Abstract:
:Spermatogenesis is a highly regulated process during which haploid sperm cells are generated. Although autophagy is involved in the spermatogenesis process, the molecular pathways and regulations of autophagy in germ cell development remain elusive. Here, we showed that Ppp1r36, a regulatory subunit of protein phosphatase 1, is expressed during gonadal development, mainly in testes during spermatogenesis. Autophagy protein LC3 (microtubule associated protein 1 light chain 3), especially its active form LC3-II, had a similar expression pattern to Ppp1r36. Moreover, LC3-II level and puncta analysis showed that autophagy is up-regulated around 21 dpp (day postpartum) in postnatal testis, indicating a potential role of autophagy during the first wave of spermatogenesis. We demonstrated that Ppp1r36 promotes autophagosome formation upon starvation induction. Further autophagy flux analysis using a tandem fluorescent indicator, mCherry-GFP-LC3, confirmed that Ppp1r36 participated in autophagy. We further determined that Ppp1r36 is associated with Atg16L1 (autophagy related 16-like 1) in autophagy of starvation induction. Thus, our results uncover a potential role of the regulatory subunit Ppp1r36 of protein phosphatase 1 in enhancing autophagy during spermatogenesis.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Zhang Q,Gao M,Zhang Y,Song Y,Cheng H,Zhou Rdoi
10.1038/srep24609subject
Has Abstractpub_date
2016-04-21 00:00:00pages
24609issn
2045-2322pii
srep24609journal_volume
6pub_type
杂志文章abstract::Gluconobacter oxydans sorbitol dehydrogenase (GoSLDH) exhibits a higher catalytic efficiency than other L-sorbose producing enzymes. During the reaction catalysed by GoSLDH, NADP+ is reduced to NADPH and D-sorbitol is oxidized to L-sorbose. However, GoSLDH activity is inhibited by the NADPH (Ki = 100 μM) formed during...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37401-0
更新日期:2019-01-28 00:00:00
abstract::Application of acute kidney injury (AKI) biomarkers with consideration of nonrenal conditions and systemic severity has not been sufficiently determined. Herein, urinary neutrophil gelatinase-associated lipocalin (NGAL), L-type fatty acid-binding protein (L-FABP) and nonrenal disorders, including inflammation, hypoper...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33077
更新日期:2016-09-08 00:00:00
abstract::Polymerized ionic liquids (polyILs) combine the unique properties of ionic liquids (ILs) with macromolecular polymers. But anion diffusivities in polyILs can be three orders of magnitude lower than that in ILs. Endeavors to improve ion transport in polyILs urgently need in-depth insights of ion transport in polyILs. A...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64689-8
更新日期:2020-05-08 00:00:00
abstract::Herein, we investigated the neurite pathfinding on electrospun microfibers with various fiber densities, diameters, and microbead islands, and demonstrated the development of 3D connected artificial neuronal network within a nanofiber-microbead-based porous scaffold. The primary culture of rat hippocampal embryonic ne...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08231-3
更新日期:2017-08-10 00:00:00
abstract::Splenic immune function was enhanced in diet-induced-obese (DIO) mice caused by Escherichia coli. The changes in spleen function on apoptosis were still unknown. Two hundred mice in groups Lean-E. coli and DIO-E. coli were intranasal instillation of E. coli. And another two hundred mice in groups Lean-PBS and DIO-PBS ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62044-5
更新日期:2020-03-20 00:00:00
abstract::The reprogramming of differentiated cells into induced pluripotent stem cells (iPSCs) can be achieved by ectopic expression of defined transcription factors (Oct3/4, Sox2, Klf4 and c-Myc). However, to date, some iPSCs have been generated using viral vectors; thus, unexpected insertional mutagenesis in the target cells...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07323
更新日期:2014-12-04 00:00:00
abstract::Directional replacement and directional non-replacement models are two alternative paradigms for community development in primary successional environments. The first model emphasizes turnover in species between early and late successional niches. The second emphasizes accumulation of additional diversity over time. T...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26360
更新日期:2016-05-31 00:00:00
abstract::Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide. We prospectively evaluated endothelial function by assessing flow-mediated dilatation (FMD) of the brachial artery in patients with biopsy-proven NAFLD. This prospective study included 139 patients (50 healthy controls, 47 patients wi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65835-y
更新日期:2020-06-01 00:00:00
abstract::In March/April 2020 the Italian government drastically reduced vehicle traffic and interrupted all non-essential industrial activities over the entire national territory. Italy thus became the first country in the world, with the exception of Hubei, to enact lockdown measures as a consequence of the COVID-19 outbreak ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66368-0
更新日期:2020-06-10 00:00:00
abstract::A unique combination of useful properties in boron-carbide, such as extreme hardness, excellent fracture toughness, a low density, a high melting point, thermoelectricity, semi-conducting behavior, catalytic activity and a remarkably good chemical stability, makes it an ideal material for a wide range of technological...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19330
更新日期:2016-01-18 00:00:00
abstract::We demonstrate a simple and effective strategy for implementing a polarizing beamsplitter for the terahertz spectral region, based on an artificial dielectric medium that is scalable to a range of desired frequencies. The artificial dielectric medium consists of a uniformly spaced stack of metal plates, which is elect...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06297-7
更新日期:2017-07-19 00:00:00
abstract::Goldlined spinefoot, Siganus guttatus, inhabits tropical and subtropical waters and synchronizes its spawning around the first quarter moon likely using an hourglass-like lunar timer. In previous studies, we have found that clock genes (Cryptochrome3 and Period1) could play the role of state variable in the diencephal...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24538-1
更新日期:2018-04-18 00:00:00
