当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Biochemical and structural characterization of Cryptosporidium parvum Lactate dehydrogenase.

Biochemical and structural characterization of Cryptosporidium parvum Lactate dehydrogenase.

Abstract：

:The protozoan parasite Cryptosporidium parvum causes waterborne diseases worldwide. There is no effective therapy for C. parvum infection. The parasite depends mainly on glycolysis for energy production. Lactate dehydrogenase is a major regulator of glycolysis. This paper describes the biochemical characterization of C. parvum lactate dehydrogenase and high resolution crystal structures of the apo-enzyme and four ternary complexes. The ternary complexes capture the enzyme bound to NAD/NADH or its 3-acetylpyridine analog in the cofactor binding pocket, while the substrate binding site is occupied by one of the following ligands: lactate, pyruvate or oxamate. The results reveal distinctive features of the parasitic enzyme. For example, C. parvum lactate dehydrogenase prefers the acetylpyridine analog of NADH as a cofactor. Moreover, it is slightly less sensitive to gossypol inhibition compared with mammalian lactate dehydrogenases and not inhibited by excess pyruvate. The active site loop and the antigenic loop in C. parvum lactate dehydrogenase are considerably different from those in the human counterpart. Structural features and enzymatic properties of C. parvum lactate dehydrogenase are similar to enzymes from related parasites. Structural comparison with malate dehydrogenase supports a common ancestry for the two genes.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Cook WJ,Senkovich O,Hernandez A,Speed H,Chattopadhyay D

doi

10.1016/j.ijbiomac.2014.12.019

subject

Has Abstract

pub_date

2015-03-01 00:00:00

pages

608-19

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(14)00823-X

journal_volume

74

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • Amphiphilic graft copolymeric micelle using dextrin and poly (N-vinyl caprolactam) via RAFT polymerization: Development and application.

    abstract：:Dextrin and poly (N-vinyl caprolactam) based amphiphilic graft copolymer has recently been developed using RAFT polymerization. The prepared graft copolymer has been characterized in details using FTIR and 1H NMR spectral analyses, GPC, TGA, FESEM, TEM and DLS analyses. GPC analysis results indicate that the polymeriz...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.07.198

    authors： Pal A,Sarkar AN,Karmakar PD,Pal S

    更新日期：2018-11-01 00:00:00

  • A polysaccharide from Hedyotis diffusa interrupts metastatic potential of lung adenocarcinoma A549 cells by inhibiting EMT via EGFR/Akt/ERK signaling pathways.

    abstract：:In this study we investigated the potential effects of a polysaccharide (HDP) from Hedyotis diffusa on the metastasis in human lung adenocarcinoma A549 cells. HDP (25, 50 and 100μg/ml) significantly suppressed the cell adhesion, invasion and migration of A549 cells in a dose dependent manner by downregulation of matri...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.02.040

    authors： Lin L,Cheng K,He Z,Lin Q,Huang Y,Chen C,Xie Z,Chen L,Liang Z

    更新日期：2019-05-15 00:00:00

  • Optimization of the culture conditions for production of Polyhydroxyalkanoate and its characterization from a new Bacillus cereus sp. BNPI-92 strain, isolated from plastic waste dumping yard.

    abstract：:The aim of this study was to optimize the culture conditions for production, recovery and characterization of polyhydroxyalkanoate (PHA) from potential Bacillus cereus strain BNPI-92. Cost effective carbon sources such as crude oil (CO), rice bran (RB), sugar cane molasses (SCM) and wheat bran (WB) were evaluated for ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.11.138

    authors： Mohammed S,Behera HT,Dekebo A,Ray L

    更新日期：2020-08-01 00:00:00

  • Bioactivity and biocompatibility of a chitosan-tobermorite composite membrane for guided tissue regeneration.

