Regulation of inflammatory transcription factors by heat shock protein 70 in primary cultured astrocytes exposed to oxygen-glucose deprivation.


:Inflammation is an important event in ischemic injury. These immune responses begin with the expression of pro-inflammatory genes modulating transcription factors, such as nuclear factor-κB (NF-κB), activator protein-1 (AP-1), and signal transducers and activator of transcription-1 (STAT-1). The 70-kDa heat shock protein (Hsp70) can both induce and arrest inflammatory reactions and lead to improved neurological outcome in experimental brain injury and ischemia. Since Hsp70 are induced under heat stress, we investigated the link between Hsp70 neuroprotection and phosphorylation of inhibitor of κB (IκB), c-Jun N-terminal kinases (JNK) and p38 through co-immunoprecipitation and enzyme-linked immunosorbent assay (ELISA) assay. Transcription factors and pro-inflammatory genes were quantified by immunoblotting, electrophoretic-mobility shift assay and reverse transcription-polymerase chain reaction assays. The results showed that heat stress led to Hsp70 overexpression which rendered neuroprotection after ischemia-like injury. Overexpression Hsp70 also interrupts the phosphorylation of IκB, JNK and p38 and blunts DNA binding of their transcription factors (NF-κB, AP-1 and STAT-1), effectively downregulating the expression of pro-inflammatory genes in heat-pretreated astrocytes. Taken together, these results suggest that overexpression of Hsp70 may protect against brain ischemia via an anti-inflammatory mechanism by interrupting the phosphorylation of upstream of transcription factors.






Kim JY,Yenari MA,Lee JE




Has Abstract


2015-02-12 00:00:00












  • Electron microscopic investigation of the vestibular projection to the cat trochlear nuclei.

    abstract::Ultrastructural degeneration studies were carried out on the cat trochlear nucleus following lesion of the vestibulo-trochlear pathway in order to characterize the location and type of presynaptic endings involved in this pathway. Four types of boutons are found in the normal trochlear nucleus. Types I and II are larg...


    pub_type: 杂志文章


    authors: Bak IJ,Baker R,Choi WB,Precht W

    更新日期:1976-12-01 00:00:00

  • Substantia nigra and ventral tegmental area projections to cortex: topography and collateralization.

    abstract::The substantia nigra and ventral tegmental area of the rat were examined by retrograde transport methods to determine the topography and collateralization patterns of projections to cortex and certain subcortical sites. The topographical relationships between cells and their terminal fields were confirmed and clarifie...


    pub_type: 杂志文章


    authors: Loughlin SE,Fallon JH

    更新日期:1984-02-01 00:00:00

  • Simvastatin increases excitability in the hippocampus via a PI3 kinase-dependent mechanism.

    abstract::Simvastatin is an HMG-CoA reductase inhibitor commonly used in the clinic to treat hypercholesterolemia. In addition, simvastatin has been shown to cross the blood-brain barrier and pleiotropic effects of simvastatin have been reported including anti-inflammatory properties, enhancement of neurite outgrowth, and memor...


    pub_type: 杂志文章


    authors: Métais C,Hughes B,Herron CE

    更新日期:2015-04-16 00:00:00

  • GABAA and GABAB receptor-mediated inhibition of release of 5-hydroxytryptamine in the intermediate lobe of the rat pituitary gland.

    abstract::Isolated neurointermediate lobes or neural lobes of the rat pituitary gland attached to the pituitary stalk were incubated in vitro and the spontaneous or electrically (pituitary stalk stimulation, 5 Hz, 1,500 pulses) evoked release of 5-hydroxytryptamine was determined. The evoked release of 5-hydroxytryptamine from ...


    pub_type: 杂志文章


    authors: Racké K,Holzbauer M,Sharman DF,Cooper TR

    更新日期:1987-11-01 00:00:00

  • Targeting the motor end plates in the mouse hindlimb gives access to a greater number of spinal cord motor neurons: an approach to maximize retrograde transport.

    abstract::Lower motor neuron dysfunction is one of the most debilitating neurological conditions and, as such, significantly impacts on the quality of life of affected individuals. Within the last decade, the engineering of mouse models of lower motor neuron diseases has facilitated the development of new therapeutic scenarios ...


    pub_type: 杂志文章


    authors: Mohan R,Tosolini AP,Morris R

    更新日期:2014-08-22 00:00:00

  • Neuronal vulnerability of stroke-prone spontaneously hypertensive rats to ischemia and its prevention with antioxidants such as vitamin E.

