当前位置： SCI文献检索 > CANCER LETTERS期刊下所有文献 > Optimized dissociation protocol for isolating human glioma stem cells from tumorspheres via fluorescence-activated cell sorting.

Optimized dissociation protocol for isolating human glioma stem cells from tumorspheres via fluorescence-activated cell sorting.

Abstract：

:Fluorescence-activated cell sorting (FACS) based on the surface marker CD133 is the most common method for isolating glioma stem cells (GSCs) from heterogeneous glioma cell populations. Optimization of this method will have profound implications for the future of GSC research. Five commonly used digestion reagents, Liberase-TL, trypsin, TrypLE, Accutase, and non-enzymatic cell dissociation solution (NECDS), were used to dissociate glioma tumorspheres derived from two primary glioma specimens (091214 and 090116) and the cell lines U87 and T98G. The dissociation time, cell viability, retention of CD133, and stemness capacity were assessed. The results showed that single cells derived from the Liberase-TL (200 µg/ml) group exhibited high viability and less damage to the antigen CD133. However, the efficiency of NECDS for dissociating the tumorspheres into single cells was fairly low. Meanwhile, the use of this digestion reagent resulted in obvious cellular and antigenic impairments. Taken together, Liberase-TL (200 µg/ml) is an ideal reagent for isolating GSCs from tumorspheres. In contrast, the use of NECDS for such a protocol should be carefully considered.

journal_name

Cancer Lett

journal_title

Cancer letters

authors

Lv D,Ma QH,Duan JJ,Wu HB,Zhao XL,Yu SC,Bian XW

doi

10.1016/j.canlet.2016.04.022

subject

Has Abstract

pub_date

2016-07-10 00:00:00

pages

105-15

issue

1

eissn

0304-3835

issn

1872-7980

pii

S0304-3835(16)30257-9

journal_volume

377

pub_type

杂志文章

相关文献

CANCER LETTERS文献大全
  • Effect of methylazoxymethanol acetate on adenylate cyclase and 5'-nucleotidase in rat liver plasma membranes.

    abstract：:A single tumorigenic dose of methylazoxymethanol acetate increased adenylate cyclase activity in total liver homogenates 70-100% by the seventh day after treatment. The increased activity occurred in the plasma membranes rather than in the nuclei and was accompanied by a significant increase in 5'-nucleotidase activit...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/s0304-3835(75)95764-x

    authors： Zedeck MS,Swislocki NI

    更新日期：1975-11-01 00:00:00

  • Inhibition of MDR1 gene expression by H-87, a selective inhibitor of cAMP-dependent protein kinase.

    abstract：:N-[2-(p-bromocinnamylmethylamino) ethyl]-5-isoquinolinesulfonamide (H-87), a newly synthesized inhibitor of protein kinase A, significantly decreased the drug resistance of multidrug resistant human U937/M cells, mouse FM3A/M and P388/M cells. Northern blot analysis showed MDR1 gene expression was decreased in H-87-tr...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/0304-3835(93)90041-7

    authors： Kim SH,Park JI,Chung BS,Kang CD,Hidaka H

    更新日期：1993-10-15 00:00:00

  • N-nitrosation and N-nitration of morpholine by nitrogen dioxide: inhibition by ascorbate, glutathione and alpha-tocopherol.

    abstract：:Ascorbate anion and glutathione were found to inhibit the aqueous reaction between nitrogen dioxide (NO2) and morpholine (MOR) and thereby prevented the formation of N-nitrosomorpholine (NMOR) and N-nitromorpholine (NTMOR) at both pH 7.4 and 12.5. These antioxidants are approximately 3 orders of magnitude more reactiv...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/0304-3835(86)90042-x

    authors： Cooney RV,Ross PD,Bartolini GL

    更新日期：1986-07-01 00:00:00

  • Aurora-A amplification associated with BRCA2 mutation in breast tumours.

    abstract：:Potential interaction of Aurora-A amplification and BRCA2 mutation was examined in breast tumours from BRCA2 999del5 mutation carriers (n=20) and non-carriers (n=41). Aurora-A amplification studied by FISH was significantly more common in breast tumours from BRCA2 mutation carriers (p=0.0005). Extensive Aurora-A ampli...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/j.canlet.2006.06.003

    authors： Bodvarsdottir SK,Hilmarsdottir H,Birgisdottir V,Steinarsdottir M,Jonasson JG,Eyfjord JE

    更新日期：2007-04-08 00:00:00

  • Cancer mortality and some socio economic correlates in Rosario, Argentina.

    abstract：:The age-standardized mortality rates by sex for cancer of all sites and some specific sites were calculated in the 22 areas of Rosario (Argentina). The rates by area were correlated with education, occupation, housing quality and population density, applying Kendall's tau. Cervical cancer rates showed the strongest co...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/0304-3835(90)90159-u

    authors： Poletto L,Morini JC

    更新日期：1990-03-01 00:00:00

  • Translational control: a target for cancer therapy.

