Abstract:
:Three mitochondrial DNA-encoded proteins, Cox1, Cox2, and Cox3, comprise the core of the cytochrome c oxidase complex. Gene-specific translational activators ensure that these respiratory chain subunits are synthesized at the correct location and in stoichiometric ratios to prevent unassembled protein products from generating free oxygen radicals. In the yeast Saccharomyces cerevisiae, the nuclear-encoded proteins Mss51 and Pet309 specifically activate mitochondrial translation of the largest subunit, Cox1. Here we report that Mam33 is a third COX1 translational activator in yeast mitochondria. Mam33 is required for cells to adapt efficiently from fermentation to respiration. In the absence of Mam33, Cox1 translation is impaired, and cells poorly adapt to respiratory conditions because they lack basal fermentative levels of Cox1.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Roloff GA,Henry MFdoi
10.1091/mbc.E15-04-0222subject
Has Abstractpub_date
2015-08-15 00:00:00pages
2885-94issue
16eissn
1059-1524issn
1939-4586pii
mbc.E15-04-0222journal_volume
26pub_type
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