Abstract:
:Mammalian cells are involved in a range of biotechnological applications and more recently have been increasingly exploited in regenerative medicine. Critical to successful applications involving mammalian cells are their long-term storage and transport, for which cryopreservation in liquid nitrogen is the most frequently used strategy. However, cryopreservation suffers from high costs, difficulties in transport logistics and the use of undesirable additives (e.g. animal sera or DMSO). An alternative approach, proposed as low cost, low maintenance and process-compatible, is viable desiccation of mammalian cells. Several groups claim to have achieved this, but the extent of desiccation in the cell samples concerned is not always clear, in part because of difficulties in determining very low water content. Although several techniques exist that are frequently used to quantify the amount of water in samples (e.g. FTIR spectroscopy, thermogravimetric analysis (TGA), NMR spectroscopy), the complexity of sample preparation, as well as the costs and time constraints involved are disadvantageous. Here, we assess a novel, rapid and low cost technique, i.e. terahertz (THz) spectroscopy, for the quantification of water content within dehydrated mammalian cell samples.
journal_name
Curr Pharm Biotechnoljournal_title
Current pharmaceutical biotechnologyauthors
Chau DY,Dennis AR,Lin H,Zeitler JA,Tunnacliffe Adoi
10.2174/1389201017666151029105941subject
Has Abstractpub_date
2015-01-01 00:00:00pages
200-7issue
2eissn
1389-2010issn
1873-4316pii
CPB-EPUB-71416journal_volume
17pub_type
杂志文章abstract::CNS disorders are the third major problem of health in developed countries, with approximately 10% of direct costs associated with a pharmacological treatment of doubtful cost-effectiveness. There is an alarming abuse of psychotropic drugs worldwide and only 20-30% of patients with CNS disorders appropriately respond ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920112799857576
更新日期:2012-04-01 00:00:00
abstract::Many cationic peptides with antimicrobial properties have been isolated from bacteria, fungi, plants, and animals. These peptides vary in molecular size, potency and spectra of activities. This report surveyed the literature to highlight the peptides that have antifungal activity and greatest potential for development...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920111796117265
更新日期:2011-08-01 00:00:00
abstract::Silkworm pupae protein is a good source of high quality protein. The hydrolyzates of silkworm pupae protein catalyzed by neutrase, pepsin, acidic protease (Asperqiius usamii NO. 537), flavourzyme, alcalase, and trypsin with inhibitory activity on angiotensin I-converting enzyme (ACE) were identified by HPLC. The hydro...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/138920108785161578
更新日期:2008-08-01 00:00:00
abstract:BACKGROUND:Nitrate and nitrite can get into the body through the consumption of contaminated water either directly or indirectly. The accumulation of these compounds in the body, in the long run, leads to health problems, for example, digestive disorders, cancers, and even death threats in children. The aim of this rev...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201021666201203160012
更新日期:2020-12-03 00:00:00
abstract:BACKGROUND:Rheumatoid arthritis (RA) is a common connective tissue disease, characteristic of chronic and invasive synovitis in single or multiple joints and vasculitis. RA is a heterogeneous disease with unclear pathogenesis. Therefore, exploring the etiology and pathogenesis of the disease is essential for identifyin...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201021666201001142829
更新日期:2020-10-01 00:00:00
abstract::We have developed a high-pH, pH-shift refolding "Ph-Fold technology", both for academic research and industrial protein drug development applications. Using this technology, we were able to refold some "difficult-to-refold" proteins, some of which are proven important drug targets and protein drug candidates. The tech...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/138920110791111906
更新日期:2010-04-01 00:00:00
abstract::In the past decade, a range of dental products have appeared which make advertising claims around preventing mineral loss or repairing mineral loss. This review examines the requirements for effective protection of tooth structure, and the evidence around technologies now available for preventing tooth structure loss ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201017666161207163851
更新日期:2017-01-01 00:00:00
abstract::It is now widely believed that tumors originate from normal cells as a result of accumulated genetic/epigenetic changes. These alterations affect the signaling pathways at transcriptional and post-transcriptional level that drive cells into uncontrolled cell division, growth, and migration. Recent advancement of molec...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920108785915102
