Abstract：
:The reaction between nitric oxide ((.)NO) and oxygen yields reactive species capable of oxidizing and nitrosating proteins, as well as deaminating DNA bases. Although this reaction is considered too slow to be biologically relevant, it has been shown that membranes, lipoproteins, mitochondria and possibly proteins can accelerate this reaction. This effect stems from the higher solubility of both (.)NO and O(2)in the hydrophobic phase of these biological particles, leading to a concentration of both reagents and so a higher rate of reaction. It has been determined that this reaction occurs from 30 to 300 times more rapidly within the membrane, while even higher values have been suggested for proteins. The autoxidation of (.)NO in membranes is not the main route for cellular (.)NO consumption but an important consequence of this phenomenon is to focus the generation of significant amounts of oxidizing and nitrosating molecules (nitrogen dioxide and dinitrogen trioxide) in the small volume comprised by cellular membranes. Even so, these reactive species are diffusible and their ultimate fate will depend on the reactivity towards available substrates rather than on physical barriers. The acceleration of (.)NO autoxidation by biological hydrophobic phases may thus be a general phenomenon that increases in importance in cases of (.)NO overproduction.
journal_name
IUBMB Lifejournal_title
IUBMB lifeauthors
Möller MN,Li Q,Lancaster JR Jr,Denicola Adoi
10.1080/15216540701311147subject
Has Abstractpub_date
2007-04-01 00:00:00pages
243-8issue
4-5eissn
1521-6543issn
1521-6551pii
778719214journal_volume
59pub_type
杂志文章,评审相关文献
IUBMB LIFE文献大全abstract：:The expression of the cytM gene, which encodes cytochrome cM in Synechocystis sp. PCC 6803, is induced under stress conditions such as low temperature. Results of spectrophotometric studies revealed that cytochrome cM was oxidized by light absorbed by photosystem I in cells that had acclimatized to a low temperature. ...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1080/15216540118716
更新日期：2001-02-01 00:00:00
abstract：:The purpose of this study was to confirm protein-protein interaction between cyclooxygenase-1 (COX-1) and caveolins. The interaction of cyclooxygenase-1 and caveolins in the cultured human embryonic kidney (HEK 293) cells was investigated using immuno-precipitation and Western blot analysis. In HEK 293 cells, high lev...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1080/15216540410001699312
更新日期：2004-04-01 00:00:00
abstract：:Cartilage engineering is one challenging issue in regenerative medicine. Low oxygen tension or hypoxia inducible factor-1 (HIF-1α) gene therapy are promising strategies in the field of cartilage repair. Previously, we showed that hypoxia and its mediator HIF-1 regulate matrix genes expression (collagens and aggrecan)....
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1002/iub.1539
更新日期：2016-09-01 00:00:00
abstract：:Ovarian cancer (OC) is one of the most lethal gynaecological cancers, which usually has a poor prognosis due to late diagnosis. A large percentage of the OC cell population is in a nonproliferating and quiescent stage, which poses a barrier to success when using most chemotherapeutic agents. Recent studies have shown ...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1002/iub.1035
更新日期：2012-07-01 00:00:00
abstract：:Malaria remains a worldwide health concern with an estimated quarter of a billion people infected and nearly half a million deaths annually. Malaria is caused by a parasite infection from Plasmodium strains which are transmitted from mosquitoes into the human host. Although several small molecule inhibitors have been ...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1002/iub.2244
更新日期：2020-06-01 00:00:00
abstract：:Transforming growth factorbeta1 (TGFbeta1) is considered to be the principal contributor to liver fibrosis. So in this study the ribozymes against TGFbeta1 were designed. The in vitro cleavage activities of the ribozymes were assayed through incubation of (32)p-labeled target RNAs and (32)p-labeled ribozymes in differ...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1080/15216540400024470
更新日期：2005-01-01 00:00:00
abstract：:In the present study we demonstrated Jurkat leukemia cell lines of TIB152 and TIB153 with different sensitivities to recombinant soluble TRAIL cytotoxicity. TRAIL receptor death receptor 5 (DR5) was constitutively localized in the rafts in both cell lines. FADD, caspase-8, and PI3K-p85 subunit were recruited into DR5 ...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1002/iub.166
更新日期：2009-03-01 00:00:00
abstract：:In this article, we discuss single molecule observation of rotational catalysis by E. coli ATP synthase (F-ATPase) using small gold beads. Studies involving a low viscous drag probe showed the stochastic properties of the enzyme in alternating catalytically active and inhibited states. The importance of subunit intera...
