Correlation of CRM1-NES affinity with nuclear export activity.

abstract：:Proteins establish and maintain a distinct intracellular localization by means of targeting, retention, and retrieval signals, ensuring most proteins reside predominantly in one cellular location. The enzymes involved in the maturation of lamin A present a challenge to this paradigm. Lamin A is first synthesized as a ...

journal_title：Molecular biology of the cell

authors： Barrowman J,Hamblet C,George CM,Michaelis S

更新日期：2008-12-01 00:00:00

abstract：:GM3 synthase (SAT-I) is the primary glycosyltransferase responsible for the biosynthesis of ganglio-series gangliosides. In this study, we identify three isoforms of mouse SAT-I proteins, named M1-SAT-I, M2-SAT-I, and M3-SAT-I, which possess distinct lengths in their NH(2)-terminal cytoplasmic tails. These isoforms ar...

journal_title：Molecular biology of the cell

authors： Uemura S,Yoshida S,Shishido F,Inokuchi J

更新日期：2009-07-01 00:00:00

abstract：:The plant toxin ricin is transported retrogradely from the cell surface to the endoplasmic reticulum (ER) from where the enzymatically active part is retrotranslocated to the cytosol, presumably by the same mechanism as used by misfolded proteins. The ER degradation enhancing alpha-mannosidase I-like protein, EDEM, is...

journal_title：Molecular biology of the cell

authors： Slominska-Wojewodzka M,Gregers TF,Wälchli S,Sandvig K

更新日期：2006-04-01 00:00:00

abstract：:Motor neuron (MN) diseases are progressive disorders resulting from degeneration of neuromuscular junctions (NMJs), which form the connection between MNs and muscle fibers. NMJ-in-a-dish models have been developed to examine human MN-associated dysfunction with disease; however such coculture models have randomly orie...

journal_title：Molecular biology of the cell

authors： Happe CL,Tenerelli KP,Gromova AK,Kolb F,Engler AJ

更新日期：2017-07-07 00:00:00

abstract：:In animal cells, positioning of the mitotic spindle is crucial for defining the plane of cytokinesis and the size ratio of daughter cells. We have characterized this phenomenon in a rat epithelial cell line using microscopy, micromanipulation, and microinjection. Unmanipulated cells position the mitotic spindle near t...

journal_title：Molecular biology of the cell

authors： O'Connell CB,Wang YL

更新日期：2000-05-01 00:00:00

abstract：:The zona pellucida (ZP) is a highly organized extracellular coat that surrounds all mammalian eggs. The mouse egg ZP is composed of three glycoproteins, called mZP1-3, that are synthesized, secreted, and assembled into a ZP exclusively by growing oocytes. Here, we microinjected epitope-tagged (Myc and Flag) cDNAs for ...

journal_title：Molecular biology of the cell

authors： Qi H,Williams Z,Wassarman PM

更新日期：2002-02-01 00:00:00

abstract：:Although there is considerable evidence implicating posttranslational mechanisms in the development of epithelial cell polarity, little is known about the patterns of gene expression and transcriptional regulation during this process. We characterized the temporal program of gene expression during cell-cell adhesion-i...

journal_title：Molecular biology of the cell

authors： Halbleib JM,Sääf AM,Brown PO,Nelson WJ

更新日期：2007-11-01 00:00:00

abstract：:Nuclear pore complexes consist of several subcomplexes. The NUP107 complex is important for nucleocytoplasmic transport, nuclear envelope assembly, and kinetochore function. However, the underlying molecular mechanisms and the roles of individual complex members remain elusive. We report the first description of a gen...

journal_title：Molecular biology of the cell

authors： Ródenas E,González-Aguilera C,Ayuso C,Askjaer P

更新日期：2012-03-01 00:00:00

abstract：:The Sec61 translocon mediates the translocation of proteins across the endoplasmic reticulum membrane and the lateral integration of transmembrane segments into the lipid bilayer. The structure of the idle translocon is closed by a lumenal plug domain and a hydrophobic constriction ring. To test the function of the ap...

journal_title：Molecular biology of the cell

authors： Junne T,Kocik L,Spiess M

更新日期：2010-05-15 00:00:00

abstract：:In all eucaryotic cell types analyzed, proliferations of the endoplasmic reticulum (ER) can be induced by increasing the levels of certain integral ER proteins. One of the best characterized of these proteins is HMG-CoA reductase, which catalyzes the rate-limiting step in sterol biosynthesis. We have investigated the ...

journal_title：Molecular biology of the cell

authors： Koning AJ,Roberts CJ,Wright RL

更新日期：1996-05-01 00:00:00

abstract：:Clathrin-mediated endocytosis and phagocytosis are both selective surface internalization processes but have little known mechanistic similarity or interdependence. Here we show that the phosphotyrosine-binding (PTB) domain protein Ced-6, a well-established phagocytosis component that operates as a transducer of so-ca...