更新日期:2008-10-01 00:00:00
abstract::Long-term increase of blood pressure represents one of the most important risk factors triggering many cardiovascular diseases, and via counter-regulatory mechanisms it is itself modulated by them. Adequate perfusion of the respective areas with nutrients requires appropriate production of vasodilatory and vasoconstri...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920111798281072
更新日期:2011-09-01 00:00:00
abstract:OBJECTIVE:In the present study, Solenostemma argel effervescent tablets were prepared from Argel methanolic extract. METHODS:The tablets were examined for their ability to impede carbon tetrachloride (CCl4)-induced lipid peroxidation in mice liver. The antioxidant activities of the enzymes; super-oxide dismutase (SOD)...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201020666190617165300
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:Connexin (Cx) proteins are the building blocks of gap junctions. Among these, Cx37 and Cx40 are expressed on vascular system and reported to have cardioprotective role. Linking polymorphisms in genes coding for Cx and coronary artery disease (CAD) risk showed conflicting results in different populations. Non...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201018666171002125432
更新日期:2017-11-10 00:00:00
abstract:BACKGROUND:With the improvements in living standards, height is getting more attention. Malnutrition is one of the main causes of children's short stature, therefore nutritional intervention in adolescence is the key to prevent short stature. The peptides from Antarctic krill (AKPs), the ideal protein model, act in bon...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1381612826666200612170316
更新日期:2020-06-12 00:00:00
abstract:BACKGROUND:Although the stability of proteins is of significance to maintain protein function for therapeutical applications, this remains a challenge. Herein, a general method of preserving protein stability and function was developed using gelatin films. METHODS:Enzymes immobilized onto films composed of gelatin and...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201020666191202145351
更新日期:2020-01-01 00:00:00
abstract::This mini-review describes how single-molecule sensitive fluorescence resonance energy transfer (FRET) and photoinduced electron transfer (PET) reactions can be successfully applied to monitor conformational dynamics in biopolymers. Single-pair FRET experiments are ideally suited to study conformational dynamics occur...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201043376896
更新日期:2004-06-01 00:00:00
abstract::Tinospora cordifolia (Giloy) is a medicinal plant used in folk and Ayurvedic medicines throughout India since ancient times. All the parts of the plant are immensely useful due to the presence of different compounds of pharmaceutical importance belonging to various groups as alkaloids, diterpenoid lactones, glycosides...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201021666200430114547
更新日期:2020-01-01 00:00:00
abstract::Positron emission tomography (PET) is a powerful and rapidly developing area of molecular imaging that is used to study and visualize human physiology by the detection of positron-emitting radiopharmaceuticals. Information about metabolism, receptor/enzyme function, and biochemical mechanisms in living tissue can be o...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920110792246564
更新日期:2010-09-01 00:00:00
abstract::Tumor cells show metabolic features distinctive from normal tissues, with characteristically enhanced aerobic glycolysis, glutaminolysis and lipid synthesis. Peroxisome proliferator activated receptor α (PPAR α) is activated by nutrients (fatty acids and their derivatives) and influences these metabolic pathways actin...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201011314030009
更新日期:2013-01-01 00:00:00
abstract::The IMiDs represent a new proprietary class of thalidomide analogues that possess greater potency and less toxicity than the parent compound. As a group, these agents share the pharmacologic property of modulating cellular response to ligand activation, the precise biologic effect of which is cell lineage and stimulan...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920106778521587
更新日期:2006-10-01 00:00:00
abstract::In order to valorize novel biotechnology innovations, there is a need to evaluate ex-ante their market potential. A case in point is biofortification, i.e. the enhancement of the micronutrient content of staple crops through conventional or genetic breeding techniques. In a recent article in Nature Biotechnology, for ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/138920112804724873
