Abstract：
:Roquins are a family of highly conserved RNA-binding proteins that also contain a RING-type E3 ubiquitin ligase domain. They repress constitutive decay elements containing mRNAs and play a critical role in RNA homeostasis and immunological self-tolerance. Here we present the crystal structures of the RNA-binding region of Roquin paralog RC3H2 in both apo- and RNA-bound forms. The RNA-binding region has a bipartite architecture composed of ROQ and HEPN domains, and can bind to stem-loop and double-stranded RNAs simultaneously. The two domains undergo a large orientation change to accommodate RNA duplex binding. We profiled E2 ubiquitin-conjugating enzymes that pair with Roquins and found that RC3H1 and RC3H2 interact with two sets of overlapping but not identical E2 enzymes to drive the assembly of polyubiquitin chains of different linkages. Crystal structures, small-angle X-ray scattering, and E2 profiling revealed that while the two paralogs are highly homologous, RC3H2 and RC3H1 are different in their structures and functions. We also demonstrated that RNA duplex binding to RC3H2 cross-talks with its E3 ubiquitin ligase function using an in vitro auto-ubiquitination assay.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Zhang Q,Fan L,Hou F,Dong A,Wang YX,Tong Ydoi
10.1038/srep15660subject
Has Abstractpub_date
2015-10-22 00:00:00pages
15660issn
2045-2322pii
srep15660journal_volume
5pub_type
杂志文章abstract：:Mediterranean Sea ecosystems are considered as hotspots of biological introductions, exposed to possible negative effects of non-indigenous species. In such temperate marine ecosystems, macroalgae may be dominant, with a great percentage of their diversity represented by introduced species. Their interaction with temp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42625
更新日期：2017-02-20 00:00:00
abstract：:Factor induced reprogramming of fibroblasts is an orchestrated but inefficient process. At the epigenetic level, it results in drastic chromatin changes to erase the existing somatic "memory" and to establish the pluripotent state. Accordingly, alterations of chromatin regulators including Ezh2 influence iPSC generati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08229
更新日期：2015-02-04 00:00:00
abstract：:In this article a new spent catalyst sample preparation method was implemented for the purpose of molybdenum leaching in a single step. Further molybdenum and vanadium in the leach liquor were separated and their concentrations were enriched using the solvent extraction and stripping techniques. The impervious sulfur ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-58972-x
更新日期：2020-02-06 00:00:00
abstract：:Blood coagulation is regulated through protein-protein and protein-lipid interactions that occur at the sub-endothelium following vascular damage. Soluble clotting proteins bind to membrane components in a phosphatidylserine (PS) dependent manner to assemble multi-protein complexes that regulate clot formation; howeve...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73647-3
更新日期：2020-10-15 00:00:00
abstract：:Due to the different craniocervical structures in humans and cetaceans, a standardised method assessing the normal craniocervical relationship in cetaceans is lacking, causing difficulties in defining the presence of atlanto-occipital dissociation (AOD) in cetaceans. The present study aimed to 1) describe a novel stan...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26866-8
更新日期：2018-05-31 00:00:00
abstract：:Circulating levels of Brain Derived Neurotrophic Factor (BDNF) are lower in coronary heart disease (CHD) than in healthy subjects and are associated with coronary events and mortality. However, the mechanism(s) underling this association is not fully understood. We hypothesize that BDNF may influence fibrin fiber stru...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/s41598-018-37117-1
更新日期：2019-01-23 00:00:00
abstract：:The analysis of structural and functional neuroimaging data using graph theory has increasingly become a popular approach for visualising and understanding anatomical and functional relationships between different cerebral areas. In this work we applied a network-based approach for brain PET studies using population-b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39005-8
更新日期：2019-02-21 00:00:00
abstract：:Streptomyces coelicolor is a Gram-positive microorganism often used as a model of physiological and morphological differentiation in streptomycetes, prolific producers of secondary metabolites with important biological activities. In the present study, we analysed Streptomyces coelicolor growth and differentiation in ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32027-8
更新日期：2018-09-12 00:00:00
abstract：:Spermatogenesis is a highly regulated process during which haploid sperm cells are generated. Although autophagy is involved in the spermatogenesis process, the molecular pathways and regulations of autophagy in germ cell development remain elusive. Here, we showed that Ppp1r36, a regulatory subunit of protein phospha...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24609
更新日期：2016-04-21 00:00:00
