Abstract：
:Here, we report an analysis method for determining PHA (polyhydroxyalkanoates) contents and their monomer composition in microbial cells based on pyrolysis gas chromatography combined with mass spectrometry (Py-GC/MS). Various kinds of microbial cells accumulating different PHA contents and monomer compositions were prepared through the cultivation of Ralstonia eutropha and recombinant Escherichia coli. Py-GC/MS could analyse these samples in a short time without complicated pretreatment steps. Characteristic peaks such as 2-butenoic acid, 2-pentenoic acid, and hexadecanoic acid regarding PHA compositions and cell components were identified. Considering constituents of cells and ratios of peak areas of dehydrated monomers to hexadecanoic acid, a simple equation for estimation of PHA contents in microbial cells was derived. Also, monomer compositions of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) in R. eutropha could be successfully determined based on peak area of 2-butenoic acid and 2-pentenoic acid of Py-GC/MS, which are the corresponding species of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) in PHBV. Correlation of results between GC-FID and Py-GC/MS could be fitted very well. This method shows similar results for the samples obtained from same experimental conditions, allowing rapid and reliable analysis. Py-GC/MS can be a promising tool to rapidly screen PHA-positive strains based on polymer contents along with monomer compositions.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Khang TU,Kim MJ,In Yoo J,Sohn YJ,Jeon SG,Park SJ,Na JGdoi
10.1016/j.ijbiomac.2021.01.108subject
Has Abstractpub_date
2021-01-21 00:00:00eissn
0141-8130issn
1879-0003pii
S0141-8130(21)00139-2pub_type
杂志文章abstract：:Substantial amounts of industrial, agricultural, medical and domestic water worldwide are polluted by different types of contaminants. Removing these contaminants from effluent by adsorbent materials made from abundant, inexpensive polysaccharides is a feasible and promising approach to deal with this problem. In the ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.03.091
更新日期：2017-08-01 00:00:00
abstract：:Two COS mixtures and a low molecular weight chitosan (LMWC) were tested for potential cytotoxicity and genotoxicity upon human lymphocytes. Genotoxicity was evaluated in vitro by cytokinesis-blocked micronucleus and alkaline comet assays, while cytotoxicity was assessed by flow cytometry analysis. Our results suggest ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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abstract：:In the present study, high-methoxyl pectin (HMP) was extracted from Hylocereus polyrhizus peel's using physico-chemical process. In addition, the hypolipidemic activity of HMP was investigated at different concentration and time corresponding to its adsorption ability. FTIR and contact angle analysis were used to dete...
journal_title：International journal of biological macromolecules
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abstract：:Miniature pigs are regarded as ideal organ donors for xenotransplantation into humans. Elucidating the formation mechanism of miniature pigs is important. The insulin-like growth factor 1 receptor (IGF-1R) is crucial in the regulation of cell proliferation and organismal growth. According to our previous research, the...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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abstract：:Biocompatible and biodegradable gelatin is a good candidate bioink for use in 3D bioprinting technologies, but viscous gelatin solution has a low printability. In order to improve the poor printability of gelatin, we optimized the rheological properties of gelatin solution. 3D gelatin scaffolds were then cross-linked ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2019-08-15 00:00:00
abstract：:The effects of osmolytes on the unfolding and refolding process of recombinant human brain-type creatine kinase (rHBCK) were comparatively, quantitatively studied in dilute solutions and macromolecular crowding systems (simulated by 100 g/L polyethylene glycol 2000), respectively. The results showed that the osmolytes...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.07.026
更新日期：2012-12-01 00:00:00
abstract：:Snail mucus is an attractive natural substance, which is increasingly used in cosmetic creams and syrups thanks to its emollient, moisturizing, protective and reparative properties. The aim of the present study was to explore the physicochemical properties of chitosan-based films added with snail mucus extracted from ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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abstract：:In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode. The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC)...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.06.019
更新日期：2015-09-01 00:00:00
abstract：:Chitosan nanoparticles have many applications, such as gene and drug delivery, due to their biocompatibility. Chitosan nanoparticles are currently produced by dissolution in acetic acid that affects the biocompatibility at acidic pH. Here, we synthesized and characterized chitosan (CS) and ascorbate chitosan (AsCS) na...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.04.055
更新日期：2018-08-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.01.101
更新日期：2016-05-01 00:00:00
abstract：:The aqueous polysaccharide from Polygonatum sibiricum was extracted and fractionated using anion-exchange chromatography to obtain F1 fraction. The F1 was chemically sulfated and partially acid-hydrolyzed for the production of its over-sulfated (OS1,2,3) and hydrolyzed (HP1,2,3) derivatives, in which the sulfate conte...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.10.119
更新日期：2019-02-01 00:00:00
