What does it take to get the job done?


:I am extremely honored to be the recipient of the 2015 Women in Cell Biology Junior Award. When I reflect on my journey in science, many great people and memorable experiences come to mind. Some of these encounters were truly career-defining moments. Others provided priceless lessons. In this essay, I recount some of the moments and experiences that influenced my scientific trajectory with the hope that they may inspire others.


Mol Biol Cell


Serpe M




Has Abstract


2015-11-01 00:00:00














  • A conserved mechanism for Bni1- and mDia1-induced actin assembly and dual regulation of Bni1 by Bud6 and profilin.

    abstract::Formins have conserved roles in cell polarity and cytokinesis and directly nucleate actin filament assembly through their FH2 domain. Here, we define the active region of the yeast formin Bni1 FH2 domain and show that it dimerizes. Mutations that disrupt dimerization abolish actin assembly activity, suggesting that di...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Moseley JB,Sagot I,Manning AL,Xu Y,Eck MJ,Pellman D,Goode BL

    更新日期:2004-02-01 00:00:00

  • Tumor necrosis factor-receptor-associated factor-4 is a positive regulator of transforming growth factor-beta signaling that affects neural crest formation.

    abstract::The transforming growth factor (TGF)-beta superfamily regulates cell proliferation, apoptosis, differentiation, migration, and development. Canonical TGFbeta signals are transduced to the nucleus via Smads in both major signaling branches, bone morphogenetic protein (BMP) or Activin/Nodal/TGFbeta. Smurf ubiquitin (Ub)...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Kalkan T,Iwasaki Y,Park CY,Thomsen GH

    更新日期:2009-07-01 00:00:00

  • Coordinated traffic of Grb2 and Ras during epidermal growth factor receptor endocytosis visualized in living cells.

    abstract::Activation of the epidermal growth factor receptor (EGFR) triggers multiple signaling pathways and rapid endocytosis of the epidermal growth factor (EGF)-receptor complexes. To directly visualize the compartmentalization of molecules involved in the major signaling cascade, activation of Ras GTPase, we constructed fus...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Jiang X,Sorkin A

    更新日期:2002-05-01 00:00:00

  • The lipid-anchored ectodomain of influenza virus hemagglutinin (GPI-HA) is capable of inducing nonenlarging fusion pores.

    abstract::GPI-linked hemagglutinin (GPI-HA) of influenza virus was thought to induce hemifusion without pore formation. Cells expressing either HA or GPI-HA were bound to red blood cells, and their fusion was compared by patch-clamp capacitance measurements and fluorescence microscopy. It is now shown that under more optimal fu...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Markosyan RM,Cohen FS,Melikyan GB

    更新日期:2000-04-01 00:00:00

  • NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit.

    abstract::NuMA is an abundant long coiled-coil protein that plays a prominent role in spindle organization during mitosis. In interphase, NuMA is localized to the nucleus and hypothesized to control gene expression and chromatin organization. However, because of the prominent mitotic phenotype upon NuMA loss, its precise functi...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Rajeevan A,Keshri R,Kapoor S,Kotak S

    更新日期:2020-10-15 00:00:00

  • FCH domain only-2 organizes clathrin-coated structures and interacts with Disabled-2 for low-density lipoprotein receptor endocytosis.

    abstract::Clathrin-mediated endocytosis regulates the internalization of many nutrient and signaling receptors. Clathrin and endocytic accessory proteins are recruited to receptors by specific adaptors. The adaptor Disabled-2 (Dab2) recruits its cargoes, including the low-density lipoprotein receptor (LDLR), and mediates endocy...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Mulkearns EE,Cooper JA

    更新日期:2012-04-01 00:00:00

  • Deficiency of the BiP cochaperone ERdj4 causes constitutive endoplasmic reticulum stress and metabolic defects.

    abstract::Endoplasmic reticulum-localized DnaJ 4 (ERdj4) is an immunoglobulin-binding protein (BiP) cochaperone and component of the endoplasmic reticulum-associated degradation (ERAD) pathway that functions to remove unfolded/misfolded substrates from the ER lumen under conditions of ER stress. To elucidate the function of ERd...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Fritz JM,Dong M,Apsley KS,Martin EP,Na CL,Sitaraman S,Weaver TE