abstract：:The spinal dorsal horn (SDH) is comprised of distinct neuronal populations that process different somatosensory modalities. Somatostatin (SST)-expressing interneurons in the SDH have been implicated specifically in mediating mechanical pain. Identifying the transcriptomic profile of SST neurons could elucidate the uni...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-25110-7
更新日期：2018-05-01 00:00:00
abstract：:Due to distinct biological behavior of mucinous adenocarcinoma (MAC) and signet ring cell cancer (SRC), the efficacy of radiotherapy on long-term outcome for rectal cancer (RC) patients with mucinous tumors is still unclear. Here, we identified 1808 RC patients with MAC/SRC from Surveillance, Epidemiology, and End-Res...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43821
更新日期：2017-03-08 00:00:00
abstract：:Crystal and electronic structures of ~380 nm BiFeO3 film grown on LaAlO3 substrate are comprehensively studied using advanced transmission electron microscopy (TEM) technique combined with first-principles theory. Cross-sectional TEM images reveal the BiFeO3 film consists of two zones with different crystal structures...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46498
更新日期：2017-04-19 00:00:00
abstract：:Neural networks can efficiently encode the probability distribution of errors in an error correcting code. Moreover, these distributions can be conditioned on the syndromes of the corresponding errors. This paves a path forward for a decoder that employs a neural network to calculate the conditional distribution, then...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11266-1
更新日期：2017-09-08 00:00:00
abstract：:One mechanism of action for the anticaries effect of topical fluoridation is through precipitation of CaF2. In this in vitro study energy-dispersive x-ray spectroscopy (EDX) is used as a semiquantitative method to detect enamel fluoride-precipitation under the influence of acidic and neutral pH-value and absence or pr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49742-5
更新日期：2019-09-17 00:00:00
abstract：:Hepatocellular carcinoma is the third leading cause of cancer-related deaths worldwide. Many patients are not eligible for curative therapies, such as surgical resection of the tumor or a liver transplant. Transarterial embolization is one therapy clinically used in these cases; however, this requires a long procedure...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47309-y
更新日期：2019-07-30 00:00:00
abstract：:Human Influenza A virus undergoes recurrent changes in the hemagglutinin (HA) surface protein, primarily involved in the human antibody recognition. Relevant antigenic changes, enabling the virus to evade host immune response, have been recognized to occur in parallel to multiple mutations at antigenic sites in HA. Ye...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02705
更新日期：2013-01-01 00:00:00
abstract：:Glioblastoma multiforme (GBM) is a highly invasive and chemoradioresistant brain malignancy. Temozolomide (TMZ), a DNA-alkylating agent, is effective against GBM and has become the standard first-line drug. However, the mechanism by which TMZ regulates the progression of GBM remains elusive. Here, we demonstrate that ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01160
更新日期：2013-01-01 00:00:00
abstract：:The roles of CD4 + T cells and CD8 + T cells in hepatitis B virus (HBV) infection have been well documented. However, the role of innate immunity in HBV infection remains obscure. Here we examined the effect of activation of innate immunity by polyinosinic: polycytidylic acid (PolyI:C) on HBV infection. A chronic HBV ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00387-2
更新日期：2017-03-22 00:00:00
abstract：:The Ganges Brahmaputra Meghna Delta (GBMD) is a large and complex coastal system whose channel network is vulnerable to morphological changes caused by sea level rise, subsidence, anthropogenic modifications, and changes to water and sediment loads. Locating and characterizing change is particularly challenging becaus...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69688-3
更新日期：2020-07-30 00:00:00
abstract：:In this work, TiN film deposited by plasma enhanced atomic layer deposition (PEALD) is adopted to modify the commercial anatase TiO2 powders. A series of analyses indicate that the surface modification of 20, 50 and 100 cycles of TiN by PEALD does not change the morphology, crystal size, lattice parameters, and surfac...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30726-w
更新日期：2018-08-14 00:00:00
abstract：:We report that misfolded cytosolic proteins can be cleared from mammalian cells by directing them to endoplasmic reticulum (ER). NAT1 R64W and Parkin R42P are naturally occurring misfolded variants of cytosolic enzymes that acetylate arylamines and ubiquitinate proteins, respectively. We demonstrate that proteasome in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12088
更新日期：2015-07-14 00:00:00
abstract：:In the central nervous system of vertebrates, cell bodies of neurons are often assembled as nuclei or cellular layers that play specific roles as functional units. The purpose of this work was to selectively highlight such cell assemblies by magnetic resonance imaging using signals from water protons that are associat...
