Abstract:
BACKGROUND:Osteosarcoma is a malignant tumor type that starts in bone and occurs most frequently in adolescents. Traditional techniques are insufficient, especially for metastatic ones. As alternative treatment techniques, natural products are worthy to examine due to being safe and innovative. Essential polyunsaturated fatty acids, mainly omega-3 and omega-6 fatty acids, have various positive effects on human health and growth. In addition, some PUFAs show anticancer activity by inducing apoptosis specifically in cancer cells. OBJECTIVE:Here, the study aims to investigate the time and dose-dependent effects of oil extract from Schizochytrium sp. against osteosarcoma cell line. MATERIALS AND METHODS:Human fetal osteoblast cells (hFOB) and osteosarcoma cells (SAOS-2) were treated with different concentrations of fatty acid samples. GC-FID was performed for fatty acid composition analysis of Schizochytrium sp. MTT-cell viability and Annexin V-apoptosis assays were performed to investigate the time and dose-dependent effects of the samples on cell lines. RESULTS:Oil extract sample has specific activity against SAOS-2 cancer line and decreases cell proliferation especially at high dose treatments. Apoptosis assay results indicate that the oil extract sample causes a significant increase in the number of apoptotic cells in SAOS-2 cell line (71.7% of SAOS-2 cells), which shows its selective activity against bone cancer cell line as a natural anti-cancer molecule. CONCLUSION:It was observed that Schizochytrium sp. extract has a time and dose-dependent ability to induce apoptosis specifically in SAOS-2 cells.
journal_name
Curr Pharm Biotechnoljournal_title
Current pharmaceutical biotechnologyauthors
Sahin Ddoi
10.2174/1389201021666200928101029subject
Has Abstractpub_date
2020-09-27 00:00:00eissn
1389-2010issn
1873-4316pii
CPB-EPUB-110269pub_type
杂志文章abstract::Silkworm pupae protein is a good source of high quality protein. The hydrolyzates of silkworm pupae protein catalyzed by neutrase, pepsin, acidic protease (Asperqiius usamii NO. 537), flavourzyme, alcalase, and trypsin with inhibitory activity on angiotensin I-converting enzyme (ACE) were identified by HPLC. The hydro...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/138920108785161578
更新日期:2008-08-01 00:00:00
abstract:BACKGROUND & OBJECTIVE:Pseudomonas aeruginosa shows resistance to a large number of antibiotics, including carbapenems and third generation cephalosporin. According to the World Health Organization global report published in February 2017, Pseudomonas aeruginosa is on the priority list among resistant bacteria, for whi...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201020666190719123133
更新日期:2019-01-01 00:00:00
abstract::Mammalian cells are involved in a range of biotechnological applications and more recently have been increasingly exploited in regenerative medicine. Critical to successful applications involving mammalian cells are their long-term storage and transport, for which cryopreservation in liquid nitrogen is the most freque...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201017666151029105941
更新日期:2015-01-01 00:00:00
abstract::The ubiquitin-proteasome pathway (UPP) is the major non-lysosomal proteolytic system in the cytosol and nucleus of all eukaryotic cells. Bortezomib (also known as PS-341 and Velcade) is a proteasome inhibitor, a novel class of cancer therapies. Bortezomib blocks multi-ubiquitinated protein degradation by inhibiting 26...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920106779116865
更新日期:2006-12-01 00:00:00
abstract::In this review, the compartments of the vasculature will be discussed with respect to their potential contribution to the build-up of a differentiated and functionally meaningful vessel system. Chronic ischemia of muscle tissue results in microvessel rarification, which represents a potential therapeutic target, given...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920107779941390
更新日期:2007-02-01 00:00:00
abstract::Raman spectroscopy has long been used as a powerful tool for chemical composition analyses. Raman scattering light measurement has the advantage of being able to examine biomolecules in a nondestructive and non-labeling manner. However, molecular imaging of cells and tissues by using Raman microscopy had been hampered...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:
更新日期:2013-01-01 00:00:00
abstract:AIMS:Candida species is the common cause of opportunistic fungal infections all over the world with increased mortality and morbidity especially in immunosuppressed patients. Fluconazole is the first line therapy for candidiasis. The antifungal resistance pattern in high-risk patients is a major concern. The present st...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201019666180515104800
更新日期:2018-01-01 00:00:00
