Phosphatidylinositol 3,5-bisphosphate regulates the transition between trans-SNARE complex formation and vacuole membrane fusion.

abstract：:The efficient activation of p90(rsk) by MAP kinase requires their interaction through a docking site located at the C-terminal end of p90(rsk). The MAP kinase p42(mpk1) can associate with p90(rsk) in G(2)-arrested but not in mature Xenopus oocytes. In contrast, an N-terminally truncated p90(rsk) mutant named D2 consti...

journal_title：Molecular biology of the cell

authors： Gavin AC,Ni Ainle A,Chierici E,Jones M,Nebreda AR

更新日期：1999-09-01 00:00:00

abstract：:In this essay I describe my personal journey from reductionist to systems cell biology and describe how this in turn led to a 3-year sea voyage to explore complex ocean communities. In describing this journey, I hope to convey some important principles that I gleaned along the way. I realized that cellular functions e...

journal_title：Molecular biology of the cell

authors： Karsenti E

更新日期：2012-07-01 00:00:00

abstract：:We studied the physiological response to limitation by diverse nutrients in batch and steady-state (chemostat) cultures of S. cerevisiae. We found that the global pattern of transcription in steady-state cultures in limiting phosphate or sulfate is essentially identical to that of batch cultures growing in the same me...

journal_title：Molecular biology of the cell

authors： Saldanha AJ,Brauer MJ,Botstein D

更新日期：2004-09-01 00:00:00

abstract：:Cell cycle-dependent morphogenesis of unicellular organisms depends on the spatiotemporal control of cell polarity. Rho GTPases are the major players that guide cells through structural reorganizations such as cytokinesis (Rho1 dependent) and polarity establishment (Cdc42 dependent). In budding yeast, the protein Gps1...

journal_title：Molecular biology of the cell

authors： Meitinger F,Pereira G

更新日期：2017-04-01 00:00:00

abstract：:Prohibitins comprise a remarkably conserved protein family in eukaryotic cells with proposed functions in cell cycle progression, senescence, apoptosis, and the regulation of mitochondrial activities. Two prohibitin homologues, Phb1 and Phb2, assemble into a high molecular weight complex of approximately 1.2 MDa in th...

journal_title：Molecular biology of the cell

authors： Tatsuta T,Model K,Langer T

更新日期：2005-01-01 00:00:00

abstract：:Myosin II-dependent contraction of the contractile ring drives equatorial furrowing during cytokinesis in animal cells. Nonetheless, myosin II-null cells of the cellular slime mold Dictyostelium divide efficiently when adhering to substrates by making use of polar traction forces. Here, we show that in the presence of...

journal_title：Molecular biology of the cell

authors： Kanada M,Nagasaki A,Uyeda TQ

更新日期：2005-08-01 00:00:00

abstract：:Secretory proteins are exported from the endoplasmic reticulum in COPII vesicles. SNARE proteins-core machinery for membrane fusion-are incorporated into COPII vesicles by direct interaction with Sec24. Here we report a novel mechanism for sorting of the ER-Golgi Q-SNAREs into COPII vesicles. Different mammalian Sec24...

journal_title：Molecular biology of the cell

authors： Adolf F,Rhiel M,Reckmann I,Wieland FT

更新日期：2016-09-01 00:00:00

abstract：:The subcellular distribution of kinases and other signaling proteins is regulated in response to cellular cues; however, the extent of this regulation has not been investigated for any gene set in any organism. Here, we present a systematic analysis of protein kinases in the budding yeast, screening for differential l...

journal_title：Molecular biology of the cell

authors： Bharucha N,Ma J,Dobry CJ,Lawson SK,Yang Z,Kumar A

更新日期：2008-07-01 00:00:00

abstract：:The stem-loop binding protein (SLBP1) binds the 3' stem-loop of histone pre-mRNA and is required for efficient processing of histone transcripts in the nucleus. We examined the localization of SLBP1 in the germinal vesicle of Xenopus laevis oocytes. In spread preparations of germinal vesicle contents, an anti-SLBP1 an...

journal_title：Molecular biology of the cell

authors： Abbott J,Marzluff WF,Gall JG

更新日期：1999-02-01 00:00:00

abstract：:Formation of multiple-protein macromolecular complexes at specialized subcellular microdomains increases the specificity and efficiency of signaling in cells. In this study, we demonstrate that phosphodiesterase type 3A (PDE3A) physically and functionally interacts with cystic fibrosis transmembrane conductance regula...

journal_title：Molecular biology of the cell

authors： Penmatsa H,Zhang W,Yarlagadda S,Li C,Conoley VG,Yue J,Bahouth SW,Buddington RK,Zhang G,Nelson DJ,Sonecha MD,Manganiello V,Wine JJ,Naren AP

