What does it take to get the job done?


:I am extremely honored to be the recipient of the 2015 Women in Cell Biology Junior Award. When I reflect on my journey in science, many great people and memorable experiences come to mind. Some of these encounters were truly career-defining moments. Others provided priceless lessons. In this essay, I recount some of the moments and experiences that influenced my scientific trajectory with the hope that they may inspire others.


Mol Biol Cell


Serpe M




Has Abstract


2015-11-01 00:00:00














  • Essential role of cyclin-G-associated kinase (Auxilin-2) in developing and mature mice.

    abstract::Hsc70 with its cochaperone, either auxilin or GAK, not only uncoats clathrin-coated vesicles but also acts as a chaperone during clathrin-mediated endocytosis. However, because synaptojanin is also involved in uncoating, it is not clear whether GAK is an essential gene. To answer this question, GAK conditional knockou...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Lee DW,Zhao X,Yim YI,Eisenberg E,Greene LE

    更新日期:2008-07-01 00:00:00

  • GRASP depletion-mediated Golgi destruction decreases cell adhesion and migration via the reduction of α5β1 integrin.

    abstract::The Golgi apparatus is a membrane-bound organelle that serves as the center for trafficking and processing of proteins and lipids. To perform these functions, the Golgi forms a multilayer stacked structure held by GRASP55 and GRASP65 trans-oligomers and perhaps their binding partners. Depletion of GRASP proteins disru...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Ahat E,Xiang Y,Zhang X,Bekier ME 2nd,Wang Y

    更新日期:2019-03-15 00:00:00

  • S-phase checkpoint genes safeguard high-fidelity sister chromatid cohesion.

    abstract::Cohesion establishment and maintenance are carried out by proteins that modify the activity of Cohesin, an essential complex that holds sister chromatids together. Constituents of the replication fork, such as the DNA polymerase alpha-binding protein Ctf4, contribute to cohesion in ways that are poorly understood. To ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Warren CD,Eckley DM,Lee MS,Hanna JS,Hughes A,Peyser B,Jie C,Irizarry R,Spencer FA

    更新日期:2004-04-01 00:00:00

  • The formation of the cAMP/protein kinase A-dependent annexin 2-S100A10 complex with cystic fibrosis conductance regulator protein (CFTR) regulates CFTR channel function.

    abstract::Cystic fibrosis results from mutations in the cystic fibrosis conductance regulator protein (CFTR), a cAMP/protein kinase A (PKA) and ATP-regulated Cl(-) channel. CFTR is increasingly recognized as a component of multiprotein complexes and although several inhibitory proteins to CFTR have been identified, protein comp...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Borthwick LA,McGaw J,Conner G,Taylor CJ,Gerke V,Mehta A,Robson L,Muimo R

    更新日期:2007-09-01 00:00:00

  • Peroxisomal membrane proteins contain common Pex19p-binding sites that are an integral part of their targeting signals.

    abstract::Targeting of peroxisomal membrane proteins (PMPs) is a multistep process that requires not only recognition of PMPs in the cytosol but also their insertion into the peroxisomal membrane. As a consequence, targeting signals of PMPs (mPTS) are rather complex. A candidate protein for the PMP recognition event is Pex19p, ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Rottensteiner H,Kramer A,Lorenzen S,Stein K,Landgraf C,Volkmer-Engert R,Erdmann R

    更新日期:2004-07-01 00:00:00

  • Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells.

    abstract::We report the identification of Cdc7/Dbf4 phosphorylation sites in human MCM2 and the determination of the role of Cdc7/Dbf4 phosphorylation of MCM2 in the initiation of DNA replication. Using immunoblotting, immunofluorescence, and high-speed automated cell-imaging analyses with antibodies specific against MCM2 and C...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Tsuji T,Ficarro SB,Jiang W

    更新日期:2006-10-01 00:00:00

  • Fibroblast growth factor receptor-1 (FGFR1) nuclear dynamics reveal a novel mechanism in transcription control.

    abstract::Nuclear FGFR1 acts as a developmental gene regulator in cooperation with FGF-2, RSK1, and CREB-binding protein (CBP). FRAP analysis revealed three nuclear FGFR1 populations: i) a fast mobile, ii) a slower mobile population reflecting chromatin-bound FGFR1, and iii) an immobile FGFR1 population associated with the nucl...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Dunham-Ems SM,Lee YW,Stachowiak EK,Pudavar H,Claus P,Prasad PN,Stachowiak MK

