Neural differentiation of pluripotent cells in 3D alginate-based cultures.

Abstract:

:Biomaterial-supported culture methods, allowing for directed three-dimensional differentiation of stem cells are an alternative to canonical two-dimensional cell cultures. In this paper, we evaluate the suitability of alginate for three-dimensional cultures to enhance differentiation of mouse embryonic stem cells (mESCs) towards neural lineages. We tested whether encapsulation of mESCs within alginate beads could support and/or enhance neural differentiation with respect to two-dimensional cultures. We encapsulated cells in beads of alginate with or without modification by fibronectin (Fn) or hyaluronic acid (HA). Gene expression analysis showed that cells grown in alginate and alginate-HA present increased differentiation toward neural lineages with respect to the two-dimensional control and to Fn group. Immunocytochemistry analyses confirmed these results, further showing terminal differentiation of neurons as seen by the expression of synaptic markers and markers of different neuronal subtypes. Our data show that alginate, alone or modified, is a suitable biomaterial to promote in vitro differentiation of pluripotent cells toward neural fates.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Bozza A,Coates EE,Incitti T,Ferlin KM,Messina A,Menna E,Bozzi Y,Fisher JP,Casarosa S

doi

10.1016/j.biomaterials.2014.02.039

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

4636-45

issue

16

eissn

0142-9612

issn

1878-5905

pii

S0142-9612(14)00195-1

journal_volume

35

pub_type

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