Abstract:
:We have used a new synthetic injectable composite constituted of hydroxyapatite/tricalcium phosphate (HA/TCP) particles in suspension in a self-hardening Si-hydroxypropylmethylcellulose (HPMC) hydrogel. The aim of this study was to evaluate in vivo the biocompatibility and the new bone formation efficacy of this scaffold loaded with undifferentiated bone marrow stromal cells (BMSCs). This biomaterial was mixed extemporaneously with BMSCs prepared from C57BL/6 mice, injected in subcutaneous and intramuscular sites and retrieved 4 and 8 weeks after implantation. Dissection of the implants revealed a hard consistency and the absence of a fibrous capsule reflecting a good integration into the host tissues. Histological analysis showed mineralized woven bone in the granule inter-space with numerous active osteoclasts attached to the particles as assessed by the presence of multinucleated cells positively stained for TRAP activity and for the a3 subunit of the V-ATPase. Small vessels were homogenously distributed in the whole implants. Similar results were obtained in SC and IM sites and no bone formation was observed in the control groups when cell-free and particle-free transplants were injected. These results indicate that this injectable biphasic calcium phosphate-hydrogel composite mixed with undifferentiated BMSCs is a new promising osteoinductive bone substitute. It also provides with an original in vivo model of osteoclast differentiation and function.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Trojani C,Boukhechba F,Scimeca JC,Vandenbos F,Michiels JF,Daculsi G,Boileau P,Weiss P,Carle GF,Rochet Ndoi
10.1016/j.biomaterials.2006.01.057keywords:
subject
Has Abstractpub_date
2006-06-01 00:00:00pages
3256-64issue
17eissn
0142-9612issn
1878-5905pii
S0142-9612(06)00098-6journal_volume
27pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract::The kinetics of thrombin inhibition by antithrombin (AT) and heparin cofactor II (HC II) were analysed as a function of the heparin concentration, from 10(-9) to 10(-4) M. The initial concentrations of inhibitor (l) and thrombin (E) were set at equimolar levels (CI = CE = 10(-8) M). The experimental data indicate that...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(96)00125-1
更新日期:1997-02-01 00:00:00
abstract::ROCK activity increases due to ECM rigidity in the tumor microenvironment and promotes a malignant phenotype via actomyosin contractility. Invasive migration is facilitated by actin-rich adhesive protrusions known as invadopodia that degrade the ECM. Invadopodia activity is dependent on matrix rigidity and contractile...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2016.01.028
更新日期:2016-04-01 00:00:00
abstract::Based on the hypothesis that bone cements cause changes in the production of transforming growth factor-beta 1 (TGF-beta1) by bone cells, the effects of four acrylic bone cements (Sulfix-60, CMW 1, CMW 2 and CMW 3) were examined using the osteoblast-like cell line MG63. The extracts in MEM of the cements were tested, ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(01)00109-0
更新日期:2002-01-01 00:00:00
abstract::Collagen fibers are essential components of tissues, which are highly conserved across the animal kingdom and could be extremely useful in tissue engineering. The formation of these macromolecular fibers depends on molecular interactions-based self-assembly of the basic building blocks of collagen called tropocollagen...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.06.074
更新日期:2011-10-01 00:00:00
abstract::Hydrogels provide three-dimensional frameworks with tissue-like elasticity and high permeability for culturing therapeutically relevant cells or tissues. While recent research efforts have created diverse macromer chemistry to form hydrogels, the mechanisms of hydrogel polymerization for in situ cell encapsulation rem...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.08.083
更新日期:2011-12-01 00:00:00
abstract::Implantation of synthetic material, including vascular grafts, pacemakers, etc. results in the foreign body reaction and the formation of multinucleated giant cells (MGCs) at the exterior surface of the implant. Despite the long-standing premise that fusion of mononucleated macrophages results in the formation of MGCs...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2017.02.031
更新日期:2017-06-01 00:00:00
abstract::The goal of this work was to investigate the efficiency of transcellular delivery into Swiss albino mice 3T3 fibroblasts of molecules with various physico-chemical characteristics from ethosomes, phospholipid vesicular carriers containing ethanol. The probes chosen were: 4-(4-diethylamino) styryl-N-methylpyridinium io...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(01)00052-7
更新日期:2001-11-01 00:00:00
