The G alpha i homologue gna-1 controls multiple differentiation pathways in Neurospora crassa.

abstract：:Intracellular trafficking of tRNA was long thought to be a one-way trip from the site of biogenesis in the nucleus to the translation machinery in the cytoplasm. This view has recently been challenged, however, by the discovery that tRNA can move retrograde from the cytoplasm back to the nucleus in Saccharomyces cerev...

journal_title：Molecular biology of the cell

authors： Chafe SC,Pierce JB,Eswara MB,McGuire AT,Mangroo D

更新日期：2011-04-01 00:00:00

abstract：:Two different human diseases, X-linked myotubular myopathy and Charcot-Marie-Tooth disease, result from mutant MTM1 or MTMR2 lipid phosphatases. Although events involved in endosomal PI(3)P and PI(3,5)P(2) synthesis are well established and pivotal in receptor signaling and degradation, enzymes involved in phosphoinos...

journal_title：Molecular biology of the cell

authors： Cao C,Backer JM,Laporte J,Bedrick EJ,Wandinger-Ness A

更新日期：2008-08-01 00:00:00

abstract：:Cystic fibrosis results from mutations in the cystic fibrosis conductance regulator protein (CFTR), a cAMP/protein kinase A (PKA) and ATP-regulated Cl(-) channel. CFTR is increasingly recognized as a component of multiprotein complexes and although several inhibitory proteins to CFTR have been identified, protein comp...

journal_title：Molecular biology of the cell

authors： Borthwick LA,McGaw J,Conner G,Taylor CJ,Gerke V,Mehta A,Robson L,Muimo R

更新日期：2007-09-01 00:00:00

abstract：:Condensins are highly conserved proteins that are important for chromosome maintenance in nearly all forms of life. Although many organisms employ two forms of the condensin complex, the condensin genes in Tetrahymena have expanded even further. Here we report a form of condensin that is specifically active during sex...

journal_title：Molecular biology of the cell

authors： Howard-Till R,Tian M,Loidl J

更新日期：2019-05-15 00:00:00

abstract：:Gp78 is an ERAD-associated E3 ubiquitin ligase that induces degradation of the mitofusin mitochondrial fusion proteins and mitochondrial fission. Gp78 is localized throughout the ER; however, the anti-Gp78 3F3A monoclonal antibody (mAb) recognizes Gp78 selectively in mitochondria-associated ER domains. Epitope mapping...

journal_title：Molecular biology of the cell

authors： Li L,Gao G,Shankar J,Joshi B,Foster LJ,Nabi IR

更新日期：2015-11-01 00:00:00

abstract：:DYF-1 is a highly conserved protein essential for ciliogenesis in several model organisms. In Caenorhabditis elegans, DYF-1 serves as an essential activator for an anterograde motor OSM-3 of intraflagellar transport (IFT), the ciliogenesis-required motility that mediates the transport of flagellar precursors and remov...

journal_title：Molecular biology of the cell

authors： Fan ZC,Behal RH,Geimer S,Wang Z,Williamson SM,Zhang H,Cole DG,Qin H

更新日期：2010-08-01 00:00:00

abstract：:Actin nucleation factors function to organize, shape, and move membrane-bound organelles, yet they remain poorly defined in relation to disease. Galloway-Mowat syndrome (GMS) is an inherited disorder characterized by microcephaly and nephrosis resulting from mutations in the WDR73 gene. This core clinical phenotype ap...

journal_title：Molecular biology of the cell

authors： Mathiowetz AJ,Baple E,Russo AJ,Coulter AM,Carrano E,Brown JD,Jinks RN,Crosby AH,Campellone KG

更新日期：2017-09-15 00:00:00

abstract：:In neurons certain mRNA transcripts are transported to synapses through mechanisms that are not fully understood. Here we report that the heterogeneous nuclear ribonucleoprotein CBF-A (CArG Box binding Factor A) facilitates dendritic transport and localization of activity-regulated cytoskeleton-associated protein (Arc...

journal_title：Molecular biology of the cell

authors： Raju CS,Fukuda N,López-Iglesias C,Göritz C,Visa N,Percipalle P

更新日期：2011-06-01 00:00:00

abstract：:B cell antigen receptor (BCR) association with lipid rafts, the actin cytoskeleton, and clathrin-coated pits influences B cell signaling and antigen presentation. Although all three cellular structures have been separately implicated in BCR internalization, the relationship between them has not been clearly defined. I...

journal_title：Molecular biology of the cell

authors： Stoddart A,Jackson AP,Brodsky FM

更新日期：2005-05-01 00:00:00

abstract：:Integrin alpha 3A cytoplasmic tail phosphorylation was mapped to amino acid S1042, as determined by mass spectrometry, and confirmed by mutagenesis. This residue occurs within a "QPSXXE" motif conserved in multiple alpha chains (alpha 3A, alpha 6A, alpha 7A), from multiple species. Phosphorylation of alpha 3A and alph...

