord M,Trybus KM

更新日期：2009-07-01 00:00:00

abstract：:E-cadherin plays an essential role in cell polarity and cell-cell adhesion; however, the pathway for delivery of E-cadherin to the basolateral membrane of epithelial cells has not been fully characterized. We first traced the post-Golgi, exocytic transport of GFP-tagged E-cadherin (Ecad-GFP) in unpolarized cells. In l...

journal_title：Molecular biology of the cell

authors： Lock JG,Stow JL

更新日期：2005-04-01 00:00:00

abstract：:Drosophila myosin IB (Myo1B) is one of two class I myosins in the Drosophila genome. In the larval and adult midgut enterocyte, Myo1B is present within the microvillus (MV) of the apical brush border (BB) where it forms lateral tethers between the MV membrane and underlying actin filament core. Expression of green flu...

journal_title：Molecular biology of the cell

authors： Hegan PS,Mermall V,Tilney LG,Mooseker MS

更新日期：2007-11-01 00:00:00

abstract：:We have shown previously that interleukin-4 (IL-4) protects TS1alphabeta cells from apoptosis, but very little is known about the mechanism by which IL-4 exerts this effect. We found that Akt activity, which is dependent on phosphatidylinositol 3 kinase, is reduced in IL-4-deprived TS1alphabeta cells. Overexpression o...

journal_title：Molecular biology of the cell

authors： Cerezo A,Martínez-A C,Lanzarot D,Fischer S,Franke TF,Rebollo A

更新日期：1998-11-01 00:00:00

abstract：:The tail of the yeast myosin V encoded by Myo2p is known to bind several receptors for cargo delivery along polarized actin cables. However, it is not known how Myo2p activity is regulated or how it selects between cargoes. Here we show that Myo2p is reversibly phosphorylated in vivo. A short peptide at the N-terminal...

journal_title：Molecular biology of the cell

authors： Legesse-Miller A,Zhang S,Santiago-Tirado FH,Van Pelt CK,Bretscher A

更新日期：2006-04-01 00:00:00

abstract：:The small G proteins Cdc42, Rac1, and Rac2 regulate the rearrangements of actin and membrane necessary for Fcgamma receptor-mediated phagocytosis by macrophages. Activated, GTP-bound Cdc42, Rac1, and Rac2 bind to the p21-binding domain (PBD) of PAK1, and this interaction provided a basis for microscopic methods to loc...

journal_title：Molecular biology of the cell

authors： Hoppe AD,Swanson JA

更新日期：2004-08-01 00:00:00

abstract：:Tetraploidization, or genome doubling, is a prominent event in tumorigenesis, primarily because cell division in polyploid cells is error-prone and produces aneuploid cells. This study investigates changes in gene expression evoked in acute and adapted tetraploid cells and their effect on cell-cycle progression. Acute...

journal_title：Molecular biology of the cell

authors： Potapova TA,Seidel CW,Box AC,Rancati G,Li R

更新日期：2016-10-15 00:00:00

abstract：:The Golgi apparatus consists of a series of flattened cisternal membranes that are aligned in parallel to form stacks. Cytosolic-oriented Golgi-associated proteins have been identified that may coordinate or maintain the Golgi architecture. Here, we describe a novel GPI-anchored protein, Golgi-resident GPI-anchored pr...

journal_title：Molecular biology of the cell

authors： Li X,Kaloyanova D,van Eijk M,Eerland R,van der Goot G,Oorschot V,Klumperman J,Lottspeich F,Starkuviene V,Wieland FT,Helms JB

更新日期：2007-04-01 00:00:00

abstract：:Gene expression in eukaryotes is an essential process that includes transcription, RNA processing, and export. One important player in this interface is the poly(A)(+)-RNA-binding protein Nab2, which regulates the mRNA poly(A)(+)-tail length and export. Here we show that Nab2 has additional roles during mRNA transcrip...

journal_title：Molecular biology of the cell

authors： González-Aguilera C,Tous C,Babiano R,de la Cruz J,Luna R,Aguilera A

更新日期：2011-08-01 00:00:00