Abstract:
:Migration and interactions of immune cells are routinely studied by time-lapse microscopy of in vitro migration and confrontation assays. To objectively quantify the dynamic behavior of cells, software tools for automated cell tracking can be applied. However, many existing tracking algorithms recognize only rather short fragments of a whole cell track and rely on cell staining to enhance cell segmentation. While our previously developed segmentation approach enables tracking of label-free cells, it still suffers from frequently recognizing only short track fragments. In this study, we identify sources of track fragmentation and provide solutions to obtain longer cell tracks. This is achieved by improving the detection of low-contrast cells and by optimizing the value of the gap size parameter, which defines the number of missing cell positions between track fragments that is accepted for still connecting them into one track. We find that the enhanced track recognition increases the average length of cell tracks up to 2.2-fold. Recognizing cell tracks as a whole will enable studying and quantifying more complex patterns of cell behavior, e.g. switches in migration mode or dependence of the phagocytosis efficiency on the number and type of preceding interactions. Such quantitative analyses will improve our understanding of how immune cells interact and function in health and disease.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Al-Zaben N,Medyukhina A,Dietrich S,Marolda A,Hünniger K,Kurzai O,Figge MTdoi
10.1038/s41598-019-39725-xsubject
Has Abstractpub_date
2019-03-01 00:00:00pages
3317issue
1issn
2045-2322pii
10.1038/s41598-019-39725-xjournal_volume
9pub_type
杂志文章abstract::This article presents the homodyne detection in a self-oscillation system, which represented by a short-range radar (SRR) circuit, that is analysed using a multi-time forced oscillator (MTFO) model. The MTFO model is based on a forced oscillation perspective with the signal and system theory, a second-order differenti...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43680
更新日期:2017-03-02 00:00:00
abstract::In the Brown et al. study 'Increased food supply mitigates ocean acidification effects on calcification but exacerbates effects on growth' they show disagreement with the tested hypothesis and data analysis methodology used in our 2016 study. We acknowledge careful criticism and a constructive dialogue are necessary t...
journal_title:Scientific reports
pub_type: 评论,杂志文章
doi:10.1038/s41598-018-27670-0
更新日期:2018-06-28 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-31524-0
更新日期:2018-09-03 00:00:00
abstract::Coronaviruses may exert severely negative effects on the mortality and morbidity of birds and mammals including humans and domestic animals. Most recently CoVID-19 has killed about half million people (27th of June, 2020). Susceptibility to this disease appears to differ markedly across different societies but the fac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69351-x
更新日期:2020-07-27 00:00:00
abstract::In this study, we developed a novel poly (lactic-co-glycolic acid)-dextran (PLD)-based nanodelivery system to enhance the anticancer potential of cisplatin (CDDP) in osteosarcoma cells. A nanosized CDDP-loaded PLGA-DX nanoparticle (PLD/CDDP) controlled the release rate of CDDP up to 48 h. In vitro cytotoxicity assay s...
journal_title:Scientific reports
pub_type: 杂志文章,收录出版
doi:10.1038/srep17387
更新日期:2015-12-01 00:00:00
abstract::Early treatment of single sided deafness in children has been recommended to protect from neurodevelopmental preference for the better hearing ear and from social and educational deficits. A fairly homogeneous group of five young children (≤3.6 years of age) with normal right sided hearing who received a cochlear impl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17129-z
更新日期:2017-12-04 00:00:00
abstract::Auricle defects are important and common occurrences in forensic medicine. The accurate measurement and assessment of auricle defects is key to identifying and evaluating injury, and the currently available methods are known to be labor intensive and inaccurate. In this paper, we introduce an identification and docume...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21289-x
更新日期:2018-02-12 00:00:00
abstract::Changes of intracellular and extracellular pH are involved in a variety of physiological and pathological processes, in which regulation of the Ca(2+) release activated Ca(2+) channel (I CRAC) by pH has been implicated. Ca(2+) entry mediated by I CRAC has been shown to be regulated by acidic or alkaline pH. Whereas se...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16747
更新日期:2015-11-18 00:00:00
abstract::The use of O-(2-[18F]fluoroethyl)-L-tyrosine ([18F]FET) as a positron emission tomography (PET) tracer for brain tumor imaging might have some limitations because of the relatively low affinity for the L-type amino acid transporter 1 (LAT1). To assess the stereospecificity and evaluate the influence of aromatic ring m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40013-x
更新日期:2019-02-27 00:00:00
abstract::Coccolithophores are single-celled photosynthesizing marine algae, responsible for half of the calcification in the surface ocean, and exert a strong influence on the distribution of carbon among global reservoirs, and thus Earth's climate. Calcification in the surface ocean decreases the buffering capacity of seawate...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34263
更新日期:2016-09-28 00:00:00
abstract::Here, we report a newly designed knee plug to be used in the 3rd generation of Autologous Chondrocyte Implantation (ACI) in order to heal the damaged knee cartilage. It is composed of three components: The first component (Bone Portion) is a 3D printed hard scaffold with large pores (~ 850 µm), made by hydroxyapatite ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73863-x
更新日期:2020-10-12 00:00:00
abstract::Nicotinamide phosphoribosyltransferase (NAMPT) and nicotinate phosphoribosyltransferase domain containing 1 (NAPRT1) are the main human NAD salvage enzymes. NAD regulates energy metabolism and cell signaling, and the enzymes that control NAD availability are linked to pathologies such as cancer and neurodegeneration. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06311
更新日期:2014-09-09 00:00:00
abstract::Volcanic activity is often preceded or accompanied by different types of seismo-volcanic signals. Among these signals, the so-called tornillo (Spanish for "screw") events are considered to belong to a unique class of volcano-seismicity characterised by a long-duration coda, amplitude modulation and high-quality factor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43842-y
更新日期:2019-05-14 00:00:00
