What does it take to get the job done?

abstract：:The RNA-binding protein HuD binds to a regulatory element in the 3' untranslated region (3' UTR) of the GAP-43 mRNA. To investigate the functional significance of this interaction, we generated PC12 cell lines in which HuD levels were controlled by transfection with either antisense (pDuH) or sense (pcHuD) constructs....

journal_title：Molecular biology of the cell

authors： Mobarak CD,Anderson KD,Morin M,Beckel-Mitchener A,Rogers SL,Furneaux H,King P,Perrone-Bizzozero NI

更新日期：2000-09-01 00:00:00

abstract：:The AP-2 adaptor complex plays a key role in cargo recognition and clathrin-coated vesicle formation at the plasma membrane. To investigate the functions of individual binding sites and domains of the AP-2 complex in vivo, we have stably transfected HeLa cells with wild-type and mutant small interfering RNA-resistant ...

journal_title：Molecular biology of the cell

authors： Motley AM,Berg N,Taylor MJ,Sahlender DA,Hirst J,Owen DJ,Robinson MS

更新日期：2006-12-01 00:00:00

abstract：:Drosophila bristles display a precise orientation and curvature. An asymmetric extension of the socket cell overlies the newly emerging bristle rudiment to provide direction for bristle elongation, a process thought to be orchestrated by the nerve dendrite lying between these cells. Scanning electron microscopic analy...

journal_title：Molecular biology of the cell

authors： Tilney LG,Connelly PS,Ruggiero L,Vranich KA,Guild GM,Derosier D

更新日期：2004-12-01 00:00:00

abstract：:Timely down-regulation of the evolutionarily conserved protein kinase Swe1 plays an important role in cell cycle control, as Swe1 can block nuclear division through inhibitory phosphorylation of the catalytic subunit of cyclin-dependent kinase. In particular, Swe1 degradation is important for budding yeast cell surviv...

journal_title：Molecular biology of the cell

authors： Raspelli E,Cassani C,Lucchini G,Fraschini R

更新日期：2011-07-01 00:00:00

abstract：:The stem-loop binding protein (SLBP1) binds the 3' stem-loop of histone pre-mRNA and is required for efficient processing of histone transcripts in the nucleus. We examined the localization of SLBP1 in the germinal vesicle of Xenopus laevis oocytes. In spread preparations of germinal vesicle contents, an anti-SLBP1 an...

journal_title：Molecular biology of the cell

authors： Abbott J,Marzluff WF,Gall JG

更新日期：1999-02-01 00:00:00

abstract：:Accurate chromosome alignment at metaphase facilitates the equal segregation of sister chromatids to each of the nascent daughter cells. Lack of proper metaphase alignment is an indicator of defective chromosome congression and aberrant kinetochore-microtubule attachments which in turn promotes chromosome missegregati...

journal_title：Molecular biology of the cell

authors： Braga LG,Prifti DK,Garand C,Saini PK,Elowe S

更新日期：2020-10-21 00:00:00

abstract：:Vac8p is a vacuolar membrane protein that is required for efficient vacuole inheritance and fusion, cytosol-to-vacuole targeting, and sporulation. By analogy to other armadillo domain proteins, including beta-catenin and importin alpha, we hypothesize that Vac8p docks various factors at the vacuole membrane. Two-hybri...

journal_title：Molecular biology of the cell

authors： Pan X,Roberts P,Chen Y,Kvam E,Shulga N,Huang K,Lemmon S,Goldfarb DS

更新日期：2000-07-01 00:00:00

abstract：:A morphogenesis checkpoint in budding yeast delays nuclear division (and subsequent cell cycle progression) in cells that have failed to make a bud. We show that the ability of this checkpoint to delay nuclear division requires the SWE1 gene, encoding a protein kinase that inhibits the master cell cycle regulatory kin...

journal_title：Molecular biology of the cell

authors： Sia RA,Herald HA,Lew DJ

更新日期：1996-11-01 00:00:00

abstract：:HIF-1alpha is the regulated subunit of the HIF-1 transcription factor, which induces transcription of a number of genes involved in the cellular response to hypoxia. The HIF-1alpha protein is rapidly degraded in cells supplied with adequate oxygen but is stabilized in hypoxic cells. Using polysome profile analysis, we...

journal_title：Molecular biology of the cell

authors： Lang KJ,Kappel A,Goodall GJ

更新日期：2002-05-01 00:00:00

abstract：:Clathrin-mediated endocytosis is a fundamental cellular process conserved from yeast to mammals and is an important endocytic route for the internalization of many specific cargos, including activated growth factor receptors. Here we examined changes in tyrosine phosphorylation, a representative output of growth facto...

