Abstract:
:Recent technological advances in NMR methods and instrumentation are having a significant impact in structural biology. These innovations are also impacting pharmaceutical biotechnology as it is now possible to use NMR spectroscopy to rapidly characterize a growing number of prospective protein drugs and protein drug targets. This review provides a general summary of how solution-state NMR can be used to determine protein structures. It also focuses on exploring how advances in solution state NMR are changing the way in which protein structures can be determined and protein-ligand interactions can be characterized. Recent innovations in protein sample preparation, in instrumentation and data collection, in spectral assignment and in structure generation are highlighted. The impact of solution-state NMR on pharmaceutical biotechnology is also discussed, with a special emphasis on describing how NMR has been used to study a number of pharmaceutically important proteins and how NMR is currently being used to rapidly screen and to map the binding sites of small molecules to a range of protein targets.
journal_name
Curr Pharm Biotechnoljournal_title
Current pharmaceutical biotechnologyauthors
Wishart Ddoi
10.2174/1389201053642367keywords:
subject
Has Abstractpub_date
2005-04-01 00:00:00pages
105-20issue
2eissn
1389-2010issn
1873-4316journal_volume
6pub_type
杂志文章,评审abstract:BACKGROUND:In this study, local sustainable enzyme sources involving excised digestive juice of African land snail and yeast were utilized to achieve the simultaneous saccharification (SSF) and fermentation (SSF) of plantain pseudostem (PPS) waste, and afterwards their effects on terpenoids using gas chromatography cou...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201020666190408120018
更新日期:2019-01-01 00:00:00
abstract:AIM OF THE STUDY:To assess tramadol and O-desmethyltramadol (M1) concentrations and their correlations with analgesia in patients with cancer pain. PATIENTS AND METHODS:Thirty opioid-naive patients with nociceptive pain intensity on VAS (visual analogue scale) > 40 received controlled-release tramadol as the first (15...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/138920111794295738
更新日期:2011-02-01 00:00:00
abstract::Lipid nanocapsules with their unique composition represent a promising biocompatible drug delivery platform in nanometer range with narrow size distribution. They are highly stable in comparison to other nano-vectors and were found to impart very desirable characteristics to the therapeutic molecule being delivered wi...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920101604150218103555
更新日期:2015-01-01 00:00:00
abstract::CNS disorders are the third major problem of health in developed countries, with approximately 10% of direct costs associated with a pharmacological treatment of doubtful cost-effectiveness. There is an alarming abuse of psychotropic drugs worldwide and only 20-30% of patients with CNS disorders appropriately respond ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920112799857576
更新日期:2012-04-01 00:00:00
abstract:BACKGROUND:Enterotoxigenic Escherichia coli (ETEC) is the main cause of fatal diarrhea in piglets during the first week of life and over the time of weaning. Pathogenesis of ETEC-causing diarrhea involves intestinal colonization mediated by fimbriae. Although, both IgY and egg yolk phosvitin (PV) possess antimicrobial ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201018666170425120036
更新日期:2017-01-01 00:00:00
abstract::The triterpenes α- and β-amyrins display important pharmacological activities. As a result of their high hydrophobia, they present low water solubility and reflect poor oral bioavailability. Different techniques can be used for the improvement of amyrins solubility, one of them is the use of nanoemulsions. Therefore, ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201015666140317121647
更新日期:2013-01-01 00:00:00
abstract::Tinospora cordifolia (Giloy) is a medicinal plant used in folk and Ayurvedic medicines throughout India since ancient times. All the parts of the plant are immensely useful due to the presence of different compounds of pharmaceutical importance belonging to various groups as alkaloids, diterpenoid lactones, glycosides...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201021666200430114547
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Osteosarcoma is a malignant tumor type that starts in bone and occurs most frequently in adolescents. Traditional techniques are insufficient, especially for metastatic ones. As alternative treatment techniques, natural products are worthy to examine due to being safe and innovative. Essential polyunsaturate...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201021666200928101029
更新日期:2020-09-27 00:00:00
abstract:BACKGROUND:In Saudi Arabia, the incidence and mortality rates of breast cancer are high. Although current treatments are effective, breast cancer cells develop resistance to these treatments. Numerous studies have demonstrated that active compounds in plant extracts, such as the phenolic compound Rosmarinic Acid (RA), ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201021666200318114817
更新日期:2020-01-01 00:00:00
abstract::Using gene knockout mice of particular genes is one of the most effective methods in conducting successful study on the mode of action of target gene products in targeted organs. So called the knockout technology is now a powerful tool that can lead us to find clear understanding on difficult questions such as the eff...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/138920109787314999
更新日期:2009-02-01 00:00:00
abstract::The growing prevalence of disorders of lipid and sterol homeostasis such as obesity and atherosclerosis in Western societies has increased demand for the development of therapies for their prevention and treatment. Crucial for this development is an understanding of the underlying cellular mechanisms which are involve...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920112799095329
更新日期:2012-02-01 00:00:00
abstract::The interaction between reactive oxygen species (ROS) and inflammation plays an important role in the pathogenesis of endothelial dysfunction and cardiovascular disease, cancer and other diseases. Thus, antioxidant strategies may be important in immune regulation and in limiting inflammation. Surprisingly, large clini...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:
更新日期:2012-10-01 00:00:00
abstract::Hepatic metallothionein (MT) expression, with various isoforms, and varying cellular localizations is a useful marker for clinico-pathogenesis of liver diseases. In acute liver toxicity caused by cadmium, carbon tetrachloride, or acetaminophen, MT plays a protective role, via the scavenging of radical species. In chro...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201011314040006
更新日期:2013-01-01 00:00:00
