Abstract:
:Due to the increasing concern of using smallpox virus as biological weapons for terrorist attack, there is renewed interest in studying the pathogenesis of human smallpox and development of new therapies. Animal models are highly demanded for efficacy and safety examination of new vaccines and therapeutic drugs. Here, we demonstrated that both wild type and immunodeficient rats infected with an engineered vaccinia virus carrying Firefly luciferase reporter gene (rTV-Fluc) could recapitulate infectious and clinical features of human smallpox. Vaccinia viral infection in wild type Sprague-Dawley (SD) rats displayed a diffusible pattern in various organs, including liver, head and limbs. The intensity of bioluminescence generated from rTV-Fluc correlated well with viral loads in tissues. Moreover, neutralizing antibodies had a protective effect against virus reinfection. The recombination activating gene 2 (Rag2) knockout rats generated by transcription activator-like effector nucleases (TALENs) technology were further used to examine the infectivity of the rTV-Fluc in immunodeficient populations. Here we demonstrated that Rag2-/- rats were more susceptible to rTV-Fluc than SD rats with a slower virus clearance rate. Therefore, the rTV-Fluc/SD rats and rTV-Fluc/Rag2-/- rats are suitable visualization models, which recapitulate wild type or immunodeficient populations respectively, for testing human smallpox vaccine and antiviral drugs.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Liu Q,Fan C,Zhou S,Guo Y,Zuo Q,Ma J,Liu S,Wu X,Peng Z,Fan T,Guo C,Shen Y,Huang W,Li B,He Z,Wang Ydoi
10.1038/srep11397subject
Has Abstractpub_date
2015-08-03 00:00:00pages
11397issn
2045-2322pii
srep11397journal_volume
5pub_type
杂志文章abstract::In this paper, we prepared a novel structure to enhance the electroluminescence intensity from Si quantum dots/SiO2multilayers. An amorphous Si/SiO2 multilayer film was fabricated by plasma-enhanced chemical vapor deposition on a Pt nanoparticle (NP)-coated Si nanopillar array substrate. By thermal annealing, an embed...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11881
更新日期:2015-07-03 00:00:00
abstract::Transmission electron microscopy and related analytical techniques have been widely used to study the microstructure of different materials. However, few research works have been performed in the field of glasses, possibly due to the electron-beam irradiation damage. In this paper, we have developed a method based on ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17526
更新日期:2015-12-08 00:00:00
abstract::Direct growth of MoS2 nanowalls on vapor grown carbon nanofibers (VGCNFs) has been achieved using a microwave-assisted hydrothermal (MAH) method under an acidic condition. The acidic condition was obtained through the addition of an HCl aqueous solution. We demonstrate that the HCl not only modifies the pH value for l...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05805-z
更新日期:2017-07-20 00:00:00
abstract::Resolving the immunogenicity of cells derived from induced pluripotent stem cells (iPSCs) remains an important challenge for cell transplant strategies that use banked allogeneic cells. Thus, we evaluated the immunogenicity of mouse fetal neural stem/progenitor cells (fetus-NSPCs) and iPSC-derived neural stem/progenit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13522-w
更新日期:2017-10-11 00:00:00
abstract::The response of olfactory receptor neurons to odor mixtures is not well understood. Here, using experimental constraints, we investigate the mathematical structure of the odor response space and its consequences. The analysis suggests that the odor response space is 3-dimensional, and predicts that the dose-response c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24091
更新日期:2016-04-07 00:00:00
abstract::Multi-stranded helices are widespread in nature. The interplay of polymeric properties with biological function is seldom discussed. This study probes analogies between structural and mechanical properties of collagen and DNA. We modeled collagen with Eulerian rotational and translational parameters of adjacent rungs ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12878-3
更新日期:2017-10-16 00:00:00
abstract::The family of materials defined as ZrSiX (X = S, Se, Te) has been established as Dirac node-line semimetals, and subsequent study is urgent to exploit the promising applications of unusual magnetoresistance (MR) properties. Herein, we systematically investigated the anisotropic MR in the newly-discovered Dirac node-li...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27148-z
更新日期:2018-06-19 00:00:00
abstract::Following facial nerve axotomy, nerve function is not fully restored even after reconstruction. This may be attributed to axon degeneration/neuronal death and sustained neuroinflammation. CD38 is an enzyme that catalyses the hydrolysis of nicotinamide adenine dinucleotide (NAD+) and is a candidate molecule for regulat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73984-3
更新日期:2020-10-20 00:00:00
abstract::Parkinson disease (PD) is characterized by a pivotal progressive loss of substantia nigra dopaminergic neurons and aggregation of α-synuclein protein encoded by the SNCA gene. Genome-wide association studies identified almost 100 sequence variants linked to PD in SNCA. However, the consequences of this genetic variabi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54865-w
更新日期:2019-12-05 00:00:00
abstract::Algae-bacteria interaction is one of the main factors underlying the formation of harmful algal blooms (HABs). The aim of this study was to develop a genome-wide high-throughput screening method to identify HAB-influenced specific interactive bacterial metabolites using a comprehensive collection of gene-disrupted E. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67322-w
更新日期:2020-06-30 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-68128-6
更新日期:2020-07-06 00:00:00
abstract::Transcription factors (TFs) binding to specific DNA sequences or motifs, are elementary to the regulation of transcription. The gene is regulated by a combination of TFs in close proximity. Analysis of co-TFs is an important problem in understanding the mechanism of transcriptional regulation. Recently, ChIP-seq in ma...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43597
更新日期:2017-02-27 00:00:00
abstract::In surface science and model catalysis, cerium oxide (ceria) is mostly grown as an ultra-thin film on a metal substrate in the ultra-high vacuum to understand fundamental mechanisms involved in diverse surface chemistry processes. However, such ultra-thin films do not have the contribution of a bulk ceria underneath, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21165
更新日期:2016-02-16 00:00:00