journal_title：Molecular biology of the cell

authors： Jha A,Watkins SC,Traub LM

更新日期：2012-05-01 00:00:00

abstract：:We have recently shown that the pancreatic bile salt-dependent lipase (BSDL) can be taken up by intestinal cells and transported to the blood circulation. This mechanism likely involves (specific) receptor(s) able to bind BSDL and located at the apical intestinal cell membrane. In this study, using Int407 human intest...

journal_title：Molecular biology of the cell

authors： Bruneau N,Richard S,Silvy F,Verine A,Lombardo D

更新日期：2003-07-01 00:00:00

abstract：:When Candida albicans yeast cells receive the appropriate stimulus, they switch to hyphal growth, characterized by continuous apical elongation and the inhibition of cell separation. The molecular basis of this inhibition is poorly known, despite its crucial importance for hyphal development. In C. albicans, septins a...

journal_title：Molecular biology of the cell

authors： González-Novo A,Correa-Bordes J,Labrador L,Sánchez M,Vázquez de Aldana CR,Jiménez J

更新日期：2008-04-01 00:00:00

abstract：:Hedgehog (Hh) is a secreted morphogen involved in both short- and long-range signaling necessary for tissue patterning during development. It is unclear how this dually lipidated protein is transported over a long range in the aqueous milieu of interstitial spaces. We previously showed that the long-range signaling of...

journal_title：Molecular biology of the cell

authors： Parchure A,Vyas N,Ferguson C,Parton RG,Mayor S

更新日期：2015-12-15 00:00:00

abstract：:Steroids and growth factors control neuronal development through their receptors under physiological and pathological conditions. We show that PC12 cells harbor endogenous androgen receptor (AR), whose inhibition or silencing strongly interferes with neuritogenesis stimulated by the nonaromatizable synthetic androgen ...

journal_title：Molecular biology of the cell

authors： Di Donato M,Bilancio A,D'Amato L,Claudiani P,Oliviero MA,Barone MV,Auricchio A,Appella E,Migliaccio A,Auricchio F,Castoria G

更新日期：2015-08-01 00:00:00

abstract：:We conducted a genetic screen for mutations in myospheroid, the gene encoding the Drosophila betaPS integrin subunit, and identified point mutants in all of the structural domains of the protein. Surprisingly, we find that mutations in very strongly conserved residues will often allow sufficient integrin function to s...

journal_title：Molecular biology of the cell

authors： Jannuzi AL,Bunch TA,West RF,Brower DL

更新日期：2004-08-01 00:00:00

abstract：:SLC45A2 encodes a putative transporter expressed primarily in pigment cells. SLC45A2 mutations cause oculocutaneous albinism type 4 (OCA4) and polymorphisms are associated with pigmentation variation, but the localization, function, and regulation of SLC45A2 and its variants remain unknown. We show that SLC45A2 locali...

journal_title：Molecular biology of the cell

authors： Le L,Escobar IE,Ho T,Lefkovith AJ,Latteri E,Haltaufderhyde KD,Dennis MK,Plowright L,Sviderskaya EV,Bennett DC,Oancea E,Marks MS

更新日期：2020-11-15 00:00:00

abstract：:We have previously identified a novel family of proteins called the GGAs (Golgi-localized, gamma-ear-containing, ADP-ribosylation factor-binding proteins). These proteins consist of an NH(2)-terminal VHS domain, followed by a GAT domain, a variable domain, and a gamma-adaptin ear homology domain. Studies from our own ...

journal_title：Molecular biology of the cell

authors： Hirst J,Lindsay MR,Robinson MS

更新日期：2001-11-01 00:00:00

abstract：:SPOP, speckle-type POZ protein is a substrate adaptor protein of the Cullin-3/RING ubiquitin E3 complex. The spop gene is the most commonly point mutated in human primary prostate cancers, but the pathological contribution of the SPOP mutations remains unclear. In this study, we investigated several known factors that...

journal_title：Molecular biology of the cell

authors： Watanabe R,Maekawa M,Hieda M,Taguchi T,Miura N,Kikugawa T,Saika T,Higashiyama S

更新日期：2020-03-15 00:00:00

abstract：:Keratins are cytoplasmic intermediate filament proteins preferentially expressed by epithelial tissues in a site-specific and differentiation-dependent manner. The complex network of keratin filaments in stratified epithelia is tightly regulated during squamous cell differentiation. Keratin 14 (K14) is expressed in mi...