    abstract::Stroke-prone spontaneously hypertensive rats (SHRSP/Izm) develop severe hypertension, and more than 95% of them die of cerebral stroke. Hypoxic stimulation followed by oxygen reperfusion induces neuronal damage in both normotensive Wistar Kyoto/Izm (WKY/Izm) and SHRSP/Izm rats, and the percentage of neurons that under...


    pub_type: 杂志文章,评审


    authors: Yamagata K,Tagami M,Yamori Y

    更新日期:2010-09-29 00:00:00

  • Nerve growth factor reduces apoptosis of axotomized retinal ganglion cells in the neonatal rat.

    abstract::It has recently been reported that the degeneration of retinal ganglion cells induced by transection of the optic nerve in the neonatal rat is due to an active process of apoptosis, as opposed to passive necrosis. Here we tested whether the administration of the trophic factor nerve growth factor could prevent the apo...


    pub_type: 杂志文章


    authors: Rabacchi SA,Ensini M,Bonfanti L,Gravina A,Maffei L

    更新日期:1994-12-01 00:00:00

  • Fatty acid amide hydrolase inhibition enhances the anti-allodynic actions of endocannabinoids in a model of acute pain adapted for the mouse.

    abstract::Cannabinoid ligands have been shown to be anti-nociceptive in animal models of acute and chronic pain by acting at the two known cannabinoid receptors, cannabinoid-1 receptor (CB-1) and cannabinoid-2 receptor (CB-2). A major concern with the use of cannabinoids for pain relief is that they activate receptors at sites ...


    pub_type: 杂志文章


    authors: Palmer JA,Higuera ES,Chang L,Chaplan SR

    更新日期:2008-07-17 00:00:00

  • Visual input regulates the expression of basic fibroblast growth factor and its receptor.

    abstract::Emerging evidence indicates that the expression of trophic factors in the brain is regulated in an activity-dependent manner, which suggests an involvement of trophic factors in events controlled by input activity. We have investigated the possibility that visual sensory input impacts the expression of basic fibroblas...


    pub_type: 杂志文章


    authors: Gómez-Pinilla F,Choi J,Ryba EA

    更新日期:1999-01-01 00:00:00

  • Agmatine selectively improves behavioural function in aged male Sprague-Dawley rats.

    abstract::L-arginine, a semi-essential amino acid, can be metabolized to form a number of bioactive molecules. Nitric oxide (NO), generated by NO synthase (NOS) from L-arginine, has been strongly implicated in the aging process. Agmatine, decarboxylated arginine, regulates the production of NO and other metabolites of L-arginin...


    pub_type: 杂志文章


    authors: Rushaidhi M,Collie ND,Zhang H,Liu P

    更新日期:2012-08-30 00:00:00

  • The Positional Logic of Sensory-Motor Reflex Circuit Assembly.

    abstract::Throughout his scientific career, Tom Jessell pioneered the spinal cord as a model system to study the molecular programs of neural specification, axon guidance, and connection specificity. His contributions to these fields and more broadly to that of developmental neuroscience will continue to inspire and define many...


    pub_type: 杂志文章,评审


    authors: Balaskas N,Ng D,Zampieri N

    更新日期:2020-12-01 00:00:00

  • Histogenesis at the level of the basal forebrain: the entopeduncular nucleus.

    abstract::This study shows that the neurons of the entopeduncular nucleus are derived from a longitudinal slab of isochronically generated neurons on day 11 and 15 h of gestation. Many neurons of this longitudinal slab which we have named the basal forebrain cell column, originate from an ependymal matrix closely associated wit...


    pub_type: 杂志文章


    authors: Marchand R,Lajoie L,Blanchet C

    更新日期:1986-03-01 00:00:00

  • Modulation of septo-hippocampal Theta activity by GABAA receptors: an experimental and computational approach.

    abstract::Theta frequency oscillation of the septo-hippocampal system has been considered as a prominent activity associated with cognitive function and affective processes. It is well documented that anxiolytic drugs diminish septo-hippocampal oscillatory Theta activity contributing to their either therapeutic or unwanted side...


    pub_type: 杂志文章


    authors: Hajós M,Hoffmann WE,Orbán G,Kiss T,Erdi P

    更新日期:2004-01-01 00:00:00

  • BDNF and learning: Evidence that instrumental training promotes learning within the spinal cord by up-regulating BDNF expression.