    更新日期:2014-02-01 00:00:00

  • Analysis of the roles of phosphatidylinositol-4,5-bisphosphate and individual subunits in assembly, localization, and function of Saccharomyces cerevisiae target of rapamycin complex 2.

    abstract::Eukaryotic cell survival requires maintenance of plasma membrane (PM) homeostasis in response to environmental insults and changes in lipid metabolism. In yeast, a key regulator of PM homeostasis is target of rapamycin (TOR) complex 2 (TORC2), a multiprotein complex containing the evolutionarily conserved TOR protein ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Martinez Marshall MN,Emmerstorfer-Augustin A,Leskoske KL,Zhang LH,Li B,Thorner J

    更新日期:2019-06-01 00:00:00

  • Nutrient stress does not cause retrograde transport of cytoplasmic tRNA to the nucleus in evolutionarily diverse organisms.

    abstract::Intracellular trafficking of tRNA was long thought to be a one-way trip from the site of biogenesis in the nucleus to the translation machinery in the cytoplasm. This view has recently been challenged, however, by the discovery that tRNA can move retrograde from the cytoplasm back to the nucleus in Saccharomyces cerev...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Chafe SC,Pierce JB,Eswara MB,McGuire AT,Mangroo D

    更新日期:2011-04-01 00:00:00

  • The PEN1 syntaxin defines a novel cellular compartment upon fungal attack and is required for the timely assembly of papillae.

    abstract::Attack by the host powdery mildew Erysiphe cichoracearum usually results in successful penetration and rapid proliferation of the fungus on Arabidopsis. By contrast, the nonhost barley powdery mildew Blumeria graminis f. sp. hordei (Bgh) typically fails to penetrate Arabidopsis epidermal cells. In both instances the p...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Assaad FF,Qiu JL,Youngs H,Ehrhardt D,Zimmerli L,Kalde M,Wanner G,Peck SC,Edwards H,Ramonell K,Somerville CR,Thordal-Christensen H

    更新日期:2004-11-01 00:00:00

  • Analysis of the yeast kinome reveals a network of regulated protein localization during filamentous growth.

    abstract::The subcellular distribution of kinases and other signaling proteins is regulated in response to cellular cues; however, the extent of this regulation has not been investigated for any gene set in any organism. Here, we present a systematic analysis of protein kinases in the budding yeast, screening for differential l...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Bharucha N,Ma J,Dobry CJ,Lawson SK,Yang Z,Kumar A

    更新日期:2008-07-01 00:00:00

  • Diverse cytopathologies in mitochondrial disease are caused by AMP-activated protein kinase signaling.

    abstract::The complex cytopathology of mitochondrial diseases is usually attributed to insufficient ATP. AMP-activated protein kinase (AMPK) is a highly sensitive cellular energy sensor that is stimulated by ATP-depleting stresses. By antisense-inhibiting chaperonin 60 expression, we produced mitochondrially diseased strains wi...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Bokko PB,Francione L,Bandala-Sanchez E,Ahmed AU,Annesley SJ,Huang X,Khurana T,Kimmel AR,Fisher PR

    更新日期:2007-05-01 00:00:00

  • Selective inactivation of a Fas-associated death domain protein (FADD)-dependent apoptosis and autophagy pathway in immortal epithelial cells.

    abstract::Although evasion of apoptosis is thought to be required for the development of cancer, it is unclear which cell death pathways are evaded. We previously identified a novel epithelial cell death pathway that works in normal cells but is inactivated in tumor cells, implying that it may be targeted during tumor developme...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Thorburn J,Moore F,Rao A,Barclay WW,Thomas LR,Grant KW,Cramer SD,Thorburn A

    更新日期:2005-03-01 00:00:00

  • Dynactin is required for coordinated bidirectional motility, but not for dynein membrane attachment.

    abstract::Transport of cellular and neuronal vesicles, organelles, and other particles along microtubules requires the molecular motor protein dynein (Mallik and Gross, 2004). Critical to dynein function is dynactin, a multiprotein complex commonly thought to be required for dynein attachment to membrane compartments (Karki and...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Haghnia M,Cavalli V,Shah SB,Schimmelpfeng K,Brusch R,Yang G,Herrera C,Pilling A,Goldstein LS

    更新日期:2007-06-01 00:00:00

  • Signal transduction by the platelet integrin alpha IIb beta 3: induction of calcium oscillations required for protein-tyrosine phosphorylation and ligand-induced spreading of stably transfected cells.

