当前位置： SCI文献检索 > NEUROSCIENCE期刊下所有文献 > Lipofectin-facilitated transfer of cholecystokinin gene corrects behavioral abnormalities of rats with audiogenic seizures.

Lipofectin-facilitated transfer of cholecystokinin gene corrects behavioral abnormalities of rats with audiogenic seizures.

Abstract：

:To evaluate the potential for lipofectin-mediated central nervous system gene transfer, the plasmid coding for cholecystokinin was administered intracerebroventricularly to rats, which have congenital audiogenic seizures and high responses to peripheral electric stimulation-induced analgesia. Previous studies had shown that low brain cholecystokinin levels may be the neurochemical variable of rat's audiogenic seizure and high responses to the analgesia because cholecystokinin is an anticonvulsant and anti-opioid neuropeptide. Gene transfer of cholecystokinin corrected the increased susceptibility to audiogenic seizures and the high responses to analgesia for about one week. Similar administration of plasmid expressing beta-galactosidase indicated that the vector mainly transfected ependymal cells lining the ventricle and pia mater cells. The increased cholecystokinin messenger RNA and immunoreactivity in the hippocampus following stereotactic intrahippocampal administration of cholecystokinin plasmid was also demonstrated with in situ hybridization and immunohistochemistry techniques. These results suggest that lipofectin-mediated gene transfer will be useful for studies of brain function, the modification of behavior and gene therapy for central nervous system disorders.

journal_name

Neuroscience

journal_title

Neuroscience

authors

Zhang LX,Li XL,Smith MA,Post RM,Han JS

doi

10.1016/s0306-4522(96)00420-4

subject

Has Abstract

pub_date

1997-03-01 00:00:00

pages

15-22

issue

1

eissn

0306-4522

issn

1873-7544

pii

S0306-4522(96)00420-4

journal_volume

77

pub_type

杂志文章

相关文献

NEUROSCIENCE文献大全
  • Calcium-induced long-term potentiation in the hippocampus.

    abstract：:The effect of a transient increase in extracellular calcium concentration on the Schaffer collateral-commissural evoked excitatory postsynaptic potential and population spike responses of CAI pyramidal neurons was investigated using the rat in vitro hippocampal slice preparation. Brief exposure of slices (5-10 min) to...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(82)90254-8

    authors： Turner RW,Baimbridge KG,Miller JJ

    更新日期：1982-06-01 00:00:00

  • Electron microscopic investigation of the vestibular projection to the cat trochlear nuclei.

    abstract：:Ultrastructural degeneration studies were carried out on the cat trochlear nucleus following lesion of the vestibulo-trochlear pathway in order to characterize the location and type of presynaptic endings involved in this pathway. Four types of boutons are found in the normal trochlear nucleus. Types I and II are larg...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(76)90099-3

    authors： Bak IJ,Baker R,Choi WB,Precht W

    更新日期：1976-12-01 00:00:00

  • P2X₃ and TRPV1 functionally interact and mediate sensitization of trigeminal sensory neurons.

    abstract：:Musculoskeletal pain conditions, particularly those associated with temporomandibular disorders (TMD) affect a large percentage of the population. Identifying mechanisms underlying hyperalgesia could contribute to the development of new treatment strategies for the management of TMD and other muscle pain conditions. I...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2012.11.015

    authors： Saloman JL,Chung MK,Ro JY

    更新日期：2013-03-01 00:00:00

  • Hyperbaric oxygen treatment attenuated the decrease in regional glucose metabolism of rats subjected to focal cerebral ischemia: a high resolution positron emission tomography study.

    abstract：:Cerebral hypoxia may be the main component of cell damage caused by ischemia. Previous studies demonstrated a neuroprotective effect of early hyperbaric oxygen (HBO) treatment in various animal models of focal cerebral ischemia. Neuropathologic study showed that exposure of HBO may prevent cell death in ischemic corte...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2007.01.046

    authors： Lou M,Zhang H,Wang J,Wen SQ,Tang ZQ,Chen YZ,Yan WQ,Ding MP

    更新日期：2007-05-11 00:00:00

  • Cholinergic neurons of the adult rat striatum are immunoreactive for glutamatergic N-methyl-d-aspartate 2D but not N-methyl-d-aspartate 2C receptor subunits.

    abstract：:Cholinergic neurons of the striatum play a crucial role in controlling output from this region. Their firing is under the control of a relatively limited glutamatergic input, deriving principally from the thalamus. Glutamate transmission is effected via three major subtypes of receptors, including those with affinity ...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2007.09.035

    authors： Bloomfield C,O'Donnell P,French SJ,Totterdell S

    更新日期：2007-12-12 00:00:00

  • Substance P modifies the 6-hydroxydopamine induced alteration of postnatal development of central noradrenaline neurons.

