Abstract:
:Serial electron microscopy techniques have proven to be a powerful tool in biology. Unfortunately, the data sets they generate lack robust and accurate automated segmentation algorithms. In this data descriptor publication, we introduce a serial focused ion beam scanning electron microscopy (FIB-SEM) dataset consisting of six outer hair cell (OHC) stereocilia bundles, and the supranuclear part of the hair cell bodies. Also presented are the manual segmentations of stereocilia bundles and the gold bead labeling of PKHD1L1, a coat protein of hair cell stereocilia important for hearing in mice. This depository includes all original data and several intermediate steps of the manual analysis, as well as the MATLAB algorithm used to generate a three-dimensional distribution map of gold labels. They serve as a reference dataset, and they enable reproduction of our analysis, evaluation and improvement of current methods of protein localization, and training of algorithms for accurate automated segmentation.
journal_name
Sci Datajournal_title
Scientific dataauthors
Ivanchenko MV,Cicconet M,Jandal HA,Wu X,Corey DP,Indzhykulian AAdoi
10.1038/s41597-020-0509-4subject
Has Abstractpub_date
2020-06-17 00:00:00pages
182issue
1issn
2052-4463pii
10.1038/s41597-020-0509-4journal_volume
7pub_type
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