    abstract::We demonstrate an example of signal transduction by an integrin and have begun to define the pathway through which this signaling is achieved. We constructed a stably transfected derivative of 293 cells (ATCC 1573) that expresses the platelet integrin GPIIbIIIa (alpha IIb beta 3). This cell line, clone B, adheres to a...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Pelletier AJ,Bodary SC,Levinson AD

    更新日期:1992-09-01 00:00:00

  • The cAMP pathway regulates mRNA decay through phosphorylation of the RNA-binding protein TIS11b/BRF1.

    abstract::TPA-inducible sequence 11b/butyrate response factor 1 (TIS11b/BRF1) belongs to the tristetraprolin (TTP) family of zinc-finger proteins, which bind to mRNAs containing AU-rich elements in their 3'-untranslated region and target them for degradation. Regulation of TTP family function through phosphorylation by p38 MAP ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Rataj F,Planel S,Desroches-Castan A,Le Douce J,Lamribet K,Denis J,Feige JJ,Cherradi N

    更新日期:2016-12-01 00:00:00

  • srGAP1 regulates lamellipodial dynamics and cell migratory behavior by modulating Rac1 activity.

    abstract::The distinct levels of Rac activity differentially regulate the pattern of intrinsic cell migration. However, it remains unknown how Rac activity is modulated and how the level of Rac activity controls cell migratory behavior. Here we show that Slit-Robo GAP 1 (srGAP1) is a modulator of Rac activity in locomotive cell...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Yamazaki D,Itoh T,Miki H,Takenawa T

    更新日期:2013-11-01 00:00:00

  • The G alpha i homologue gna-1 controls multiple differentiation pathways in Neurospora crassa.

    abstract::Heterotrimeric G proteins are components of principal signaling pathways in eukaryotes. In higher organisms, alpha subunits of G proteins have been divided into four families, Gi, Gs, Gq, and G12. We previously identified a G alpha i homologue gna-1 in the filamentous fungus Neurospora crassa. Now we report that delet...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Ivey FD,Hodge PN,Turner GE,Borkovich KA

    更新日期:1996-08-01 00:00:00

  • Cdc14 inhibition by the spindle assembly checkpoint prevents unscheduled centrosome separation in budding yeast.

    abstract::The spindle assembly checkpoint (SAC) is an evolutionarily conserved surveillance mechanism that delays anaphase onset and mitotic exit in response to the lack of kinetochore attachment. The target of the SAC is the E3 ubiquitin ligase anaphase-promoting complex (APC) bound to its Cdc20 activator. The Cdc20/APC comple...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Chiroli E,Rancati G,Catusi I,Lucchini G,Piatti S

    更新日期:2009-05-01 00:00:00

  • Gradients of phosphatidylserine contribute to plasma membrane charge localization and cell polarity in fission yeast.

    abstract::Surface charges at the inner leaflet of the plasma membrane may contribute to regulate the surface recruitment of key signaling factors. Phosphatidylserine (PS) is an abundant charged lipid that may regulate charge distribution in different cell types. Here we characterize the subcellular distribution and function of ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Haupt A,Minc N

    更新日期:2017-01-01 00:00:00

  • Dynamic association of the PI3P-interacting Mon1-Ccz1 GEF with vacuoles is controlled through its phosphorylation by the type 1 casein kinase Yck3.

    abstract::Maturation of organelles in the endolysosomal pathway requires exchange of the early endosomal GTPase Rab5/Vps21 for the late endosomal Rab7/Ypt7. The Rab exchange depends on the guanine nucleotide exchange factor activity of the Mon1-Ccz1 heterodimer for Ypt7. Here we investigate vacuole binding and recycling of Mon1...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Lawrence G,Brown CC,Flood BA,Karunakaran S,Cabrera M,Nordmann M,Ungermann C,Fratti RA

    更新日期:2014-05-01 00:00:00

  • Functional cdc25C dual-specificity phosphatase is required for S-phase entry in human cells.

    abstract::In view of the common regulatory mechanism that induces transcription of the mitotic phosphatase cdc25C and cyclin A at the beginning of S-phase, we investigated whether cdc25C was required for S-phase transit. Here, we show that in both nontransformed human fibroblasts and HeLa cells, cdc25C protein levels significan...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Turowski P,Franckhauser C,Morris MC,Vaglio P,Fernandez A,Lamb NJ