abstract::Many joint diseases are associated with the deposition of crystals in articular cartilage. Three main types are commonly found: calcium pyrophosphate, calcium hydroxyapatite and monosodium urate. The effects of these articular crystals on the frictional properties and the surface condition of the articular cartilage w...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(89)90074-4
更新日期:1989-07-01 00:00:00
abstract::Stimuli-responsive nanoparticles hold great promise for drug delivery to improve the safety and efficacy of cancer therapy. One of the most investigated stimuli-responsive strategies is to induce drug release by heating with laser, ultrasound, or electromagnetic field. More recently, cryosurgery (also called cryothera...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2018.07.021
更新日期:2018-10-01 00:00:00
abstract::One of the potential factors that may affect the results of mesenchymal stem cell (MSC)-based therapy is the age of donors and recipients. However, there have been no controlled studies to investigate the influence of skeletal maturity on the MSC-based repair of cartilage. The purpose of this study was to compare the ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.07.017
更新日期:2010-11-01 00:00:00
abstract::This study reports the effect of varying the thickness of a commercial zinc polycarboxylate dental cement on its adhesion in a metal-to-metal single lap joint. The thickness was controlled externally in a specially designed apparatus, rather than by the inclusion of isodimensional glass spheres, as has been done previ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(95)98279-n
更新日期:1995-01-01 00:00:00
abstract::Thermosensitive polymers poly(N-isopropylacrylamide) (PNIPAM), poly(N-vinylcaprolactam) (PVCL) and PVCL grafted with amphiphilic poly(ethylene oxide) (PEO) chains (PVCL-graft-C11EO42) were prepared and characterized and their putative cytotoxicity was evaluated. The cytotoxicity of these thermosensitive polymers and t...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2004.09.008
更新日期:2005-06-01 00:00:00
abstract::Hydroxyapatite ceramics with zirconia dispersion from fine powders synthesized hydrothermally were post-sintered at 1000-1300 degrees C under 200 MPa of argon for 1 h without capsules, after normal sintering in air at 1200 degrees C for 3 h. Densification was most significant with post-sintering at 1200 degrees C. Fra...
journal_title:Biomaterials
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::Among the many applications for carbon nanotubes (CNTs), their use in medicine has drawn special attention due to their potential for a variety of therapeutic and diagnostic applications. As progress toward clinical applications continues, monitoring CNTs in vivo will be essential to evaluate their biodistribution, po...
journal_title:Biomaterials
pub_type: 杂志文章,评审
doi:10.1016/j.biomaterials.2016.05.045
更新日期:2016-09-01 00:00:00
abstract::Retinal degenerations cause permanent visual loss and affect millions world-wide. Presently, a novel treatment highlights the potential of using biodegradable polymer scaffolds to induce differentiation and deliver retinal progenitor cells for cell replacement therapy. In this study, we engineered and analyzed a micro...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2009.02.046
更新日期:2009-07-01 00:00:00
abstract::A poly(urethane urea) (PUU) synthesized from 2,4-toluene diisocyanate (TDI) and polyethylene glycol (PEG) with ethylenediamine (ED) as the chain extender was rendered radiopaque by attaching 3,4,5-triiodobenzoic acid (TIB) onto the polymer backbone. The radiopaque polyurethane obtained was characterized by infra red (...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2007.03.025
更新日期:2007-07-01 00:00:00
abstract::Recent advances in biomaterial surface engineering have shown that surface biomechanical, spatial and topographical properties can elicit control over fundamental biological processes such as cell shape, proliferation, differentiation and apoptosis. Along these lines, we have very recently shown that the self-assembly...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2009.09.094
更新日期:2010-02-01 00:00:00
abstract::Circulating tumor cells (CTCs) are rare cells and the presence of these cells may indicate a poor prognosis and a high potential for metastasis. Despite highly promising clinical applications, CTCs have not been investigated thoroughly, due to many technical limitations faced in their isolation and identification. Cur...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.05.039
更新日期:2014-08-01 00:00:00
abstract::When developed as targeting ligands for the in vivo delivery of biomaterials to biological systems, RNA aptamers immediately face numerous obstacles, in particular nuclease degradation and post-selection 2' modification. This study aims to develop a novel class of highly stable, 2'-fully modified RNA aptamers that are...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.08.046