abstract::The molecular mechanisms underlying viral pathogenesis are yet poorly understood owed to the large number of factors involved and the complexity of their interactions. Could we identify a minimal set of host transcription factors (TF) whose misregulation would result in the transcriptional profile characteristic of in...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01006
更新日期:2012-01-01 00:00:00
abstract::Hepatocyte spheroids are useful models for mimicking liver phenotypes in vitro because of their three-dimensionality. However, the lack of a biomaterial platform which allows the facile manipulation of spheroid cultures on a large scale severely limits their application in automated high-throughput drug safety testing...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61699-4
更新日期:2020-03-16 00:00:00
abstract::β-Lactamases are a major threat to the clinical use of carbapenems, which are often antibiotics of last resort. Despite this, the reaction outcomes and mechanisms by which β-lactamases degrade carbapenems are still not fully understood. The carbapenem bicyclic core consists of a β-lactam ring fused to a pyrroline ring...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49264-0
更新日期:2019-09-20 00:00:00
abstract::Recent research has shown that the cerebellum is involved not only in motor control but also in higher-level activities, which are closely related to creativity. This study aimed to explore the role of the cerebellum in visual divergent thinking based on its intrinsic activity. To this end, we selected the resting-sta...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73679-9
更新日期:2020-10-06 00:00:00
abstract::Recent advances in RNA-seq methodologies from limiting amounts of mRNA have facilitated the characterization of rare cell-types in various biological systems. So far, however, technical variations in these methods have not been adequately characterized, vis-à-vis sensitivity, starting with reduced levels of mRNA. Here...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03678
更新日期:2014-01-14 00:00:00
abstract::Encephalitis is a hallmark of Nipah virus (NiV) infection in humans. The exact route of entry of NiV into the central nervous system (CNS) is unknown. Here, we performed a spatio-temporal analysis of NiV entry into the CNS of hamsters. NiV initially predominantly targeted the olfactory epithelium in the nasal turbinat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00736
更新日期:2012-01-01 00:00:00
abstract::We investigate theoretically the two-photon absorption (TPA) for circular graphene quantum dots (GQDs) with the edge of armchair and zigzag on the basis of electronic energy states obtained by solving the Dirac-Weyl equation numerically under finite difference method. The expressions for TPA cross section are derived ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33260
更新日期:2016-09-15 00:00:00
abstract::Haloarchaea are unique microorganism's resistant to environmental and osmotic stresses and thrive in their habitats despite extreme fluctuating salinities. In the present study, haloarchaea were isolated from hypersaline thalossohaline salterns of Bhandup, Mumbai, India and were identified as Haloferax prahovense, Hal...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25642
更新日期:2016-05-27 00:00:00
abstract::Maize is rapidly replacing traditionally cultivated dual purpose crops of South Asia, primarily due to the better economic remuneration. This has created an impetus for improving maize for both grain productivity and stover traits. Molecular techniques can largely assist breeders in determining approaches for effectiv...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80118-2
更新日期:2021-01-12 00:00:00
abstract::HIV subtypes or clades differentially induce HIV-associated neurocognitive disorders (HAND) and substance abuse is known to accelerate HIV disease progression. The HIV-1 envelope protein gp120 plays a major role in binding and budding in the central nervous system (CNS) and impacts dopaminergic functions. However, the...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11130
更新日期:2015-06-09 00:00:00
abstract::Neural responses to incentives are altered in chronic pain and by opioid use. To understand how opioid use modulates the neural response to reward/value in chronic pain, we compared brain functional magnetic resonance imaging (fMRI) responses to a monetary incentive delay (MID) task in patients with fibromyalgia takin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45961-y
更新日期:2019-07-03 00:00:00
abstract::One procedure widely used to detect the velocity of a moving object is by using the Doppler effect. This is the perceived change in frequency of a wave caused by the relative motion between the emitter and the detector, or between the detector and a reflecting target. The relative movement, in turn, generates a time-v...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02815
更新日期:2013-10-02 00:00:00
abstract::Plant traits can influence the interactions between herbivore arthropods and their natural enemies. In these interactions generalist predators are often present, preying on herbivores and also on other arthropods in the same trophic guild. Variation in the strength of intraguild predation (IGP) may be related to habit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14997
更新日期:2015-10-09 00:00:00
abstract::Halyomorpha halys is a global invasive species, native to Southeast Asia, that is threatening agriculture in invaded regions. Our objectives were to: 1) establish the attractiveness of semiochemical stimuli paired with field-deployed traps in Europe (Greece, Hungary, Italy, and Switzerland), compared with Maryland, US...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17233-0
更新日期:2017-12-05 00:00:00
abstract::Marine plant communities such as kelp forests produce significant amounts of detritus, most of which is exported to areas where it can constitute an important trophic subsidy or potentially be sequestered in marine sediments. Knowing the vertical transport speed of detrital particles is critical to understanding the p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-34721-z
更新日期:2018-11-21 00:00:00
abstract::Ichthyoarchaeological evidence is uncommon at ancient hunter-gatherer sites from various regions and timeframes. This research contributes to the development of microarchaeological techniques useful for identifying fishing economies in situations where classifiable bones are unavailable. Specifically, traces of heat a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03737-2
更新日期:2017-06-15 00:00:00
abstract::The inhibitors of CD26 (dipeptidyl peptidase-4; DPP4) have been widely prescribed to control glucose level in diabetic patients. DPP4-inhibitors, however, accumulate stromal cell-derived factor-1α (SDF-1α), a well-known inducer of vascular leakage and angiogenesis both of which are fundamental pathophysiology of diabe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29393
更新日期:2016-07-06 00:00:00