Formation of membrane-bound ring complexes by prohibitins in mitochondria.

abstract：:Many mitochondrial outer membrane (MOM) proteins have a transmembrane domain near the C terminus and an N-terminal cytosolic moiety. It is not clear how these tail-anchored (TA) proteins posttranslationally select their target, but C-terminal charged residues play an important role. To investigate how discrimination b...

journal_title：Molecular biology of the cell

authors： Borgese N,Gazzoni I,Barberi M,Colombo S,Pedrazzini E

更新日期：2001-08-01 00:00:00

abstract：:MPM-2 antigens, a discrete set of phosphoproteins that contain similar phosphoepitopes recognized by the monoclonal antibody MPM-2, are phosphorylated during M-phase induction. Our previous studies suggested that certain MPM-2 antigens are involved in the appearance of maturation-promoting factor (MPF) activity. Becau...

journal_title：Molecular biology of the cell

authors： Kuang J,Ashorn CL,Gonzalez-Kuyvenhoven M,Penkala JE

更新日期：1994-02-01 00:00:00

abstract：:Based on its shedding and binding activities, the disintegrin and metalloprotease 12 (ADAM12) has been implicated in cell signaling. Here we investigate the intracellular protein interaction network of the transmembrane ADAM12L variant using an integrative approach. We identify the integrin-linked kinase (ILK) as a ne...

journal_title：Molecular biology of the cell

authors： Leyme A,Bourd-Boittin K,Bonnier D,Falconer A,Arlot-Bonnemains Y,Théret N

更新日期：2012-09-01 00:00:00

abstract：:Hedgehog (Hh) is a secreted morphogen involved in both short- and long-range signaling necessary for tissue patterning during development. It is unclear how this dually lipidated protein is transported over a long range in the aqueous milieu of interstitial spaces. We previously showed that the long-range signaling of...

journal_title：Molecular biology of the cell

authors： Parchure A,Vyas N,Ferguson C,Parton RG,Mayor S

更新日期：2015-12-15 00:00:00

abstract：:Transport of cellular and neuronal vesicles, organelles, and other particles along microtubules requires the molecular motor protein dynein (Mallik and Gross, 2004). Critical to dynein function is dynactin, a multiprotein complex commonly thought to be required for dynein attachment to membrane compartments (Karki and...

journal_title：Molecular biology of the cell

authors： Haghnia M,Cavalli V,Shah SB,Schimmelpfeng K,Brusch R,Yang G,Herrera C,Pilling A,Goldstein LS

更新日期：2007-06-01 00:00:00

abstract：:The mmd1 mutation causes temperature-sensitive growth and defects in mitochondrial morphology and distribution in the fission yeast Schizosaccharomyces pombe. In mutant cells, mitochondria aggregate at the two cell ends, with increased aggregation at elevated temperatures. Microtubules, which mediate mitochondrial pos...

journal_title：Molecular biology of the cell

authors： Weir BA,Yaffe MP

更新日期：2004-04-01 00:00:00

abstract：:In this study, we examined the subcellular distribution and functions of LIMK1 in developing neurons. Confocal microscopy, subcellular fractionation, and expression of several epitope-tagged LIMK1 constructs revealed that LIMK1 is enriched in the Golgi apparatus and growth cones, with the LIM domain required for Golgi...

journal_title：Molecular biology of the cell

authors： Rosso S,Bollati F,Bisbal M,Peretti D,Sumi T,Nakamura T,Quiroga S,Ferreira A,Cáceres A

更新日期：2004-07-01 00:00:00

abstract：:The Golgi apparatus consists of a series of flattened cisternal membranes that are aligned in parallel to form stacks. Cytosolic-oriented Golgi-associated proteins have been identified that may coordinate or maintain the Golgi architecture. Here, we describe a novel GPI-anchored protein, Golgi-resident GPI-anchored pr...

journal_title：Molecular biology of the cell

authors： Li X,Kaloyanova D,van Eijk M,Eerland R,van der Goot G,Oorschot V,Klumperman J,Lottspeich F,Starkuviene V,Wieland FT,Helms JB

更新日期：2007-04-01 00:00:00

abstract：:Microtubules (MTs) in newt mitotic spindles grow faster than MTs in the interphase cytoplasmic microtubule complex (CMTC), yet spindle MTs do not have the long lengths or lifetimes of the CMTC microtubules. Because MTs undergo dynamic instability, it is likely that changes in the durations of growth or shortening are ...

journal_title：Molecular biology of the cell

authors： Gliksman NR,Skibbens RV,Salmon ED

更新日期：1993-10-01 00:00:00

abstract：:Eukaryotic cells eliminate misfolded proteins from the endoplasmic reticulum (ER) via a conserved process termed ER-associated degradation (ERAD). Central regulators of the ERAD system are membrane-bound ubiquitin ligases, which are thought to channel misfolded proteins through the ER membrane during retrotranslocatio...

