Abstract:
:PACAP1-38, a ubiquitous and multifunctional regulator has been in the focus of neurotoxicity research due to its impressive neuroprotective potential. Although the literature extensively demonstrated its repressive effect on the apoptotic machinery in neurodegenerative models, there is a striking absence of analysis on its role in normal development. We performed quantitative analyses on caspase activity in developing retina upon 100, 50, 25 or 1 pmol intravitreal PACAP1-38 injection from postnatal day 1 (P1) through P7 in Wistar rats. Retinas were harvested at 6, 12, 18, 24 or 48 h post-injection. Apoptotic activity was revealed using fluorescent caspase 3/7 enzyme assay, western blots and TUNEL assay. Unexpectedly, we found that 100 pmol PACAP1-38 increased the activity of caspase 3/7 at P1 and P5 whereas it had no effect at P7. At P3, as a biphasic effect, PACAP1-38 repressed active caspase 3/7 at 18 h post-injection while increased their activity in 24 h post-injection. Amounts, smaller than 100 pmol, could not inhibit apoptosis whereas 50, 25 or 1 pmol PACAP1-38 could evoke significant elevation in caspase 3/7 activity. TUNEL-positive cells appeared in the proximal part of inner nuclear as well as ganglion cell layers in response to PACAP1-38 treatment. The fundamental novelty of these results is that PACAP1-38 induces apoptosis during early postnatal retinogenesis. The dose as well as stage-dependent response suggests that PACAP1-38 has a Janus face in apoptosis regulation. It not only inhibits development-related apoptosis, but as a long-term effect, facilitates it.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Nyisztor Z,Denes V,Kovacs-Valasek A,Hideg O,Berta G,Gabriel Rdoi
10.1016/j.neuroscience.2018.06.008subject
Has Abstractpub_date
2018-08-10 00:00:00pages
59-66eissn
0306-4522issn
1873-7544pii
S0306-4522(18)30419-6journal_volume
385pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::We have examined by immunoassay and immunohistochemistry, the expression of nerve growth factor in the rat hippocampus, one to 28 days after transient forebrain ischemia. In the CA1 area, the overall level of nerve growth factor expression remained constant over the first three days of reperfusion while it increased b...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00612-5
更新日期:1999-01-01 00:00:00
abstract::Hyperpolarization-activated, cyclic nucleotide-modulated (HCN) channels contribute to rhythmic spontaneous activity in the heart and CNS. Ectopic spontaneous neuronal activity has been implicated in the development and maintenance of acute and chronic hyperalgesia, allodynia and spontaneous pain. Previously, we docume...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.10.048
更新日期:2007-02-23 00:00:00
abstract::Microglia play important roles in the pathogenesis of Alzheimer's disease (AD), in part, by affecting the clearance of amyloid-β (Aβ) peptides. Most studies, however, used synthetic soluble Aβ (sAβ) at higher concentrations. The exact mechanisms underlying microglia-mediated clearance of physiological sAβ at very low ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.07.020
更新日期:2020-09-01 00:00:00
abstract::Besides formation of neurofibrillary tangles and neuron loss, the Alzheimer's disease brain is characterized by neuritic plaques consisting of beta-amyloid peptide deposits and impaired neurotransmission. The proteins Munc18a, Munc18-interacting protein 1 and Munc18-interacting protein 2 mediate exocytosis and decreas...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.11.017
更新日期:2006-01-01 00:00:00
abstract::We investigated the role of cyclic-AMP response element binding protein signaling in the induction of the immediate-early gene c-fos by baroreceptor activation in neurons of the nucleus tractus solitarii of anesthetized rats. Activation of the arterial baroreceptors with sustained hypertension significantly increased ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00273-5
更新日期:1999-01-01 00:00:00
abstract::Properties of excitatory synaptic responses in fast-spiking interneurons (FSIs) and pyramidal neurons (PNs) are different; however, the mechanisms and determinants of this diversity have not been fully investigated. In the present study, voltage-clamp recording of miniature excitatory post-synaptic currents (mEPSCs) w...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.06.034
更新日期:2015-08-20 00:00:00
abstract::Semi-quantitative immunocytochemistry was used to investigate the levels of cyclase response element-binding protein, phosphorylated cyclase response element-binding protein, Fos and Fos-related antigen immunoreactivity in the striatum of rats after acute or repeated amphetamine administration. Rats were perfused 20 m...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00274-m
