Abstract：
:The nanoscale materials properties of bone apatite crystals have been implicated in breast cancer bone metastasis and their interactions with extracellular matrix proteins are likely involved. In this study, we used geologic hydroxyapatite (HAP, Ca10(PO4)6(OH)2), closely related to bone apatite, to investigate how HAP surface chemistry and nano/microscale topography individually influence the crystal-protein interface, and how the altered protein deposition impacts subsequent breast cancer cell activities. We first utilized Förster resonance energy transfer (FRET) to assess the molecular conformation of fibronectin (Fn), a major extracellular matrix protein upregulated in cancer, when it adsorbed onto HAP facets. Our analysis reveals that both low surface charge density and nanoscale roughness of HAP facets individually contributed to molecular unfolding of Fn. We next quantified cell adhesion and secretion on Fn-coated HAP facets using MDA-MB-231 breast cancer cells. Our data show elevated proangiogenic and proinflammatory secretions associated with more unfolded Fn adsorbed onto nano-rough HAP facets with low surface charge density. These findings not only deconvolute the roles of crystal surface chemistry and topography in interfacial protein deposition but also enhance our knowledge of protein-mediated breast cancer cell interactions with apatite, which may be implicated in tumor growth and bone metastasis.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Wu F,Chen W,Gillis B,Fischbach C,Estroff LA,Gourdon Ddoi
10.1016/j.biomaterials.2016.11.043subject
Has Abstractpub_date
2017-02-01 00:00:00pages
174-185eissn
0142-9612issn
1878-5905pii
S0142-9612(16)30667-6journal_volume
116pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract：:Metastasis of cancer makes up the vast majority of cancer-related deaths, and it usually initiates from tumor cells invasiveness and develops through tumor neovasculature. In this work, we have fabricated a CD44/neuropilin dual receptor-targeting nanoparticulate system (tLyP-1-HT NPs) with endogenous or FDA approved c...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2017.05.022
更新日期：2017-08-01 00:00:00
abstract：:Pectin-derived matrices are now being examined and tested for controlled drug delivery. Pectin is intact in the upper gastrointestinal tract and degraded by colonic microflora. The composition of this microflora remains relatively consistent across a diverse human population. Thus, pectin-derived drug carriers provide...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/s0142-9612(03)00213-8
更新日期：2003-08-01 00:00:00
abstract：:Injectable hydrogel biomaterials are promising therapies to promote repair and regeneration post-myocardial infarction (MI). However, the timing of delivery and the mechanisms through which biomaterial treatments confer their benefits are translational issues that remain to be addressed. We assessed the efficacy of an...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.11.004
更新日期：2015-01-01 00:00:00
abstract：:Scaffolds derived from naturally occurring extracellular matrix (ECM) have found extensive use in the fields of tissue engineering and regenerative medicine. Many of these scaffolds are designed to degrade rapidly as they are replaced by new host tissue. Other scaffolds are chemically crosslinked to slow the rate of d...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2006.08.022
更新日期：2007-01-01 00:00:00
abstract：:The aim of this study was to characterize the relation of dimensional changes of dental ceramic materials as function of temperature. The thermal dimensional behavior of one glass ceramic core, four commercially available veneering porcelains and two experimental veneering porcelain materials was determined on cooling...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2003.09.027
更新日期：2004-05-01 00:00:00
abstract：:The tissue response to polyanionic collagen matrices, prepared from bovine pericardium and implanted subperiosteally in rat calvaria, was studied. The materials were implanted in 72 male rats (Rattus norvegicus, albinus, Holtzman), randomly divided into four groups: GI-MBP hydrolyzed for 24 h; GII-MBP hydrolyzed for 3...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(02)00292-2
更新日期：2003-01-01 00:00:00
abstract：:The development of highly funtional and orally available nanoparticles is the ultimate goal in nanoparticle delivery. Various functional nanoparticles have been studied to that end but there has yet to be an oral nanoparticle that can be successfully applied. Here, we describe for the first time a novel bile acid conj...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2017.09.022
更新日期：2017-12-01 00:00:00
abstract：:Self-assembly is a prominent phenomenon observed in nature. Inspired by this thermodynamically favorable approach, several natural and synthetic materials have been investigated to develop functional systems for various biomedical applications, including drug delivery. Furthermore, layered self-assembled systems provi...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/j.biomaterials.2020.119929
更新日期：2020-02-29 00:00:00
abstract：:The long-term patency rates of vascular grafts and stents are limited by the lack of surface endothelialisation of the implanted materials. We have previously reported that GRGDS and WQPPRARI peptide micropatterns increase the endothelialisation of prosthetic materials in vitro. To investigate the mechanisms by which ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.09.076
更新日期：2014-01-01 00:00:00
abstract：:Nitric oxide (NO) is an important biological messenger involved in the treatment of bacterial infections, but its controlled and targeted release in bacterial infections remains a major challenge. Herein, an intelligent NO nanogenerator triggered by near-infrared (NIR) light is constructed for targeted treatment of P....