journal_title：Molecular biology of the cell

authors： Alam H,Sehgal L,Kundu ST,Dalal SN,Vaidya MM

更新日期：2011-11-01 00:00:00

abstract：:Regulator of G protein signaling (RGS) proteins inhibit G protein signaling by activating Galpha GTPase activity, but the mechanisms that regulate RGS activity are not well understood. The mammalian R7 binding protein (R7BP) can interact with all members of the R7 family of RGS proteins, and palmitoylation of R7BP can...

journal_title：Molecular biology of the cell

authors： Porter MY,Koelle MR

更新日期：2010-01-15 00:00:00

abstract：:We report here that Trypanosoma cruzi, the etiologic agent of Chagas disease, possesses two unique paralogues of the mitochondrial calcium uniporter complex TcMCU subunit that we named TcMCUc and TcMCUd. The predicted structure of the proteins indicates that, as predicted for the TcMCU and TcMCUb paralogues, they are ...

journal_title：Molecular biology of the cell

authors： Chiurillo MA,Lander N,Bertolini MS,Vercesi AE,Docampo R

更新日期：2019-07-01 00:00:00

abstract：:Phosphoinositides (PIs) regulate a myriad of cellular functions including membrane fusion, as exemplified by the yeast vacuole, which uses various PIs at different stages of fusion. In light of this, the effect of phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) on vacuole fusion remains unknown. PI(3,5)P2 is made by...

journal_title：Molecular biology of the cell

authors： Miner GE,Sullivan KD,Guo A,Jones BC,Hurst LR,Ellis EC,Starr ML,Fratti RA

更新日期：2019-01-15 00:00:00

abstract：:Septins are members of a highly conserved family of filamentous proteins that are required in many organisms for the completion of cytokinesis. In addition, septins have been implicated in a number of important cellular processes and have been suggested to have roles in regulating membrane traffic. Given the proposed ...

journal_title：Molecular biology of the cell

authors： Huang YW,Yan M,Collins RF,Diciccio JE,Grinstein S,Trimble WS

更新日期：2008-04-01 00:00:00

abstract：:A genome-wide screen of 4168 homozygous diploid yeast deletion strains has been performed to identify nonessential genes that participate in the bipolar budding pattern. By examining bud scar patterns representing the sites of previous cell divisions, 127 mutants representing three different phenotypes were found: uni...

journal_title：Molecular biology of the cell

authors： Ni L,Snyder M

更新日期：2001-07-01 00:00:00

abstract：:Although evasion of apoptosis is thought to be required for the development of cancer, it is unclear which cell death pathways are evaded. We previously identified a novel epithelial cell death pathway that works in normal cells but is inactivated in tumor cells, implying that it may be targeted during tumor developme...

journal_title：Molecular biology of the cell

authors： Thorburn J,Moore F,Rao A,Barclay WW,Thomas LR,Grant KW,Cramer SD,Thorburn A

更新日期：2005-03-01 00:00:00

abstract：:To test the "traction fiber" model for metaphase positioning of bivalents during meiosis, kinetochore fibers of maloriented bivalents, induced during recovery from cold arrest, were analyzed with a liquid crystal polarizing microscope. The measured birefringence retardation of kinetochore fibers is proportional to the...

journal_title：Molecular biology of the cell

authors： LaFountain JR Jr,Oldenbourg R

更新日期：2004-12-01 00:00:00

abstract：:Nuclear FGFR1 acts as a developmental gene regulator in cooperation with FGF-2, RSK1, and CREB-binding protein (CBP). FRAP analysis revealed three nuclear FGFR1 populations: i) a fast mobile, ii) a slower mobile population reflecting chromatin-bound FGFR1, and iii) an immobile FGFR1 population associated with the nucl...

journal_title：Molecular biology of the cell

authors： Dunham-Ems SM,Lee YW,Stachowiak EK,Pudavar H,Claus P,Prasad PN,Stachowiak MK

更新日期：2009-05-01 00:00:00

abstract：:Members of the adenine nucleotide translocase (ANT) family exchange ADP for ATP across the mitochondrial inner membrane, an activity that is essential for oxidative phosphorylation (OXPHOS). Mutations in or dysregulation of ANTs is associated with progressive external ophthalmoplegia, cardiomyopathy, nonsyndromic inte...

journal_title：Molecular biology of the cell

authors： Lu YW,Acoba MG,Selvaraju K,Huang TC,Nirujogi RS,Sathe G,Pandey A,Claypool SM

更新日期：2017-06-01 00:00:00

abstract：:Growth cone responses to guidance cues provide the basis for neuronal pathfinding. Although many cues have been identified, less is known about how signals are translated into the cytoskeletal rearrangements that steer directional changes during pathfinding. Here we show that the response of dorsal root ganglion (DRG)...

journal_title：Molecular biology of the cell

authors： Brown JA,Wysolmerski RB,Bridgman PC

更新日期：2009-02-01 00:00:00