更新日期:1995-11-01 00:00:00
abstract::Cultured neurons offer many advantages over a slice preparation for whole-cell patch-clamp studies, such as better control over the environment and space clamp control. However, heterogeneous cultures of neurons present problems in distinguishing the cell type from which recordings are made. The present study uses cor...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00153-0
更新日期:1999-01-01 00:00:00
abstract::Mutations in γ-aminobutyric acid A receptor (GABAA) subunits and sodium channel genes, especially GABRG2 and SCN1A, have been reported to be associated with febrile seizures (FS) and genetic epilepsy with febrile seizures plus (GEFS+). GEFS+ is a well-known family of epileptic syndrome with autosomal dominant inherita...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.04.049
更新日期:2020-07-01 00:00:00
abstract::The role of the cerebral cortex in maintaining human standing balance remains unclear. Beta corticomuscular coherence (CMC) provides a measure of communication between the sensory-motor cortex and muscle, but past literature has not demonstrated significant beta CMC during human stance. This study evaluated the effect...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.04.009
更新日期:2015-07-09 00:00:00
abstract::Exercise reduces the risk of developing a number of neurological disorders and increases the efficiency of cellular energy production. However, overly strenuous exercise produces oxidative stress. Proper oxygenation is crucial for the health of all tissues, and tight regulation of cellular oxygen is critical to balanc...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.03.015
更新日期:2015-06-04 00:00:00
abstract::The enkephalin-like immunoreactivity in the retina of chicks has been studied using immunohistochemical and radioimmunoassay techniques. The histochemical experiments showed that the immunoreactivity was confined to a subpopulation of amacrine cells in the inner nuclear layer which projected processes into sublaminae ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(84)90272-0
更新日期:1984-09-01 00:00:00
abstract::Sensory experience has a profound effect on neocortical neurons. Passive stimulation of whiskers or sensory deprivation from whiskers can induce long-lasting changes in neuronal responses or modify the receptive field in adult animals. We recorded barrel cortical neurons in urethane-anesthetized rats in layers 2/3 or ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.09.057
更新日期:2014-12-05 00:00:00
abstract::Apart from its role in regulating calcium there is growing evidence that vitamin D is a neuroactive steroid capable of regulating multiple pathways important for both brain development and mature brain function. Vitamin D induces its genomic effects through its nuclear receptor the vitamin D receptor (VDR). Although t...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.02.042
更新日期:2014-05-30 00:00:00
abstract::Intracellular recordings were made from neurones in myenteric ganglia of the guinea-pig ileum in vitro. Synaptic potentials were evoked by electrically stimulating presynaptic fibres as they entered the ganglion, using a small focal electrode. Slow synaptic depolarizations (excitatory postsynaptic potentials) were evo...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(85)90317-3
更新日期:1985-02-01 00:00:00
abstract::Apolipoprotein E isoforms may have differential effects on a number of pathological processes underlying Alzheimer's disease. Recent studies suggest that the amount, rather than the type, of apolipoprotein E may also be an important determinant for Alzheimer's disease. Therefore, understanding the regulated synthesis ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00224-x
更新日期:2001-01-01 00:00:00
abstract::The effects of excitatory amino acid antagonists on extracellular field potentials in the olfactory bulb produced by lateral olfactory tract stimulation were analysed in vivo. The compounds tested D-2-amino-5-phosphonovalerate, L-(+)2-amino-4-phosphonobutyrate, gamma-D-glutamylglycine, L-glutamic acid diethylester and...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(86)90020-5
更新日期:1986-09-01 00:00:00
abstract::The increase in excitatory outflow from the medial prefrontal cortex is critical to the development of sensitization to amphetamine. There is evidence that psychostimulant-induced changes in dopamine-GABA interactions are key to understanding the behaviorally sensitized response. The objective of this study was to cha...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.07.047
更新日期:2007-11-09 00:00:00
