Abstract：
:Neurogenesis continues throughout adult life in the mammalian olfactory epithelium. This process is a dynamic state of proliferation, differentiation and cell death, probably regulated by autocrine and paracrine signals such as peptide growth factors. Previous investigations have demonstrated roles for some growth factors in olfactory neurogenesis in vitro, but the assay systems used make it difficult to be certain of their effects (proliferation, differentiation, enhanced survival) or their target cells. The present study investigated the effects of growth factors in cultures of purified olfactory epithelium comprising only basal cells and supporting cells in serum-free media. The advantage of this culture system is that proliferation, differentiation and survival of the basal cells and neurons can be examined separately. Under these conditions, three growth factors exerted well-defined effects: (i) fibroblast growth factor-2 stimulated proliferation of the globose basal cells; (ii) transforming growth factor-beta2 induced these cells to differentiate into neurons; and (iii) platelet-derived growth factor promoted survival of the differentiated neurons. We conclude that fibroblast growth factor-2, transforming growth factor-beta2 and platelet-derived growth factor act sequentially on precursor cells and immature neurons during neurogenesis in the adult olfactory epithelium.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Newman MP,Féron F,Mackay-Sim Adoi
10.1016/s0306-4522(00)00194-9keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
343-50issue
2eissn
0306-4522issn
1873-7544pii
S0306-4522(00)00194-9journal_volume
99pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract：:The distribution of the diadenosine tetraphosphate high-affinity binding sites has been studied in rat brain by an autoradiographic method using [3H]diadenosine tetraphosphate as the ligand. The binding characteristics are comparable to those described in studies performed on rat brain synaptosomes. White matter is de...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(96)00424-1
更新日期：1997-03-01 00:00:00
abstract：:We addressed the issue of the possible degradation of the aiming precision of a whole-body pointing task, when movement coordination is deranged by selective fatigue of the postural task component. The protocol involved continuous repetition (0.1 Hz frequency) of rapid whole-body pointing trials toward a target locate...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2005.12.060
更新日期：2006-01-01 00:00:00
abstract：:Dementia, especially Alzheimer's disease, is a rapidly increasing medical condition that presents with enormous challenge for treatment. It is characterized by impairment in memory and cognitive function often accompanied by changes in synaptic transmission and plasticity in relevant brain regions such as the hippocam...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2012.06.028
更新日期：2012-09-18 00:00:00
abstract：:Traumatic brain injury (TBI) is a major cause of morbidity and mortality world-wide and can result in persistent cognitive, sensory and behavioral dysfunction. Understanding the time course of TBI-induced pathology is essential to effective treatment outcomes. We induced TBI in rats using an impact acceleration method...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2016.08.018
更新日期：2016-10-15 00:00:00
abstract：BACKGROUND AND PURPOSE:Peptide analog of thymulin (PAT) has been shown to have anti-hyperalgesic and anti-inflammatory properties in animal models of inflammation. Recent reports suggest that the peripheral cholinergic system has an anti-inflammatory role mediated by α7-nicotinic acetylcholine receptor (α7-nAChR). Our ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2013.07.031
更新日期：2013-10-10 00:00:00
abstract：:White matter lesions are common when global cerebral ischemia (GCI) occurs in the elderly, and cause damage to neurological and psychological functions. Remyelination often fails because of the limited recruitment of oligodendrocyte progenitor cells (OPCs) to the demyelinated site or the inefficient differentiation of...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2020.05.011
更新日期：2020-07-01 00:00:00
abstract：:Oxytocin and vasopressin binding sites were localized and characterized by quantitative autoradiography on consecutive sections of Long-Evans rat forebrains and pituitary glands, incubated in the presence of 5 nM [3H]oxytocin or 5 nM [3H]vasopressin. In the forebrain, two types of neurohypophysial hormone binding site...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(88)90143-1
更新日期：1988-07-01 00:00:00
