Abstract:
:Sorafenib is the approved systemic drug of choice for advanced hepatocellular carcinoma (HCC), but has demonstrated limited benefits because of drug resistance. 2-Methoxyestradiol (2ME2) has been shown to be a promising anticancer drug against various types of cancers and acts by dysregulating hypoxia-inducible factor (HIF)-1. Hypoxic cancer cells are extremely resistant to therapies since they elicit strong survival ability due to the cellular adaptive response to hypoxia, which is controlled by HIF-1 and HIF-2. The present study has demonstrated that sorafenib downregulated the expression of HIF-1α, making the hypoxic response switch from HIF-1α- to HIF-2α-dependent pathways, resulting in upregulation of HIF-2α, which contributes to the insensitivity of hypoxic HCC cells to sorafenib. HIF-2α played a dominant role in regulating VEGF, thus sorafenib in turn increased the expression of VEGF (a downstream molecule of both HIF-1 and HIF-2) and cyclin D1 (a downstream molecule of HIF-2), but reduced the expression of LDHA (a downstream molecule of HIF-1), in hypoxic HCC cells. 2ME2 significantly reduced the expression of both HIF-1α and HIF-2α, and their downstream molecules, VEGF, LDHA and cyclin D1, rendering hypoxic HCC cells to increased sensitivity to 2ME2. 2ME2 also inhibited the nuclear translocation of HIF-1α and HIF-2α proteins, but had no effect on their mRNA expression. 2M2 synergized with sorafenib to suppress the proliferation and induction of apoptosis of HCC cells in vitro and in vivo, and inhibited tumoral angiogenesis. These results indicate that 2ME2 given in combination with sorafenib acts synergistically for treating HCC.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Ma L,Li G,Zhu H,Dong X,Zhao D,Jiang X,Li J,Qiao H,Ni S,Sun Xdoi
10.1016/j.canlet.2014.09.011subject
Has Abstractpub_date
2014-12-01 00:00:00pages
96-105issue
1eissn
0304-3835issn
1872-7980pii
S0304-3835(14)00510-2journal_volume
355pub_type
杂志文章相关文献
CANCER LETTERS文献大全abstract::Neuroblastoma (NB) is the most common extracranial solid tumor in early childhood. Despite intensive multimodal therapy, nearly half of children with high-risk disease will relapse with therapy-resistant tumors. Dysregulation of MAPK pathway has been implicated in the pathogenesis of relapsed and refractory NB patient...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2019.05.011
更新日期:2019-08-10 00:00:00
abstract::The effect of vitamin A (retinyl acetate) on lung tumor development in strain A mice exposed to radiation was assessed. Four groups of 75 mice were utilized. Two groups were fed a low vitamin A diet (less than 100 IU/100 g diet) and the other 2 were fed a high vitamin A diet (800 IU/100 g diet). After 2 weeks one grou...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(84)90051-x
更新日期:1984-02-01 00:00:00
abstract::Not-targeted effects represent a paradigm shift from the "DNA centric" view that ionizing radiation only elicits biological effects and subsequent health consequences as a result of an energy deposition event in the cell nucleus. While this is likely true at higher radiation doses (>1 Gy), at low doses (<100 mGy) non-...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2013.09.009
更新日期:2015-01-01 00:00:00
abstract::To identify novel anti-cancer agents, we created and screened a unique nutraceutical library for activity against acute myeloid leukemia (AML) cells. From this screen, we determined that glucopsychosine was selectively toxic toward AML cell lines and primary AML patient samples with no effect toward normal hematopoiet...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2014.03.003
更新日期:2014-06-28 00:00:00
abstract::The immunomodulatory and antimetastatic/antitumor activity of thymosin alpha(1) (Talpha(1)) was evaluated in BALB/c-mice. Daily subcutaneous application (7 consecutive days, 0.01-10 microg of Talpha(1)/injection per mouse) upregulated the number of thymocytes and peripheral blood cells in tumor bearing mice. To check ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(00)00510-3
更新日期:2000-10-16 00:00:00
abstract::MUTYH-associated polyposis (MAP) is an autosomal recessive condition predisposing to colorectal cancer, caused by constitutional biallelic mutations in the base excision repair (BER) gene MUTYH. Colorectal tumours from MAP patients display an excess of somatic G>T mutations in the APC and KRAS genes due to defective B...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2008.09.022
更新日期:2009-02-18 00:00:00
abstract::Agents antagonizing the anti-apoptotic Bcl-2 protein have been shown to restore normal apoptotic processes in cancer cells. Since upregulation of Bcl-2 is often observed in recurrent or refractory hematological malignancies, we were prompted to investigate whether drug-selected leukemia cells overexpressing Bcl-2 were...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2007.01.018