journal_title：Molecular biology of the cell

authors： Zhang T,Xu Y,Liu Y,Ye Y

更新日期：2015-12-01 00:00:00

abstract：:TPA-inducible sequence 11b/butyrate response factor 1 (TIS11b/BRF1) belongs to the tristetraprolin (TTP) family of zinc-finger proteins, which bind to mRNAs containing AU-rich elements in their 3'-untranslated region and target them for degradation. Regulation of TTP family function through phosphorylation by p38 MAP ...

journal_title：Molecular biology of the cell

authors： Rataj F,Planel S,Desroches-Castan A,Le Douce J,Lamribet K,Denis J,Feige JJ,Cherradi N

更新日期：2016-12-01 00:00:00

abstract：:The yeast vacuolar proton-translocating ATPase (V-ATPase) is a multisubunit complex comprised of peripheral membrane subunits involved in ATP hydrolysis and integral membrane subunits involved in proton pumping. The yeast vma21 mutant was isolated from a screen to identify mutants defective in V-ATPase function. vma21...

journal_title：Molecular biology of the cell

authors： Hill KJ,Stevens TH

更新日期：1994-09-01 00:00:00

abstract：:The putative role of sorting early endosomes (EEs) in synaptic-like microvesicle (SLMV) formation in the neuroendocrine PC12 cell line was investigated by quantitative immunoelectron microscopy. By BSA-gold internalization kinetics, four distinct endosomal subcompartments were distinguished: primary endocytic vesicles...

journal_title：Molecular biology of the cell

authors： de Wit H,Lichtenstein Y,Geuze HJ,Kelly RB,van der Sluijs P,Klumperman J

更新日期：1999-12-01 00:00:00

abstract：:Activation of Rap1 small GTPases stabilizes cell--cell junctions, and this activity requires Krev Interaction Trapped gene 1 (KRIT1). Loss of KRIT1 disrupts cardiovascular development and causes autosomal dominant familial cerebral cavernous malformations. Here we report that native KRIT1 protein binds the effector lo...

journal_title：Molecular biology of the cell

authors： Liu JJ,Stockton RA,Gingras AR,Ablooglu AJ,Han J,Bobkov AA,Ginsberg MH

更新日期：2011-07-15 00:00:00

abstract：:Autocrine motility factor receptor (AMF-R) is a cell surface receptor that is also localized to a smooth subdomain of the endoplasmic reticulum, the AMF-R tubule. By postembedding immunoelectron microscopy, AMF-R concentrates within smooth plasmalemmal vesicles or caveolae in both NIH-3T3 fibroblasts and HeLa cells. B...

journal_title：Molecular biology of the cell

authors： Benlimame N,Le PU,Nabi IR

更新日期：1998-07-01 00:00:00

abstract：:Genes occupy preferred spatial positions within interphase cell nuclei. However, positioning patterns are not an innate feature of a locus, and genes can alter their localization in response to physiological and pathological changes. Here we screen the radial positioning patterns of 40 genes in normal, hyperplasic, an...

journal_title：Molecular biology of the cell

authors： Leshner M,Devine M,Roloff GW,True LD,Misteli T,Meaburn KJ

更新日期：2016-01-15 00:00:00

abstract：:MARK2 regulates the establishment of polarity in Madin-Darby canine kidney (MDCK) cells in part through phosphorylation of serine 227 of Rab11-FIP2. We identified Eps15 as an interacting partner of phospho-S227-Rab11-FIP2 (pS227-FIP2). During recovery from low calcium, Eps15 localized to the lateral membrane before pS...

journal_title：Molecular biology of the cell

authors： Lapierre LA,Manning EH,Mitchell KM,Caldwell CM,Goldenring JR

更新日期：2017-04-15 00:00:00

abstract：:Whereas lysosome-related organelles (LRO) of specialized cells display both exocytic and endocytic features, lysosomes in nonspecialized cells can also acquire the property to fuse with the plasma membrane upon an acute rise in cytosolic calcium. Here, we characterize this unconventional secretory pathway in fibroblas...

journal_title：Molecular biology of the cell

authors： Laulagnier K,Schieber NL,Maritzen T,Haucke V,Parton RG,Gruenberg J

更新日期：2011-06-15 00:00:00

abstract：:The formin mDia2 mediates the formation of lamellipodia and filopodia during cell locomotion. The subcellular localization of activated mDia2 depends on interactions with actin filaments and the plasma membrane. We investigated the poorly understood mechanism of plasma membrane targeting of mDia2 and found that the en...

journal_title：Molecular biology of the cell

authors： Gorelik R,Yang C,Kameswaran V,Dominguez R,Svitkina T

更新日期：2011-01-15 00:00:00

abstract：:Chemoattractants induce neutrophil polarization through localized polymerization of F-actin at the leading edge. The suppression of rear and lateral protrusions is required for efficient chemotaxis and involves the temporal and spatial segregation of signaling molecules. We have previously shown that the intracellular...