journal_title：Molecular biology of the cell

authors： Zhang XA,Bontrager AL,Stipp CS,Kraeft SK,Bazzoni G,Chen LB,Hemler ME

更新日期：2001-02-01 00:00:00

abstract：:The AP-2 adaptor complex plays a key role in cargo recognition and clathrin-coated vesicle formation at the plasma membrane. To investigate the functions of individual binding sites and domains of the AP-2 complex in vivo, we have stably transfected HeLa cells with wild-type and mutant small interfering RNA-resistant ...

journal_title：Molecular biology of the cell

authors： Motley AM,Berg N,Taylor MJ,Sahlender DA,Hirst J,Owen DJ,Robinson MS

更新日期：2006-12-01 00:00:00

abstract：:Clearance of misfolded proteins from the ER is central for maintenance of cellular homeostasis. This process requires coordinated recognition, ER-cytosol translocation, and finally ubiquitination-dependent proteasomal degradation. Here, we identify an ER resident seven-transmembrane protein (JAMP) that links ER chaper...

journal_title：Molecular biology of the cell

authors： Tcherpakov M,Broday L,Delaunay A,Kadoya T,Khurana A,Erdjument-Bromage H,Tempst P,Qiu XB,DeMartino GN,Ronai Z

更新日期：2008-11-01 00:00:00

abstract：:The budding yeast formins Bni1 and Bnr1 control the assembly of actin cables. These formins exhibit distinct patterns of localization and polymerize two different populations of cables: Bni1 in the bud and Bnr1 in the mother cell. We generated a functional Bni1-3GFP that improved the visualization of Bni1 in vivo at e...

journal_title：Molecular biology of the cell

authors： Buttery SM,Yoshida S,Pellman D

更新日期：2007-05-01 00:00:00

abstract：:We provide guidelines for using statistical methods to analyze the types of experiments reported in cellular and molecular biology journals such as Molecular Biology of the Cell. Our aim is to help experimentalists use these methods skillfully, avoid mistakes, and extract the maximum amount of information from their l...

journal_title：Molecular biology of the cell

authors： Pollard DA,Pollard TD,Pollard KS

更新日期：2019-06-01 00:00:00

abstract：:Motor neuron (MN) diseases are progressive disorders resulting from degeneration of neuromuscular junctions (NMJs), which form the connection between MNs and muscle fibers. NMJ-in-a-dish models have been developed to examine human MN-associated dysfunction with disease; however such coculture models have randomly orie...

journal_title：Molecular biology of the cell

authors： Happe CL,Tenerelli KP,Gromova AK,Kolb F,Engler AJ

更新日期：2017-07-07 00:00:00

abstract：:Cdc42-associated tyrosine kinase 1 (ACK1) is a specific down-stream effector of Cdc42, a Rho family small G-protein. Previous studies have shown that ACK1 interacts with clathrin heavy chain and is involved in clathrin-coated vesicle endocytosis. Here we report that ACK1 interacted with epidermal growth factor recepto...

journal_title：Molecular biology of the cell

authors： Shen F,Lin Q,Gu Y,Childress C,Yang W

更新日期：2007-03-01 00:00:00

abstract：:Guanine nucleotide exchange factors (GEFs) are the initiators of signaling by every regulatory GTPase, which in turn act to regulate a wide array of essential cellular processes. To date, each family of GTPases is activated by distinct families of GEFs. Bidirectional membrane trafficking is regulated by ADP-ribosylati...

journal_title：Molecular biology of the cell

authors： Pipaliya SV,Schlacht A,Klinger CM,Kahn RA,Dacks J

更新日期：2019-07-15 00:00:00

abstract：:Calreticulin (CRT) is a high-capacity, low-affinity Ca2+-binding protein located in the lumen of the endoplasmic reticulum (ER) of all eukaryotic cells investigated so far. Its high level of conservation among different species suggests that it serves functions fundamental to cell survival. The role originally propose...

journal_title：Molecular biology of the cell

authors： Fasolato C,Pizzo P,Pozzan T

更新日期：1998-06-01 00:00:00

abstract：:Nuclear FGFR1 acts as a developmental gene regulator in cooperation with FGF-2, RSK1, and CREB-binding protein (CBP). FRAP analysis revealed three nuclear FGFR1 populations: i) a fast mobile, ii) a slower mobile population reflecting chromatin-bound FGFR1, and iii) an immobile FGFR1 population associated with the nucl...

journal_title：Molecular biology of the cell

authors： Dunham-Ems SM,Lee YW,Stachowiak EK,Pudavar H,Claus P,Prasad PN,Stachowiak MK

更新日期：2009-05-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, Cdc24p functions at least in part as a guanine-nucleotide-exchange factor for the Rho-family GTPase Cdc42p. A genetic screen designed to identify possible additional targets of Cdc24p instead identified two previously known genes, MSB1 and CLA4, and one novel gene, designated MSB...