    abstract::We have previously shown that the spinal cord is capable of learning a sensorimotor task in the absence of supraspinal input. Given the action of brain-derived neurotrophic factor (BDNF) on hippocampal learning, the current studies examined the role of BDNF in spinal learning. BDNF is a strong synaptic facilitator and...


    pub_type: 杂志文章


    authors: Gómez-Pinilla F,Huie JR,Ying Z,Ferguson AR,Crown ED,Baumbauer KM,Edgerton VR,Grau JW

    更新日期:2007-09-21 00:00:00

  • Calcium currents in rat cerebellar Purkinje cells maintained in culture.

    abstract::Calcium permeabilities were examined in large cerebellar neurons maintained in culture, and morphologically identified as Purkinje cells. When cells were supplied with a Dulbecco Minimum Eagle's Medium with 10% horse serum added (5-10 days), somatic recordings revealed complex spikes and these were shown to be generat...


    pub_type: 杂志文章


    authors: Bossu JL,Dupont JL,Feltz A

    更新日期:1989-01-01 00:00:00

  • Age-dependent effects of environmental enrichment on brain networks and spatial memory in Wistar rats.

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    pub_type: 杂志文章


    authors: Sampedro-Piquero P,Begega A,Zancada-Menendez C,Cuesta M,Arias JL

    更新日期:2013-09-17 00:00:00

  • Characterization of calbindin-positive cones in primates.

    abstract::The aim of this study is to characterize calbindin-positive photoreceptors and their opsin content in the retina of nocturnal prosimians (Microcebus murinus), New World monkeys (Callithrix jacchus), Old World monkeys (Macaca fascicularis), and humans. To identify the calbindin and opsin content of cones, combined mult...


    pub_type: 杂志文章


    authors: Chiquet C,Dkhissi-Benyahya O,Chounlamountri N,Szel A,Degrip WJ,Cooper HM

    更新日期:2002-01-01 00:00:00

  • Basal forebrain cholinergic cell attachment and neurite outgrowth on organotypic slice cultures of hippocampal formation.

    abstract::Distributions of somata and neurites of cholinergic neurons were studied after seeding dissociated cells onto organotypic slice cultures. Slice cultures were made from hippocampal formation and adjacent cortical regions from rats or mice. Dissociated cell suspensions of basal forebrain tissue from rat or mouse fetuses...


    pub_type: 杂志文章


    authors: Tsai ES,Haraldson SJ,Baratta J,Lander AD,Yu J,Robertson RT

    更新日期:2002-01-01 00:00:00

  • Distinct neural mechanisms for repetition effects of visual objects.

    abstract::Repetition of visually common objects was examined in relation to prior intentional learning and memory status using a delayed match-to-sample task in humans. Both response time and two temporally separate event-related potential (ERP) components indexed repetition. The early repetition effect ( approximately 200-550 ...


    pub_type: 杂志文章


    authors: Guo C,Lawson AL,Jiang Y

    更新日期:2007-11-23 00:00:00

  • Contextual modulation of frequency tuning of neurons in the rat auditory cortex.

    abstract::In a natural acoustic environment, sound stimuli often occur in a contextual acoustic stream. The aim of the present study was to determine how the frequency tuning of auditory cortical neurons is affected by an acoustic context. A forward masking paradigm was used to determine the frequency receptive fields of rat au...


    pub_type: 杂志文章


    authors: Peng Y,Sun X,Zhang J

    更新日期:2010-09-01 00:00:00

  • Synergistic interactions of dopamine D1 and glutamate NMDA receptors in rat hippocampus and prefrontal cortex: involvement of ERK1/2 signaling.

    abstract::Interactions between dopamine and glutamate receptors are essential for the prefrontal cortical (PFC) and hippocampal cognitive functions. In order to understand the molecular basis of dopamine/glutamate interactions in rat PFC and hippocampus, we investigated (a) the effect of in vitro dopamine D1 receptor stimulatio...


    pub_type: 杂志文章


    authors: Sarantis K,Matsokis N,Angelatou F

    更新日期:2009-11-10 00:00:00

  • The role of the protein glycosylation state in the control of cellular transport of the amyloid beta precursor protein.

    abstract::The amyloid beta precursor protein can exist as both a membrane-bound and a secreted protein, with the former having the potential to generate the amyloid beta peptide present in the neuritic plaques which are characteristic of Alzheimer's disease. In this study, we have used a clone of the AtT20 mouse pituitary cell ...


    pub_type: 杂志文章


    authors: McFarlane I,Georgopoulou N,Coughlan CM,Gillian AM,Breen KC

    更新日期:1999-04-01 00:00:00

  • Frequency bands and spatiotemporal dynamics of beta burst stimulation induced afterdischarges in hippocampus in vivo.

