Conserved Ark1-related kinases function in a TORC2 signaling network.

abstract：:Glycoprotein gp130, found on the plasma membrane of Dictyostelium discoideum amoebae, was postulated previously to play a role in phagocytosis. The gene for gp130 was cloned and when translated, yielded a 768 amino acid preproprotein of 85.3 kDa. It had nearly 40% similarity to the 138 kDa family of glycoproteins impl...

journal_title：Molecular biology of the cell

authors： Chia CP,Gomathinayagam S,Schmaltz RJ,Smoyer LK

更新日期：2005-06-01 00:00:00

abstract：:The RNA-binding protein HuD binds to a regulatory element in the 3' untranslated region (3' UTR) of the GAP-43 mRNA. To investigate the functional significance of this interaction, we generated PC12 cell lines in which HuD levels were controlled by transfection with either antisense (pDuH) or sense (pcHuD) constructs....

journal_title：Molecular biology of the cell

authors： Mobarak CD,Anderson KD,Morin M,Beckel-Mitchener A,Rogers SL,Furneaux H,King P,Perrone-Bizzozero NI

更新日期：2000-09-01 00:00:00

abstract：:The crystalline lens is an attractive system to study the biology of intercellular communication; however, the identity of the structural components of gap junctions in the lens has been controversial. We have cloned a novel member of the connexin family of gap junction proteins, Cx50, and have shown that it is likely...

journal_title：Molecular biology of the cell

authors： White TW,Bruzzone R,Goodenough DA,Paul DL

更新日期：1992-07-01 00:00:00

abstract：:Cell shape is regulated by cell adhesion and cytoskeletal and membrane dynamics. Cell shape, adhesion, and motility have a complex relationship and understanding them is important in understanding developmental patterning and embryogenesis. Here we show that the lipid kinase phosphatidylinositol 4-kinase III beta (PI4...

journal_title：Molecular biology of the cell

authors： Bilodeau P,Jacobsen D,Law-Vinh D,Lee JM

更新日期：2020-08-01 00:00:00

abstract：:The exocyst is an evolutionarily conserved octameric protein complex that tethers post-Golgi secretory vesicles at the plasma membrane for exocytosis. To elucidate the mechanism of vesicle tethering, it is important to understand how the exocyst physically associates with the plasma membrane (PM). In this study, we re...

journal_title：Molecular biology of the cell

authors： Liu J,Zuo X,Yue P,Guo W

更新日期：2007-11-01 00:00:00

abstract：:We studied the physiological response to limitation by diverse nutrients in batch and steady-state (chemostat) cultures of S. cerevisiae. We found that the global pattern of transcription in steady-state cultures in limiting phosphate or sulfate is essentially identical to that of batch cultures growing in the same me...

journal_title：Molecular biology of the cell

authors： Saldanha AJ,Brauer MJ,Botstein D

更新日期：2004-09-01 00:00:00

abstract：:MAL, a compact hydrophobic, four-transmembrane-domain apical protein that copurifies with detergent-resistant membranes is obligatory for the machinery that sorts glycophosphatidylinositol (GPI)-anchored proteins and others to the apical membrane in epithelia. The mechanism of MAL function in lipid-raft-mediated apica...

journal_title：Molecular biology of the cell

authors： Magal LG,Yaffe Y,Shepshelovich J,Aranda JF,de Marco Mdel C,Gaus K,Alonso MA,Hirschberg K

更新日期：2009-08-01 00:00:00

abstract：:The neonatal receptor for immunoglobulin G (IgG; FcRn) prevents IgG degradation by efficiently sorting IgG into recycling endosomes and away from lysosomes. When bound to IgG-opsonized antigen complexes, however, FcRn traffics cargo into lysosomes, where antigen processing can occur. Here we address the mechanism of s...

journal_title：Molecular biology of the cell

authors： Weflen AW,Baier N,Tang QJ,Van den Hof M,Blumberg RS,Lencer WI,Massol RH

更新日期：2013-08-01 00:00:00

abstract：:Bone morphogenetic protein (BMP) signaling pathways control many developmental and homeostatic processes, including cell size and extracellular matrix remodeling. An understanding of how this pathway itself is controlled remains incomplete. To identify novel regulators of BMP signaling, we performed a forward genetic ...

journal_title：Molecular biology of the cell

authors： Lakdawala MF,Madhu B,Faure L,Vora M,Padgett RW,Gumienny TL

更新日期：2019-12-15 00:00:00

abstract：:Many of the actions of the neuropeptide substance P (SP) that are mediated by the neurokinin 1 receptor (NK1-R) desensitize and resensitize, which may be associated with NK1-R endocytosis and recycling. We delineated this endocytic pathway in transfected cells by confocal microscopy using cyanine 3-SP and NK1-R antibo...