    abstract：:A polymer-mineral composite membrane of the mucopolysaccharide derivative, chitosan, and calcium silicate hydrate phase, tobermorite, was prepared by solvent casting and characterised by scanning electron microscopy (SEM) and Fourier Transform infrared spectroscopy (FTIR). The bioactivity and biocompatibility of the c...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.11.020

    authors： Hurt AP,Getti G,Coleman NJ

    更新日期：2014-03-01 00:00:00

  • Vital insights into prokaryotic genome compaction by nucleoid-associated protein (NAP) and illustration of DNA flexure angles at single-molecule resolution.

    abstract：:Integration Host Factor (IHF) is a heterodimeric site-specific nucleoid-associated protein (NAP), well known for its DNA bending ability. Although the IHF induced bending states of DNA have been captured by both X-ray Crystallography and Atomic Force Microscopy (AFM), the range of flexibility and degree of heterogenei...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.12.194

    authors： Purkait D,Bandyopadhyay D,Mishra PP

    更新日期：2021-01-05 00:00:00

  • Prohibitin as an oxidative stress biomarker in the eye.

    abstract：:Identification of biomarker proteins in the retina and retinal pigment epithelium (RPE) under oxidative stress may imply new insights into signaling mechanisms of retinal degeneration at the molecular level. Proteomic data from an in vivo mice model in constant light and an in vitro oxidative stress model are compared...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2010.08.018

    authors： Lee H,Arnouk H,Sripathi S,Chen P,Zhang R,Bartoli M,Hunt RC,Hrushesky WJ,Chung H,Lee SH,Jahng WJ

    更新日期：2010-12-01 00:00:00

  • Characterization and protective effect of Polygonatum sibiricum polysaccharide against cyclophosphamide-induced immunosuppression in Balb/c mice.

    abstract：:In this study, the polysaccharide from Polygonatum sibiricum (PSP) was evaluated for the immunomodulatory activity by the cyclophosphamide (Cy)-induced immunosuppressed-model in vivo. The PSP has been analyzed in order to identify a variety of chemical properties such as monosaccharide compositions and structural conf...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.09.051

    authors： Liu N,Dong Z,Zhu X,Xu H,Zhao Z

    更新日期：2018-02-01 00:00:00

  • Alginate based tamoxifen/metal dual core-folate decorated shell: Nanocomposite targeted therapy for breast cancer via ROS-driven NF-κB pathway modulation.

    abstract：:Breast cancer endocrine resistance prevents unleashing full capabilities of Tamoxifen (TMX), besides TMX off-target side effects on healthy tissue. In this study, we engineered TMX nanocomposite via co-loading it on alginate-based silver nanoparticles and embedding within folic acid-polyethylene glycol surface conjuga...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.12.266

    authors： Ibrahim OM,El-Deeb NM,Abbas H,Elmasry SM,El-Aassar MR

    更新日期：2020-03-01 00:00:00

  • Effect of salinity stress on bioactive compounds and antioxidant activity of wheat microgreen extract under organic cultivation conditions.

    abstract：:This study was conducted to confirm the effects of salinity stress on bioactive compounds and antioxidant activity of wheat microgreen extract. The microgreens were cultivated for 8 days in organic media with different concentrations of Na [0 (control), 12.5, 25, 50, and 100 mM from sodium chloride] which was containe...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.08.090

    authors： Islam MZ,Park BJ,Lee YT

    更新日期：2019-11-01 00:00:00

  • Adjustable 'cross-linking' of neighboring DNA molecules in liquid-crystalline dispersions through (daunomycin-copper) polymeric chelate complexes.

    abstract：:The interaction of daunomycin molecules with double-stranded DNA in the liquid-crystalline state was investigated. It was shown that at a certain extent of daunomycin binding a change of the mechanism of anthracycline orientation with reference to the DNA chain occurs. This is testified by the alteration of the sense ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/s0141-8130(96)01134-8

    authors： Yevdokimov YM,Salyanov VI,Spener F,Palumbo M

    更新日期：1996-12-01 00:00:00

  • Cellulosic paper with high antioxidative and barrier properties obtained through incorporation of tannin into kraft pulp fibers.