    abstract：:Cap-mediated translation is the default mechanism for the synthesis of proteins in eukaryotic cells. Increasingly, malignant cells have been found to have deregulation of this process. Return of normal translational control is associated with loss of tumorigenic potential in pre-clinical models. Currently, a variety o...

    journal_title：Cancer letters

    pub_type： 杂志文章,评审

    doi：10.1016/j.canlet.2007.08.022

    authors： Thumma SC,Kratzke RA

    更新日期：2007-12-08 00:00:00

  • Effect of glucocorticoids on TPA-induced inhibition of gap-junctional communication and morphological transformation in Syrian hamster embryo cells.

    abstract：:The effect of glucocorticoids on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inhibition of gap-junctional intercellular communication (GJIC) and morphological transformation in Syrian hamster embryo (SHE) cells was examined. Fluocinolone acetonide (FA) and dexamethasone (DEX) almost completely suppressed the ef...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/0304-3835(93)90006-u

    authors： Roseng LE,Rivedal E

    更新日期：1993-08-16 00:00:00

  • The cytotoxicity of Pseudomonas exotoxin A, inactivated by modification of the cell-binding domain I, is restored when conjugated to an erythroid cell-specific targeting agent.

    abstract：:To be capable of selective killing of tumor cells, the non-selective Pseudomonas aeruginosa exotoxin A must have its cell-binding domain inactivated or removed and then be chemically linked to, or genetically fused with, a specific targeting agent. In the present study, epsilon-NH2 groups of lysine residues of the cel...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/0304-3835(90)90241-o

    authors： Bourdenet S,Doyonnas R,Vacheron MJ,Guinand M,Fasciotto B,Ristic A,Michel G,Cozzone AJ,Durkin JP,Whitfield JF

    更新日期：1990-04-20 00:00:00

  • Tea and health: a historical perspective.

    abstract：:In many parts of the world, green tea and black tea are produced from the plant Camellia sinensis. Tea is one of the most widely consumed beverages, second only to water. It is one of the safest beverages since it is made with boiling, sterile water and has been popular for over 4000 years. Dogma has it that people kn...

    journal_title：Cancer letters

    pub_type： 历史文章,杂志文章

    doi：10.1016/s0304-3835(97)04691-0

    authors： Weisburger JH

    更新日期：1997-03-19 00:00:00

  • DLK1, delta-like 1 homolog (Drosophila), regulates tumor cell differentiation in vivo.

    abstract：:The stem cell-like characteristics of tumor cells are not only essential for tumor development and malignant progression, but also significantly contribute to therapy resistance. However, it remains poorly understood how cancer cell differentiation or stemness is regulated in vivo. We investigated the role of the stem...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/j.canlet.2011.11.032

    authors： Begum A,Kim Y,Lin Q,Yun Z

    更新日期：2012-05-01 00:00:00

  • Nintedanib, a triple angiokinase inhibitor, enhances cytotoxic therapy response in pancreatic cancer.

    abstract：:Angiogenesis remains a sensible target for pancreatic ductal adenocarcinoma (PDAC) therapy. VEGF, PDGF, FGF and their receptors are expressed at high levels and correlate with poor prognosis in human PDAC. Nintedanib is a triple angiokinase inhibitor that targets VEGFR1/2/3, FGFR1/2/3 and PDGFRα/β signaling. We invest...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/j.canlet.2014.12.027

    authors： Awasthi N,Hinz S,Brekken RA,Schwarz MA,Schwarz RE

    更新日期：2015-03-01 00:00:00

  • EGCG induces apoptosis in human laryngeal epidermoid carcinoma Hep2 cells via mitochondria with the release of apoptosis-inducing factor and endonuclease G.

    abstract：:(-)-Epigallocatechin-3-gallate (EGCG), a major green tea polyphenol, was tested for in vitro cytotoxicity against human laryngeal epidermoid carcinoma of the larynx Hep2 cells. EGCG-induced apoptotic cell death accompanied by a change in the cell cycle. However, EGCG did not result in caspase activation, nor did a cas...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/j.canlet.2009.08.027

    authors： Lee JH,Jeong YJ,Lee SW,Kim D,Oh SJ,Lim HS,Oh HK,Kim SH,Kim WJ,Jung JY

    更新日期：2010-04-01 00:00:00

  • The field bean protease inhibitor has the potential to suppress B16F10 melanoma cell lung metastasis in mice.

    abstract：:Metastasis is a characteristic and fatal feature of human malignancies. Its regulation is therefore of the utmost significance to clinicians. The present study was undertaken to determine whether a legume-derived protease inhibitor (PI) of trypsin/chymotrypsin, the field bean PI (FBPI), also has plasmin inhibitory act...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/s0304-3835(98)00090-1

    authors： Banerji A,Fernandes A,Bane S,Ahire S

    更新日期：1998-07-03 00:00:00

  • HA14-1 sensitizes TNF-alpha-induced apoptosis via inhibition of the NF-kappaB signaling pathway: involvement of reactive oxygen species and JNK.

