Abstract:
:Endothelial cells (ECs), that comprise the tumor vasculature, are critical targets for anticancer radiotherapy. The aim of this work was to study the mechanism by which SU5416, a known anti-angiogenesis inhibitor, modifies the radiation responses of human vascular ECs. Two human endothelial cell lines (HUVEC and 2H11) were treated with SU5416 alone, radiation alone, or a combination of both. In vitro tests were performed using colony forming assays, FACS analysis, western blotting, immunohistochemistry, migration assay, invasion assays and endothelial tube formation assays. The combination of radiation and SU5416 significantly inhibited cell survival, the repair of radiation-induced DNA damage, and induced apoptosis. It also caused cell cycle arrest, inhibited cell migration and invasion, and suppressed angiogenesis. In this study, our results first provide a scientific rationale to combine SU5416 with radiotherapy to target ECs and suggest its clinical application in combination cancer treatment with radiotherapy.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Kim EH,Kim MS,Jeong YK,Cho I,You SH,Cho SH,Lee H,Jung WG,Kim HD,Kim Jdoi
10.3892/ijo.2015.3127subject
Has Abstractpub_date
2015-10-01 00:00:00pages
1440-50issue
4eissn
1019-6439issn
1791-2423journal_volume
47pub_type
杂志文章abstract::Loss of heterozygosity (LOH) or allelic deletion at various loci has been reported in the majority of human tumors. The frequently deleted targets are believed to be tumor suppressor genes. Recent studies have identified the APC and MCC genes at 5q21, as putative tumor suppressor genes. The APC and MCC genes have been...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.1.111
更新日期:1995-01-01 00:00:00
abstract::As epidemiological data suggest ultraviolet (UV) radiation to be the major environmental factor for the development of melanoma, we screened this malignancy for UV-specific C right curved arrow T and CC right curved arrow TT DNA mutations as described in squamous and basal cell carcinomas of the skin. Mitochondrial (m...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::MicroRNAs can govern up to hundred different mRNAs and are important regulators of gene expression programs in development and disease. We analyzed the expression of microRNA-21, one of the most common oncomirs, in non-small cell lung cancer (NSCLC). Using northern blots the microRNA-21 expression levels of NSCLC-deri...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2272
更新日期:2014-04-01 00:00:00
abstract::Breast cancer cells can switch from estrogen receptor α (ER)- to human epidermal growth factor receptor (HER)-driven cell growth upon acquiring antiestrogen resistance. HER ligands are cleaved by metalloproteinases leading to release of active HER ligands, activation of HER receptors and consequently increased cell gr...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2434
更新日期:2014-07-01 00:00:00
abstract::A disintegrin and metalloproteinase-17 (ADAM17) can cut and release a wide variety of epidermal growth factor receptor (EGFR) ligands to promote survival, invasion and proliferation of cancer cell, and therefore, is considered to be a potential therapeutic target for cancer. The main goal of the present study was to o...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3536
更新日期:2016-08-01 00:00:00
abstract::Lung cancer is one of the most common types of cancer worldwide. Understanding the molecular mechanisms underlying the development and progression of lung cancer may improve early diagnosis, treatment and prognosis. The aim of the present study was to examine the pathogenesis of lung cancer and to identify potentially...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4926
更新日期:2020-01-01 00:00:00
abstract::Of 108 Wistar/Ma rats perinatally injected with Snyder-Theilen feline sarcoma virus, 75 developed tumors within 60 days (group A), and 25 after more than 117 days (group B). Metastases were detected in 59.1% and 11.8% in group A and B respectively. Antibody to v-fes gene product was found to occur before tumor develop...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.3.539
更新日期:1997-03-01 00:00:00
abstract::Toxicity of polyphenols against rat 3Y1 fibroblasts and the cells transformed by human adenovirus (Ad12-3Y1), its EIA gene (EIA-3Y1), or simian virus 40 (SV-3Y1) was examined. Among the diphenol compounds examined, pyrocatechol (o-diphenol) and hydroquinone (p-diphenol) showed selective toxicity against Ad12-3Y1 and E...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2.1.89
