Abstract：
:The rapid increase in the number of genetically modified (GM) varieties has led to a demand for high-throughput methods to detect genetically modified organisms (GMOs). We describe a new dynamic array-based high throughput method to simultaneously detect 48 targets in 48 samples on a Fludigm system. The test targets included species-specific genes, common screening elements, most of the Chinese-approved GM events, and several unapproved events. The 48 TaqMan assays successfully amplified products from both single-event samples and complex samples with a GMO DNA amount of 0.05 ng, and displayed high specificity. To improve the sensitivity of detection, a preamplification step for 48 pooled targets was added to enrich the amount of template before performing dynamic chip assays. This dynamic chip-based method allowed the synchronous high-throughput detection of multiple targets in multiple samples. Thus, it represents an efficient, qualitative method for GMO multi-detection.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Li X,Wu Y,Li J,Li Y,Long L,Li F,Wu Gdoi
10.1038/srep07616subject
Has Abstractpub_date
2015-01-05 00:00:00pages
7616issn
2045-2322pii
srep07616journal_volume
5pub_type
杂志文章abstract：:The interaction between the T cell antigen receptor (TCR) and antigenic peptide in complex with major histocompatibility complex (MHC) molecules is a crucial step in T cell activation. The relative contributions of TCR:peptide and TCR:MHC contacts to the overall binding energy remain unclear. This has important implic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35326
更新日期：2016-10-13 00:00:00
abstract：:A coherent anti-Stokes Raman scattering (CARS) rigid endoscope was developed to visualize peripheral nerves without labeling for nerve-sparing endoscopic surgery. The developed CARS endoscope had a problem with low imaging speed, i.e. low imaging rate. In this study, we demonstrate that noise reduction with deep learn...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72241-x
更新日期：2020-09-16 00:00:00
abstract：:During development sex differences in aromatase expression in limbic regions of mouse brain depend on sex chromosome factors. Genes on the sex chromosomes may affect the hormonal regulation of aromatase expression and this study was undertaken to explore that possibility. Male E15 anterior amygdala neuronal cultures e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05658-6
更新日期：2017-07-13 00:00:00
abstract：:In this paper, we investigate the magnetic behavior of a single-walled hexagonal spin-1 Ising nanotube by using the effective field theory (EFT) with correlations and the differential operator technique (DOT). The system consists of six long legs distributed parallel to each other on a hexagonal basis. Within each cha...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48833-7
更新日期：2019-08-26 00:00:00
abstract：:Sucralose is a widely-used artificial high-intensity sweetener. Although doubts have been raised about the safety of sucralose by several researchers, it can still be found in a broad range of foods and beverages worldwide, including in baked goods. Sucralose may decompose at high temperatures, and participate in chlo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02946
更新日期：2013-10-15 00:00:00
abstract：:Factor induced reprogramming of fibroblasts is an orchestrated but inefficient process. At the epigenetic level, it results in drastic chromatin changes to erase the existing somatic "memory" and to establish the pluripotent state. Accordingly, alterations of chromatin regulators including Ezh2 influence iPSC generati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08229
更新日期：2015-02-04 00:00:00
abstract：:Haemoparasites are responsible for some of the most prevalent and debilitating canine illnesses across the globe, whilst also posing a significant zoonotic risk to humankind. Nowhere are the effects of such parasites more pronounced than in developing countries in the tropics where the abundance and diversity of ectop...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49118-9
更新日期：2019-09-02 00:00:00
abstract：:The effects of sea salt aerosols (SSA) on cloud microphysical processes, precipitation, and upper troposphere/lower stratosphere water vapour in tropical cyclones were studied with the Weather Research and Forecasting with Chemistry model. Two numerical experiments were conducted: a control experiment (CTL) and an exp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51757-x
更新日期：2019-10-22 00:00:00
abstract：:Chemical reagents used in traditional mineral processing can be toxic and hazardous for the environment. Therefore, the use of biotechnological methods is becoming increasingly important. Great hopes are being placed in the use of microorganisms for bio-beneficiation of raw materials. However, assessment of adhesion a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49406-4
更新日期：2019-09-10 00:00:00
abstract：:Measurement techniques that allow the global analysis of cellular responses while retaining single-cell sensitivity are increasingly needed in order to understand complex and dynamic biological processes. In this context, compromises between sensitivity, degree of multiplexing, throughput, and invasiveness are often u...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53428-3
更新日期：2019-11-19 00:00:00
abstract：:This study was aimed to explore the bidirectional association between depression and peptic ulcers. The ≥20-year-old participants of the Korean National Health Insurance Service-National Sample Cohort from 2002 to 2013 were included in the study. In study I, 30,306 depression patients were 1:4 matched with 121,224 con...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-58783-0
更新日期：2020-02-04 00:00:00
abstract：:Endothelial cells (ECs) are major modulators of hemostasis by expressing and releasing pro- and anticoagulant mediators into the circulation. Previous studies showed that cultured ECs release procoagulant mediators into cell culture supernatants as evidenced by the reduction of viscoelastic clotting time. This effect ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03159-0
更新日期：2017-06-16 00:00:00
abstract：:Phelan McDermid Syndrome (PMDS) is a genetic disorder characterized by features of Autism spectrum disorders. Similar to reports of Zn deficiency in autistic children, we have previously reported high incidence of Zn deficiency in PMDS. However, the underlying mechanisms are currently not well understood. Here, using ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45190