    更新日期:2009-05-01 00:00:00

  • Accumulation of mutant huntingtin fragments in aggresome-like inclusion bodies as a result of insufficient protein degradation.

    abstract::The huntingtin exon 1 proteins with a polyglutamine repeat in the pathological range (51 or 83 glutamines), but not with a polyglutamine tract in the normal range (20 glutamines), form aggresome-like perinuclear inclusions in human 293 Tet-Off cells. These structures contain aggregated, ubiquitinated huntingtin exon 1...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Waelter S,Boeddrich A,Lurz R,Scherzinger E,Lueder G,Lehrach H,Wanker EE

    更新日期:2001-05-01 00:00:00

  • Budding yeast Dma1 and Dma2 participate in regulation of Swe1 levels and localization.

    abstract::Timely down-regulation of the evolutionarily conserved protein kinase Swe1 plays an important role in cell cycle control, as Swe1 can block nuclear division through inhibitory phosphorylation of the catalytic subunit of cyclin-dependent kinase. In particular, Swe1 degradation is important for budding yeast cell surviv...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Raspelli E,Cassani C,Lucchini G,Fraschini R

    更新日期:2011-07-01 00:00:00

  • PICH regulates the abundance and localization of SUMOylated proteins on mitotic chromosomes.

    abstract::Proper chromosome segregation is essential for faithful cell division and if not maintained results in defective cell function caused by the abnormal distribution of genetic information. Polo-like kinase 1-interacting checkpoint helicase (PICH) is a DNA translocase essential for chromosome bridge resolution during mit...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Hassebroek VA,Park H,Pandey N,Lerbakken BT,Aksenova V,Arnaoutov A,Dasso M,Azuma Y

    更新日期:2020-11-01 00:00:00

  • Microphthalmia-associated transcription factor interactions with 14-3-3 modulate differentiation of committed myeloid precursors.

    abstract::The microphthalmia-associated transcription factor (MITF) is required for terminal osteoclast differentiation and is a target for signaling pathways engaged by colony stimulating factor (CSF)-1 and receptor-activator of nuclear factor-kappaB ligand (RANKL). Work presented here demonstrates that MITF can shuttle from c...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Bronisz A,Sharma SM,Hu R,Godlewski J,Tzivion G,Mansky KC,Ostrowski MC

    更新日期:2006-09-01 00:00:00

  • Translocation channel gating kinetics balances protein translocation efficiency with signal sequence recognition fidelity.

    abstract::The transition between the closed and open conformations of the Sec61 complex permits nascent protein insertion into the translocation channel. A critical event in this structural transition is the opening of the lateral translocon gate that is formed by four transmembrane (TM) spans (TM2, TM3, TM7, and TM8 in Sec61p)...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Trueman SF,Mandon EC,Gilmore R

    更新日期:2011-09-01 00:00:00

  • Specific and nonspecific membrane-binding determinants cooperate in targeting phosphatidylinositol transfer protein beta-isoform to the mammalian trans-Golgi network.

    abstract::Phosphatidylinositol transfer proteins (PITPs) regulate the interface between lipid metabolism and specific steps in membrane trafficking through the secretory pathway in eukaryotes. Herein, we describe the cis-acting information that controls PITPbeta localization in mammalian cells. We demonstrate PITPbeta localizes...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Phillips SE,Ile KE,Boukhelifa M,Huijbregts RP,Bankaitis VA

    更新日期:2006-06-01 00:00:00

  • Mitogen-induced distinct epialleles are phosphorylated at either H3S10 or H3S28, depending on H3K27 acetylation.

    abstract::Stimulation of the MAPK pathway results in mitogen- and stress-activated protein kinase 1/2 (MSK1/2)-catalyzed phosphorylation of histone H3 at serine 10 or 28 and expression of immediate-early (IE) genes. In 10T1/2 mouse fibroblasts, phosphorylation of H3S10 and H3S28 occurs on different H3 molecules and in different...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Khan DH,Healy S,He S,Lichtensztejn D,Klewes L,Sharma KL,Lau V,Mai S,Delcuve GP,Davie JR

