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Micro-structured, spontaneously eroding hydrogels accelerate endothelialization through presentation of conjugated growth factors.

Abstract：

:Growth factors represent highly potent and highly efficacious means of communication to cells. At the same time, these proteins are fragile and relatively small sized--rendering their immobilization and controlled release from biomaterials challenging. In this work, we establish a method to incorporate growth factors into the physical hydrogels based on poly(vinyl alcohol), PVA. The latter have a long and successful history of biomedical applications and approval for diverse use in human patients, but are also characterized with scant opportunities for bioconjugation and functionalization. Herein, we develop the conjugation of growth factors to the micro-structured, spontaneously eroding physical hydrogels based on PVA. Protein conjugation was elaborated using model substrates, albumin and lysozyme, which aided to reveal specificity of chemical reactions and benign, non-harmful nature of the established protocols. Surface-adhered format of hydrogel analyses allowed to quantify bioconjugation reactions and enzymatic activity of the immobilized proteins and to visualize the hydrogels with immobilized cargo. In cell culture, immobilized growth factors were effective in communicating to adhering cells and specifically enhanced proliferation rates of the cells containing the corresponding receptors. At the same time, proliferation of the cells devoid of these receptors was un-altered.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Jensen BE,Edlund K,Zelikin AN

doi

10.1016/j.biomaterials.2015.01.036

subject

Has Abstract

pub_date

2015-05-01 00:00:00

pages

113-24

eissn

0142-9612

issn

1878-5905

pii

S0142-9612(15)00053-8

journal_volume

49

pub_type

杂志文章

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