abstract：:A C6-carboxylated curdlan (C6-Cc) obtained from 4-acetamido-TEMPO-mediated oxidation of curdlan was used both as a reducing and stabilizing agent for green synthesis of pH-responsive AuNPs, which was carried out by controlling the pH of the C6-Cc solution at a high temperature (100°C). C6-Cc presented a semi-flexible ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.08.029
更新日期：2018-01-01 00:00:00
abstract：:The effects of temperature-cycled retrogradation treatment on the structural characteristics and in vitro digestibility of waxy potato starch were investigated in this study. The results showed that the maximum yield of slowly digestible starch (SDS) in waxy potato starch reached 38.63% by retrogradation treatment und...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.03.007
更新日期：2014-06-01 00:00:00
abstract：:Fascioliasis, a serious helminth disease of the livestock population, results from infection with the parasite Fasciola. Despite the alarming increase in drug resistance, a safe and fully effective vaccine for fascioliasis is still not available. In the present study, we employed high-throughput immunoinformatics appr...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.07.024
更新日期：2019-10-01 00:00:00
abstract：:During carcinogenesis, changes in the glycosylation can modulate many biological processes. Thus, the interest in exploring and understanding the roles of carbohydrates as cancer biomarkers has been increasing. Lectins have been applied as useful tools in glycobiology, especially when associated with fluorescent repor...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.07.088
更新日期：2019-10-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.02.043
更新日期：2019-05-15 00:00:00
abstract：:The purpose of this study was to gain insights into the hydrolysis characteristics of xylanase in producing xylo-oligosaccharides and to improve these by protein engineering. In this study, a hybrid enzyme (T-XynFM) was created from Talaromyces thermophilus F1208 xylanase (T-Xyn) by replacement of the N-terminal exten...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.12.050
更新日期：2017-03-01 00:00:00
abstract：:In this study, ecofriendly starch/TiO2 bio-nanocomposites were produced using with different nano-TiO2 (TiO2) content (1, 3, and 5 (wt%)). Physical, mechanical, thermal, water-vapor permeability (WVP) properties and UV transmittance were investigated. Our results showed that the increasing TiO2 content increased the h...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.11.065
更新日期：2017-02-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.01.033
更新日期：2014-04-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(01)00172-6
更新日期：2001-12-10 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2006.03.026
更新日期：2006-11-15 00:00:00
abstract：:Six low molecular fucoidan (DFPS) derivatives were synthesized successfully, and their potential antioxidant activities were investigated employing various established in vitro systems. All DFPS derivatives possessed considerable antioxidant activity, and had stronger antioxidant ability than DFPS in certain tests. Th...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2009.02.001
更新日期：2009-06-01 00:00:00
abstract：:An analysis of the possible modes of motion of water molecules in polysaccharide gels indicates that the interpretation of NMR results does not require the supposition of a class of 'bound water' with highly perturbed motional states. There may, however, be water molecules associated with junction zones in the gels wh...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/s0141-8130(97)00045-7
更新日期：1997-08-01 00:00:00
abstract：:The pure chitosan nanofibrous membranes with average fiber diameter of 86±18, 114±17,164±28nm were successfully prepared by electrospinning. Batch adsorption experiments of using chitosan nanofibrous membranes as adsorbent to remove acid blue-113 were conducted. The adsorption capacity of 1377mg/g was achieved by the ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2018-01-01 00:00:00
abstract：:The research aims the development and optimization of capecitabine loaded interpenetrating polymeric network by ionotropic gelation method using polymers locust bean gum and sodium alginate by QbD approach. FMEA was performed to recognize the risks influencing CQAs. BBD was applied to study the effect of factors (poly...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.04.123
更新日期：2018-08-01 00:00:00
abstract：:This study evaluates the immunomodulating activities of Astragalus polysaccharide (APS) and sulfated APS (SAPS) in LPS-infected broiler chicks. SAPS was derived using the classic chlorosulfonic acid-pyridine method. On day 16, the birds were injected intramuscularly with 0.5 mL of either saline, APS (4 or 8 mg/kg of b...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.02.040
更新日期：2015-05-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.08.166
更新日期：2018-12-01 00:00:00
abstract：:Trace elements regulate many biological reactions in the body. Copper(II) is known as one of trace elements and capable of binding to proteins. Insulin is a blood glucose-lowering peptide hormone and it is secreted by the pancreatic β-cells. In this study, Cu(II)-insulin complexes were investigated by using ESI-MS met...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.01.150
更新日期：2018-06-01 00:00:00
abstract：:In the present study, efficient enzymatic methods were developed using a recombinant metagenomic lipase (LipR1) for the synthesis of corresponding esters by the transesterification of five different pharmaceutically important secondary alcohols. The recombinant lipase (specific activity=87m6U/mg) showed maximum conver...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.10.088
更新日期：2017-02-01 00:00:00
abstract：:Crocin, a water-soluble carotenoid in saffron, is a highly bioactive compound with limited use due to instability to pH variations, heat and oxidative stress, rapid absorption and low bioavailability. In this study, chitosan-alginate nanoparticles, considered as a new vehicle for crocin to improve properties in terms ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.02.095
更新日期：2017-06-01 00:00:00