journal_title：Molecular biology of the cell

authors： Shen H,Ferguson SM,Dephoure N,Park R,Yang Y,Volpicelli-Daley L,Gygi S,Schlessinger J,De Camilli P

更新日期：2011-02-15 00:00:00

abstract：:Microtubule (MT) organization is essential for many cellular events, including mitosis, migration, and cell polarity. Fidgetin (Fign), an ATP-dependent, MT-severing protein, contributes to the regulation of MT configuration by cutting and trimming MT polymers. Functions of Fign have been indicated in neurite outgrowth...

journal_title：Molecular biology of the cell

authors： Hu Z,Feng J,Bo W,Wu R,Dong Z,Liu Y,Qiang L,Liu M

更新日期：2017-02-15 00:00:00

abstract：:The yeast Golgi membrane protein Rer1p is required for the retrieval of various endoplasmic reticulum (ER) membrane proteins such as Sec12p and Sec71p to the ER. We demonstrate here that the transmembrane domain (TMD) of Sec71p, a type-III membrane protein, contains an ER localization signal, which is required for phy...

journal_title：Molecular biology of the cell

authors： Sato K,Sato M,Nakano A

更新日期：2003-09-01 00:00:00

abstract：:The early Caenorhabditis elegans embryo contains abundant transcripts for two alpha- and two beta-tubulins, raising the question of whether each isoform performs specialized functions or simply contributes to total tubulin levels. Our identification of two recessive, complementing alleles of a beta-tubulin that disrup...

journal_title：Molecular biology of the cell

authors： Wright AJ,Hunter CP

更新日期：2003-11-01 00:00:00

abstract：:Vascular endothelial cells are important in a variety of physiological and pathophysiological processes. The growth and functions of vascular endothelial cells are regulated both by soluble mitogenic and differentiation factors and by interactions with the extracellular matrix; however, relatively little is known abou...

journal_title：Molecular biology of the cell

authors： Short SM,Talbott GA,Juliano RL

更新日期：1998-08-01 00:00:00

abstract：:The orderly progression through the cell division cycle is of paramount importance to all organisms, as improper progression through the cycle could result in defects with grave consequences. Previously, our lab has shown that model eukaryotes such as Saccharomyces cerevisiae, Caenorhabditis elegans, and Danio rerio a...

journal_title：Molecular biology of the cell

authors： Chan K,Goldmark JP,Roth MB

更新日期：2010-07-01 00:00:00

abstract：:The transcription factor Sp1 is ubiquitously expressed in different cells and thereby regulates the expression of genes involved in many cellular processes. This study reveals that Sp1 was phosphorylated during the mitotic stage in three epithelial tumor cell lines and one glioma cell line. By using different kinase i...

journal_title：Molecular biology of the cell

authors： Chuang JY,Wang YT,Yeh SH,Liu YW,Chang WC,Hung JJ

更新日期：2008-03-01 00:00:00

abstract：:Studies on physiological modulation of intercellular communication mediated by protein kinases are often complicated by the fact that cells express multiple gap junction proteins (connexins; Cx). Changes in cell coupling can be masked by simultaneous opposite regulation of the gap junction channel types expressed. We ...

journal_title：Molecular biology of the cell

authors： Kwak BR,Hermans MM,De Jonge HR,Lohmann SM,Jongsma HJ,Chanson M

更新日期：1995-12-01 00:00:00

abstract：:The survival motor neuron (SMN) complex fulfils essential functions in the assembly of snRNPs, which are key components in the splicing of pre-mRNAs. Little is known about the regulation of SMN complex activity by posttranslational modification despite its complicated phosphorylation pattern. Several phosphatases had ...

journal_title：Molecular biology of the cell

authors： Husedzinovic A,Neumann B,Reymann J,Draeger-Meurer S,Chari A,Erfle H,Fischer U,Gruss OJ

更新日期：2015-01-15 00:00:00

abstract：:Cell migration is involved in various physiological and pathogenic events, and the complex underlying molecular mechanisms have not been fully elucidated. The simple eukaryote Dictyostelium discoideum displays chemotactic locomotion in stages of its life cycle. By characterizing a Dictyostelium mutant defective in che...

journal_title：Molecular biology of the cell

authors： Lin WC,Wang LC,Pang TL,Chen MY

更新日期：2015-03-15 00:00:00

abstract：:Gja1(Jrt)/+ mice carry a mutation in one allele of the gap junction protein α1 gene (Gja1), resulting in a G60S connexin 43 (Cx43) mutant protein that is dominant negative for Cx43 protein production of <50% of wild-type (WT) levels and significantly reduced gap junction formation and function in osteoblasts and other...