    abstract：:Systemic treatment of new-born rats with the catecholamine neurotoxin 6-hydroxydopamine leads to a permanent and selective alteration of the postnatal development of the central noradrenaline neurons, in particular of the locus coeruleus system. The changes involve a pronounced and permanent degeneration of distant ne...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(82)90113-0

    authors： Jonsson G,Hallman H

    更新日期：1982-01-01 00:00:00

  • Association of gephyrin and glycine receptors in the human brainstem and spinal cord: an immunohistochemical analysis.

    abstract：:Gephyrin is a postsynaptic clustering molecule that forms a protein scaffold to anchor inhibitory neurotransmitter receptors at the postsynaptic membrane of neurons. Gephyrin was first identified as a protein component of the glycine receptor complex and is also colocalized with several GABAA receptor subunits in rode...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(03)00543-8

    authors： Baer K,Waldvogel HJ,During MJ,Snell RG,Faull RL,Rees MI

    更新日期：2003-01-01 00:00:00

  • Immunohistochemical localization of mu-opioid receptors in rat brain using antibodies generated against a peptide sequence present in a purified mu-opioid binding protein.

    abstract：:Light-microscope visualization in rat brain of a pattern of distribution of immunoreactivity, which included immunolabeled perikarya and beaded processes, was achieved using an immunoaffinity purified polyclonal antibody, Ab165, which recognizes the amino acid sequence, IRNLRQDRSKYY, found in the mu-opioid binding pro...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(94)90480-4

    authors： Hiller JM,Zhang Y,Bing G,Gioannini TL,Stone EA,Simon EJ

    更新日期：1994-10-01 00:00:00

  • Development of the suprachiasmatic nucleus in rats during ontogenesis: serotonin-immunopositive fibers.

    abstract：:The innervation of the suprachiasmatic nucleus by serotoninergic fibers has been studied in rats from the 22nd embryonic until the 21st postnatal day. The serotoninergic fibers were detected with the pre-embedding immunocytochemical technique at the electron-microscopic level using antibodies to serotonin. Serotonin-i...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(94)90163-5

    authors： Ugrumov MV,Popov AP,Vladimirov SV,Kasmambetova S,Novodjilova AP,Tramu G

    更新日期：1994-01-01 00:00:00

  • Cell density increases Bcl-2 and Bcl-x expression in addition to survival of cultured cerebellar granule neurons.

    abstract：:The proto-oncogene bcl-2 and its family members, bcl-x and bax are recognized as major regulators of cell death and survival. Although Bcl-2 and Bcl-x are expressed in brain, little is known how they are regulated in neurons. Here we have studied the expression of bcl-2, bcl-xL and bax mRNA in rat cerebellar granule n...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(96)00204-7

    authors： Ohga Y,Zirrgiebel U,Hamnér S,Michaelidis TM,Cooper J,Thoenen H,Lindholm D

    更新日期：1996-08-01 00:00:00

  • The distribution of Alz-50 immunoreactivity in the normal human brain.

    abstract：:Alz-50 is a monoclonal antibody that recognizes normal tau proteins as well as phosphorylated tau proteins that are associated with paired helical filaments in Alzheimer's disease. To establish an accurate baseline for future pathological studies, we examined the distribution of Alz-50 immunoreactivity in normal human...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(93)90567-y

    authors： Rye DB,Leverenz J,Greenberg SG,Davies P,Saper CB

    更新日期：1993-09-01 00:00:00

  • Unilateral 6-hydroxydopamine lesion of dopamine neurons and subchronic L-DOPA administration in the adult rat alters the expression of the vesicular GABA transporter in different subsets of striatal neurons and in the substantia nigra, pars reticulata.

    abstract：:The loss of dopamine neurons combined or not with the subsequent administration of L-DOPA in patients with Parkinson's disease or in experimental models of the disease results in altered GABAergic signaling throughout the basal ganglia, including the striatum and the substantia nigra, pars reticulata. However, the mol...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2006.12.001

    authors： Wang H,Katz J,Dagostino P,Soghomonian JJ

    更新日期：2007-03-16 00:00:00

  • Histamine H2 receptor mediates postsynaptic excitation and presynaptic inhibition in submucous plexus neurons of the guinea-pig.

    abstract：:Intracellular recordings were made from submucous plexus neurons of the guinea-pig cecum maintained in vitro. Histamine (0.3-10 microM) produced a dose-dependent membrane depolarization (congruent to 13 mV with 3 microM) in about 28% of the cells tested; most of these cells showed a prominent calcium-activated potassi...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(89)90018-3

    authors： Tokimasa T,Akasu T

    更新日期：1989-01-01 00:00:00

  • Delayed axonal degeneration in slow Wallerian degeneration mutant mice detected using diffusion tensor imaging.

