Abstract:
:Tumstatin (Tum) is a powerful angiostatin that inhibits proliferation and induces apoptosis of tumorous vascular endothelial cells. A nonpathogenic and anaerobic bacterium, Bifidobacterium longum (BL), selectively localizes to and proliferates in the hypoxia location within solid tumor. The aims of this study were to develop a novel delivery system for Tum using engineered Bifidobacterium and to investigate the inhibitory effect of Tum on tumor in mice. A vector that enabled the expression of Tum under the control of the pBBADs promoter of BL was constructed and transformed into BL NCC2705 by electroporation. The mouse colon carcinoma cells CT26 (1 × 10(7)/mL) were subcutaneously inserted in the left armpit of BALB/c mice. The tumor-bearing mice were treated with Tum-transformed BL, and green fluorescent protein (GFP)-transformed BL was used as a negative control. The microvessel density (MVD) in the transplanted tumor was determined, and terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end labeling was used to detect apoptosis of vascular endothelial cells in transplanted tumor. The in vitro expression of Tum was examined in BL after l-arabinose induction. Bifidobacterium longum with pBBAD-Tum (BL-Tum) showed significant antitumor effect in tumor-bearing mice. The weight, volume, growth, and MVD, as well as the percentage of apoptotic vascular endothelial cells of transplanted tumors in the tumor-bearing mice treated with Tum-transformed BL were all significantly lower than those in the GFP negative control group. Intragastric administration, injection in tumor and vena caudalis injection of Tum-transformed BL exerted marked antitumor effects in tumor-bearing mice. This is the first demonstration of the utilization of Tum-transformed BL as a specific gene delivery system for treating tumor.
journal_name
Technol Cancer Res Treatjournal_title
Technology in cancer research & treatmentauthors
Wei C,Xun AY,Wei XX,Yao J,Wang JY,Shi RY,Yang GH,Li YX,Xu ZL,Lai MG,Zhang R,Wang LS,Zeng WSdoi
10.1177/1533034615581977subject
Has Abstractpub_date
2016-06-01 00:00:00pages
498-508issue
3eissn
1533-0346issn
1533-0338pii
1533034615581977journal_volume
15pub_type
杂志文章abstract::Glucose transporter 1 (GLUT1) facilitates the cellular uptake of glucose and is overexpressed in most cancers. The altered expression of GLUT1 may influence the sensitivity of tumor cells to chemotherapy. This study investigated whether the knockdown of GLUT1 expression to sensitize head and neck cancer cells to the c...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500343
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND AND AIM:Methylated SEPT9 is a novel circulating tumor DNA marker for colorectal cancer, while the effects of various colorectal cancer clinicopathological factors on its detection performance have not been fully evaluated. This study aims to investigate the significance of the clinicopathological factors on ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033818791794
更新日期:2018-01-01 00:00:00
abstract:PURPOSE:The aim of the in vivo dosimetry, during the fractionated radiation therapy, is the verification of the correct dose delivery to patient. Nowadays, in vivo dosimetry procedures for photon beams are based on the use of the electronic portal imaging device and dedicated software to elaborate electronic portal ima...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033818779201
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND AND AIMS:To evaluate long-term outcomes and prognostic factors of laparoscopic microwave ablation as a first-line treatment for hepatocellular carcinoma located at the liver surface not feasible for percutaneous ablation. METHODS:51 consecutive patients receiving laparoscopic microwave ablation in our cente...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033818824338
更新日期:2019-01-01 00:00:00
abstract::Endometrial carcinoma is one of the most common cancers in women. A limited number of endometrial carcinoma cell lines are available for studies of signal transduction pathways and experimental therapeutics in vitro. However, these cell lines have not been comprehensively characterized. In this study, we used genome-w...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303461000900207
更新日期:2010-04-01 00:00:00
abstract:PURPOSE:The study was aimed to compare online 6 degree-of-freedom image registrations of TrueBeam cone-beam computed tomography and BrainLab ExacTrac X-ray imaging systems for intracranial radiosurgery. METHODS:Phantom and patient studies were performed on a Varian TrueBeam STx linear accelerator (version 2.5), which ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034616683069
更新日期:2017-06-01 00:00:00
abstract::Brachytherapy has the advantage of delivering a high dose to the tumor while sparing the surrounding normal tissues. With proper case selection and delivery technique, high-dose-rate (HDR) brachytherapy has great promise, because it eliminates radiation exposure, allows short treatment times, and can be performed on a...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章,评审