abstract::Carbon monoxide (CO) has long been considered a purely toxic by-product of incomplete combustion processes. Acute exposure to high concentrations of CO is one of the leading causes of fatal poisoning in industrial countries. However, after two decades of intensive research, there is ample evidence that CO endogenously...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920112800399103
更新日期:2012-05-01 00:00:00
abstract::Beyond their originally sole mechanical function, current drug-eluting stents (DES) implement the concept of local drug delivery for the re-opening of stenotic arterial vessels, and for prevention of in-stent restenosis as one of the major limitations of conventional bare metal stents (BMS). Current DES consist of a p...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:In Saudi Arabia, the incidence and mortality rates of breast cancer are high. Although current treatments are effective, breast cancer cells develop resistance to these treatments. Numerous studies have demonstrated that active compounds in plant extracts, such as the phenolic compound Rosmarinic Acid (RA), ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201021666200318114817
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Nitrate and nitrite can get into the body through the consumption of contaminated water either directly or indirectly. The accumulation of these compounds in the body, in the long run, leads to health problems, for example, digestive disorders, cancers, and even death threats in children. The aim of this rev...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201021666201203160012
更新日期:2020-12-03 00:00:00
abstract:OBJECTIVE:In the present study, Solenostemma argel effervescent tablets were prepared from Argel methanolic extract. METHODS:The tablets were examined for their ability to impede carbon tetrachloride (CCl4)-induced lipid peroxidation in mice liver. The antioxidant activities of the enzymes; super-oxide dismutase (SOD)...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201020666190617165300
更新日期:2019-01-01 00:00:00
abstract::One of the most surprising aspects of neural development is that cells do not remain in their birthplace but actively migrate along a variety of routes to their final destinations. This review traces past, present, and future techniques used to analyze progenitor cell migration in the brain, and also discusses their r...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920107780906513
更新日期:2007-06-01 00:00:00
abstract::In many signaling pathways, various factors have been isolated by molecular genetic studies and wholegenome analysis. Understanding the interactions among these factors is crucial to understanding the signaling process as a whole. Recent studies on auxin signaling in the regulation of plant growth and development have...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920101314151120122745
更新日期:2012-11-01 00:00:00
abstract::The idea of cancer stem cell (CSC) has recently moved to the forefront of cancer research. There is still a lack of a widespread consensus on their description and definition. ,The increasing literature on CSCs has compelled researchers worldwide to rewrite the natural history of cancer including those cells as princi...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920111794295756
更新日期:2011-02-01 00:00:00
abstract::Lipid nanocapsules with their unique composition represent a promising biocompatible drug delivery platform in nanometer range with narrow size distribution. They are highly stable in comparison to other nano-vectors and were found to impart very desirable characteristics to the therapeutic molecule being delivered wi...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920101604150218103555
更新日期:2015-01-01 00:00:00
abstract::Bone grafts are widely used for augmentation procedures in oral and maxillofacial surgery, with autogenous bone being the gold standard. Recently, the focus of research has shifted towards synthetic bone substitutes, as no second surgery is needed and large quantities of graft can easily be provided. Within the broad ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201017666161202103218
更新日期:2017-01-01 00:00:00
abstract::Positron emission tomography (PET) is a powerful and rapidly developing area of molecular imaging that is used to study and visualize human physiology by the detection of positron-emitting radiopharmaceuticals. Information about metabolism, receptor/enzyme function, and biochemical mechanisms in living tissue can be o...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920110792246564
更新日期:2010-09-01 00:00:00
abstract:PURPOSE:Advanced gastrointestinal stromal tumours (GISTs) are treated with tyrosine kinase inhibitors, which also have antiangiogenic properties. Dual-energy CT (DECT) allows to acquire semi-quantitative iodine images which might correlate with blood pool and tumor vascularity. In this feasibility-study, we correlated ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/138920111795164066