    abstract：:Previous studies have shown the feasibility of using diffusion tensor imaging (DTI) as a noninvasive imaging modality to evaluate neurodegeneration in humans and animals. The axial and radial diffusivities derived from DTI were demonstrated to be sensitive markers for axonal and myelin damage, respectively. This study...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2011.09.042

    authors： Xie M,Wang Q,Wu TH,Song SK,Sun SW

    更新日期：2011-12-01 00:00:00

  • Activity of deep dorsal horn neurons in the anaesthetized rat during hyperalgesia of the hindpaw induced by ultraviolet irradiation.

    abstract：:Thermal hyperalgesia was induced by UV irradiation of the glabrous skin of the hindpaw of adult female Sprague-Dawley rats. We have recorded single cell activity and studied excitability changes in wide dynamic range neurons in the lumbar spinal segments during the early phase (days 1-3) and late phase (days 5-7) of t...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(93)90118-y

    authors： Urban L,Perkins MN,Campbell E,Dray A

    更新日期：1993-11-01 00:00:00

  • Hypothermic preconditioning reduces Purkinje cell death possibly by preventing the over-expression of inducible nitric oxide synthase in rat cerebellar slices after an in vitro simulated ischemia.

    abstract：:We showed that hypothermic preconditioning (HPC) increased survival of Purkinje neurons in rat cerebellar slices after oxygen-glucose deprivation (OGD). HPC also reduced the OGD-increased expression of high mobility group I (Y) proteins, a transcription factor that can enhance inducible nitric oxide synthase (iNOS) ex...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2006.06.053

    authors： Yuan HB,Huang Y,Zheng S,Zuo Z

    更新日期：2006-10-13 00:00:00

  • Differential expression of striatal synaptotagmin mRNA isoforms in hemiparkinsonian rats.

    abstract：:Synaptotagmins (Syts) constitute a multi-gene family of 15 putative membrane trafficking proteins. The expression of some of the Syts in the brain might be dopaminergically controlled and thus affected by dopamine depletion in Parkinson's disease. We used hemiparkinsonian rats to investigate the effects of chronic str...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2005.05.050

    authors： Glavan G,Zivin M

    更新日期：2005-01-01 00:00:00

  • Study on Urine Metabolic Profile of Aβ25-35-Induced Alzheimer's Disease Using UHPLC-Q-TOF-MS.

    abstract：:Alzheimer's disease (AD) is a progressive neurodegenerative disorder, with no effective method for its treatment so far. The pathogenesis of AD has been reported, but the endogenous metabolic profile and disease-related biomarkers are still not clear. To better understand AD, an AD model induced by injecting β-amyloid...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2018.10.001

    authors： Liu Y,Wei M,Yue K,Hu M,Li S,Men L,Pi Z,Liu Z,Liu Z

    更新日期：2018-12-01 00:00:00

  • Axon diameter distributions across the monkey's optic nerve.

    abstract：:The distribution of axons according to diameter has been examined in the optic nerve of old world monkeys. Axon diameters were measured from electron micrographs, and histograms were constructed at regular intervals across a section through the optic nerve to reveal the local axon diameter distribution. The total axon...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(88)90231-x

    authors： Reese BE,Ho KY

    更新日期：1988-10-01 00:00:00

  • In vitro autoradiography of ionotropic glutamate receptors in hippocampus and striatum of aged Long-Evans rats: relationship to spatial learning.

    abstract：:Using in vitro autoradiography, we investigated [3H] alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate, [3H]kainate and [3H]N-methyl-D-aspartate binding in two forebrain regions, the hippocampus and striatum, of young (four months of age) and aged (24-25 months of age) Long-Evans rats that had previously been teste...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(96)00147-9

    authors： Nicolle MM,Bizon JL,Gallagher M

    更新日期：1996-10-01 00:00:00

  • The Sport Expert's Attention Superiority on Skill-related Scene Dynamic by the Activation of left Medial Frontal Gyrus: An ERP and LORETA Study.

    abstract：:Extensive studies have shown that a sports expert is superior to a sports novice in visually perceptual-cognitive processes of sports scene information, however the attentional and neural basis of it has not been thoroughly explored. The present study examined whether a sport expert has the attentional superiority on ...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2018.02.043

    authors： He M,Qi C,Lu Y,Song A,Hayat SZ,Xu X

    更新日期：2018-05-21 00:00:00

  • Neural xenotransplantation: reconstruction of neuronal circuitry across species barriers.

    abstract：:Selective replacement of degenerated neurons in the adult brain with allogeneic fetal neuroblasts is a promising therapeutic modality for human neurodegenerative diseases, but is confounded with practical and potential ethical problems. To evaluate the potential of xenogeneic donors as a cell source for neural transpl...