abstract：:The inhibitory neurotransmitter GABA plays an important role in regulating the activity of magnocellular oxytocin and vasopressin neurons located in the supraoptic and paraventricular nuclei through occupancy of GABAA receptors. However, the GABAA receptor is a hetero-oligomeric protein comprised of different subunits...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(94)00402-q
更新日期：1995-02-01 00:00:00
abstract：:The aim of this study was to examine the role of metabotropic glutamate receptors (mGluR) in the generation of oscillatory field activity at theta frequency (4-12 Hz) in the medial septal slice prepared from rat brain. Bath application of mGluR agonists and antagonists showed that activation of mGluR1-type receptors p...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2011.01.004
更新日期：2011-03-17 00:00:00
abstract：:Nervous system formation integrates control of cellular proliferation and differentiation and is mediated by multipotent neural progenitor cells that become progressively restricted in their developmental potential before they give rise to differentiated neurons and glial cells. Evidence from different experimental sy...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2007.03.050
更新日期：2007-06-29 00:00:00
abstract：:The vertebrate blood-brain barrier (BBB) creates an obstacle for central nervous system-related drug delivery. Claudin-5 (Cldn5), expressed in large quantities in BBB, plays a vital role in restricting BBB permeability. The C-terminal domain of Clostridium perfringens enterotoxin (cCPE) has been verified as binding to...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2016.04.013
更新日期：2016-07-07 00:00:00
abstract：:In the present study we examined the effects of normal aging in the hippocampus and cerebellum, as well as behaviors associated with these substrates. A total of 67 CB6F1 hybrid mice were tested at one of five ages (4, 8, 12, 18 or 25 months) on the context pre-exposure facilitation effect (CPFE) modification of fear ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2013.06.002
更新日期：2013-09-05 00:00:00
abstract：:The pharmacological features of putative nicotinic acetylcholine receptor sites involved in the release of [3H]noradrenaline were assessed in rat hippocampus. The effect of nicotinic agonists to induce [3H]noradrenaline release was examined in superfused slices. The nicotinic agonists (-)-epibatidine, (+)-anatoxin-a, ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(96)00425-3
更新日期：1997-03-01 00:00:00
abstract：:The paraventricular nucleus of the hypothalamus is increasingly being viewed as an important site for cardiovascular integration because of its connections to regions in the brain and spinal cord which are known to be important in cardiovascular control. Like the vasomotor neurons of the rostral ventrolateral medulla,...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(98)00255-3
更新日期：1999-01-01 00:00:00
abstract：:A variety of neurological diseases are characterized by disturbances of the blood-brain barrier (BBB) and its transporters. We recently introduced fibroblast growth factor treated cortical organotypic slice cultures of mice as a model for in vitro studies of the blood-brain barrier and have now further characterized t...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2010.06.073
更新日期：2010-09-29 00:00:00
abstract：:This report presents the major structural features of neurons and their afferent input in nucleus magnocellularis, the avian homologue of the mammalian anteroventral cochlear nucleus. Results of light-microscope observations, as seen in Golgi, Nissl, and normal fiber preparations, as well as ultrastructural morphology...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(82)90045-8
更新日期：1982-04-01 00:00:00
abstract：:Levels of the pro-inflammatory cytokine interleukin-1beta are known to be elevated in patients with chronic disorders such as Alzheimer's disease. We have investigated the effects of interleukin-1beta on long-term potentiation and N-methyl-D-aspartate receptor-mediated field potentials in the rat dentate gyrus in vitr...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(99)00100-1
更新日期：1999-01-01 00:00:00
abstract：:The regulation of D₃ receptor has not been well documented in diffuse Lewy body disease (DLBD). In this study, a novel D₃-preferring radioligand [(3)H]WC-10 and a D₂-preferring radioligand [(3)H]raclopride were used and the absolute densities of the dopamine D₃ and D₂ receptors were determined in the striatal regions ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2013.05.048
更新日期：2013-09-17 00:00:00
abstract：:In the present study we have examined the topographic and temporal patterns of expression of the limbic system associated membrane protein by light and electron microscopic immunocytochemistry in organotypic cultures of the rat brain. The regional, cellular and subcellular distribution of staining in young cultures wa...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(89)90192-9