journal_title：IUBMB life
pub_type： 杂志文章,评审
doi：10.1002/iub.1134
更新日期：2013-03-01 00:00:00
abstract：:Cell-surface-bound extracellular DNA (csbDNA) is present on the outer membrane of blood cells, including both red blood cells and leukocytes. Although less well characterized than cell-free DNA (cfDNA) in plasma and serum, leukocyte and red blood cell csbDNA form a considerable fraction of the blood extracellular nucl...
journal_title：IUBMB life
pub_type： 杂志文章,评审
doi：10.1002/iub.2070
更新日期：2019-09-01 00:00:00
abstract：:We hypothesize that the various, previously reported, reactivities of neuroglobin with redox partners and oxygen provide for the establishment of a redox cycle within cells, such as neurons and retinal rod cells. Using native cell lysates, from cultured human cells of neuronal origin, we have estimated the rate of red...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1002/iub.566
更新日期：2012-05-01 00:00:00
abstract：:Lysosomal storage disorders (LSDs) are inherited metabolic diseases caused by deficiencies in lysosomal proteins, which result in accumulation of undegraded metabolites and disruption of lysosomal proteostasis. Despite significant progress in the molecular genetics and biochemistry underlying the cellular pathogenesis...
journal_title：IUBMB life
pub_type： 杂志文章,评审
doi：10.1002/iub.1288
更新日期：2014-07-01 00:00:00
abstract：:Members of the GATA family of transcription factors play key roles in the differentiation of specific cell lineages by regulating the expression of target genes. Three GATA factors play distinct roles in hematopoietic differentiation. In order to better understand how these GATA factors function to regulate genes thro...
journal_title：IUBMB life
pub_type： 杂志文章,评审
doi：10.1002/iub.2195
更新日期：2020-01-01 00:00:00
abstract：:Red blood cells of yellow-spotted river turtles (Podocnemis unifilis, Pleurodira, Chelonia, REPTILIA) have two hemoglobin (Hb) components, Hb A and Hb D. We purified the hemoglobin component homologous to amniote (reptiles, birds, and mammals) adult Hb A which comprises two identical α(A) -globin polypeptides and two ...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1002/iub.429
更新日期：2011-03-01 00:00:00
abstract：:The beta/alpha-barrel motif was once considered to be a single protein domain. In recent years, however, it has been shown to consist of smaller substructures displaying the ability to fold autonomously. Here we review the current status of experimental findings concerning the motif's folding behavior in the light of ...
journal_title：IUBMB life
pub_type： 杂志文章,评审
doi：10.1080/15216540214924
更新日期：2002-10-01 00:00:00
abstract：:Endothelial dysfunction is fundamental to ischemic stroke and brain injury. The humanin analogue S14G-humanin (HNG) has been shown to be a cytoprotective derivative. In this study, we investigated the neuroprotective effects of HNG in vivo and in vitro. In a murine middle cerebral artery occlusion (MCAO) stroke model,...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1002/iub.1869
更新日期：2018-07-01 00:00:00
abstract：:The aggressive biological behavior of invasive and metastatic cancer is considered to be the most insidious and life-threatening aspect for breast cancer patients. It is mostly the result of changes in many molecular characteristics of tumor cells, including alterations in gene expression and the balance of proteolyti...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1080/15216540701395074
更新日期：2007-06-01 00:00:00
abstract：:In many organisms the processes of DNA replication and recombination are closely linked. For instance, in bacterial and eukaryotic systems, replication forks can become stalled or damaged, in many cases leading to the formation of double stranded breaks. Replication restart is an essential mechanism in which the recom...
journal_title：IUBMB life
pub_type： 杂志文章,评审
doi：10.1080/15216540310001612237
更新日期：2003-08-01 00:00:00
abstract：:In this study, we intended to determine the detailed function and mechanism of long noncoding RNA (lncRNA) colorectal neoplasia differentially expressed (CRNDE) in liver injury induced by sepsis. Cecal ligation and perforation (CLP) models were adopted to induce sepsis in vivo with rats, and hepatic epithelial cells L...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1002/iub.2230
更新日期：2020-03-01 00:00:00
abstract：:Methionine (Met) is a neutral, sulfur-containing, essential amino acid with biological functions in the initiation and prolongation step of protein synthesis, transmethylation reactions, the synthesis of cysteine and cystine, and as a component of antioxidant systems. Its key importance is reflected by the fact that i...