更新日期：2017-03-27 00:00:00
abstract：:A dynamical system is controllable if by imposing appropriate external signals on a subset of its nodes, it can be driven from any initial state to any desired state in finite time. Here we study the impact of various network characteristics on the minimal number of driver nodes required to control a network. We find ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01067
更新日期：2013-01-01 00:00:00
abstract：:Sarcopenia is an aging condition involving low muscle mass and function. Fetuin-A (FetA) appears to be a factor for body composition remodeling. We hypothesized that age increases FetA levels and deteriorates the myocardial function by affecting diastolic function, especially in people with sarcopenia. We enrolled 541...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12078
更新日期：2015-07-10 00:00:00
abstract：:Sesquiterpene lactones (STL) are a subclass of isoprenoids with many known bioactivities frequently found in the Asteraceae family. In recent years, remarkable progress has been made regarding the biochemistry of STL, and today the biosynthetic pathway of the core backbones of many STLs has been elucidated. Consequent...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50520-6
更新日期：2019-10-04 00:00:00
abstract：:This study aims to assess the utility of internet search query analysis in pertussis surveillance. This study uses an empirical time series model based on internet search metrics to detect the pertussis incidence in Australia. Our research demonstrates a clear seasonal pattern of both pertussis infections and Google T...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11195-z
更新日期：2017-09-05 00:00:00
abstract：:The Tcf/Lef family of transcription factors mediates the Wnt/β-catenin pathway that is involved in a wide range of biological processes, including vertebrate embryogenesis and diverse pathogenesis. Post-translational modifications, including phosphorylation, sumoylation and acetylation, are known to be important for t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42590
更新日期：2017-02-15 00:00:00
abstract：:Three kinds of Al-TiO2 samples and pure TiO2 samples were synthesized via a modified polyacrylamide gel route using different aluminum salts, including Al2(SO4)3∙18H2O, AlCl3, and Al(NO3)3∙9H2O under identical conditions. The influence of different aluminum salts on the phase purity, morphologies, thermal stability of...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08216-2
更新日期：2017-08-14 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-71638-y
更新日期：2020-09-09 00:00:00
abstract：:In surface science and model catalysis, cerium oxide (ceria) is mostly grown as an ultra-thin film on a metal substrate in the ultra-high vacuum to understand fundamental mechanisms involved in diverse surface chemistry processes. However, such ultra-thin films do not have the contribution of a bulk ceria underneath, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21165
更新日期：2016-02-16 00:00:00
abstract：:Calcitriol, a metabolite of vitamin D, is often used in osteoporosis clinics. However, the material has other bioactivities; for example, it accelerates angiogenesis, has anti-inflammatory properties, and inhibits oxidative stress. We investigated the effects of calcitriol in a random skin flap rat model. "McFarlane f...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18945
更新日期：2016-01-06 00:00:00
abstract：:This corrects the article DOI: 10.1038/srep40037. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/srep46991
更新日期：2018-05-22 00:00:00
abstract：:Polyphenol oxidases (PPOs) comprise tyrosinases (TYRs) and catechol oxidases (COs), which catalyse the initial reactions in the biosynthesis of melanin. TYRs hydroxylate monophenolic (monophenolase activity) and oxidize diphenolic (diphenolase activity) substrates, whereas COs react only with diphenols. In order to el...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57671-x
更新日期：2020-02-03 00:00:00
abstract：:Bacteriophage T7 is an intracellular parasite that recognizes its host via its tail and tail fiber proteins, known as receptor-binding proteins (RBPs). The RBPs attach to specific lipopolysaccharide (LPS) features on the host. Various studies have shown expansion of the phage's host range via mutations in the genes en...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-57221-0
更新日期：2020-01-15 00:00:00
abstract：:Salinity is a critical abiotic stress, which significantly impacts the agricultural yield worldwide. Identification of the molecular mechanisms underlying the salt tolerance in euhalophyte Suaeda salsa is conducive to the development of salt-resistant crops. In the present study, high-throughput RNA sequencing was per...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61204-x
更新日期：2020-03-06 00:00:00
abstract：:G-quadruplexes (G4s) are nucleic acids secondary structures, epigenetic regulators in cells and viruses. In herpes simplex virus 1 (HSV-1)-infected cells, G4s are massively present during viral replication. We here aimed at investigating the possibility to target the HSV-1 G4s by a core extended naphtalene diimide (c-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02667-3
更新日期：2017-05-24 00:00:00
abstract：:Attraction to infants is a common feature of non-human primates. Frequent affiliative male-infant interactions have been observed in many multimale, multifemale groups of macaques, including a behaviour termed 'bridging' in which two male macaques simultaneously lift an infant. This behaviour has been suggested to ser...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34406-7
更新日期：2018-11-02 00:00:00
abstract：:Cytokines of the interleukin (IL)-1 family regulate immune and inflammatory responses. The recently discovered IL-36 family members are involved in psoriasis, rheumatoid arthritis, and pulmonary diseases. Here, we show that IL-36α interacts with heme thereby contributing to its regulation. Based on in-depth spectrosco...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53231-0
更新日期：2019-11-15 00:00:00
abstract：:Phytoplasmas (class, Mollicutes) are insect-transmissible and plant-pathogenic bacteria that multiply intracellularly in both plants and insects through host switching. Our previous study revealed that phytoplasmal sigma factor rpoD of OY-M strain (rpoDOY) could be a key regulator of host switching, because the expres...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11893
更新日期：2015-07-07 00:00:00