    更新日期:2003-07-01 00:00:00

  • Phosphatidic acid induces ligand-independent epidermal growth factor receptor endocytic traffic through PDE4 activation.

    abstract::Endocytosis modulates EGFR function by compartmentalizing and attenuating or enhancing its ligand-induced signaling. Here we show that it can also control the cell surface versus intracellular distribution of empty/inactive EGFR. Our previous observation that PKA inhibitors induce EGFR internalization prompted us to t...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Norambuena A,Metz C,Jung JE,Silva A,Otero C,Cancino J,Retamal C,Valenzuela JC,Soza A,González A

    更新日期:2010-08-15 00:00:00

  • A mitotic nuclear envelope tether for Gle1 also impacts nuclear and nucleolar architecture.

    abstract::During Aspergillus nidulans mitosis peripheral nuclear pore complex (NPC) proteins (Nups) disperse from the core NPC structure. Unexpectedly, one predicted peripheral Nup, Gle1, remains at the mitotic NE via an unknown mechanism. Gle1 affinity purification identified MtgA ( M: itotic T: ether for G: le1), which tether...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Chemudupati M,Osmani AH,Osmani SA

    更新日期:2016-09-14 00:00:00

  • Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis.

    abstract::Schizosaccharomyces pombe Rho GTPases regulate actin cytoskeleton organization and cell integrity. We studied the fission yeast gene SPBC4F6.12 based on its ability to suppress the thermosensitivity of cdc42-1625 mutant strain. This gene, named pxl1(+), encodes a protein with three LIM domains that is similar to paxil...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Pinar M,Coll PM,Rincón SA,Pérez P

    更新日期:2008-04-01 00:00:00

  • Novel function of keratins 5 and 14 in proliferation and differentiation of stratified epithelial cells.

    abstract::Keratins are cytoplasmic intermediate filament proteins preferentially expressed by epithelial tissues in a site-specific and differentiation-dependent manner. The complex network of keratin filaments in stratified epithelia is tightly regulated during squamous cell differentiation. Keratin 14 (K14) is expressed in mi...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Alam H,Sehgal L,Kundu ST,Dalal SN,Vaidya MM

    更新日期:2011-11-01 00:00:00

  • Direct inhibition of myosin II effectively blocks glioma invasion in the presence of multiple motogens.

    abstract::Anaplastic gliomas, the most common and malignant of primary brain tumors, frequently contain activating mutations and amplifications in promigratory signal transduction pathways. However, targeting these pathways with individual signal transduction inhibitors does not appreciably reduce tumor invasion, because these ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Ivkovic S,Beadle C,Noticewala S,Massey SC,Swanson KR,Toro LN,Bresnick AR,Canoll P,Rosenfeld SS

    更新日期:2012-02-01 00:00:00

  • Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes.

    abstract::The yeast Golgi membrane protein Rer1p is required for the retrieval of various endoplasmic reticulum (ER) membrane proteins such as Sec12p and Sec71p to the ER. We demonstrate here that the transmembrane domain (TMD) of Sec71p, a type-III membrane protein, contains an ER localization signal, which is required for phy...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Sato K,Sato M,Nakano A

    更新日期:2003-09-01 00:00:00

  • Cholesterol depletion enhances TGF-β Smad signaling by increasing c-Jun expression through a PKR-dependent mechanism.

    abstract::Transforming growth factor-β (TGF-β) plays critical roles in numerous physiological and pathological responses. Cholesterol, a major plasma membrane component, can have pronounced effects on signaling responses. Cells continually monitor cholesterol content and activate multilayered transcriptional and translational s...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Shapira KE,Ehrlich M,Henis YI

    更新日期:2018-10-01 00:00:00

  • Phagocytosis of necrotic cells by macrophages is phosphatidylserine dependent and does not induce inflammatory cytokine production.

    abstract::Apoptotic cells are cleared by phagocytosis during development, homeostasis, and pathology. However, it is still unclear how necrotic cells are removed. We compared the phagocytic uptake by macrophages of variants of L929sA murine fibrosarcoma cells induced to die by tumor necrosis factor-induced necrosis or by Fas-me...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Brouckaert G,Kalai M,Krysko DV,Saelens X,Vercammen D,Ndlovu MN,Haegeman G,D'Herde K,Vandenabeele P

    更新日期:2004-03-01 00:00:00