journal_title：IUBMB life
pub_type： 杂志文章,评审
doi：10.1002/iub.1571
更新日期：2016-12-01 00:00:00
abstract：:Availability of the human genome data has enabled the exploration of a huge amount of biological information encoded in it. There are extensive ongoing experimental efforts to understand the biological functions of the gene products encoded in the human genome. However, computational analysis can aid immensely in the ...
journal_title：IUBMB life
pub_type： 杂志文章,评审
doi：10.1080/15216540400006105
更新日期：2004-06-01 00:00:00
abstract：:Myosin light chain kinase (MLCK) is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20-kDa myosin light chain (MLC20) to activate the myosin ATPase activity. Although this mode of action is well-established, there are numerous reports of smooth muscle contraction that is not associate...
journal_title：IUBMB life
pub_type： 杂志文章,评审
doi：10.1080/152165401753366087
更新日期：2001-06-01 00:00:00
abstract：:The formation, maturation, and dissolution of focal adhesions are basic prerequisites of cell migration and rely on the recruitment, signalling, and endocytosis of integrins. In many instances, extracellular matrix molecules are recognised by a number of integrins, and it is the sequential involvement of different int...
journal_title：IUBMB life
pub_type： 杂志文章,评审
doi：10.1002/iub.200
更新日期：2009-07-01 00:00:00
abstract：:In protein and RNA macromolecules, only a limited number of different side-chain chemical groups are available to function as catalysts. The myriad of enzyme-catalyzed reactions results from the ability of most of these groups to function either as nucleophilic, electrophilic, or general acid-base catalysts, and the k...
journal_title：IUBMB life
pub_type： 杂志文章,评审
doi：10.1080/15216540211468
更新日期：2002-02-01 00:00:00
abstract：:Besides its classical mode of action through activation of specific receptors at the cell surface, fibroblast growth factor 1 (FGF1) can also cross the cellular membrane and translocate into the cytosol and further to the nucleus. The mechanism of this translocation is described partially, but the role of FGF1 inside ...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1002/iub.1480
更新日期：2016-03-01 00:00:00
abstract：:Myocardial ischemia/reperfusion (I/R) injury greatly contributes to myocardial tissue damage in patients with coronary disease, which eventually leads to heart failure. Long noncoding RNAs (lncRNAs) have an emerging role in the process of myocardial I/R injury. Our previous work revealed the protective role of miR-374...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1002/iub.2428
更新日期：2020-12-09 00:00:00
abstract：:Prostate cancer (PCa) is considered the most common malignancy in men. The aim of this study is to assess the role of serum miR-15a and miR-16-1 expression in PCa development, diagnosis and prognosis aiming to find a specific noninvasive biomarker. This study comprised 70 patients with PCa, 70 patients complaining of ...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1002/iub.1733
更新日期：2018-05-01 00:00:00
abstract：:Implantation is a complex developmental event that is initiated by recognition and adhesion of the embryo to the endometrial epithelium. sLeX is an oligosaccharide antigen acting as the ligand of L-selectin, and is stage-specifically expressed in the endometrial epithelium. The adhesion system mediated by L-selectin a...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1002/iub.62
更新日期：2008-07-01 00:00:00
abstract：:Recent successes in identifying the genes and associated proteins underlying several familial neurodegenerative conditions have not always resulted in accounts as to why the associated patterns of neuronal damage are so specific and limited. Here, with reference to Huntington's disease, we present a general scheme to ...
journal_title：IUBMB life
pub_type： 杂志文章,评审
doi：10.1080/1521654031000153012
更新日期：2003-06-01 00:00:00
abstract：:Although the amino acid sequences and the structures of pyruvate kinase (PYK) isozymes are highly conserved, allosteric regulations differ. This suggests that amino acids with low conservation play important roles in the allosteric mechanism. The current work exploits a 'natural screen'- the 122 point mutations identi...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1080/15216540500531705
更新日期：2006-01-01 00:00:00
abstract：:Cardiac hypoxia plays a significant role in various types of heart disease, and improper treatment of hypoxia often leads to myocardial cell damage or even death. Transcriptome profiling and open chromatin mapping have been used as powerful tools to understand the development of heart disease, but the interplay betwee...
journal_title：IUBMB life
pub_type： 杂志文章
doi：10.1002/iub.2297
更新日期：2020-08-01 00:00:00