journal_title：Scientific reports
pub_type： 杂志文章,评审
doi：10.1038/s41598-019-41587-2
更新日期：2019-03-25 00:00:00
abstract：:When compared to surface ecosystems, groundwater sampling has unique constraints, including limited access to ecosystems through wells. In order to monitor groundwater, a detailed understanding of groundwater biota and what biological sampling of wells truly reflects, is paramount. This study aims to address this unce...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40702
更新日期：2017-01-19 00:00:00
abstract：:Despite recent advances in cancer management and therapy, resistance to cytotoxic medications remains a major clinical challenge; hence, combination-based anti-cancer treatment regimens are currently gaining momentum. PTC-209 reduced BMI1 protein expression, while palbociclib inhibited CDK4, Rb, and pRbSer795 protein ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50140-0
更新日期：2019-09-23 00:00:00
abstract：:Stroke incidence is a multifactorial disease and especially hyperhomocysteinemia is associated with a higher risk of stroke. Previous studies have reported a folate metabolism disorder associated with the MTHFR gene. We investigated four single nucleotide polymorphisms in the MTHFR 3'-UTR [2572 C > A (rs4846049), 4869...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12668-x
更新日期：2017-09-29 00:00:00
abstract：:Human pancreatic islet amyloid polypeptide (hIAPP) and beta amyloid (Aβ) can accumulate in Type 2 diabetes (T2D) and Alzheimer's disease (AD) brains and evidence suggests that interaction between the two amyloidogenic proteins can lead to the formation of heterocomplex aggregates. However, the structure and consequenc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66602-9
更新日期：2020-06-25 00:00:00
abstract：:Hydrogen in the Earth's deep interior has been thought to exist as a hydroxyl group in high-pressure minerals. We present Muon Spin Rotation experiments on SiO2 stishovite, which is an archetypal high-pressure mineral. Positive muon (which can be considered as a light isotope of proton) implanted in stishovite was fou...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08437
更新日期：2015-02-13 00:00:00
abstract：:A central goal of electronics based on correlated materials or 'Mottronics' is the ability to switch between distinct collective states with a control voltage. Small changes in structure and charge density near a transition can tip the balance between competing phases, leading to dramatic changes in electronic and mag...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01876
更新日期：2013-01-01 00:00:00
abstract：:Climate change is expected to have profound, partly unforeseeable effects on the composition of functional traits of complex ecosystems, such as coral reefs, and some ecosystem properties are at risk of disappearing. This study applies a novel spatially explicit, individual-based model to explore three critical life h...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-38962-4
更新日期：2019-03-06 00:00:00
abstract：:The function of Forkhead box O 1 (FOXO1) and pSerine256-FOXO1 immunostaining in esophageal cancer is unclear. To clarify the prognostic role of nuclear FOXO1 and cytoplasmic pSerine256-FOXO1 immunostaining, a tissue microarray containing more than 600 esophageal cancers was analyzed. In non-neoplastic esophageal mucos...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35459-4
更新日期：2018-11-26 00:00:00
