The Plasmodiophora brassicae genome reveals insights in its life cycle and ancestry of chitin synthases.

Abstract:

:Plasmodiophora brassicae causes clubroot, a major disease of Brassica oil and vegetable crops worldwide. P. brassicae is a Plasmodiophorid, obligate biotrophic protist in the eukaryotic kingdom of Rhizaria. Here we present the 25.5 Mb genome draft of P. brassicae, developmental stage-specific transcriptomes and a transcriptome of Spongospora subterranea, the Plasmodiophorid causing powdery scab on potato. Like other biotrophic pathogens both Plasmodiophorids are reduced in metabolic pathways. Phytohormones contribute to the gall phenotypes of infected roots. We report a protein (PbGH3) that can modify auxin and jasmonic acid. Plasmodiophorids contain chitin in cell walls of the resilient resting spores. If recognized, chitin can trigger defense responses in plants. Interestingly, chitin-related enzymes of Plasmodiophorids built specific families and the carbohydrate/chitin binding (CBM18) domain is enriched in the Plasmodiophorid secretome. Plasmodiophorids chitin synthases belong to two families, which were present before the split of the eukaryotic Stramenopiles/Alveolates/Rhizaria/Plantae and Metazoa/Fungi/Amoebozoa megagroups, suggesting chitin synthesis to be an ancient feature of eukaryotes. This exemplifies the importance of genomic data from unexplored eukaryotic groups, such as the Plasmodiophorids, to decipher evolutionary relationships and gene diversification of early eukaryotes.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Schwelm A,Fogelqvist J,Knaust A,Jülke S,Lilja T,Bonilla-Rosso G,Karlsson M,Shevchenko A,Dhandapani V,Choi SR,Kim HG,Park JY,Lim YP,Ludwig-Müller J,Dixelius C

doi

10.1038/srep11153

subject

Has Abstract

pub_date

2015-06-18 00:00:00

pages

11153

issn

2045-2322

pii

srep11153

journal_volume

5

pub_type

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