journal_title：Molecular biology of the cell

authors： Grady EF,Garland AM,Gamp PD,Lovett M,Payan DG,Bunnett NW

更新日期：1995-05-01 00:00:00

abstract：:The beta 2 integrin lymphocyte function-associated antigen 1 (LFA-1) mediates activation-dependent adhesion of lymphocytes. To investigate whether lymphocyte-specific elements are essential for LFA-1 function, we expressed LFA-1 in the erythroleukemic cell line K562, which expresses only the integrin very late antigen...

journal_title：Molecular biology of the cell

authors： Lub M,van Vliet SJ,Oomen SP,Pieters RA,Robinson M,Figdor CG,van Kooyk Y

更新日期：1997-04-01 00:00:00

abstract：:The tumor suppressor protein p53 mediates stress-induced growth arrest or apoptosis and plays a major role in safeguarding genome integrity. In response to DNA damage, p53 can be modified at multiple sites by phosphorylation and acetylation. We report on the characterization of p53 C-terminal phosphorylation by CHK1 a...

journal_title：Molecular biology of the cell

authors： Ou YH,Chung PH,Sun TP,Shieh SY

更新日期：2005-04-01 00:00:00

abstract：:Wounding of endothelial cells is associated with altered direct intercellular communication. To determine whether gap junctional communication participates to the wound repair process, we have compared connexin (Cx) expression, cell-to-cell coupling and kinetics of wound repair in monolayer cultures of PymT-transforme...

journal_title：Molecular biology of the cell

authors： Kwak BR,Pepper MS,Gros DB,Meda P

更新日期：2001-04-01 00:00:00

abstract：:In the budding yeast Saccharomyces cerevisiae, selection of the bud site determines the axis of polarized cell growth and eventual oriented cell division. Bud sites are selected in specific patterns depending on cell type. These patterns appear to depend on distinct types of marker proteins in the cell cortex; in part...

journal_title：Molecular biology of the cell

authors： Kang PJ,Angerman E,Nakashima K,Pringle JR,Park HO

更新日期：2004-11-01 00:00:00

abstract：:Cadherin-catenin interactions play an important role in cadherin-mediated adhesion. Here we present strong evidence that in the cadherin-catenin complex α-catenin contributes to the binding strength of another catenin, p120, to the same complex. Specifically, we found that a β-catenin-uncoupled cadherin mutant interac...

journal_title：Molecular biology of the cell

authors： Troyanovsky RB,Klingelhöfer J,Troyanovsky SM

更新日期：2011-11-01 00:00:00

abstract：:GRIM-19 was found to copurify with complex I of mitochondrial respiratory chain and subsequently was demonstrated to be involved in complex I assembly and activity. To further understand its function in complex I, we dissected its functional domains by generating a number of deletion, truncation, and point mutants. Th...

journal_title：Molecular biology of the cell

authors： Lu H,Cao X

更新日期：2008-05-01 00:00:00

abstract：:Podosomes are dynamic actin-rich structures composed of a dense F-actin core surrounded by a cloud of more diffuse F-actin. Src performs one or more unique functions in osteoclasts (OCLs), and podosome belts and bone resorption are impaired in the absence of Src. Using Src(-/-) OCLs, we investigated the specific funct...

journal_title：Molecular biology of the cell

authors： Destaing O,Sanjay A,Itzstein C,Horne WC,Toomre D,De Camilli P,Baron R

更新日期：2008-01-01 00:00:00

abstract：:Protein degradation during the cell cycle is controlled by the opposing activities of ubiquitin ligases and deubiquitinating enzymes (DUBs). Although the functions of ubiquitin ligases in the cell cycle have been studied extensively, the roles of DUBs in this process are less well understood. Here, we used an overexpr...

journal_title：Molecular biology of the cell

authors： Mapa CE,Arsenault HE,Conti MM,Poti KE,Benanti JA

更新日期：2018-11-15 00:00:00

abstract：:Rho family proteins have been implicated in regulating various cellular processes, including actin cytoskeleton organization, endocytosis, cell cycle, and gene expression. In this study, we analyzed the function of a novel Dictyostelium discoideum Rho family protein (RacC). A cell line was generated that conditionally...

journal_title：Molecular biology of the cell

authors： Seastone DJ,Lee E,Bush J,Knecht D,Cardelli J

更新日期：1998-10-01 00:00:00

abstract：:Wnt signaling plays key roles in embryonic development and adult stem cell homeostasis and is altered in human cancer. Signaling is turned on and off by regulating stability of the effector β-catenin (β-cat). The multiprotein destruction complex binds and phosphorylates β-cat and transfers it to the SCF-TrCP E3-ubiqui...

journal_title：Molecular biology of the cell

authors： Schaefer KN,Pronobis MI,Williams CE,Zhang S,Bauer L,Goldfarb D,Yan F,Major MB,Peifer M