abstract::The North Sea is one of the most extensively studied marine regions of the world. Hence, large amounts of molecular data for species identification are available in public repositories, and expectations to find numerous new species in this well-known region are rather low. However, molecular reference data for harpact...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45718-7
更新日期:2019-06-24 00:00:00
abstract::Esophageal and gastric cancers share some risk factors. This study aimed to compare the long-term trends in mortality rates of esophageal and gastric cancers in China to provide evidence for cancer prevention and control. Mortality data were derived from 103 continuous points of the Disease Surveillance Points system ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07071-5
更新日期:2017-07-28 00:00:00
abstract::Earthquakes are important natural events, yet their impacts on animal communities are poorly known. Understanding earthquake impacts on groundwater communities is essential to assess their resilience and hence to perform conservation actions. We investigated how a 6.3 Mw earthquake that occurred in 2009 altered the co...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20011-1
更新日期:2018-01-24 00:00:00
abstract::Neuropsychological assessment of human visual processing capabilities strongly depends on visual testing conditions including room lighting, stimuli, and viewing-distance. This limits standardization, threatens reliability, and prevents the assessment of core visual functions such as visual processing speed. Increasin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37016
更新日期:2016-11-21 00:00:00
abstract::An obstacle for precision improvement in quantum metrology is the information loss causing by the unavoidable interaction between probe system and environment. Quantum fluctuations are environment no system can be isolated from and it will make the precision of initial parameter estimation of the probe atom decrease w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00544-7
更新日期:2017-03-24 00:00:00
abstract::Since the re-emergence of Zika virus in 2014 and subsequent association with microcephaly, much work has focused on the development of a vaccine to halt its spread throughout the world. The mosquito vector that transmits this virus is widespread and responsible for the spread of other arboviridae including Dengue. Cur...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05980-z
更新日期:2017-07-24 00:00:00
abstract::On an SiO2-patterned c-plane sapphire substrate, GaN domains were grown with their polarity controlled in accordance with the pattern. While N-polar GaN was grown on hexagonally arranged circular openings, Ga-polar GaN was laterally overgrown on mask regions due to polarity inversion occurring at the boundary of the c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22424-4
更新日期:2018-03-07 00:00:00
abstract::The synthesis of highly biocompatible polymers is important for modern biotechnologies and medicine. Here, we report a unique process based on a two-step high-pressure ramp (HPR) for the ultrafast and efficient bulk polymerization of 2-(hydroxyethyl)methacrylate (HEMA) at room temperature without photo- and thermal ac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18244
更新日期:2015-12-16 00:00:00
abstract::The Y-box proteins are multifunctional nucleic acid-binding proteins involved in various aspects of gene regulation. The founding member of the Y-box protein family, YB-1, functions as a transcription factor as well as a principal component of messenger ribonucleoprotein particles (mRNPs) in somatic cells. The nuclear...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24401-3
更新日期:2018-04-18 00:00:00
abstract::Diffusion processes are widespread in biological and chemical systems, where they play a fundamental role in the exchange of substances at the cellular level and in determining the rate of chemical reactions. Recently, the classical picture that portrays diffusion as random uncorrelated motion of molecules has been re...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14486
更新日期:2015-09-30 00:00:00
abstract::A novel suite of instrumentation for the characterisation of materials held inside an air-tight tube furnace operated up to 250 °C has been developed. Real-time detection of released gases (volatile organic compounds (VOCs), CO2, NO, NO2, SO2, CO and O2) was achieved combining commercial off-the-shelf (COTS) gas senso...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65472-5
更新日期:2020-05-26 00:00:00
abstract::Migration and interactions of immune cells are routinely studied by time-lapse microscopy of in vitro migration and confrontation assays. To objectively quantify the dynamic behavior of cells, software tools for automated cell tracking can be applied. However, many existing tracking algorithms recognize only rather sh...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39725-x
更新日期:2019-03-01 00:00:00
abstract::Direct phenological mismatch caused by climate change can occur in mammals that moult seasonally. Two colour morphs of the weasel Mustela nivalis (M. n.) occur sympatrically in Białowieża Forest (NE Poland) and differ in their winter pelage colour: white in M. n. nivalis and brown in M. n. vulgaris. Due to their small...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26057-5
更新日期:2018-05-24 00:00:00
abstract::The purpose of this study was to evaluate changes in the prevalence and risk factors of cognitive impairment (CI) by analyzing and comparing two cross-sectional epidemiological surveys of CI and its subtypes were performed in a rural area of northern China between 2010 and 2015. Residents aged ≥60 years were drawn in ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37286-z
更新日期:2019-01-29 00:00:00
abstract::In present study, transmission electron microscopy and flow cytometry were utilized to investigate the classification, characterization and immune functions of hemocytes from horseshoe crab, Tachypleus tridentatus and Carcinoscorpius rotundicauda. Three types of hemocytes were distinguished respectively: the granular ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43630-8
更新日期:2019-05-08 00:00:00
abstract::α-synuclein (α-syn) is the main component of Lewy bodies, which are neuropathological hallmarks of patients with Parkinson's disease. As it has been controversial whether human α-syn from erythrocytes exists as a tetramer under physiological conditions, we tried solving this issue by the small-angle X-ray solution sca...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30473
更新日期:2016-07-29 00:00:00
abstract::Small ruminants, including sheep and goats are natural hosts of scrapie, and the progression of scrapie pathogenesis is strongly influenced by polymorphisms in the prion protein gene (PRNP). Although Korean native goats have been consumed as meat and health food, the evaluation of the susceptibility to scrapie in thes...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51621-y
更新日期:2019-10-25 00:00:00