Abstract:
:The maturation of dendritic cells is accompanied by the redistribution of major histocompatibility complex (MHC) class II molecules from the lysosomal MHC class II compartment to the plasma membrane to mediate presentation of peptide antigens. Besides MHC molecules, dendritic cells also express CD1 molecules that mediate presentation of lipid antigens. Herein, we show that in human monocyte-derived dendritic cells, unlike MHC class II, the steady-state distribution of lysosomal CD1b and CD1c isoforms was unperturbed in response to lipopolysaccharide-induced maturation. However, the lysosomes in these cells underwent a dramatic reorganization into electron dense tubules with altered lysosomal protein composition. These structures matured into novel and morphologically unique compartments, here termed mature dendritic cell lysosomes (MDL). Furthermore, we show that upon activation mature dendritic cells do not lose their ability of efficient clathrin-mediated endocytosis as demonstrated for CD1b and transferrin receptor molecules. Thus, the constitutive endocytosis of CD1b molecules and the differential sorting of MHC class II from lysosomes separate peptide- and lipid antigen-presenting molecules during dendritic cell maturation.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
van der Wel NN,Sugita M,Fluitsma DM,Cao X,Schreibelt G,Brenner MB,Peters PJdoi
10.1091/mbc.e02-11-0744keywords:
subject
Has Abstractpub_date
2003-08-01 00:00:00pages
3378-88issue
8eissn
1059-1524issn
1939-4586pii
E02-11-0744journal_volume
14pub_type
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