    abstract：:In this work, we prepared novel cellulosic paper by incorporating tannin into the kraft pulp for potential application in active food packaging. The kraft pulp fibers were firstly periodate oxidized to obtain the dialdehyde cellulosic fibers, and then reacted with varied dosages of tannin to incorporate them into the ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.06.101

    authors： Ji Y,Xu Q,Jin L,Fu Y

    更新日期：2020-11-01 00:00:00

  • Rapid analysis of polyhydroxyalkanoate contents and its monomer compositions by pyrolysis-gas chromatography combined with mass spectrometry (Py-GC/MS).

    abstract：:Here, we report an analysis method for determining PHA (polyhydroxyalkanoates) contents and their monomer composition in microbial cells based on pyrolysis gas chromatography combined with mass spectrometry (Py-GC/MS). Various kinds of microbial cells accumulating different PHA contents and monomer compositions were p...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2021.01.108

    authors： Khang TU,Kim MJ,In Yoo J,Sohn YJ,Jeon SG,Park SJ,Na JG

    更新日期：2021-01-21 00:00:00

  • Amylopectin-g-poly(methylacrylate-co-sodium acrylate): An efficient Cd(II) binder.

    abstract：:Synthesis, characterization and Cd(II) adsorption studies of a novel biodegradable graft copolymer based on partially hydrolysed polymethylacrylate (PMA) grafted amylopectin was reported, which was prepared by first grafting of PMA chains onto the amylopectin backbone followed by partial alkaline hydrolysis. The hydro...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.06.022

    authors： Sasmal D,Kolya H,Tripathy T

    更新日期：2016-10-01 00:00:00

  • Super-paramagnetic loaded nanoparticles based on biological macromolecules for in vivo targeted MR imaging.

    abstract：:Target-specific MRI contrast agent based on super-paramagnetic iron oxide-chitosan-folic acid (SPIONP-CS-FA) nanoparticles was fabricated by using an ionotropic gelation method, which involved the loading of SPIONPs at various concentrations into CS-FA nanoparticles by electrostatic interaction. The SPIONP-CS-FA nanop...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.01.049

    authors： Sanjai C,Kothan S,Gonil P,Saesoo S,Sajomsang W

    更新日期：2016-05-01 00:00:00

  • Identification of a novel alkaline amylopullulanase from a gut metagenome of Hermetia illucens.

    abstract：:A novel pullulanase gene, PulSS4, was identified from the gut microflora of Hermetia illucens by a function-based metagenome screening. The PulSS4 gene had an open reading frame of 4455 base pairs, and encoded a mature protein of 1484 amino acids, with a signal peptide sequence of 44 amino acids. The deduced amino aci...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.10.067

    authors： Lee YS,Seo SH,Yoon SH,Kim SY,Hahn BS,Sim JS,Koo BS,Lee CM

    更新日期：2016-01-01 00:00:00

  • Experimental and theoretical study of IBC domain from human IP3R2; molecular cloning, bacterial expression and protein purification.

    abstract：:IP3 is a ubiquitous second messenger in eukaryotic cells that triggers Ca2+-release from intracellular stores. IP3 binds to intracellular IP3-receptor (IP3R) and induces conformational change within the ligand-binding domain which regulates Ca2+-release; hence, both IP3 and IP3R are key components of the signal transd...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.09.117

    authors： Amroudie MN,Ataei F

    更新日期：2019-03-01 00:00:00

  • Antivirus and immune enhancement activities of sulfated polysaccharide from Angelica sinensis.