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2020.120588
更新日期：2021-01-01 00:00:00
abstract：:Tissue engineering combined with gene therapy represents a promising approach for bone regeneration. The Hypoxia-inducible factor-1α (HIF-1α) gene is a pivotal regulator of vascular reactivity and angiogenesis. Our recent study has showed that HIF-1α could promote osteogenesis of bone mesenchymal stem cells (BMSCs) us...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.09.005
更新日期：2011-12-01 00:00:00
abstract：:Multifunctional and water-soluble superparamagnetic iron oxide (SPIO) nanocarriers were developed for targeted drug delivery and positron emission tomography/magnetic resonance imaging (PET/MRI) dual-modality imaging of tumors with integrin α(v)β₃ expression. An anticancer drug was conjugated onto the PEGylated SPIO n...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.02.006
更新日期：2011-06-01 00:00:00
abstract：:Adipose tissue engineering offers a promising alternative to the current breast reconstruction options. Here we investigated patient-specific breast scaffolds fabricated from poly(d,l)-lactide polymer with pore sizes>1 mm for their potential in long-term sustained regeneration of high volume adipose tissue. An optimis...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.01.025
更新日期：2015-06-01 00:00:00
abstract：:Bone repairs represent a major focus in orthopedic medicine with biomaterials as a critical aspect of the regenerative process. However, only a limited set of biomaterials are utilized today and few studies relate biomaterial scaffold design to degradation rate and new bone formation. Matching biomaterial remodeling r...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.04.028
更新日期：2010-08-01 00:00:00
abstract：:The aim of this study was to evaluate the bacterial adherence to biodegradable self-reinforced polyglycolic acid (SR-PGA) and self-reinforced poly-DL-lactic acid (SR-PLA 96) spiral stents in vitro. They are used as temporary urethral stents in urology. Gold-plated metal wire, polyurethane and latex were used as contro...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(97)00171-3
更新日期：1998-04-01 00:00:00
abstract：:Nanodiamond application in biotechnological and medical fields is nowadays in continuous progress. In fact, biocompatibility, reduced dimensions and high surface chemical interaction are specific features that make nanodiamonds perfect intracellular carriers of bioactive compounds. By confocal microscopy, we confirmed...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.10.057
更新日期：2015-01-01 00:00:00
abstract：:Collagenous xenografts made from kangaroo tail tendon cross-linked with glutaraldehyde have a potential application in the reconstruction of massive digital tendon deficits. However, a limitation to the clinical use of these xenografts has been the optimization of collagen cross-linking, and subsequent bio-incorporati...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(98)90187-9
更新日期：1999-06-01 00:00:00
abstract：:In order to develop a more effective self-etching primer, with a longer lasting shelf life, we designed a self-etching primer comprised of methacrylamide, N-methacryloyl glycine, NMGly. In this study, the hydrolytic stability of the amide portion in the NMGly was examined. The difference in the hydrolytic stability be...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(03)00616-1
更新日期：2004-03-01 00:00:00
abstract：:The coupling of specific antibodies to imaging agents often improves imaging specificity. However, free amine groups designed for the coupling can cause nonspecific binding of the imaging agents. We report here development of a nanocarrier, MnMEIO-silane-NH2-mPEG nanoparticles (NPs), consisting of a manganese-doped ir...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.02.025
更新日期：2013-05-01 00:00:00
abstract：:Sodium alginate hydrogel, stabilized with gelatin, is a suitable, biologically inert matrix that can be used for encapsulating and 3D bioprinting of bone-related SaOS-2 cells. However, the cells, embedded in this matrix, remain in a non-proliferating state. Here we show that addition of an overlay onto the bioprinted ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.07.002