abstract::Shape memory alloys are typical temperature-sensitive metallic functional materials due to superelasticity and shape recovery characteristics. The conventional shape memory effect involves the formation and deformation of thermally induced martensite and its reverse transformation. The shape recovery process usually t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21754
更新日期:2016-02-16 00:00:00
abstract::Complex systems displaying recurrent spike patterns are ubiquitous in nature. Understanding the organization of these patterns is a challenging task. Here we study experimentally the spiking output of a semiconductor laser with feedback. By using symbolic analysis we unveil a nontrivial organization of patterns, revea...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04696
更新日期:2014-04-15 00:00:00
abstract::We assessed the association between depression status and prevalent and incident type 2 diabetes (T2D) as well as the interaction between depression and a genetic risk score (GS) based on 20 T2D single-nucleotide polymorphisms (SNPs) in a multi-ethnic longitudinal study. We studied 17,375 participants at risk for dysg...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01406-y
更新日期:2017-05-09 00:00:00
abstract::The formation of OD (order-disorder) phases of the LPSO (long-period stacking ordered)-type in Mg-Al-RE (RE (rare earth) = Y, La, Ce, Nd, Sm, Dy, Ho, Er and Yb) ternary systems has been investigated for both as-solidified and annealed conditions. The OD phase is found to form in those systems with RE = Y, Nd, Sm, Dy, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12506-0
更新日期:2017-09-25 00:00:00
abstract::Cancer vaccines are less effective at old than at young age because of immunosenescence. Besides, in preliminary observations we showed that the immunization with HER-2/neu DNA plasmid in transgenic young mice (standard immunization, SI) delays but not abrogate spontaneous mammary tumours progressively appearing durin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03286-8
更新日期:2017-06-08 00:00:00
abstract::The structure, mechanism of action and copper stoichiometry of the transmembrane sensor domain of the plant ethylene receptor ETR1 and homologs have remained elusive, hampering the understanding on how the perception of the plant hormone ethylene is transformed into a downstream signal. We generated the first structur...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45189-w
更新日期:2019-06-20 00:00:00
abstract::Influenza virus is a significant cause of morbidity and mortality, with worldwide seasonal epidemics. The duration and quality of humoral immunity and generation of immunological memory to vaccines is critical for protective immunity. In the current study, we examined the long-lasting protective efficacy of chimeric V...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40226
更新日期:2017-01-09 00:00:00
abstract::We aimed to explore the effects of type-2 diabetes mellitus (T2DM) and hypoglycemic therapy on the salivary microbiome in periodontitis patients and identify the potential salivary micro-biomarker for the early warning of T2DM. Saliva samples were collected from healthy individuals (Health), periodontitis patients (P)...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72035-1
更新日期:2020-09-21 00:00:00
abstract::Arbuscular mycorrhizal (AM) symbioses are an attractive means of improving the efficiency of soil phosphorus (P) that difficult to be used by plants and may provide a sustainable way of maintaining high yields while reducing P applications. However, quantifying the contribution of indigenous AM fungi on phosphorus upt...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80074-x
更新日期:2021-01-13 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-68522-0
更新日期:2020-07-10 00:00:00
abstract::In vertebrates, growth hormone/insulin-like growth factor (GH/IGF) axis signaling plays a critical role in regulating somatic growth. Understanding the direct upstream regulators of GH/IGF axis remains a major challenge. Our studies of the zebrafish reveal that the conserved miR-200 family members are critical regulat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15906
更新日期:2015-10-28 00:00:00
abstract::High frequency oscillations (HFOs) are bursts of neural activity in the range of 80 Hz or higher, recorded from intracranial electrodes during epileptiform discharges. HFOs are a proposed biomarker of epileptic brain tissue and may also be useful for seizure forecasting. Despite such clinical utility of HFOs, the spat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76138-7
更新日期:2020-11-05 00:00:00
abstract::Human KHDRBS1/Sam68 is an oncogenic splicing factor involved in signal transduction and pre-mRNA splicing. We explored the molecular mechanism of KHDRBS1 to be a prognostic marker in four different cancers. Within specific cancer, including kidney renal papillary cell carcinoma (KIRP), lung adenocarcinoma (LUAD), acut...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47558-x
更新日期:2019-07-31 00:00:00
abstract::Since its recent foundation, phase-coherent caloritronics has sparkled continuous interest giving rise to numerous concrete applications. This research field deals with the coherent manipulation of heat currents in mesoscopic superconducting devices by mastering the Josephson phase difference. Here, we introduce a new...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30268-1
更新日期:2018-08-16 00:00:00
abstract::Zooplankton provide the key link between primary production and higher levels of the marine food web and they play an important role in mediating carbon sequestration in the ocean. All commercially harvested fish species depend on zooplankton populations. However, spatio-temporal distributions of zooplankton are notor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37129-x
更新日期:2019-01-24 00:00:00
abstract::Photodynamic therapy is attracting increasing attention, but how to increase its tumor-specificity remains a daunting challenge. Herein we report a theranostic probe (azo-PDT) that integrates pyropheophorbide α as a photosensitizer and a NIR fluorophore for tumor imaging. The two functionalities are linked with a hypo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68847-w
更新日期:2020-07-22 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-24993-w
更新日期:2018-05-04 00:00:00
abstract::The beta rhythm (15-30 Hz) is a prominent signal of sensorimotor cortical activity. This rhythm is not sustained but occurs non-rhythmically as brief events of a few (1-2) oscillatory cycles. Recent work on the relationship between these events and sensorimotor performance suggests that they are the biologically relev...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54706-w
更新日期:2019-12-04 00:00:00