    abstract：:This study is to synthesize sulfated Angelica polysaccharides (APSs) and investigate the activity of one of the sulfated derivatives APS-1 on murine leukemia virus in vivo. Six sulfated derivatives with degree of sulfation ranging from 0.68 to 1.91 were obtained. And the virus replication was inhibited by APS-1 at the...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2011.11.027

    authors： Yang T,Jia M,Zhou S,Pan F,Mei Q

    更新日期：2012-04-01 00:00:00

  • Interaction of hexa-His tag with acidic amino acids results in facilitated refolding of halophilic nucleoside diphosphate kinase.

    abstract：:We have previously reported that amino-terminal extension sequence containing hexa-His facilitated refolding and assembly of hexameric nucleoside diphosphate kinase from extremely halophilic archaeon Halobacterium salinarum (NDK). In this study, we made various mutations in both the tag sequence and within NDK molecul...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2011.07.014

    authors： Ishibashi M,Ida K,Tatsuda S,Arakawa T,Tokunaga M

    更新日期：2011-11-01 00:00:00

  • The structure property and adsorption capacity of new enzyme-treated potato and sweet potato starches.

    abstract：:To explore a valuable new enzyme method that effectively changes the molecular structure and absorption property of potato and sweet potato starch granules, the common combination of α-amylase (AA) and glucoamylase (GA), namely AA → GA (common method as contrast), the combination of glycosyltransferase (GT) and GA, na...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.09.164

    authors： Guo L,Li J,Li H,Zhu Y,Cui B

    更新日期：2020-02-01 00:00:00

  • UV-mediated solid-state cross-linking of electrospinning nanofibers of modified collagen.

    abstract：:The modified collagen with double bonds was electrospun into nanofibers membrane cured with photo-initiated polymerization under UV irradiation. The morphology and chemical structures were characterized by SEM and FTIR. Both the water resistance and mechanical properties were improved after UV-crosslinking. Then 5-Flu...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.09.029

    authors： Song X,Dong P,Gravesande J,Cheng B,Xing J

    更新日期：2018-12-01 00:00:00

  • Starch-based polyurethane/CuO nanocomposite foam: Antibacterial effects for infection control.

    abstract：:In the present study, a new method for the synthesis of the open cell flexible polyurethane foams (PUFs) was developed by using starch powder and the modification of closed cell foam formulation. Starch is the second largest polymeric carbohydrate as a macromolecule on this planet with a large number of glucose units....

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.01.137

    authors： Ashjari HR,Dorraji MSS,Fakhrzadeh V,Eslami H,Rasoulifard MH,Rastgouy-Houjaghan M,Gholizadeh P,Kafil HS

    更新日期：2018-05-01 00:00:00

  • Synthesis of γ-cyclodextrin/chitosan composites for the efficient removal of Cd(II) from aqueous solution.

    abstract：:The synthesis of chitosan-graft-γ-cyclodextrin (Ch-g-γ-CD) using persulfate/ascorbic acid redox system was done and characterized by FTIR, XRD, TGA and SEM/EDX. The optimum yield of the copolymer was obtained using 16×10(-3) M γ-cyclodextrins (γ-CD), 2.8×10(-2) M ascorbic acid (AA), 1.8×10(-2) M K(2)S(2)O(8) and 0.1g ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2011.06.002

    authors： Mishra AK,Sharma AK

    更新日期：2011-11-01 00:00:00

  • Biophysical characterization of the interaction between M2-1 protein of hRSV and quercetin.

    abstract：:hRSV is the major causative agent of acute respiratory infections. Among its eleven proteins, M2-1 is a transcription antiterminator, making it an interesting target for antivirals. Quercetin is a flavonol which inhibits some virus infectivity and replication. In the present work, the M2-1 gene was cloned, expressed a...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.11.033

    authors： Teixeira TS,Caruso ÍP,Lopes BR,Regasini LO,Toledo KA,Fossey MA,Souza FP

    更新日期：2017-02-01 00:00:00

  • Characterization and antioxidant activities of the polysaccharides from Radix Cyathulae officinalis Kuan.