journal_title：Molecular biology of the cell

authors： Zappitelli T,Chen F,Aubin JE

更新日期：2015-03-01 00:00:00

abstract：:Satellite cells (SCs), the resident adult stem cells of skeletal muscle, are required for tissue repair throughout life. While many signaling pathways are known to control SC self-renewal, less is known about the mechanisms underlying the spatiotemporal control of self-renewal during skeletal muscle repair. Here, we m...

journal_title：Molecular biology of the cell

authors： Moyle LA,Cheng RY,Liu H,Davoudi S,Ferreira SA,Nissar AA,Sun Y,Gentleman E,Simmons CA,Gilbert PM

更新日期：2020-07-21 00:00:00

abstract：:A role of Arp2/3 complex in lamellipodia is well established, whereas its roles in filopodia formation remain obscure. We addressed this question in neuronal cells, in which motility is heavily based on filopodia, and we found that Arp2/3 complex is involved in generation of both lamellipodia and filopodia in growth c...

journal_title：Molecular biology of the cell

authors： Korobova F,Svitkina T

更新日期：2008-04-01 00:00:00

abstract：:Intestinal epithelial cells (IECs) exist in a metabolic state of low oxygen tension termed "physiologic hypoxia." An important factor in maintaining intestinal homeostasis is the transcription factor hypoxia-inducible factor (HIF), which is stabilized under hypoxic conditions and mediates IEC homeostatic responses to ...

journal_title：Molecular biology of the cell

authors： Dowdell AS,Cartwright IM,Goldberg MS,Kostelecky R,Ross T,Welch N,Glover LE,Colgan SP

更新日期：2020-09-15 00:00:00

abstract：:Clathrin-mediated endocytosis regulates the internalization of many nutrient and signaling receptors. Clathrin and endocytic accessory proteins are recruited to receptors by specific adaptors. The adaptor Disabled-2 (Dab2) recruits its cargoes, including the low-density lipoprotein receptor (LDLR), and mediates endocy...

journal_title：Molecular biology of the cell

authors： Mulkearns EE,Cooper JA

更新日期：2012-04-01 00:00:00

abstract：:Exposure of neutrophils to chemoattractant induces cell polarization and migration. These behaviors require the asymmetric activation of distinct signaling pathways and cytoskeletal elements in the protruding pseudopod at the front of cells and the retracting uropod at the rear. An important outstanding question is, h...

journal_title：Molecular biology of the cell

authors： Bodin S,Welch MD

更新日期：2005-12-01 00:00:00

abstract：:Previously, we showed that spliceosomal U6 small nuclear RNA (snRNA) transiently passes through the nucleolus. Herein, we report that all individual snRNAs of the [U4/U6.U5] tri-snRNP localize to nucleoli, demonstrated by fluorescence microscopy of nucleolar preparations after injection of fluorescein-labeled snRNA in...

journal_title：Molecular biology of the cell

authors： Gerbi SA,Lange TS

更新日期：2002-09-01 00:00:00

abstract：:Rab2 immunolocalizes to pre-Golgi intermediates (vesicular-tubular clusters [VTCs]) that are the first site of segregation of anterograde- and retrograde-transported proteins and a major peripheral site for COPI recruitment. Our previous work showed that Rab2 Q65L (equivalent to Ras Q61L) inhibited endoplasmic reticul...

journal_title：Molecular biology of the cell

authors： Tisdale EJ

更新日期：1999-06-01 00:00:00

abstract：:An increase in the level of active, GTP-bound Ras is not necessary for transformation of chicken embryo fibroblasts (CEF) by v-Src. This suggests that other Ras-independent pathways contribute to transformation by v-Src. To address the possibility that activation of phosphatidylinositol-3-kinase (PI3K) and the mammali...

journal_title：Molecular biology of the cell

authors： Penuel E,Martin GS

更新日期：1999-06-01 00:00:00

abstract：:Oncostatin M and cAMP signaling stimulate apical surface-directed membrane trafficking and apical lumen development in hepatocytes, both in a protein kinase A (PKA)-dependent manner. Here, we show that oncostatin M, but not cAMP, promotes the A-kinase anchoring protein (AKAP)-dependent anchoring of the PKA regulatory ...

journal_title：Molecular biology of the cell

authors： Wojtal KA,Hoekstra D,van Ijzendoorn SC

更新日期：2007-07-01 00:00:00

abstract：:Anchorage dependence of cell growth, which is mediated by multiple integrin-regulated signaling pathways, is a key defense against cancer metastasis. Detachment of cells from the extracellular matrix triggers caveolin-1-dependent internalization of lipid raft components, which mediates suppression of Rho GTPases, Erk,...

journal_title：Molecular biology of the cell

authors： Norambuena A,Schwartz MA

更新日期：2011-09-01 00:00:00