    更新日期:2017-03-15 00:00:00

  • Positive regulation of Wee1 by Chk1 and 14-3-3 proteins.

    abstract::Wee1 inactivates the Cdc2-cyclin B complex during interphase by phosphorylating Cdc2 on Tyr-15. The activity of Wee1 is highly regulated during the cell cycle. In frog egg extracts, it has been established previously that Xenopus Wee1 (Xwee1) is present in a hypophosphorylated, active form during interphase and underg...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Lee J,Kumagai A,Dunphy WG

    更新日期:2001-03-01 00:00:00

  • Oxidative folding and assembly with transthyretin are sequential events in the biogenesis of retinol binding protein in the endoplasmic reticulum.

    abstract::Retinol-binding protein (RBP) is secreted out of the cell in its ligand-bound holo-form. The apo-form of RBP is selectively retained within the endoplasmic reticulum (ER) by a mechanism that remains unknown. Using isolated microsomal system, we have recapitulated the biogenesis of RBP involving its oxidative folding a...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Selvaraj SR,Bhatia V,Tatu U

    更新日期:2008-12-01 00:00:00

  • Bud6 directs sequential microtubule interactions with the bud tip and bud neck during spindle morphogenesis in Saccharomyces cerevisiae.

    abstract::In budding yeast, spindle polarity relies on a precise temporal program of cytoplasmic microtubule-cortex interactions throughout spindle assembly. Loss of Clb5-dependent kinase activity under conditions of attenuated Cdc28 function disrupts this program, resulting in diploid-specific lethality. Here we show that pola...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Segal M,Bloom K,Reed SI

    更新日期:2000-11-01 00:00:00

  • Actin cables and the exocyst form two independent morphogenesis pathways in the fission yeast.

    abstract::Cell morphogenesis depends on polarized exocytosis. One widely held model posits that long-range transport and exocyst-dependent tethering of exocytic vesicles at the plasma membrane sequentially drive this process. Here, we describe that disruption of either actin-based long-range transport and microtubules or the ex...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Bendezú FO,Martin SG

    更新日期:2011-01-01 00:00:00

  • Dynamics of GBF1, a Brefeldin A-sensitive Arf1 exchange factor at the Golgi.

    abstract::Trafficking through the Golgi apparatus requires members of the Arf family of GTPases, whose activation is regulated by guanine nucleotide exchange factors (GEFs). Once activated, Arf-GTP recruits effectors such as coat complexes and lipid-modifying enzymes to specific membrane sites, creating a domain competent for c...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Niu TK,Pfeifer AC,Lippincott-Schwartz J,Jackson CL

    更新日期:2005-03-01 00:00:00

  • Regulation of sodium channel activity by capping of actin filaments.

    abstract::Ion transport in various tissues can be regulated by the cortical actin cytoskeleton. Specifically, involvement of actin dynamics in the regulation of nonvoltage-gated sodium channels has been shown. Herein, inside-out patch clamp experiments were performed to study the effect of the heterodimeric actin capping protei...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Shumilina EV,Negulyaev YA,Morachevskaya EA,Hinssen H,Khaitlina SY

    更新日期:2003-04-01 00:00:00

  • ER-golgi traffic is a prerequisite for efficient ER degradation.

    abstract::Protein quality control is an essential function of the endoplasmic reticulum. Misfolded proteins unable to acquire their native conformation are retained in the endoplasmic reticulum, retro-translocated back into the cytosol, and degraded via the ubiquitin-proteasome system. We show that efficient degradation of solu...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Taxis C,Vogel F,Wolf DH

    更新日期:2002-06-01 00:00:00

  • Dual function of the NDR-kinase Dbf2 in the regulation of the F-BAR protein Hof1 during cytokinesis.

    abstract::The conserved NDR-kinase Dbf2 plays a critical role in cytokinesis in budding yeast. Among its cytokinesis-related substrates is the F-BAR protein Hof1. Hof1 colocalizes at the cell division site with the septin complex and, as mitotic exit progresses, moves to the actomyosin ring (AMR). Neither the function of Hof1 a...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Meitinger F,Palani S,Hub B,Pereira G

    更新日期:2013-05-01 00:00:00

  • An actin-based protrusion originating from a podosome-enriched region initiates macrophage fusion.