abstract::Natural compounds represent a significant source for the development of novel medicines. Finding the target proteins for a natural compound is the most important step towards understanding its molecular mechanism for therapeutic usage. In fact, the search for target proteins could be considered the first step of the d...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920112800958940
更新日期:2012-07-01 00:00:00
abstract:BACKGROUND:Rheumatoid arthritis (RA) is a common connective tissue disease, characteristic of chronic and invasive synovitis in single or multiple joints and vasculitis. RA is a heterogeneous disease with unclear pathogenesis. Therefore, exploring the etiology and pathogenesis of the disease is essential for identifyin...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201021666201001142829
更新日期:2020-10-01 00:00:00
abstract::Coagulase-negative staphylococci (CoNS) are part of the microbiota of human skin and rarely linked with soft tissue infections. In recent years, CoNS species considered as one of the major nosocomial pathogens and can cause several infections such as catheter-acquired sepsis, skin infection, urinary tract infection, e...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201021666200423110359
更新日期:2020-01-01 00:00:00
abstract::The non-Hodgkin's lymphomas are a diverse groups of lymphoid neoplasms that collectively rank fifth in cancer incidence and mortality. Conventional treatment for patients with newly-diagnosed non-Hodgkin's lymphoma (NHL) includes radiation or chemotherapy. In addition, those with asymptomatic low-grade disease may fol...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201013378581
更新日期:2001-12-01 00:00:00
abstract::Signals relayed through G protein-coupled receptors (GPCR) play pivotal roles in human physiology and are important drug targets. About 40% of all GPCRs couple to the heterotrimeric G protein Gq. Biochemical studies as well as crystallography have improved our understanding of GqPCRs and their downstream partners. Her...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201015666140922101637
更新日期:2014-01-01 00:00:00
abstract::Discovery of disease specific biomarkers in human body fluids has become an important challenge in clinical proteomics. Facing the increasing threat of degenerative and disabling diseases like cancer, cardiovascular, neurological and inflammatory diseases in large parts of the world's population, there is an urgent ne...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920106777549713
更新日期:2006-06-01 00:00:00
abstract::Nitric oxide (NO) participates in the control of the cardiovascular system where two constitutive isoforms of NO-synthase were discovered: endothelial and neuronal. Both isoforms were observed in various cells, however, endothelial NO-synthase is predominantly present in the endothelium. Injury of the endothelium dist...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920111798280992
更新日期:2011-09-01 00:00:00
abstract::Long-term increase of blood pressure represents one of the most important risk factors triggering many cardiovascular diseases, and via counter-regulatory mechanisms it is itself modulated by them. Adequate perfusion of the respective areas with nutrients requires appropriate production of vasodilatory and vasoconstri...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920111798281072
更新日期:2011-09-01 00:00:00
abstract:BACKGROUND:Up to 25% of diabetic patients will develop a diabetic foot ulcer. Chronic wounds such as diabetic foot ulcers often fail to heal with conventional therapies. In recent years, it has been identified that chronic wounds are usually associated with elevated level of matrix metalloproteinases (MMPs). Doxycyclin...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201018666170519095339
更新日期:2017-01-01 00:00:00
abstract::Midkine is a heparin-binding growth factor that promotes cell growth, survival, and migration. Externally added midkine prevents ventricular remodeling and improves long-term survival after myocardial infarction in the mouse. Preclinical testing of this protein is in progress. Externally added pleiotrophin, a member o...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201014666131226114919
更新日期:2013-01-01 00:00:00
abstract::In this review, the compartments of the vasculature will be discussed with respect to their potential contribution to the build-up of a differentiated and functionally meaningful vessel system. Chronic ischemia of muscle tissue results in microvessel rarification, which represents a potential therapeutic target, given...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920107779941390
更新日期:2007-02-01 00:00:00
abstract:BACKGROUND:Large-scale population studies showed that the SNP rs1764391 of Connexin37 gene also known as Cx37 gene may play a pivotal role in the occurrence and development of acute myocardial infarction (AMI). Published results, however, are highly controversial. OBJECTIVE:This study aimed to examine the association ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201019666191003150015
更新日期:2020-01-01 00:00:00
abstract::TGF-β isoforms (TGF-β1, -β2, and -β3) are secreted signaling ligands that stimulate the expression of protein components of the extracellular matrix, regulate the growth and differentiation of epithelial cells, modulate immune cell function, and play roles in the development of several essential organs, including the ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920111798808383
更新日期:2011-12-01 00:00:00
abstract::Malignant glioma is a primary tumor of the central nervous system, representing a major cause of mortality in a young, productive subset of population. The management of this neoplasm requires aggressive treatments, including radiotherapy. Accurate imaging plays a central role in treatment planning process with curati...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201015666140408122318
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Sexual dysfunction and infertility are conditions with high prevalence in the general population. Nutritional factors have been reported to have an impact on sexual and reproductive health. OBJECTIVE:The aim of this review is to summarize the data on nutritional factors that have influence on male and femal...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201019666180925140400
更新日期:2019-01-01 00:00:00
abstract::The biliary secretion of bile acids is critical for multiple liver functions including digesting fatty nutrients and driving bile flow. When this process is impaired, the accumulating bile acids cause inflammatory liver injury. Multiple ABC transporters in the liver are key players to safeguard the hepatocyte and avoi...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920111795163986
更新日期:2011-04-01 00:00:00
abstract::Objective: CME-1 is a polysaccharide purified from the mycelia of medicinal mushroom Cordyceps sinensis, its molecular weight was determined to be 27.6 kDa by using nuclear magnetic resonance and gas chromatography-mass spectrometry. The initiation of arterial thromboses is relevant to various cardiovascular diseases ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:
更新日期:2015-03-03 00:00:00