doi:10.1177/153303460400300305
更新日期:2004-06-01 00:00:00
abstract:BACKGROUND:Circulating tumor cells (CTCs) hold huge potential for both clinical applications and basic research into the management of cancer, but the relationship between CTC count and cervical cancer prognosis remains unclear. Therefore, research on this topic is urgently required. OBJECTIVE:This study investigated ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820957005
更新日期:2020-01-01 00:00:00
abstract::The accurate characterization and description of the vascular network of a cancer lesion is of paramount importance in clinical practice and cancer research in order to improve diagnostic accuracy or to assess the effectiveness of a treatment. The aim of this study was to show the effectiveness of liposomes as an ultr...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrtexpress.2013.600272
更新日期:2014-12-01 00:00:00
abstract::Results for treating glomus jugulare tumors with radiosurgery have been limited by short follow-up and small number of patients. We report our experience using LINAC or CyberKnife in 21 tumors with a median follow-up of 66 months (Mean follow-up of 60 months). In addition, we have a subset of eight patients that were ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460700600507
更新日期:2007-10-01 00:00:00
abstract:BACKGROUND:Antibody resistance, both de novo and acquired, is usually related to high risk of recurrence and lower survival rate in gynecologic cancers. Prevention or reversal of the resistance often yields beneficial clinical results. It was reported that anti-human epidermal growth factor receptor 3 monoclonal antibo...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034615588422
更新日期:2016-08-01 00:00:00
abstract::This study was designed to estimate the risk of radiation-associated tumors and clinical toxicity in the brain following fractionated radiation treatment of pituitary adenoma. A standard case of a patient with a pituitary adenoma was planned using 8 different dosimetric techniques. Total dose was 50.4 Gy (GyE) at dail...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500199
更新日期:2011-06-01 00:00:00
abstract::Chemo-radiotherapy is standard treatment of stage IIIA-N2 bulky or IIIB non-small cell lung cancer (NSCLC). Surgical resection of residual disease in downstaged patients may improve overall survival. In this setting, restaging disease is still a challenge. 18F-FDG PET/CT represents the gold standard although accuracy ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500437
更新日期:2015-12-01 00:00:00
abstract:OBJECTIVE:The incidence of colorectal cancer is increasing every year, and autophagy may be related closely to the pathogenesis of colorectal cancer. Autophagy is a natural catabolic mechanism that allows the degradation of cellular components in eukaryotic cells. However, autophagy plays a dual role in tumorigenesis. ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820984177
更新日期:2020-01-01 00:00:00
abstract::Traditional imaging techniques, such as computed tomography (CT), have high spatial resolution for changes in the anatomy or structure of organs, but are not very useful for detecting early biochemical changes. Conversely, positron emission tomography (PET) relies on changes in molecular biology to identify the earlie...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章,评审
doi:10.1177/153303460600500204
更新日期:2006-04-01 00:00:00
abstract:UNLABELLED:The purpose of this study was to compare the prognostic value of the percentage of positive biopsy cores (PPBC), the percentage of cancer volume (PCV), and the maximum involvement of biopsy cores (MIBC) as a prognostic factor in low- and intermediate-risk patients with clinically localized prostate cancer wh...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrtexpress.2013.600271
更新日期:2014-06-01 00:00:00
abstract:PURPOSE:We recently developed a novel, open-source in vivo dosimetry that uses the electronic portal imaging device to detect dose delivery discrepancies. We applied our method on patients with rectal cancer treated on a belly board device. METHODS:In vivo dosimetry was performed on 10 patients with rectal cancer trea...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034617711519
更新日期:2017-12-01 00:00:00
abstract:OBJECTIVE:Long noncoding RNAs (lncRNAs) are potential biomarkers for cancers. Nevertheless, the ability of long noncoding RNA lung cancer-associated transcript 1 in patients with multiple myeloma remains unknown. The purpose of this current study was to figure out its function in multiple myeloma. METHODS:Firstly, the...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820945770
更新日期:2020-01-01 00:00:00