更新日期:2007-08-08 00:00:00
abstract::The primary aim of this study was to find novel chemopreventive agents effective against breast cancer. Endoplasmic reticulum (ER) stress can induce apoptosis through the unfolded protein response (UPR). 2'-Hydroxy-2,3,5'-trimethoxychalcone (DK143) is a synthetic flavonoid derivative. The present study provides eviden...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2015.12.017
更新日期:2016-03-01 00:00:00
abstract::First-generation EGFR tyrosine kinase inhibitors (TKIs) such as erlotinib have significant activity in NSCLC patients with activating EGFR mutations. However, EGFR-TKI resistance inevitably occurs after approximately 12 months of treatment. Acquired mechanisms of resistance, other than secondary mutations in EGFR (T79...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2020.05.009
更新日期:2020-08-28 00:00:00
abstract::The purpose of this study was to investigate the possible role of PI-3K/Akt2 pathway in docetaxel-induced apoptosis. Here we showed that transfection of full-length Akt2 into breast and ovarian cancer cells could provoke Akt phosphorylation and induce an enhanced resistance to docetaxel. FN adhesion promoted Akt phosp...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2007.11.022
更新日期:2008-03-08 00:00:00
abstract::DDX1, a gene mapping to the 2p24 region, has been observed to be co-amplified with MYCN in neuroblastoma. Co-amplification of the DDX1 gene is a consequence of the short physical distance between the two genes. Recently, it has been found that neuroblastoma cells can show a low increase in MYCN gene copy number, defin...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2007.05.010
更新日期:2007-10-18 00:00:00
abstract::To address the possible involvement of hyperinsulinemia in breast cancer development, we have examined the susceptibility of lean and obese Zucker rats to N-methyl-N-nitrosourea (MNU)-induced mammary cancer. Fifty-day-old female lean or obese Zucker rats received intraperitoneal (i.p.) injections of 37.5 or 20 mg/kg M...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(00)00635-2
更新日期:2001-01-26 00:00:00
abstract::We previously demonstrated that evening primrose extract (EPE) induced apoptosis in Ehrlich ascites tumor cells (EATC), and this effect was specific on tumor cells. Furthermore, our results demonstrated that EPE exposure elicited a rapid increase in the activity of superoxide dismutase and intracellular peroxides leve...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2003.11.001
更新日期:2004-04-15 00:00:00
abstract::The incidence and pattern of ras oncogene mutations in human malignancies demonstrate geographic and racial differences. For example, specificity of alterations is found in cholangiocellular carcinomas in Thai patients with a different etiology from those in Japanese patients. In the present study, a comparison of ras...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(98)00149-9
更新日期:1998-09-25 00:00:00
abstract::Although the incidence is very low, the prognosis of anaplastic carcinoma of the thyroid is very poor regardless of the results of various therapeutic trials. We found that the mechanism of anti-cancer drug resistance in anaplastic carcinoma of the thyroid was not explicable only in terms of expression of the mdr1 and...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(94)90010-8
更新日期:1994-07-29 00:00:00
abstract::Ring1 and YY1 binding protein (RYBP) is a member of the Polycomb group (PcG) proteins and regulates cell growth through both PcG-dependent and -independent mechanisms. Our initial study indicated that RYBP is down-regulated in human non-small cell lung cancer (NSCLC) tissues. The present study determined the molecular...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2015.09.003
更新日期:2015-12-28 00:00:00
abstract::Carcinogenic and modification potential of 6-mercaptopurine (6-MP) was studied in a medium-term bioassay system for rat liver carcinogenesis. F344 male rats were initiated with a single dose (200 mg/kg body wt.) of diethylnitrosamine (DEN) i.p. and fed diets containing either 0.005% or 0.02% 6-MP with or without 0.05%...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(92)90069-8
更新日期:1992-06-15 00:00:00
abstract::Monoclonal antibodies were produced and characterized in order to allow the monitoring of the urinary excretion of six modified nucleosides. The specificity of each antibody was determined and competitive solid-phase enzyme-linked immunoassays were designed, the sensitivity of which lay in the pmol range. Detection an...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(92)90296-8
更新日期:1992-01-31 00:00:00