更新日期:1993-01-01 00:00:00
abstract::The retinoic acid receptor (RAR) beta gene is located in a region on chromosome 3p, which is frequently deleted in renal cell carcinoma. Since retinoic acid (RA) can inhibit cell proliferation and tumor formation, loss of RAR beta might contribute to oncogenesis of the kidney. This prompted us to examine RAR beta expr...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.2.391
更新日期:1995-02-01 00:00:00
abstract::The expression of RNA for interleukin (IL) -9, -10 and -12, interferon gamma (IFN-gamma), transforming growth factor beta one (TGF-beta1), macrophage inflammatory protein one alpha (MIP-1alpha) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in a panel of human T leukemia cell lines at various stages of ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.3.633
更新日期:1994-03-01 00:00:00
abstract::Our study reviews the central role of androgens for the development of androgen-insensitive growth in prostatic carcinoma using an individual dynamic model which emulates aspects of proliferation control. Our aim is the identification of individually proliferating cells from prostate carcinoma. Short-term cultured cel...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.6.1339
更新日期:1995-12-01 00:00:00
abstract::Diagnostic features of 102 consecutive cases of cancer reoccurrence in the conserved breast are reported. The sensitivity of palpation, mammography, cytology or ultrasonography was 75.5%, 58.7%, 77.4% and 77.8%, respectively. Mammography failures were not due to natural breast density and could probably be explained b...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.3.2.337
更新日期:1993-08-01 00:00:00
abstract::The P-glycoprotein (P-gp) multidrug transporter is present at the luminal face of the brain capillary endothelial cells that contribute to the blood-brain barrier. To study its role in transendothelial anticancer drug transport, we made use of a co-culture system formed of bovine brain capillary endothelial cells and ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract::E-cadherins are homophilic adhesion molecules the expression of which is tightly linked to the invasiveness and the differentiated state of the cells. E-cadherin expression seems also inversely related to the expression of vimentin, an intermediate filament implicated in the metastatic potential of some cells. In brea...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.1.181
更新日期:1994-01-01 00:00:00
abstract::Metastasis is an important prognosis factor in lung cancer, therefore, it is imperative to identify target molecules and elucidate molecular mechanism of metastasis for developing new therapeutics and diagnosis methods. We searched for metastasis-related proteins by utilizing a novel antibody proteome technology devel...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3000
更新日期:2015-07-01 00:00:00
abstract::Licochalcone A (LCA) is a natural product derived from the roots of Glycyrrhiza inflata exhibiting a wide range of bioactivities such as antitumor, anti-oxidant and anti-bacterial effects. Malignant pleural mesothelioma (MPM) is an extremely aggressive type of cancer with a poor prognosis because of its rapid progress...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2839
更新日期:2015-03-01 00:00:00
abstract::The p16(INK4A) gene is frequently inactivated in nonsmall cell lung carcinoma (NSCLC) by either mutations, deletions or DNA methylations. To assess the biological significance of p16(INK4A) inactivation in the development of NSCLC, full-length p16(INK4A) cDNA was introduced into NSCLC cell lines, A549 and H322, in whi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.1.33
更新日期:1997-01-01 00:00:00
abstract::The long-term survival for elderly patients with advanced ovarian papillary serous carcinoma (OPSC) does not exceed 30%, and the incidence and prognosis rise continuously after menopause. The aim of this study was to identify the differences in key miRNAs and their potential regulators through miRNA microarray analysi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1988
更新日期:2013-09-01 00:00:00
abstract::Manganese superoxide dismutase (MnSOD) seems to have a pivotal role in maintaining the normal phenotype by suppressing cell growth through blocking the entrance of quiescent cells into the cell cycle. MnSOD protein expression has been shown to be dysregulated in malignant cells. A well-established experimental breast ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.978