更新日期：1989-01-01 00:00:00
abstract：:Brain microvascular endothelial cell (BMEC) injury induced by ischemia-reperfusion (I/R) is the initial stage of blood-brain barrier (BBB) disruption, which results in a poor prognosis in ischemic stroke patients. Autophagy has been shown to have protective effects on BMECs against cerebral ischemic insults. However, ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2017.04.017
更新日期：2017-06-23 00:00:00
abstract：:In order to determine which types of spinal neuron produce c-fos in response to noxious stimulation, we have combined pre-embedding detection of c-fos-like immunoreactivity with post-embedding immunocytochemistry using antibodies against GABA and glycine, 2 h after subcutaneous injection of formalin into a hindpaw of ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(94)90525-8
更新日期：1994-12-01 00:00:00
abstract：:The aim of the present study was to investigate the existence and distribution of neuropeptide Y receptor subtypes in various regions of the normal human brain using the peptide YY derivative receptor probes, [125I][Leu31,Pro34]polypeptide YY/Y1 and [125I]polypeptide YY(3-36)/Y2, in addition to the non-selective ligan...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(96)00639-2
更新日期：1997-07-01 00:00:00
abstract：:Cytochrome oxidase is the terminal enzyme in the mitochondrial respiratory chain engaged in oxidative metabolism and energy production. In mammals, the holoenzyme is composed of 13 subunits encoded by both nuclear and mitochondrial genomes. The goal of the present study was to compare the effect of afferent impulse bl...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(95)00043-i
更新日期：1995-07-01 00:00:00
abstract：:Stimulus-induced pattern of bioelectric activity in human neocortical tissue was investigated by use of the voltage sensitive dye RH795 and a fast optical recording system. During control conditions stimulation of layer I evoked activity predominantly in supragranular layers showing a spatial extent of up to 3000 micr...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(03)00530-x
更新日期：2003-01-01 00:00:00
abstract：:To evaluate the potential for lipofectin-mediated central nervous system gene transfer, the plasmid coding for cholecystokinin was administered intracerebroventricularly to rats, which have congenital audiogenic seizures and high responses to peripheral electric stimulation-induced analgesia. Previous studies had show...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(96)00420-4
更新日期：1997-03-01 00:00:00
abstract：:Eider duck (Somateria mollissima) cerebellar neurons are highly tolerant toward hypoxia in vitro, which in part is due to a hypoxia-induced depression of their spontaneous activity. We have studied whether this response involves ATP-sensitive potassium (KATP) channels, which are known to be involved in the hypoxic/isc...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2014.10.046
更新日期：2015-01-22 00:00:00
abstract：:Interleukin-1beta is a relatively small and abundant polypeptide that plays diverse roles in the central nervous system. In the present study, patterns of interleukin-1beta expression were observed in the olfactory bulbs of rats that had either undergone unilateral closure of the external naris or sham surgery on post...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(99)00093-7
更新日期：1999-01-01 00:00:00
abstract：:I.v. injection of secretin activates neurons in brain areas controlling autonomic function and emotion. Peripheral administration of secretin inhibits gastric functions through a central mechanism that is mediated by vagal dependent pathways. We investigated whether the vagus nerve is involved in i.p. injection of sec...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.06.027
更新日期：2004-01-01 00:00:00
abstract：:Dopamine/cAMP signaling has been reported to mediate behavioral responses related to drug addiction. It also modulates the plasticity and firing properties of medium spiny neurons (MSNs) in the nucleus accumbens (NAc), although the effects of cAMP signaling on the resting membrane potential (RMP) of MSNs has not been ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2010.02.075
更新日期：2010-05-19 00:00:00
abstract：:Field and intracellular potentials were recorded from CA1 pyramidal stratum in submerged slices (at 33 degrees). During "normal" oxygenation (95% O2 + 5% CO2), tonic depression of population spikes and field excitatory postsynaptic potentials by endogenous adenosine was demonstrated by (i) the marked enhancement by th...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(94)90545-2
更新日期：1994-11-01 00:00:00