更新日期：2014-10-01 00:00:00
abstract：:We genetically introduced the Fc-binding peptide (FcBP) into the loop of a self-assembled protein cage, ferritin, constituting four-fold symmetry at the surface to use it as a modular delivery nanoplatform. FcBP-presenting ferritin (FcBP-ferritin) formed very stable non-covalent complexes with both human and rabbit Ig...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2012.03.055
更新日期：2012-07-01 00:00:00
abstract：:Compared to soluble cytokines, surface-tethered ligands can deliver biological signalling with precise control of spatial positioning and concentration. A strategy that immobilises ligand molecules on a surface in a uniform orientation using non-cleavable linkages under physiological conditions would enhance the speci...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.10.029
更新日期：2015-01-01 00:00:00
abstract：:This study examined the engineering and true axial stress-strain behavior of smooth cylindrical and shallow and deep notched cylindrical test specimens, under applied axial tensile loading using non-contacting methods, of both conventional and highly crosslinked ultra-high molecular weight polyethylenes (UHMWPEs). The...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2004.09.031
更新日期：2005-06-01 00:00:00
abstract：:We have recently reported on an ultrafast degrading tyrosine-derived terpolymer that degrades and resorbs within hours, and is suitable for use in cortical neural prosthetic applications. Here we further characterize this polymer, and describe a new tyrosine-derived fast degrading terpolymer in which the poly(ethylene...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.04.052
更新日期：2011-08-01 00:00:00
abstract：:Large-scale expansion of highly functional adult human mesenchymal stem cells (aMSCs) remains technologically challenging as aMSCs lose self renewal capacity and multipotency during traditional long-term culture and their quality/quantity declines with donor age and disease. Identification of culture conditions enabli...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.01.081
更新日期：2014-04-01 00:00:00
abstract：:A series of mesoporous silica nanoparticles (MSNs) were synthesized using the co-structure directing method. A non-cytotoxic anionic surfactant, undec-1-en-11-yltetra(ethylene glycol) phosphate monoester surfactant (PMES), was used as a structure directing agent (SDA) together with aminopropyltrimethoxysilane that fun...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.04.077
更新日期：2011-09-01 00:00:00
abstract：:The anterior cruciate ligament (ACL) is the most commonly injured intra-articular ligament of the knee, and limitations in existing reconstruction grafts have prompted an interest in tissue engineered solutions. Previously, we reported on a tissue-engineered ACL scaffold fabricated using a novel, three-dimensional bra...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2004.11.050
更新日期：2005-08-01 00:00:00
abstract：:The authors prepared 7.5% and 12.5% collagen gel, and supplemented it with the tripeptide Gly-His-Lys (GHK), perfloxacine and hypersulphated glycosaminoglycan (HSGAG). By means of 125l marking, its absorption was followed from small polyurethane sponges placed under the skin of rats. The absorption of gel without HSGA...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(95)00310-x
更新日期：1996-08-01 00:00:00
abstract：:Thin sheets of nanofibrous (NF) poly(L-lactic acid) (PLLA) matrix were fabricated using a novel phase separation method, mimicking the structure of natural collagen fibers. In this study, the cell morphology, cytoskeleton and adhesion structure, proliferation and differentiation were investigated on NF PLLA matrix usi...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.06.015
更新日期：2008-10-01 00:00:00
abstract：:We hypothesized that reduced stem stiffness of orthopaedic implants contributes to a high risk of loosening, since interface stresses and relative motions may exceed a tolerable range. To study this hypothesis, three types of load-bearing implant with different stiffnesses were inserted into the tibia of the goat. His...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(97)00064-1
更新日期：1997-09-01 00:00:00
