Abstract:
:The nuclear envelope of metazoans disassembles during mitosis and reforms in late anaphase after sister chromatids have well separated. The coordination of these mitotic events is important for genome stability, yet the temporal control of nuclear envelope reassembly is unknown. Although the steps of nuclear formation have been extensively studied in vitro using the reconstitution system from egg extracts, the temporal control can only be studied in vivo. Here, we use time-lapse microscopy to investigate this process in living HeLa cells. We demonstrate that Cdk1 activity prevents premature nuclear envelope assembly and that phosphorylation of the inner nuclear membrane protein lamin B receptor (LBR) by Cdk1 contributes to the temporal control. We further identify a region in the nucleoplasmic domain of LBR that inhibits premature chromatin binding of the protein. We propose that this inhibitory effect is partly mediated by Cdk1 phosphorylation. Furthermore, we show that the reduced chromatin-binding ability of LBR together with Aurora B activity contributes to nuclear envelope breakdown. Our studies reveal for the first time a mechanism that controls the timing of nuclear envelope reassembly through modification of an integral nuclear membrane protein.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Tseng LC,Chen RHdoi
10.1091/mbc.E11-03-0199subject
Has Abstractpub_date
2011-09-01 00:00:00pages
3306-17issue
18eissn
1059-1524issn
1939-4586pii
mbc.E11-03-0199journal_volume
22pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1091/mbc.e04-10-0890
更新日期:2005-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1091/mbc.E15-08-0555
更新日期:2016-07-01 00:00:00
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pub_type: 杂志文章,评审
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