更新日期:2011-04-01 00:00:00
abstract::Proliposomes are phospholipid based drug delivery systems that are finding important applications in the field of pharmaceutics. Proliposomes have been extensively studied as a potential carrier for oral delivery of drugs with poor bioavailability, but the mechanism of absorption and cellular uptake pathways has not y...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201016666150118134256
更新日期:2015-01-01 00:00:00
abstract::Cancer is one of the leading causes of death in the United States and accounts for approximately 8 million deaths per year worldwide. Although there is an increasing number of therapeutic options available for patients with cancer, their efficacy is time-limited and non-curative. Approximately 50-60% cancer patients i...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920112798868566
更新日期:2012-01-01 00:00:00
abstract::Coagulase-negative staphylococci (CoNS) are part of the microbiota of human skin and rarely linked with soft tissue infections. In recent years, CoNS species considered as one of the major nosocomial pathogens and can cause several infections such as catheter-acquired sepsis, skin infection, urinary tract infection, e...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201021666200423110359
更新日期:2020-01-01 00:00:00
abstract::Acute myeloid leukemia (AML) is characterized as a heterogeneous disease where the patients are sub grouped according to several classification systems and mutational analyses. Diagnosis of AML is based on identification of the specific myeloid cell initiating the disease, quantification of immature blasts in bone mar...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201016666150907113653
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Carbon nanotubes (CNTs) have been considered highly successful and proficient in terms of their mechanical, thermal and electrical functionalization and biocompatibility. In regards to their significant extent in bone regeneration, it has been determined that CNTs hold the capability to endure clinical appli...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章
doi:10.2174/1389201018666170127105555
更新日期:2017-01-01 00:00:00
abstract::Tinospora cordifolia (Giloy) is a medicinal plant used in folk and Ayurvedic medicines throughout India since ancient times. All the parts of the plant are immensely useful due to the presence of different compounds of pharmaceutical importance belonging to various groups as alkaloids, diterpenoid lactones, glycosides...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201021666200430114547
更新日期:2020-01-01 00:00:00
abstract::This review describes microbial transformation studies of natural drugs, its importance in correlating them with the corresponding metabolism (biotransformation) in animal system and in the structural modification of complex drug molecules, which are difficult to obtain synthetically. Emphasis is laid on the potential...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201033489847
更新日期:2003-06-01 00:00:00
abstract::The prospect of mobilizing the power of the immune system in the fight against cancer has fascinated generations of immunologists. After decades of sporadic successes scattered amongst frequent failures, the field of tumor immunology is now poised to take advantage of a greatly improved understanding of the molecular ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920110792246528
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND:The pregnancy pathology preeclampsia is still among the leading causes of maternal and perinatal morbidity and mortality. At the same time, its etiology is far from being identified and remains obscure in a number of facets. A number of hypotheses have been developed to explain the altered interplay between ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201019666180427110547
更新日期:2018-01-01 00:00:00
abstract::Gene targeting can be loosely defined as a process through which a specific chromosomal sequence is recognized and bound by a reagent designed for the purpose. The endpoint may be modulation of events at the target, such as transcription, or a permanent change in sequence at the site. A facile strategy for mammalian c...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201043376625
更新日期:2004-10-01 00:00:00
abstract::Whole-cell bio-chips for functional sensing integrate living cells on miniaturized platforms made by micro-system-technologies (MST). The cells are integrated, deposited or immersed in a media which is in contact with the chip. The cells behavior is monitored via electrical, electrochemical or optical methods. In this...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/138920110791233325
更新日期:2010-06-01 00:00:00