更新日期：2010-03-15 00:00:00

abstract：:Eukaryotic cell survival requires maintenance of plasma membrane (PM) homeostasis in response to environmental insults and changes in lipid metabolism. In yeast, a key regulator of PM homeostasis is target of rapamycin (TOR) complex 2 (TORC2), a multiprotein complex containing the evolutionarily conserved TOR protein ...

journal_title：Molecular biology of the cell

authors： Martinez Marshall MN,Emmerstorfer-Augustin A,Leskoske KL,Zhang LH,Li B,Thorner J

更新日期：2019-06-01 00:00:00

abstract：:Ras signaling has been shown to play an important role in promoting cell survival in many different tissues. Here we show that upregulation of Ras activity in adult Drosophila neurons induces neuronal cell death, as evident from the phenotype of vacuolar peduncle (vap) mutants defective in the Drosophila RasGAP gene, ...

journal_title：Molecular biology of the cell

authors： Botella JA,Kretzschmar D,Kiermayer C,Feldmann P,Hughes DA,Schneuwly S

更新日期：2003-01-01 00:00:00

abstract：:We identified a direct interaction between the neuronal transmembrane protein calsyntenin-1 and the light chain of Kinesin-1 (KLC1). GST pulldowns demonstrated that two highly conserved segments in the cytoplasmic domain of calsyntenin-1 mediate binding to the tetratricopeptide repeats of KLC1. A complex containing ca...

journal_title：Molecular biology of the cell

authors： Konecna A,Frischknecht R,Kinter J,Ludwig A,Steuble M,Meskenaite V,Indermühle M,Engel M,Cen C,Mateos JM,Streit P,Sonderegger P

更新日期：2006-08-01 00:00:00

abstract：:Cell surface hemichannels (HCs) composed of different connexin (Cx) types are present in diverse cells and their possible role on FGF-1-induced cellular responses remains unknown. Here, we show that FGF-1 transiently (4-14 h, maximal at 7 h) increases the membrane permeability through HCs in HeLa cells expressing Cx43...

journal_title：Molecular biology of the cell

authors： Schalper KA,Palacios-Prado N,Retamal MA,Shoji KF,Martínez AD,Sáez JC

更新日期：2008-08-01 00:00:00

abstract：:Proteins establish and maintain a distinct intracellular localization by means of targeting, retention, and retrieval signals, ensuring most proteins reside predominantly in one cellular location. The enzymes involved in the maturation of lamin A present a challenge to this paradigm. Lamin A is first synthesized as a ...

journal_title：Molecular biology of the cell

authors： Barrowman J,Hamblet C,George CM,Michaelis S

更新日期：2008-12-01 00:00:00

abstract：:The secretory and autophagy pathways can be thought of as the biosynthetic (i.e., anabolic) and degradative (i.e., catabolic) branches of the endomembrane system. In analogy to anabolic and catabolic pathways in metabolism, there is mounting evidence that the secretory and autophagy pathways are intimately linked and ...

journal_title：Molecular biology of the cell

authors： Farhan H,Kundu M,Ferro-Novick S

更新日期：2017-05-01 00:00:00

abstract：:Stress fibers were isolated from cultured human foreskin fibroblasts and bovine endothelial cells, and their contraction was demonstrated in vitro. Cells in culture dishes were first treated with a low-ionic-strength extraction solution and then further extracted using detergents. With gentle washes by pipetting, the ...

journal_title：Molecular biology of the cell

authors： Katoh K,Kano Y,Masuda M,Onishi H,Fujiwara K

更新日期：1998-07-01 00:00:00

abstract：:During the cell cycle, Cdc2-cyclin B kinase abruptly becomes active and triggers the entry into mitosis/meiosis. Recently, it was found that inactive Cdc2-cyclin B is present in aggregates in immature starfish oocytes and becomes disaggregated at the time of its activation during maturation. We discuss a possible scen...

journal_title：Molecular biology of the cell

authors： Slepchenko BM,Terasaki M

更新日期：2003-11-01 00:00:00

abstract：:Dysregulated hepatic cholesterol homeostasis with free cholesterol accumulation in the liver is relevant to the pathogenesis of nonalcoholic steatohepatitis, contributing to the chronicity of liver toxicity. Here we examined the effect of free cholesterol accumulation on the morphology and biochemical properties of li...

journal_title：Molecular biology of the cell

authors： Makino A,Hullin-Matsuda F,Murate M,Abe M,Tomishige N,Fukuda M,Yamashita S,Fujimoto T,Vidal H,Lagarde M,Delton I,Kobayashi T

更新日期：2016-11-01 00:00:00

abstract：:Integrins are major receptors used by cells to interact with extracellular matrices. In this paper, we identify the first ligands for the beta 8 family of integrins, presenting evidence that integrin heterodimers containing the beta 8 subunit mediate interactions of chick sensory neurons with laminin-1, collagen IV, a...