journal_title：Molecular biology of the cell

authors： Nuzzi PA,Senetar MA,Huttenlocher A

更新日期：2007-03-01 00:00:00

abstract：:Myo1c is a member of the myosin superfamily that binds phosphatidylinositol-4,5-bisphosphate (PIP(2)), links the actin cytoskeleton to cellular membranes and plays roles in mechano-signal transduction and membrane trafficking. We located and characterized two distinct membrane binding sites within the regulatory and t...

journal_title：Molecular biology of the cell

authors： Hokanson DE,Laakso JM,Lin T,Sept D,Ostap EM

更新日期：2006-11-01 00:00:00

abstract：:The daily phagocytosis of shed photoreceptor outer segments by pigment epithelial cells is critical for the maintenance of the retina. In a subtractive polymerase chain reaction analysis, we found that functional differentiation of human ARPE19 retinal pigment epithelial (RPE) cells is accompanied by up-regulation of ...

journal_title：Molecular biology of the cell

authors： Law AL,Ling Q,Hajjar KA,Futter CE,Greenwood J,Adamson P,Wavre-Shapton ST,Moss SE,Hayes MJ

更新日期：2009-09-01 00:00:00

abstract：:Stem cells are classically defined by their multipotent, long-term proliferation, and self-renewal capabilities. Here, we show that increased antioxidant capacity represents an additional functional characteristic of muscle-derived stem cells (MDSCs). Seeking to understand the superior regenerative capacity of MDSCs c...

journal_title：Molecular biology of the cell

authors： Urish KL,Vella JB,Okada M,Deasy BM,Tobita K,Keller BB,Cao B,Piganelli JD,Huard J

更新日期：2009-01-01 00:00:00

abstract：:High-grade gliomas are especially difficult tumors to treat due to their invasive behavior. This has led to extensive research focusing on arresting glioma cell migration. Cell migration involves the sensing of a migratory cue, followed by polarization in the direction of the cue, and reorganization of the actin cytos...

journal_title：Molecular biology of the cell

authors： Mair DB,Ames HM,Li R

更新日期：2018-10-15 00:00:00

abstract：:With the use of the patch clamp technique, the role of cytoskeleton in the regulation of ion channels in plasma membrane of leukemic K562 cells was examined. Single-channel measurements have indicated that disruption of actin filaments with cytochalasin D (CD) resulted in a considerable increase of the activity of non...

journal_title：Molecular biology of the cell

authors： Negulyaev YA,Vedernikova EA,Maximov AV

更新日期：1996-12-01 00:00:00

abstract：:RIO proteins form a conserved family of atypical protein kinases. Humans possess three distinct RIO kinases-hRio1, hRio2, and hRio3, of which only hRio2 has been characterized with respect to its role in ribosomal biogenesis. Here we show that both hRio1 and hRio3, like hRio2, are associated with precursors of 40S rib...

journal_title：Molecular biology of the cell

authors： Widmann B,Wandrey F,Badertscher L,Wyler E,Pfannstiel J,Zemp I,Kutay U

更新日期：2012-01-01 00:00:00

abstract：:Mitochondrial decline is a hallmark of aging, and cells are equipped with many systems to regulate mitochondrial structure and function in response to stress and metabolic alterations. Here, using budding yeast, we identify a proteolytic pathway that contributes to alterations in mitochondrial structure in aged cells ...

journal_title：Molecular biology of the cell

authors： Goodrum JM,Lever AR,Coody TK,Gottschling DE,Hughes AL

更新日期：2019-08-01 00:00:00

abstract：:Ubiquitin-mediated proteolysis plays a key role in many pathways inside the cell and is particularly important in regulating cell cycle transitions. SCF (Skp1/Cul1/F-box protein) complexes are modular ubiquitin ligases whose specificity is determined by a substrate-binding F-box protein. Dia2 is a Saccharomyces cerevi...

journal_title：Molecular biology of the cell

authors： Koepp DM,Kile AC,Swaminathan S,Rodriguez-Rivera V

更新日期：2006-04-01 00:00:00

abstract：:The stem-loop binding protein (SLBP1) binds the 3' stem-loop of histone pre-mRNA and is required for efficient processing of histone transcripts in the nucleus. We examined the localization of SLBP1 in the germinal vesicle of Xenopus laevis oocytes. In spread preparations of germinal vesicle contents, an anti-SLBP1 an...

journal_title：Molecular biology of the cell

authors： Abbott J,Marzluff WF,Gall JG

更新日期：1999-02-01 00:00:00

abstract：:The endocytic and autophagic pathways are involved in the membrane trafficking of exogenous and endogenous materials to lysosomes. However, the mechanisms that regulate these pathways are largely unknown. We previously reported that Rubicon, a Beclin 1-binding protein, negatively regulates both the autophagic and endo...

journal_title：Molecular biology of the cell

authors： Tabata K,Matsunaga K,Sakane A,Sasaki T,Noda T,Yoshimori T

更新日期：2010-12-01 00:00:00