    abstract：:A water-soluble polysaccharide (PSRC) was extracted and purified from the roots of Radix Cyathulae officinalis Kuan, and its chemical characteristics, monosaccharide composition and antioxidant activities were characterized. The average of molecular weight (Mw) of PSRC was 182 kDa. The majority of monosaccharide compo...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.09.007

    authors： Han X,Shen S,Liu T,Du X,Cao X,Feng H,Zeng X

    更新日期：2015-01-01 00:00:00

  • The attenuation of HIV-1 Tat-induced neurotoxicity by Salvianic acid A and Danshen granule.

    abstract：:The neurotoxicity of HIV-1 Tat protein contributes significantly to the pathogenesis of HAND, and hence the attractive therapeutic strategies focusing on Tat-induced neurotoxicity are warranted. Salvia miltiorrhiza have been known to antioxidant property and neuroprotective effects. The Danshen granule is the pharmace...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.11.146

    authors： Bao D,Wang J,Liu J,Qin T,Liu H

    更新日期：2019-03-01 00:00:00

  • Overexpression of miR-335 inhibits the migration and invasion of osteosarcoma by targeting SNIP1.

    abstract：:MicroRNAs (miRNAs) are key regulators in the development and progression of osteosarcoma (OS). Based on our previous microarray data, we found that miR-335 expression was downregulated in OS tissue relative to normal tissue. Herein, we further found that miR-335 was downregulated in OS cell lines relative to the osteo...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.04.016

    authors： Xie Y,Deng H,Wei R,Sun W,Qi Y,Yao S,Cai L,Wang Y,Deng Z

    更新日期：2019-07-15 00:00:00

  • Isolation and characterization of lignocellulose nanofibers from different wheat straw pulps.

    abstract：:Wheat straw was cooked under different pulping processes: Soda (100°C, 7% NaOH, 150min), Kraft (170°C, 16% alkalinity, 25% sulfidity, 40min) and Organosolv (210°C, 60% ethanol, 60min). Once the pulps were obtained, lignocellulose nanofibers (LCNF) were isolated by mechanical process and TEMPO-mediated oxidation follow...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.08.019

    authors： Sánchez R,Espinosa E,Domínguez-Robles J,Loaiza JM,Rodríguez A

    更新日期：2016-11-01 00:00:00

  • Decolorization affects the structural characteristics and antioxidant activity of polysaccharides from Thesium chinense Turcz: Comparison of activated carbon and hydrogen peroxide decolorization.

    abstract：:Different decolorization methods have different effects on the properties of polysaccharides. In this study, polysaccharides extracted from Thesium chinense Turcz (T. chinense) were decolorized using either hydrogen peroxide (H2O2), which has been previously reported, or activated carbon. To begin, the conditions for ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.11.074

    authors： Shao L,Sun Y,Liang J,Li M,Li X

    更新日期：2020-07-15 00:00:00

  • Inhibition of normal and waxy corn starch retrogradation by sodium borohydride.

    abstract：:After gelatinization, starch is prone to retrograde during storage, and it is a notorious challenge to inhibit starch retrogradation in papermaking, textile, and construction fields. This work aimed to develop a new method for inhibiting the retrogradation of normal corn starch (CS) and waxy corn starch (WCS) by sodiu...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.03.017

    authors： Yu M,Xu Z,Ji N,Dai L,Xiong L,Sun Q

    更新日期：2020-06-15 00:00:00

  • The three novel complete aciniform spidroin variants from Araneus ventricosus reveal diversity of gene sequences within specific spidroin type.

    abstract：:Orb-weaving spiders produce multiple types of silks with distinct functions and material features, and all these silks are mainly composed of proteins which encoded by specific spidroin (spider silk protein) gene family members. Moreover, nearly all spidroins have evolved one or more variants within specific-species. ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.04.142

    authors： Wen R,Wang K,Meng Q

    更新日期：2020-08-15 00:00:00