    abstract::Temporal and spatial characteristics of hippocampal neuronal network activation are modified during epileptiform afterdischarges. We developed a beta burst stimulation protocol to investigate subregional variations and substrates of rhythmic population spike discharges in vivo in urethane anesthetized Wistar rat hippo...


    pub_type: 杂志文章


    authors: Mikkonen JE,Penttonen M

    更新日期:2005-01-01 00:00:00

  • A strategy of faster movements used by elderly humans to lift objects of increasing weight in ecological context.

    abstract::It is not known whether, during the course of aging, changes occur in the motor strategies used by the CNS for lifting objects of different weights. Here, we analyzed the kinematics of object-lifting in two different healthy groups (young and elderly people) plus one well-known deafferented patient (GL). The task was ...


    pub_type: 杂志文章


    authors: Hoellinger T,McIntyre J,Jami L,Hanneton S,Cheron G,Roby-Brami A

    更新日期:2017-08-15 00:00:00

  • Ontogeny of cholecystokinin-8 containing neuron system of the rat: an immunohistochemical analysis--II. Lower brain stem.

    abstract::The ontogeny of the cholecystokinin-8 neuron system in the lower brain stem of the rat was investigated by means of indirect immunofluorescence. Cholecystokinin-8-like immunoreactive structures first appeared in the primordium of the ventral tegmental area, ventral tegmental nucleus of Gudden and the area lateral to t...


    pub_type: 杂志文章


    authors: Kiyama H,Shiosaka S,Kubota Y,Cho HJ,Takagi H,Tateishi K,Hashimura E,Hamaoka T,Tohyama M

    更新日期:1983-12-01 00:00:00

  • Long-term potentiation during whole-cell recording in rat hippocampal slices.

    abstract::Factors involved in the production of long-term potentiation in the CA1 region of rat hippocampal slices were examined using whole-cell voltage clamp recordings. The pairing of postsynaptic membrane depolarization with tetanic stimulation produced a reliable long-lasting enhancement of synaptic currents provided that ...


    pub_type: 杂志文章


    authors: Kato K,Clifford DB,Zorumski CF

    更新日期:1993-03-01 00:00:00

  • Melatonin generates an outward potassium current in rat suprachiasmatic nucleus neurones in vitro independent of their circadian rhythm.

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    pub_type: 杂志文章


    authors: van den Top M,Buijs RM,Ruijter JM,Delagrange P,Spanswick D,Hermes ML

    更新日期:2001-01-01 00:00:00

  • Lactate Administration Reduces Brain Injury and Ameliorates Behavioral Outcomes Following Neonatal Hypoxia-Ischemia.

    abstract::Neonatal hypoxic-ischemic encephalopathy is a major cause of mortality and disability in newborns and the only standard approach for treating this condition is therapeutic hypothermia, which shows some limitations. Thus, putative neuroprotective agents have been tested in animal models. The present study evaluated the...


    pub_type: 杂志文章


    authors: Tassinari ID,Andrade MKG,da Rosa LA,Hoff MLM,Nunes RR,Vogt EL,Fabres RB,Sanches EF,Netto CA,Paz AH,de Fraga LS

    更新日期:2020-11-10 00:00:00

  • Functional GABA(B) receptors are expressed at the cone photoreceptor terminals in bullfrog retina.

    abstract::GABA(B) receptors at the cone terminals in bullfrog retina were characterized by immunocytochemical and whole-cell patch clamp techniques in retinal slice preparations. Somata, axons and synaptic terminals (pedicles) of cones were both GABA(B) receptor (GABA(B)R) 1 and GABA(B)R2 immunoreactive. Physiologically, barium...


    pub_type: 杂志文章


    authors: Liu J,Zhao JW,Du JL,Yang XL

    更新日期:2005-01-01 00:00:00

  • HMGB1 may act via RAGE to promote angiogenesis in the later phase after intracerebral hemorrhage.

    abstract::Following intracerebral hemorrhage (ICH), high-mobility group box 1 protein (HMGB1) may promote vascular remodeling. Whether HMGB1 supports angiogenesis after ICH is unclear, as are the receptors and downstream signaling pathway(s) involved. We used the rat model of collagenase-induced ICH to determine whether HMGB1 a...


    pub_type: 杂志文章


    authors: Lei C,Zhang S,Cao T,Tao W,Liu M,Wu B

    更新日期:2015-06-04 00:00:00