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND:L2-based Human Papillomavirus (HPV) prophylactic vaccines, containing epitopes from HPV minor capsid proteins, are under investigation as second-generation HPV vaccines. No such vaccine has passed clinical trials yet, mainly due to the low immunogenicity of peptide vaccines; so efforts are being continued. A...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201020666191114104850
更新日期:2020-01-01 00:00:00
abstract::Recent technological advances in NMR methods and instrumentation are having a significant impact in structural biology. These innovations are also impacting pharmaceutical biotechnology as it is now possible to use NMR spectroscopy to rapidly characterize a growing number of prospective protein drugs and protein drug ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201053642367
更新日期:2005-04-01 00:00:00
abstract::Carbon nanotubes are nano sized cylindrical chicken wire like structures made of carbon atoms. Carbon nanotubes have applications in electronics, energy storage, electromagnetic devices, environmental remediation and in medicine as well. The biomedical applications of carbon nanotubes can be owed to features like low ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201021999201110205615
更新日期:2020-11-10 00:00:00
abstract::Cancer, as the most serious threat to human health, poses millions of deaths in the world each year. In vitro cancer cell and biomarker detection is of great importance for early cancer diagnosis and therapy, which needs highly sensitive, selective and quantitative diagnosis platforms. Threedimensional paper-based ele...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201017666160519111702
更新日期:2016-01-01 00:00:00
abstract::Raman spectroscopy has long been used as a powerful tool for chemical composition analyses. Raman scattering light measurement has the advantage of being able to examine biomolecules in a nondestructive and non-labeling manner. However, molecular imaging of cells and tissues by using Raman microscopy had been hampered...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:
更新日期:2013-01-01 00:00:00
abstract::Active or passive immunotherapy is expected to slow or stop the pathological process of Alzheimer's disease (AD). Immunotherapy for AD has demonstrated that targeting beta-amyloid (Aβ) or tau protein with vaccines or antibodies can reduce AD pathologies. Active anti-Aβ immunization for AD includes using AN1792 and sec...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920101606150407112319
更新日期:2015-01-01 00:00:00
abstract::TGF-β isoforms (TGF-β1, -β2, and -β3) are secreted signaling ligands that stimulate the expression of protein components of the extracellular matrix, regulate the growth and differentiation of epithelial cells, modulate immune cell function, and play roles in the development of several essential organs, including the ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920111798808383
更新日期:2011-12-01 00:00:00
abstract::Beta-lactams and glycopeptides antibiotics directed against enzymes involved in bacterial cell wall synthesis have generated bacterial resistance. Search for new antibiotic molecules is widely focused on bifunctional Penicillin-Binding Proteins (PBPs), with particular emphasis on their glycosyltransferase activity. Th...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201023378436
更新日期:2002-06-01 00:00:00
abstract::In this review, the compartments of the vasculature will be discussed with respect to their potential contribution to the build-up of a differentiated and functionally meaningful vessel system. Chronic ischemia of muscle tissue results in microvessel rarification, which represents a potential therapeutic target, given...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920107779941390
更新日期:2007-02-01 00:00:00
abstract::Elaborate repair pathways counteract the deleterious effects of DNA damage by mechanisms that are understood in reasonable detail. In contrast, repair of damaged RNA has not been widely explored. This may be because aberrant RNAs are generally assumed to be degraded rather than repaired. The reason for this view is we...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920107783018363
更新日期:2007-12-01 00:00:00
abstract::The interaction between reactive oxygen species (ROS) and inflammation plays an important role in the pathogenesis of endothelial dysfunction and cardiovascular disease, cancer and other diseases. Thus, antioxidant strategies may be important in immune regulation and in limiting inflammation. Surprisingly, large clini...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:
更新日期:2012-10-01 00:00:00