Abstract:
:Endothelial cells (ECs), that comprise the tumor vasculature, are critical targets for anticancer radiotherapy. The aim of this work was to study the mechanism by which SU5416, a known anti-angiogenesis inhibitor, modifies the radiation responses of human vascular ECs. Two human endothelial cell lines (HUVEC and 2H11) were treated with SU5416 alone, radiation alone, or a combination of both. In vitro tests were performed using colony forming assays, FACS analysis, western blotting, immunohistochemistry, migration assay, invasion assays and endothelial tube formation assays. The combination of radiation and SU5416 significantly inhibited cell survival, the repair of radiation-induced DNA damage, and induced apoptosis. It also caused cell cycle arrest, inhibited cell migration and invasion, and suppressed angiogenesis. In this study, our results first provide a scientific rationale to combine SU5416 with radiotherapy to target ECs and suggest its clinical application in combination cancer treatment with radiotherapy.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Kim EH,Kim MS,Jeong YK,Cho I,You SH,Cho SH,Lee H,Jung WG,Kim HD,Kim Jdoi
10.3892/ijo.2015.3127subject
Has Abstractpub_date
2015-10-01 00:00:00pages
1440-50issue
4eissn
1019-6439issn
1791-2423journal_volume
47pub_type
杂志文章abstract::The effects of different substrate stiffness were investigated on epithelial-mesenchymal transition (EMT) of cervical cancer cell lines and the role of miR-106b and its target protein DAB2 therein. Cervical cancer cell lines HeLa and SiHa were cultured on artificial substrates with different stiffness prepared using d...
journal_title:International journal of oncology
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journal_title:International journal of oncology
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更新日期:2002-09-01 00:00:00
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更新日期:2009-05-01 00:00:00
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