更新日期:2011-06-01 00:00:00
abstract::Chemo- and radiotherapy induce apoptosis in tumours and surrounding tissues. In a search for robust and reliable apoptosis markers, we have evaluated immunostaining patterns of gammaH2AX and cleaved PARP-1 in paraffin-embedded cellular spheroids. Breast cancer BT474 cells were grown as cell spheroids to diameters of 7...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000311
更新日期:2009-07-01 00:00:00
abstract::Although it is well established that ras genes contribute to tumourigenesis either through the accumulation of mutations or by aberrant expression in a wide range of human cancers, little is known regarding their involvement in human nasal polyps (NPs). In the present study, the occurrence of mutations in codons 11 an...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-11-01 00:00:00
abstract::Over-expression of p21Waf-1 was performed in 5 human glioma cell lines (U251MG, U87MG, T98G, NMC-G1, and KALS-1). p53 gene status was examined by PCR-SSCP and direct sequence method. The endogenous p21Waf-1 expression was evaluated by RT-PCR. Adenovirus-mediated over-expression of p21Waf-1 inhibited the proliferation ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.14.1.63
更新日期:1999-01-01 00:00:00
abstract::Identification of sensitive and specific biomarkers for early detection and prognosis of prostate cancer is essential for timely and appropriate treatment of the disease in individual patients. We identified an RNA transcript with sequence homology to TRPM8 (melastatin-related transient receptor potential member 8) th...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2010-02-01 00:00:00
abstract::Reports of the metabolomic characteristics of esophageal cancer are limited. In the present study, we thus conducted metabolome analysis of paired tumor tissues (Ts) and non-tumor esophageal tissues (NTs) using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). The Ts and surrounding NTs were surgi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4340
更新日期:2018-06-01 00:00:00
abstract::Hereditary nonpolyposis colorectal cancer (HNPCC) is a syndrome involving a predisposition to cancers of the colon, endometrium and several other extra-colonic sites, accounting for approximately 1-5% of all colorectal cancer cases. It is not easily recognized because of a lack of distinctive clinical markers, making ...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.12.4.947
更新日期:1998-04-01 00:00:00
abstract::The non-invasive treatment of tumors with preserved fertility holds great promise. The application of pulsed electric field (PEF) is a new biomedical engineering technique for tumor therapy. Picosecond pulsed electric fields (psPEF) can be transferred to target deep tissue non-invas...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1774
更新日期:2013-03-01 00:00:00
abstract::Although the incidence of brain metastasis in gastric cancer is relatively low, its prevalence may increase with improved therapy and longer survival tumors. The molecular mechanisms underlying brain metastases are not well understood. To gain insight into the mechanism of brain metastasis, we studied differences in m...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2680
更新日期:2014-12-01 00:00:00
abstract::Macrophages activated by lipopolysaccharide or interferon-gamma have been shown to be cytotoxic to tumor cells by releasing nitric oxide. Here, we report that unstimulated rat alveolar macrophages cultured with certain tumor cells produce nitric oxide and are cytotoxic to these tumor cells. Alveolar macrophages were t...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2.6.1053
更新日期:1993-06-01 00:00:00
abstract::Cancer stem cells (CSC) exhibit therapy resistance and drive self-renewal of the tumour, making cancer stem cells an important target for therapy. The PI3K signalling pathway has been the focus of considerable research effort, including in glioblastoma (GBM), a cancer that is notoriously resistant to conventional ther...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3510
更新日期:2016-07-01 00:00:00
abstract::Employing the transgenic BALB-neuT mouse tumor model, we explored the in vivo biologic relevance of immunocompetent epitopes shared among the four ErbB receptors. The outcome of neu-mediated tumorigenesis was compared following vaccination with isogeneic normal rat ErbB2/Neu (LTR-Neu) or xenogeneic human ErbB receptor...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-02-01 00:00:00