更新日期:2015-01-01 00:00:00
abstract::A-B-type block copolymers, consisting of polystyrene (PST) or poly(methyl methacrylate) (PMMA) forming segment (A) and poly(gamma-benzyl L-glutamate) (P[Glu(OBzl)]) segment (B), were synthesized and the thrombus formation on these block copolymer films was investigated in relation to the adsorption of plasma proteins ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(86)90010-4
更新日期:1986-09-01 00:00:00
abstract::The porous TiO2/perlite composite Ecopore is a synthetic biomaterial with possible clinical application in bone substitution. In our previous work, we demonstrated that surface modification of Ecopore with fibronectin (FN) enhanced spreading and growth of human osteoblasts in vitro. In the present study, we implanted ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2005.08.001
更新日期:2006-03-01 00:00:00
abstract::Vesicoureteral reflux and urinary incontinence can be treated by endoscopic injection. Injectables such as collagen and hyaluronic acid-dextranomer are commonly used, however are not offering optimal therapeutic effect yet. In the present study a novel application for poly(acrylonitrile)-based hydrogel (Hypan), alread...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2006.09.019
更新日期:2007-02-01 00:00:00
abstract::The suitability of chitosan-based hydrogels as scaffolds for the encapsulation of intervertebral disc (IVD) cells and the accumulation of a functional extracellular matrix mimicking that of the nucleus pulposus (NP) was investigated. The specific hypothesis under study was that the cationic chitosan would form an idea...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2005.06.037
更新日期:2006-01-01 00:00:00
abstract::Microencapsulation of dopamine-secreting cells in biocompatible, semi-permeable polymer membranes has been proposed as an alternative strategy for dopamine replacement for Parkinson's disease. In order to assess the viability of this proposal, dopamine-secreting PC12 cells were immunoisolated via microencapsulation in...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(97)00241-x
更新日期:1998-04-01 00:00:00
abstract::Two new formulations of bioactive glasses were used as coatings on titanium alloy (TiAl6V4) implants for prosthetic applications in the orthopaedic field. The biocompatibility of these bioglasses, as well as their osteoconductive properties, were assessed by employing primary cultures of human osteoblasts. A nonbioact...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(97)00249-4
更新日期:1998-06-01 00:00:00
abstract::Seven nominally identical samples of Na-free carbonate apatite (CO(3)Ap) were prepared by reaction of CaHPO(4) with ammonium carbonate solution at 70 degrees C over 3 days. They were studied by chemical analysis, Rietveld analysis of powder X-ray diffraction (XRD) data, Ca/P ratio determinations (quantitative phase an...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2006.04.033
更新日期:2006-09-01 00:00:00
abstract::The present study investigated the ectopic osteoinduction and early degradation of recombinant human bone morphogenetic protein-2 (rhBMP-2)-loaded porous beta-tricalcium phosphate (beta-TCP) in mice. The porous beta-TCP with 50 microg of rhBMP-2 (n = 25) and porous beta-TCP (control group, n = 25) were implanted into ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2004.10.035
更新日期:2005-07-01 00:00:00
abstract::Nitric oxide (NO) is an important biological messenger involved in the treatment of bacterial infections, but its controlled and targeted release in bacterial infections remains a major challenge. Herein, an intelligent NO nanogenerator triggered by near-infrared (NIR) light is constructed for targeted treatment of P....
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2020.120588
更新日期:2021-01-01 00:00:00
abstract::The polymerization of N,N' carbonylbis (caprolactam) (CBC) and polyol in the presence of alcoholate as catalyst produced cross-linked poly(ester-urea)s via ring opening addition reaction. In contrast to conventional synthetic routes, the use of non-toxic CBC eliminates the need for toxic isocyanate-based monomers. The...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2003.09.110
更新日期:2004-06-01 00:00:00
abstract::Block copolymer prodrugs (BCPs) have attracted considerable attentions in clinical translation of nanomedicine owing to their self-assembly into well-defined core-shell nanoparticles for improved pharmacokinetics, stability in blood circulation without drug leakage, and optimized biodistribution. However, a cascade of...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2017.11.006
更新日期:2018-02-01 00:00:00