journal_title：Molecular biology of the cell

authors： Bi E,Chiavetta JB,Chen H,Chen GC,Chan CS,Pringle JR

更新日期：2000-02-01 00:00:00

abstract：:The Sec61 translocon mediates the translocation of proteins across the endoplasmic reticulum membrane and the lateral integration of transmembrane segments into the lipid bilayer. The structure of the idle translocon is closed by a lumenal plug domain and a hydrophobic constriction ring. To test the function of the ap...

journal_title：Molecular biology of the cell

authors： Junne T,Kocik L,Spiess M

更新日期：2010-05-15 00:00:00

abstract：:A cell fraction that would today be termed "the nuclear matrix" was first described and patented in 1948 by Russian investigators. In 1974 this fraction was rediscovered and promoted as a fundamental organizing principle of eukaryotic gene expression. Yet, convincing evidence for this functional role of the nuclear ma...

journal_title：Molecular biology of the cell

authors： Pederson T

更新日期：2000-03-01 00:00:00

abstract：:Clathrin-mediated endocytosis is a major pathway for uptake of lipid and protein cargo at the plasma membrane. The lattices of clathrin-coated pits and vesicles are comprised of triskelions, each consisting of three oligomerized heavy chains (HC) bound by a light chain (LC). In addition to binding HC, LC interacts wit...

journal_title：Molecular biology of the cell

authors： Newpher TM,Idrissi FZ,Geli MI,Lemmon SK

更新日期：2006-10-01 00:00:00

abstract：:The PDZ domain-containing protein CAL mediates lysosomal trafficking and degradation of CFTR. Here we demonstrate the involvement of a CAL-binding SNARE protein syntaxin 6 (STX6) in this process. Overexpression of STX6, which colocalizes and coimmunoprecipitates with CAL, dramatically reduces the steady-state level an...

journal_title：Molecular biology of the cell

authors： Cheng J,Cebotaru V,Cebotaru L,Guggino WB

更新日期：2010-04-01 00:00:00

abstract：:Activation of the high-osmolarity glycerol (HOG) pathway for osmoregulation in the yeast Saccharomyces cerevisiae involves interaction of the adaptor Ste50p with the cytoplasmic tail of single-transmembrane protein Opy2p. We have determined the solution structure of the Ste50p-RA (Ras association) domain, and it shows...

journal_title：Molecular biology of the cell

authors： Ekiel I,Sulea T,Jansen G,Kowalik M,Minailiuc O,Cheng J,Harcus D,Cygler M,Whiteway M,Wu C

更新日期：2009-12-01 00:00:00

abstract：:Biochemical data have shown that COPI-coated vesicles are tethered to Golgi membranes by a complex of at least three proteins: p115, giantin, and GM130. p115 binds to giantin on the vesicles and to GM130 on the membrane. We now examine the function of this tethering complex in vivo. Microinjection of an N-terminal pep...

journal_title：Molecular biology of the cell

authors： Seemann J,Jokitalo EJ,Warren G

更新日期：2000-02-01 00:00:00

abstract：:In all orders of life, cell cycle progression in proliferating cells is dependent on cell growth, and the extent of growth required for cell cycle progression is proportional to growth rate. Thus, cells growing rapidly in rich nutrients are substantially larger than slow-growing cells. In budding yeast, a conserved si...

journal_title：Molecular biology of the cell

authors： Alcaide-Gavilán M,Lucena R,Banuelos S,Kellogg DR

更新日期：2020-08-15 00:00:00

abstract：:The intermembrane space of mitochondria accommodates the essential mitochondrial intermembrane space assembly (MIA) machinery that catalyzes oxidative folding of proteins. The disulfide bond formation pathway is based on a relay of reactions involving disulfide transfer from the sulfhydryl oxidase Erv1 to Mia40 and fr...

journal_title：Molecular biology of the cell

authors： Böttinger L,Gornicka A,Czerwik T,Bragoszewski P,Loniewska-Lwowska A,Schulze-Specking A,Truscott KN,Guiard B,Milenkovic D,Chacinska A

更新日期：2012-10-01 00:00:00

abstract：:We have isolated mutants in the fission yeast Schizosaccharomyces pombe that are defective in protein glycosylation. A collection of osmotically sensitive mutants was prepared and screened for glycosylation defects using lectin staining as an assay. Mutants singly defective in four glycoprotein synthesis genes (gps1-4...

journal_title：Molecular biology of the cell

authors： Huang KM,Snider MD

更新日期：1995-05-01 00:00:00

abstract：:ARVCF, an armadillo-repeat protein of the p120(ctn) family, associates with classical cadherins and is present in adherens junctions, but its function is poorly understood. Here, we show that ARVCF interacts via a C-terminal PDZ-binding motif with zonula occludens (ZO)-1 and ZO-2. ARVCF and ZO-1 partially colocalize i...

journal_title：Molecular biology of the cell

authors： Kausalya PJ,Phua DC,Hunziker W

更新日期：2004-12-01 00:00:00