abstract::Sulfasalazine (SSZ) is an anti-inflammatory drug that has been demonstrated to induce apoptosis and tumor regression through inhibition of plasma membrane cystine transporter xc(-). Cysteine is a rate-limiting precursor for intracellular glutathione (GSH) synthesis, which is vital for compound detoxification and maint...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2015.07.031
更新日期:2015-11-01 00:00:00
abstract::Chronic exposure to cadmium is known to be a risk factor for human prostate cancer. Despite over-whelming evidence of cadmium causing carcinogenicity in humans, the specific underlying molecular mechanisms that govern metal-induced cellular transformation remain unclear. Acute exposure (up to 72 h) to cadmium induces ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2017.08.023
更新日期:2017-11-01 00:00:00
abstract::Aberrant activation of signal transducer and activator of transcription 3 (STAT3) is frequently observed in multiple myeloma (MM) cancer and can upregulate the expression of several genes involved in proliferation, survival, metastasis, and angiogenesis. The effect of farnesol (FOH) on STAT3 activation, associated pro...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2015.02.024
更新日期:2015-05-01 00:00:00
abstract::N-[2-(p-bromocinnamylmethylamino) ethyl]-5-isoquinolinesulfonamide (H-87), a newly synthesized inhibitor of protein kinase A, significantly decreased the drug resistance of multidrug resistant human U937/M cells, mouse FM3A/M and P388/M cells. Northern blot analysis showed MDR1 gene expression was decreased in H-87-tr...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(93)90041-7
更新日期:1993-10-15 00:00:00
abstract::Dioxane, perchloroethylene, and trichloroethylene were investigated as rat liver altered foci promoters in an initiation/promotion protocol. Animals were initiated with diethylnitrosamine, 30 mg/kg, injected i.p. 24 h after 2/3 partial hepatectomy. The chemical under study was administered by gavage once a day, 5 time...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(87)90099-1
更新日期:1987-07-01 00:00:00
abstract::We have evaluated the frequency of HLA class I and II antigens in 205 Italian patients with hepatocellular carcinoma (HCC) and 749 blood donors (controls). Moreover, we have looked for correlations between HLA antigen frequencies and HBV and/or HCV infections in HCC patients. We found great differences in HLA antigen ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:
更新日期:1995-11-27 00:00:00
abstract::alpha-Toxin (alpha-hemolysin) of Staphylococcus aureus is a pore-forming bacterial toxin which after caveolin-1-dependent assembly induces apoptosis in eukaryotic cells. We investigated if a sub-toxic concentration of staphylococcal alpha-toxin could enhance cisplatin-induced apoptosis and overcome acquired cisplatin-...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2008.02.007
更新日期:2008-06-28 00:00:00
abstract::Modifying effects of 5-hydroxy-4-(2-phenyl-(E)-ethenyl)-2(5H)-furanone, a novel synthesized retinoid (KYN-54), on intestinal carcinogenesis were examined in a rat model using azoxymethane (AOM). A total of ninety male F344 rats, 6 weeks old, were divided into 4 groups. Group 1 (20 rats) was fed a diet containing KYN-5...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(92)90220-p
更新日期:1992-09-30 00:00:00
abstract::Fluorescence-activated cell sorting (FACS) based on the surface marker CD133 is the most common method for isolating glioma stem cells (GSCs) from heterogeneous glioma cell populations. Optimization of this method will have profound implications for the future of GSC research. Five commonly used digestion reagents, Li...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2016.04.022
更新日期:2016-07-10 00:00:00
abstract::Urethane-induced lung tumors and their genetic changes were investigated in transgenic (Tg) mice carrying a human prototype c-Ha-ras gene (rasH2 mice). Male and female rasH2 mice and non-transgenic (non-Tg) littermates were injected intraperitoneally with 1000 mg/kg of urethane once or three times at 2-day intervals. ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(00)00372-4
更新日期:2000-05-29 00:00:00
abstract::Considerable evidences have shown that both heat shock transcription factor 1 (HSF1) and autophagy can attenuate the sensitivity of hepatocellular carcinoma (HCC) cells to chemotherapeutic reagents. However, it is still little known whether HSF1 is associated with autophagy in regulating the chemosensitivity of HCC ce...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2017.08.039
更新日期:2017-11-28 00:00:00
abstract::Highly active antiretroviral therapy (HAART) changed the natural history of pediatric HIV infection. This review focuses on trends of HIV-associated cancers in childhood in the HAART era and analyses potential pathogenetic mechanisms. HAART reduced AIDS-defined-malignancies (ADM), but incidence of several non-ADM is i...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2014.02.002
更新日期:2014-05-28 00:00:00