Abstract：
:Scaffolds derived from naturally occurring extracellular matrix (ECM) have found extensive use in the fields of tissue engineering and regenerative medicine. Many of these scaffolds are designed to degrade rapidly as they are replaced by new host tissue. Other scaffolds are chemically crosslinked to slow the rate of degradation or add strength to the scaffold. Commercially available ECM scaffolds have considerable variability with regards to tissue origin and methods of processing, and little is known about their rate of degradation and the fate of their degradation products. A novel method is described herein to integrally label ECM with a radioactive isotope ((14)C). It was found that a number of tissues are efficiently labeled, including heart, liver, trachea, pancreas, small intestine, and urinary bladder tissue. Of the tissues analyzed, only spleen was not found to contain detectable levels of (14)C. The technique is extremely sensitive, accurate, and safe, but requires access to accelerator mass spectrometry, and is expensive and time consuming. This model represents the first described quantitative method to determine the rate of degradation for an ECM scaffold and to track the fate of the degradation products.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Gilbert TW,Stewart-Akers AM,Badylak SFdoi
10.1016/j.biomaterials.2006.08.022subject
Has Abstractpub_date
2007-01-01 00:00:00pages
147-50issue
2eissn
0142-9612issn
1878-5905pii
S0142-9612(06)00725-3journal_volume
28pub_type
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