Abstract:
:Mammalian cells are involved in a range of biotechnological applications and more recently have been increasingly exploited in regenerative medicine. Critical to successful applications involving mammalian cells are their long-term storage and transport, for which cryopreservation in liquid nitrogen is the most frequently used strategy. However, cryopreservation suffers from high costs, difficulties in transport logistics and the use of undesirable additives (e.g. animal sera or DMSO). An alternative approach, proposed as low cost, low maintenance and process-compatible, is viable desiccation of mammalian cells. Several groups claim to have achieved this, but the extent of desiccation in the cell samples concerned is not always clear, in part because of difficulties in determining very low water content. Although several techniques exist that are frequently used to quantify the amount of water in samples (e.g. FTIR spectroscopy, thermogravimetric analysis (TGA), NMR spectroscopy), the complexity of sample preparation, as well as the costs and time constraints involved are disadvantageous. Here, we assess a novel, rapid and low cost technique, i.e. terahertz (THz) spectroscopy, for the quantification of water content within dehydrated mammalian cell samples.
journal_name
Curr Pharm Biotechnoljournal_title
Current pharmaceutical biotechnologyauthors
Chau DY,Dennis AR,Lin H,Zeitler JA,Tunnacliffe Adoi
10.2174/1389201017666151029105941subject
Has Abstractpub_date
2015-01-01 00:00:00pages
200-7issue
2eissn
1389-2010issn
1873-4316pii
CPB-EPUB-71416journal_volume
17pub_type
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journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201013378707
更新日期:2001-06-01 00:00:00
abstract::Proliposomes are phospholipid based drug delivery systems that are finding important applications in the field of pharmaceutics. Proliposomes have been extensively studied as a potential carrier for oral delivery of drugs with poor bioavailability, but the mechanism of absorption and cellular uptake pathways has not y...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201016666150118134256
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journal_title:Current pharmaceutical biotechnology
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journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
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更新日期:2020-01-01 00:00:00
abstract::There is a growing trend of herbal medicines in India as well as other parts of the world. The use of herbal medicines alone or alongside prescription drugs for disease management is quite common now. We hereby carry out a review of dominant species of Selinum mainly S. vaginatum C.B. Clarke and S. wallichianum (DC.) ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201020666181227150829
更新日期:2018-01-01 00:00:00
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journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/138920109788922173
更新日期:2009-08-01 00:00:00
abstract:OBJECTIVE:The goals of this study were to observe how luteolin protects endothelial cells (ECs) from injury stimulated by Angiotensin II (Ang II), investigate the role of vascular endothelial dysfunction in vascular smooth muscle cell proliferation and migration in vitro and investigate its primary mechanism of action....
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201015666140113113843
更新日期:2014-01-01 00:00:00
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journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:
更新日期:2015-03-03 00:00:00
abstract::Cross-border reproductive care (CBRC) is a multifaceted phenomenon. It may involve both the movement of patients to undertake assisted reproductive treatment through technologies otherwise denied and the movement of assisted reproduction professionals, egg and sperm donors and surrogates, as well as the importing and ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201017666160118103418
更新日期:2016-01-01 00:00:00
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journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201022666210119101522
更新日期:2021-01-18 00:00:00
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journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201015666140519124909
更新日期:2014-01-01 00:00:00
abstract::We have developed a high-pH, pH-shift refolding "Ph-Fold technology", both for academic research and industrial protein drug development applications. Using this technology, we were able to refold some "difficult-to-refold" proteins, some of which are proven important drug targets and protein drug candidates. The tech...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/138920110791111906
更新日期:2010-04-01 00:00:00
abstract::Calendula officinalis of family Asteraceae, also known as marigold, has been widely used from time immemorial in Indian and Arabic cultures as an anti-inflammatory agent to treat minor skin wound and infections, burns, bee stings, sunburn and cancer. At a relatively high dose, calendula can lower blood pressure and ch...