    journal_title：Neuroscience

    pub_type： 杂志文章,meta分析

    doi：10.1016/0306-4522(94)90338-7

    authors： Pakzaban P,Isacson O

    更新日期：1994-10-01 00:00:00

  • Patterns of the UP-Down state in normal and epileptic mice.

    abstract：:Goal of this manuscript is to investigate whether changes that exist in epileptic brain generating spontaneous seizures are reflected in the pattern of the UP-Down state (UDS) recorded from the neocortex and dentate gyrus. Experiments were carried out on naive and epileptic mice under urethane anesthesia. Local field ...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2012.08.059

    authors： Bragin A,Benassi SK,Engel J Jr

    更新日期：2012-12-06 00:00:00

  • Unilateral fetal mesencephalic grafts in intact rats reduce amphetamine-induced dopamine release in both striata. An in vivo voltammetric study.

    abstract：:This study investigated amphetamine-induced striatal dopamine release after intraventricular unilateral fetal mesencephalic grafts in otherwise intact rats. Dopamine was monitored in vivo by differential pulse voltammetry. In grafted animals, amphetamine-induced dopamine release was decreased compared to sham-grafted,...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(97)00236-4

    authors： Marburger A,Reum T,Drandarevski N,Morgenstern R

    更新日期：1997-12-01 00:00:00

  • Increased vulnerability of septal cholinergic neurons to partial loss of target neurons in aged rats.

    abstract：:To investigate whether the ageing process might affect neuron-target interactions which influence the phenotype of septal cholinergic neurons, we compared the response of these neurons to partial loss of target tissue in young adult and aged animals. Groups of young adult (four to six months) or aged (24-33 months) ma...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(96)00240-0

    authors： Cooper JD,Sofroniew MV

    更新日期：1996-11-01 00:00:00

  • Cholinergic regulation of striatal Nova mRNAs.

    abstract：:Alternative splicing is an important mechanism for expanding proteome diversity from a limited number of genes, especially in higher vertebrates. Brain-specific splicing factors play an important role in establishing specific patterns of alternative splicing in the brain and thereby contribute to its complex architect...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2010.05.013

    authors： Jelen N,Ule J,Zivin M

    更新日期：2010-08-25 00:00:00

  • Distribution of the C-terminal glycopeptide of the vasopressin prohormone in rat brain: an immunocytochemical study.

    abstract：:The distribution of the C-terminal glycopeptide of the vasopressin prohormone was mapped in rat brain by an immunocytochemical method using antibodies to the sheep glycopeptide. The antibodies did not react with vasopressin, oxytocin or their related neurophysins. Stained neural perikarya were observed in the hypothal...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(87)90046-7

    authors： Dubois-Dauphin M,Zakarian S

    更新日期：1987-06-01 00:00:00

  • In vivo dynamics and distribution of intracerebroventricularly administered gadodiamide, visualized by magnetic resonance imaging.

    abstract：:Direct injections into the cerebroventricles have been extensively utilized in neurophysiological studies. Mapping the distribution of injectate after intracerebroventricular injection has been made only by post mortem analysis, and the dynamic distribution of injectate within the brain has not been well characterized...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(98)00519-3

    authors： Bui JD,Nammari DR,Buckley DL,Inglis BA,Silver XS,Mareci TH,Phillips MI

    更新日期：1999-03-01 00:00:00

  • Astroglial expression of ceramide in Alzheimer's disease brains: a role during neuronal apoptosis.

    abstract：:Accumulating evidences indicate that ceramide is closely involved in apoptotic cell death in neurodegenerative disorders and aging. We examined ceramide levels in the cerebrospinal fluid (CSF) or brain tissues from patients with neurodegenerative disorders and the mechanism of how intra- and extracellular ceramide was...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2004.08.056

    authors： Satoi H,Tomimoto H,Ohtani R,Kitano T,Kondo T,Watanabe M,Oka N,Akiguchi I,Furuya S,Hirabayashi Y,Okazaki T

    更新日期：2005-01-01 00:00:00

  • Altered mitogen-activated protein kinase signaling, tau hyperphosphorylation and mild spatial learning dysfunction in transgenic rats expressing the beta-amyloid peptide intracellularly in hippocampal and cortical neurons.

    abstract：:The pathological significance of intracellular Abeta accumulation in vivo is not yet fully understood. To address this, we have studied transgenic rats expressing Alzheimer's-related transgenes that accumulate Abeta intraneuronally in the cerebral and hippocampal cortices but do not develop extracellular amyloid plaqu...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2004.07.036

    authors： Echeverria V,Ducatenzeiler A,Dowd E,Jänne J,Grant SM,Szyf M,Wandosell F,Avila J,Grimm H,Dunnett SB,Hartmann T,Alhonen L,Cuello AC

    更新日期：2004-01-01 00:00:00