Abstract:
:Advanced glycation end-products comprise a complex and heterogeneous group of compounds that have been implicated in diabetes-related complications. The importance of the Maillard reaction is depicted by the formation of reactive intermediate products known as α-oxoaldehydes, such as 3-deoxyglucosone (3-DG). This product has been found to be involved in accelerated vascular damage in diabetes. In the present study, calf thymus histone H2A was reacted with 3-DG, and the generation of advanced glycation end products was investigated by determining the degree of side chain modifications (lysine and arginine residues), Amadori products, carbonyl content, N(ε) -carboxymethyl lysine, and pentosidine using various physicochemical techniques. Moreover, fluorescence, absorbance as well as structural characteristics of glycated-H2A were comprehensively investigated. Overall, this study demonstrates structural perturbation, formation of different intermediates, and AGEs that are believed to hamper the normal functioning of H2A histone, compromising the integrity of chromatin structures and function in secondary complications of diabetes.
journal_name
IUBMB Lifejournal_title
IUBMB lifeauthors
Ashraf JM,Ahmad S,Rabbani G,Jan AT,Lee EJ,Khan RH,Choi Idoi
10.1002/iub.1318subject
Has Abstractpub_date
2014-10-01 00:00:00pages
686-93issue
10eissn
1521-6543issn
1521-6551journal_volume
66pub_type
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