    abstract::Macrophage fusion resulting in the formation of multinucleated giant cells occurs in a variety of chronic inflammatory diseases, yet the mechanism responsible for initiating this process is unknown. Here, we used live cell imaging to show that actin-based protrusions at the leading edge initiate macrophage fusion. Pha...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Faust JJ,Balabiyev A,Heddleston JM,Podolnikova NP,Baluch DP,Chew TL,Ugarova TP

    更新日期:2019-08-01 00:00:00

  • Developing a set of guidelines for your research field: a practical approach.

    abstract::Since 2008, the autophagy community has periodically published a set of guidelines, currently titled "Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy." The newest version of the guidelines was published in 2016. There are many reasons for establishing a set of guidelines in a given researc...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Klionsky DJ

    更新日期:2016-03-01 00:00:00

  • Essential features of the class V myosin from budding yeast for ASH1 mRNA transport.

    abstract::Myo4p, a single-headed and nonprocessive class V myosin in budding yeast, transports >20 different mRNAs asymmetrically to the bud. Here, we determine the features of the Myo4p motor that are necessary for correct localization of ASH1 mRNA to the daughter cell, a process that also requires the adapter protein She3p an...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Bookwalter CS,Lord M,Trybus KM

    更新日期:2009-07-01 00:00:00

  • Cytoplasmic tails of beta 1, beta 2, and beta 7 integrins differentially regulate LFA-1 function in K562 cells.

    abstract::The beta 2 integrin lymphocyte function-associated antigen 1 (LFA-1) mediates activation-dependent adhesion of lymphocytes. To investigate whether lymphocyte-specific elements are essential for LFA-1 function, we expressed LFA-1 in the erythroleukemic cell line K562, which expresses only the integrin very late antigen...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Lub M,van Vliet SJ,Oomen SP,Pieters RA,Robinson M,Figdor CG,van Kooyk Y

    更新日期:1997-04-01 00:00:00

  • Protein kinase Czeta mediates insulin-induced glucose transport through actin remodeling in L6 muscle cells.

    abstract::Protein kinase C (PKC) zeta has been implicated in insulin-induced glucose uptake in skeletal muscle cell, although the underlying mechanism remains unknown. In this study, we investigated the effect of PKCzeta on actin remodeling and glucose transport in differentiated rat L6 muscle cells expressing myc-tagged glucos...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Liu LZ,Zhao HL,Zuo J,Ho SK,Chan JC,Meng Y,Fang FD,Tong PC

    更新日期:2006-05-01 00:00:00

  • Integrin-mediated adhesion of endothelial cells induces JAK2 and STAT5A activation: role in the control of c-fos gene expression.

    abstract::Integrin-mediated adhesion induces several signaling pathways leading to regulation of gene transcription, control of cell cycle entry and survival from apoptosis. Here we investigate the involvement of the Janus kinase (JAK)/signal transducers and activators of transcription (STAT) pathway in integrin-mediated signal...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Brizzi MF,Defilippi P,Rosso A,Venturino M,Garbarino G,Miyajima A,Silengo L,Tarone G,Pegoraro L

    更新日期:1999-10-01 00:00:00

  • Nab2 functions in the metabolism of RNA driven by polymerases II and III.

    abstract::Gene expression in eukaryotes is an essential process that includes transcription, RNA processing, and export. One important player in this interface is the poly(A)(+)-RNA-binding protein Nab2, which regulates the mRNA poly(A)(+)-tail length and export. Here we show that Nab2 has additional roles during mRNA transcrip...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: González-Aguilera C,Tous C,Babiano R,de la Cruz J,Luna R,Aguilera A

    更新日期:2011-08-01 00:00:00

  • Temporal regulation of cell polarity via the interaction of the Ras GTPase Rsr1 and the scaffold protein Bem1.

    abstract::The Cdc42 guanosine triphosphatase (GTPase) plays a central role in polarity development in species ranging from yeast to humans. In budding yeast, a specific growth site is selected in the G1 phase. Rsr1, a Ras GTPase, interacts with Cdc42 and its associated proteins to promote polarized growth at the proper bud site...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Miller KE,Lo WC,Chou CS,Park HO

    更新日期:2019-09-15 00:00:00