abstract::In vivo microdialysis was used to measure the effects of partial deafferentation of cortical cholinergic inputs on acetylcholine efflux in young (four to seven months) and aged (24-28 months) male F344/BNNIA rats. Partial deafferentation was produced by bilateral infusions of the immunotoxin 192 immunoglobulin G-sapor...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00515-6
更新日期:1999-03-01 00:00:00
abstract::The neurotensin-containing projections from the retrosplenial cortex to the anterior ventral thalamus were demonstrated by electrolytic lesion studies and fluorescent retrograde tracing combined with immunocytochemistry. Three-to-five-day-old rats were used, because the immunoreactivity of neurotensin fibers in anteri...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(88)90102-9
更新日期:1988-09-01 00:00:00
abstract::Estrogens influence neuronal activity and are important for normal brain functions. Effects of estrogens on seizures are contradictory. It is commonly accepted that estrogens may increase neuronal excitability and thus mediate proconvulsant effects. However, clinical and animal data show that estrogen may also have no...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2005.07.005
更新日期:2006-01-01 00:00:00
abstract::Having provided an anatomical description of the efferent projections of the dorsal midbrain anticonvulsant zone [Shehab S. et al. (1995) Neuroscience 65, 681-695], our purpose in the present study was to establish which outputs from this region are responsible for mediating the anticonvulsant and behavioural properti...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)00517-9
更新日期:1995-04-01 00:00:00
abstract::Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease that affects upper and lower motor neurons and leads to death a few years after symptom onset. Despite its high morbidity and mortality, its underlying pathogenic mechanisms still remain poorly understood. Although there is increasing evidence for sign...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.07.047
更新日期:2020-12-01 00:00:00
abstract::Riluzole, an anti-amyotrophic lateral sclerosis drug, known to decrease presynaptic glutamate release, is viewed as a candidate supplementary medication for epilepsy. In the present study, we compared the effects of riluzole and valproate (VPA) in the pilocarpine-induced limbic seizure model and in the gamma-hydroxybu...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.04.018
更新日期:2007-06-15 00:00:00
abstract::An odorant's code is represented by activity in a dispersed ensemble of olfactory sensory neurons in the nose, activation of a specific combination of groups of mitral cells in the olfactory bulb and is considered to be mapped at divergent locations in the olfactory cortex. We present here an in vitro model of the mam...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.07.071
更新日期:2008-10-28 00:00:00
abstract::The primary somatosensory cortex (S1) projects to the thalamus and brainstem somatosensory nuclei and modulates somatosensory information ascending to the S1 itself. However, the projections from the S1 to the brainstem second-order somatosensory neuron pools have not been fully studied. To address this in rats, we fi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.10.048
更新日期:2012-01-03 00:00:00
abstract::It has been proposed that two amino acid substitutions in the transcription factor FOXP2 have been positively selected during human evolution and influence aspects of speech and language. Recently it was shown that when these substitutions are introduced into the endogenous Foxp2 gene of mice, they increase dendrite l...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.11.042
更新日期:2011-02-23 00:00:00
abstract::Paclitaxel is one of the most commonly used anti-neoplastic drugs for the treatment of solid tumors. Unfortunately, its use is often associated with dose-limiting painful peripheral neuropathy and subsequent neuropathic pain that is resistant to standard analgesics. However, there are few clinically available drugs or...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.11.046
更新日期:2011-02-03 00:00:00
abstract::Efferent projections of the zona incerta were examined in the rat using the autoradiographic and horseradish peroxidase methods, with special reference to the cytoarchitectonic structure of the zona incerta. Autoradiographic experiments showed that the incertofugal fiber systems reach ipsilaterally to the thalamus (la...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(82)90203-2
更新日期:1982-10-01 00:00:00
abstract::Oxytocin regulates partner preference formation and alloparental behavior in the socially monogamous prairie vole (Microtus ochrogaster) by activating oxytocin receptors in the nucleus accumbens of females. Mating facilitates partner preference formation, and oxytocin-immunoreactive fibers in the nucleus accumbens hav...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.05.055
更新日期:2009-09-15 00:00:00