journal_title:Current pharmaceutical biotechnology
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doi:10.2174/138920110793261971
更新日期:2010-12-01 00:00:00
abstract::Elaboration of the structure of TRPV1 and its functional relationship with channel activity is a work in progress, with much remaining to be done before the structure-function relationship of TRPV1 is comprehensively elicited. The result is that the present state of knowledge can reasonably be described as a patch-wor...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920111793937934
更新日期:2011-01-01 00:00:00
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journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201016666150907113653
更新日期:2016-01-01 00:00:00
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journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920101314151120122745
更新日期:2012-11-01 00:00:00
abstract::Beta-lactams and glycopeptides antibiotics directed against enzymes involved in bacterial cell wall synthesis have generated bacterial resistance. Search for new antibiotic molecules is widely focused on bifunctional Penicillin-Binding Proteins (PBPs), with particular emphasis on their glycosyltransferase activity. Th...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201023378436
更新日期:2002-06-01 00:00:00
abstract::Hyaluronan (HA) is a highly biocompatible biopolymer that is widely used for a variety of therapeutic purposes including surgical preparations, adhesion prevention, viscosupplementation and drug and cytokine delivery. Delivery can be accomplished effectively when HA-based carriers are synthesized in the form of hydrog...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920112800624427
更新日期:2012-06-01 00:00:00
abstract::Lactobacilli are common microorganisms in diverse vegetables and meat products and several of these are also indigenous inhabitants in the gastro-intestinal (GI) tract of humans and animals where they are believed to have health promoting effects on the host. One of the highly appreciated probiotic effects is their ab...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920109787048571
更新日期:2009-01-01 00:00:00
abstract::Bone is among the most common locations of metastasis and therefore represents an important clinical target for diagnostic follow-up in cancer patients. In the pathogenesis of bone metastases, disseminated tumor cells proliferating in bone interact with the local microenvironment stimulating or inhibiting osteoclast a...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920112799436285
更新日期:2012-03-01 00:00:00
abstract:BACKGROUND:`Herbal mixtures` containing synthetic cannabinoid receptor agonists (SCRAs) are promoted as legal alternative to marihuana and are easily available via the Internet. Keeping analytical methods for the detection of these SCRAs up-to-date is a continuous challenge for clinicians and toxicologists due to the h...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201019666180509163114
更新日期:2018-01-01 00:00:00
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journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201021666200430114547
更新日期:2020-01-01 00:00:00
abstract::Natural compounds represent a significant source for the development of novel medicines. Finding the target proteins for a natural compound is the most important step towards understanding its molecular mechanism for therapeutic usage. In fact, the search for target proteins could be considered the first step of the d...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920112800958940
更新日期:2012-07-01 00:00:00
abstract::Mesenchymal stem cells (MSCs), a form of adult stem cells, are known to have a self-renewing property and the potential to specialize into a multitude of cells and tissues such as adipocytes, cartilage cells, and fibroblasts. MSCs can migrate and home to the desired target zone where inflammation is present. The uniqu...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201021666200907121530
更新日期:2020-09-07 00:00:00
abstract:BACKGROUND:Terminalia arjuna (TA) has been reported and explored traditionally for its cardiotonic properties while the mechanism of antihypertensive effect of TA has not been clearly reported. METHOD:The oxidative stress is a major cause for hypertension, hence different extracts of TA having variable marker yield we...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201020666190222185209
更新日期:2019-01-01 00:00:00
abstract::The prospect of mobilizing the power of the immune system in the fight against cancer has fascinated generations of immunologists. After decades of sporadic successes scattered amongst frequent failures, the field of tumor immunology is now poised to take advantage of a greatly improved understanding of the molecular ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920110792246528
更新日期:2010-09-01 00:00:00
abstract::In order to valorize novel biotechnology innovations, there is a need to evaluate ex-ante their market potential. A case in point is biofortification, i.e. the enhancement of the micronutrient content of staple crops through conventional or genetic breeding techniques. In a recent article in Nature Biotechnology, for ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/138920112804724873
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND:Oxymatrine is one of the most promising alkaloids from Sophora flavescens for its excellent pharmacological effects. OBJECTIVE:The aim of this research is to assess the biopharmaceutical and pharmacokinetic activities of oxymatrine, and clarify its mechanisms of absorption and metabolism. METHODS:The biolo...
journal_title:Current pharmaceutical biotechnology
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doi:10.2174/1389201022666210118160529
更新日期:2021-01-18 00:00:00
abstract::This review describes microbial transformation studies of natural drugs, its importance in correlating them with the corresponding metabolism (biotransformation) in animal system and in the structural modification of complex drug molecules, which are difficult to obtain synthetically. Emphasis is laid on the potential...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201033489847
更新日期:2003-06-01 00:00:00
abstract::Several of the 28 mammalian transient receptor potential (TRP) channel subunits are expressed throughout the alimentary canal where they play important roles in taste, chemo- and mechanosensation, thermoregulation, pain and hyperalgesia, mucosal function and homeostasis, control of motility by neurons, interstitial ce...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920111793937862
更新日期:2011-01-01 00:00:00