Direct infusion mass spectrometry metabolomics dataset: a benchmark for data processing and quality control.

Abstract:

:Direct-infusion mass spectrometry (DIMS) metabolomics is an important approach for characterising molecular responses of organisms to disease, drugs and the environment. Increasingly large-scale metabolomics studies are being conducted, necessitating improvements in both bioanalytical and computational workflows to maintain data quality. This dataset represents a systematic evaluation of the reproducibility of a multi-batch DIMS metabolomics study of cardiac tissue extracts. It comprises of twenty biological samples (cow vs. sheep) that were analysed repeatedly, in 8 batches across 7 days, together with a concurrent set of quality control (QC) samples. Data are presented from each step of the workflow and are available in MetaboLights. The strength of the dataset is that intra- and inter-batch variation can be corrected using QC spectra and the quality of this correction assessed independently using the repeatedly-measured biological samples. Originally designed to test the efficacy of a batch-correction algorithm, it will enable others to evaluate novel data processing algorithms. Furthermore, this dataset serves as a benchmark for DIMS metabolomics, derived using best-practice workflows and rigorous quality assessment.

journal_name

Sci Data

journal_title

Scientific data

authors

Kirwan JA,Weber RJ,Broadhurst DI,Viant MR

doi

10.1038/sdata.2014.12

subject

Has Abstract

pub_date

2014-06-10 00:00:00

pages

140012

issn

2052-4463

journal_volume

1

pub_type

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