更新日期：2020-05-01 00:00:00

abstract：:Guanine nucleotide exchange factors (GEFs) are the initiators of signaling by every regulatory GTPase, which in turn act to regulate a wide array of essential cellular processes. To date, each family of GTPases is activated by distinct families of GEFs. Bidirectional membrane trafficking is regulated by ADP-ribosylati...

journal_title：Molecular biology of the cell

authors： Pipaliya SV,Schlacht A,Klinger CM,Kahn RA,Dacks J

更新日期：2019-07-15 00:00:00

abstract：:In response to engagement of surface molecules, cells generate active forces that regulate many cellular processes. Developing tools that permit gathering mechanical and morphological information on these forces is of the utmost importance. Here we describe a new technique, the micropipette force probe, that uses a mi...

journal_title：Molecular biology of the cell

authors： Sawicka A,Babataheri A,Dogniaux S,Barakat AI,Gonzalez-Rodriguez D,Hivroz C,Husson J

更新日期：2017-11-07 00:00:00

abstract：:Apoptosis causes characteristic morphological changes in cells, including membrane blebbing, cell detachment from the extracellular matrix, and loss of cell-cell contacts. We investigated the changes in focal adhesion proteins during etoposide-induced apoptosis in Rat-1 cells and found that during apoptosis, p130cas (...

journal_title：Molecular biology of the cell

authors： Kook S,Shim SR,Choi SJ,Ahnn J,Kim JI,Eom SH,Jung YK,Paik SG,Song WK

更新日期：2000-03-01 00:00:00

abstract：:B-type cyclins are rapidly degraded at the transition between metaphase and anaphase and their ubiquitin-mediated proteolysis is required for cells to exit mitosis. We used a novel enrichment to isolate new budding mutants that arrest the cell cycle in mitosis. Most of these mutants lie in the CDC16, CDC23, and CDC27 ...

journal_title：Molecular biology of the cell

authors： Hwang LH,Murray AW

更新日期：1997-10-01 00:00:00

abstract：:Clearance of misfolded proteins from the ER is central for maintenance of cellular homeostasis. This process requires coordinated recognition, ER-cytosol translocation, and finally ubiquitination-dependent proteasomal degradation. Here, we identify an ER resident seven-transmembrane protein (JAMP) that links ER chaper...

journal_title：Molecular biology of the cell

authors： Tcherpakov M,Broday L,Delaunay A,Kadoya T,Khurana A,Erdjument-Bromage H,Tempst P,Qiu XB,DeMartino GN,Ronai Z

更新日期：2008-11-01 00:00:00

abstract：:A cornerstone of the antiviral interferon response is phosphorylation of eukaryotic initiation factor (eIF)2alpha. This limits the availability of eIF2.GTP.Met-tRNA(i)(Met) ternary complexes, reduces formation of 43S preinitiation complexes, and blocks viral (and most cellular) mRNA translation. However, many viruses ...

journal_title：Molecular biology of the cell

authors： Robert F,Kapp LD,Khan SN,Acker MG,Kolitz S,Kazemi S,Kaufman RJ,Merrick WC,Koromilas AE,Lorsch JR,Pelletier J

更新日期：2006-11-01 00:00:00

abstract：:The nuclear envelope of metazoans disassembles during mitosis and reforms in late anaphase after sister chromatids have well separated. The coordination of these mitotic events is important for genome stability, yet the temporal control of nuclear envelope reassembly is unknown. Although the steps of nuclear formation...

journal_title：Molecular biology of the cell

authors： Tseng LC,Chen RH

更新日期：2011-09-01 00:00:00

abstract：:Several membrane-associating signals, including covalently linked fatty acids, are found in various combinations at the N termini of signaling proteins. The function of these combinations was investigated by appending fatty acylated N-terminal sequences to green fluorescent protein (GFP). Myristoylated plus mono/dipal...

journal_title：Molecular biology of the cell

authors： McCabe JB,Berthiaume LG

更新日期：1999-11-01 00:00:00

abstract：:Kinesin-8 motor proteins destabilize microtubules. Their absence during cell division is associated with disorganized mitotic chromosome movements and chromosome loss. Despite recent work studying effects of kinesin-8s on microtubule dynamics, it remains unclear whether the kinesin-8 mitotic phenotypes are consequence...

journal_title：Molecular biology of the cell

authors： Gergely ZR,Crapo A,Hough LE,McIntosh JR,Betterton MD

更新日期：2016-11-07 00:00:00

abstract：:The formation and maintenance of the bipolar mitotic spindle apparatus require a complex and balanced interplay of several mechanisms, including the stabilization and separation of polar microtubules and the action of various microtubule motors. Nonmicrotubule elements are also present throughout the spindle apparatus...

journal_title：Molecular biology of the cell

authors： Yang CH,Snyder M

更新日期：1992-11-01 00:00:00