journal_title：Molecular biology of the cell

authors： Venstrom K,Reichardt L

更新日期：1995-04-01 00:00:00

abstract：:During vertebrate neuromuscular junction (NMJ) assembly, motor axons and their muscle targets exchange short-range signals that regulate the subsequent steps of presynaptic and postsynaptic specialization. We report here that this interaction is in part mediated by axonal filopodia extended preferentially by cultured ...

journal_title：Molecular biology of the cell

authors： Li PP,Chen C,Lee CW,Madhavan R,Peng HB

更新日期：2011-07-15 00:00:00

abstract：:Fibroblasts, when plated on the extracellular matrix protein fibronectin (FN), rapidly spread and form an organized actin cytoskeleton. This process is known to involve both the central alpha5beta1 integrin-binding and the C-terminal heparin-binding regions of FN. We found that within the heparin-binding region, the i...

journal_title：Molecular biology of the cell

authors： Bloom L,Ingham KC,Hynes RO

更新日期：1999-05-01 00:00:00

abstract：:Clearance of misfolded proteins from the ER is central for maintenance of cellular homeostasis. This process requires coordinated recognition, ER-cytosol translocation, and finally ubiquitination-dependent proteasomal degradation. Here, we identify an ER resident seven-transmembrane protein (JAMP) that links ER chaper...

journal_title：Molecular biology of the cell

authors： Tcherpakov M,Broday L,Delaunay A,Kadoya T,Khurana A,Erdjument-Bromage H,Tempst P,Qiu XB,DeMartino GN,Ronai Z

更新日期：2008-11-01 00:00:00

abstract：:Cellular differentiation and lineage commitment are considered to be robust and irreversible processes during development. Recent work has shown that mouse and human fibroblasts can be reprogrammed to a pluripotent state with a combination of four transcription factors. We hypothesized that combinatorial expression of...

journal_title：Molecular biology of the cell

authors： Ishii R,Kami D,Toyoda M,Makino H,Gojo S,Ishii T,Umezawa A

更新日期：2012-09-01 00:00:00

abstract：:The reovirus fusion-associated small transmembrane (FAST) proteins comprise a unique family of viral membrane fusion proteins dedicated to inducing cell-cell fusion. We recently reported that a polybasic motif (PBM) in the cytosolic tail of reptilian reovirus p14 FAST protein functions as a novel tribasic Golgi export...

journal_title：Molecular biology of the cell

authors： Parmar HB,Duncan R

更新日期：2016-04-15 00:00:00

abstract：:The Rho GTPases RhoA and Rac1 function as master regulators of cytokinesis by controlling the actomyosin cytoskeleton. RhoA and Rac1 have to be respectively activated and inactivated at the division plane for cytokinesis to occur properly. The inactivation of Rac1 at the cleavage furrow is controlled by MgcRacGAP. How...

journal_title：Molecular biology of the cell

authors： Cannet A,Schmidt S,Delaval B,Debant A

更新日期：2014-12-15 00:00:00

abstract：:We conducted a genetic screen for mutations in myospheroid, the gene encoding the Drosophila betaPS integrin subunit, and identified point mutants in all of the structural domains of the protein. Surprisingly, we find that mutations in very strongly conserved residues will often allow sufficient integrin function to s...

journal_title：Molecular biology of the cell

authors： Jannuzi AL,Bunch TA,West RF,Brower DL

更新日期：2004-08-01 00:00:00

abstract：:Insulin and hypertonicity each increase the content of GLUT4 glucose transporters at the surface of muscle cells. Insulin enhances GLUT4 exocytosis without diminishing its endocytosis. The insulin but not the hypertonicity response is reduced by tetanus neurotoxin, which cleaves vesicle-associated membrane protein (VA...

journal_title：Molecular biology of the cell

authors： Randhawa VK,Thong FS,Lim DY,Li D,Garg RR,Rudge R,Galli T,Rudich A,Klip A

更新日期：2004-12-01 00:00:00

abstract：:In view of the common regulatory mechanism that induces transcription of the mitotic phosphatase cdc25C and cyclin A at the beginning of S-phase, we investigated whether cdc25C was required for S-phase transit. Here, we show that in both nontransformed human fibroblasts and HeLa cells, cdc25C protein levels significan...

journal_title：Molecular biology of the cell

authors： Turowski P,Franckhauser C,Morris MC,Vaglio P,Fernandez A,Lamb NJ

更新日期：2003-07-01 00:00:00

abstract：:Cell adhesion to extracellular matrix molecules such as fibronectin involves complex transmembrane signaling processes. Attachment and spreading of primary fibroblasts can be promoted by interactions of cell surface integrins with RGD-containing fragments of fibronectin, but the further process of focal adhesion and s...

journal_title：Molecular biology of the cell

authors： Woods A,McCarthy JB,Furcht LT,Couchman JR

更新日期：1993-06-01 00:00:00