abstract::Dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) is able to detect breast cancer with high sensitivity. Furthermore, this method provides functional information on tissue composition and vascularization. This study aims to identify the potential of DCE-MRI to predict distant metastasis in breast cancer p...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500266
更新日期:2012-12-01 00:00:00
abstract::Irreversible electroporation (IRE) has been shown to be an effective method of killing cells locally. In contrast to radiofrequency ablation, the mechanism by which cells are thought to die via IRE is the creation of pores in cell membranes, without substantial increase in tissue temperature. To determine the degree t...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrtexpress.2013.600253
更新日期:2014-12-01 00:00:00
abstract:PURPOSE:To evaluate 2 simultaneous integrated boost treatment planning techniques using helical tomotherapy for breast conserving therapy with regard to acute skin toxicity and dosimetry. METHODS:Thirty-two patients were studied. The original approach was for 16 patients and incorporated a directional block of the ips...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034615574387
更新日期:2016-04-01 00:00:00
abstract::The ultrasound-guided diffuse optical tomography is a noninvasive imaging technique for breast cancer diagnosis and treatment monitoring. The technique uses a handheld probe capable of providing measurements of multiple wavelengths in a few seconds. These measurements are used to estimate optical absorptions of lesion...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033818802791
更新日期:2018-01-01 00:00:00
abstract::Growing evidence suggests that aberration of the DNA repair pathway significantly contributes to tumorigenesis. Single-nucleotide polymorphisms in DNA repair-related genes such as WRN have been implicated in cancer risk. However, the results of published studies remain inconclusive. Therefore, we performed a meta-anal...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章,meta分析
doi:10.1177/1533034614561359
更新日期:2016-02-01 00:00:00
abstract:OBJECTIVE:To investigate the application value of apparent diffusion coefficient value in the pathological type, histologic grade, and presence of lymph node metastases of esophageal carcinoma. MATERIALS AND METHODS:Eighty-six patients with pathologically confirmed esophageal carcinoma were divided into different grou...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033819892254
更新日期:2019-01-01 00:00:00
abstract::Intraoperative magnetic resonance imaging has been applied to a number of neurosurgical disease processes since the late 1990's. The ability to visualize the operative site in near-real time has added a significant degree of safety to the treatment of lesions such as a cystic craniopharyngioma which can be located in ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460300200103
更新日期:2003-02-01 00:00:00
abstract::Numerous beam directions using 3-D conformal techniques can be employed in treating tumors in the posterior fossa, each with characteristic normal tissue exposure along the entrance and exit trajectory. A representative variety of beam configurations were modeled in a modern computer planning system initially with the...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460300200611
更新日期:2003-12-01 00:00:00
abstract::The purpose of this research is to establish a process of irradiating mice using the Gamma Knife as a versatile system for small animal irradiation and to validate accurate intracranial and extracranial dose delivery using this system. A stereotactic immobilization device was developed for small animals for the Gamma ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034616658394
更新日期:2017-04-01 00:00:00
abstract::We used a shifted-excitation Raman difference spectroscopy method for the ex vivo classification of resected and formalin-fixed breast tissue samples as normal (healthy) tissue, fibroadenoma, or invasive carcinoma. We analyzed 8 tissue samples containing invasive carcinoma that were surrounded by normal tissue and 3 t...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033818782532
更新日期:2018-01-01 00:00:00
abstract::Glypican-3 has been reported to be one of the most promising serum markers for hepatocellular carcinoma. This study aimed to assess the clinical utility of serum glypican 3 for the diagnosis of hepatocellular carcinoma. We recruited consecutive patients on a large scale, 283 with hepatocellular carcinoma, 445 with chr...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034615605248
更新日期:2016-12-01 00:00:00
abstract::Many conventional chemotherapeutic drugs, as well as DNA for cancer gene therapy, require efficient access to the cell interior to be effective. The cell membrane is a formidable barrier to many of these drugs, including therapeutic DNA constructs. Electropermeabilization (EP, often used synonymously with "electropora...
journal_title:Technology in cancer research & treatment
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1177/153303460200100110
更新日期:2002-02-01 00:00:00