    abstract：:Nuclear factor-kappa B (NF-kappaB) activation by tumor necrosis factor-alpha (TNF-alpha) attenuates the TNF-alpha-induced apoptosis pathway. Thus, blockage of NF-kappaB activity may improve the anti-cancer activity of TNF-alpha. HA14-1 induces apoptosis in various human cancer cells, and the molecular mechanisms of th...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/j.canlet.2009.11.014

    authors： Moon DO,Kim MO,Kang SH,Choi YH,Park SY,Kim GY

    更新日期：2010-06-01 00:00:00

  • Anti-EGFR and ErbB-2 antibodies attenuate cyclooxygenase-2 expression and cooperatively inhibit survival of human colon cancer cells.

    abstract：:Cyclooxygenase-2 (COX-2) is a transcriptional target and downstream effector of the ErbB-1 (EGFR) and ErbB-2 signaling pathways. We found that anti-EGFR and anti-ErbB-2 antibodies inhibited ERK phosphorylation and downregulated COX-2 protein expression in HCA-7 human colon carcinoma cells. Both antibodies also augment...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/j.canlet.2006.11.020

    authors： Half E,Sun Y,Sinicrope FA

    更新日期：2007-06-28 00:00:00

  • Glioma initiating cells contribute to malignant transformation of host glial cells during tumor tissue remodeling via PDGF signaling.

    abstract：INTRODUCTION:Glioma initiating cells (GICs) play important roles in tumor initiation and progression. However, interactions between tumor cells and host cells of local tumor microenvironment are kept largely unknown. Besides GICs and their progeny cells, whether adjacent normal glial cells contribute to tumorigenesis d...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/j.canlet.2015.05.026

    authors： Chen Y,Wang Z,Dai X,Fei X,Shen Y,Zhang M,Wang A,Li X,Wang Z,Huang Q,Dong J

    更新日期：2015-09-01 00:00:00

  • In vitro induction of specific CD8+ T lymphocytes by tumor-associated antigenic peptides in patients with oral squamous cell carcinoma.

    abstract：:The aim of this study was to clarify candidate peptides for peptide-based specific immunotherapy of patients with oral squamous cell carcinoma (SCC). Thirteen peptides were examined for in vitro induction of peptide-specific CD8(+) T lymphocyte (CD8(+)TL) activity in peripheral blood mononuclear cells from 35 patients...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/j.canlet.2012.02.016

    authors： Toyoshima T,Kumamaru W,Hayashida JN,Moriyama M,Kitamura R,Tanaka H,Yamada A,Itoh K,Nakamura S

    更新日期：2012-09-01 00:00:00

  • How to treat CML patients in the tyrosine kinase inhibitors era? From imatinib standard dose to second generation drugs front-line: unmet needs, pitfalls and advantages.

    abstract：:Imatinib has revolutionized treatment strategies for chronic myeloid leukemia patients: long-term overall survival was reported to be up to 80% at 8 years of follow-up in respondent patients. Despite the straightforward results obtained, it has been estimated a failure rate per year of 2-4%. Several attempts to improv...

    journal_title：Cancer letters

    pub_type： 杂志文章,评审

    doi：10.1016/j.canlet.2012.03.021

    authors： Breccia M,Alimena G

    更新日期：2012-09-28 00:00:00

  • Network-based cancer precision medicine: A new emerging paradigm.

    abstract：:The complex interactions in biological systems have been shown to affect the response to single-targeted therapies which were initially developed under the "reductionist paradigm" of cancer precision medicine. To address these fundamental challenges, great efforts have been dedicated from a network perspective to expl...

    journal_title：Cancer letters

    pub_type： 杂志文章,评审

    doi：10.1016/j.canlet.2019.05.015

    authors： Tan A,Huang H,Zhang P,Li S

    更新日期：2019-08-28 00:00:00

  • Selective hyperexpression of c-jun oncoprotein by glass fiber- and silica-transformed BALB/c-3T3 cells.

    abstract：:Mining and mineral processing are important industries in the United States. A large number of workers are potentially exposed to silica during mining and to glass fibers during manufacturing. There is a concern regarding lung cancer risk among workers exposed to silica and glass fibers. Our previous studies showed th...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/s0304-3835(96)04546-6

    authors： Gao H,Brick J,Ong S,Miller M,Whong WZ,Ong T

    更新日期：1997-01-15 00:00:00

  • Absence of beta-catenin gene mutations in pancreatic duct lesions induced by N-nitrosobis(2-oxopropyl)amine in hamsters.

    abstract：:The involvement of beta-catenin gene alterations in pancreatic duct carcinogenesis induced by N-nitrosobis(2-oxopropyl)amine (BOP) in hamsters was examined by polymerase chain reaction-single strand conformation polymorphism analysis and the expression of beta-catenin protein was examined by immunohistochemistry. No m...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/s0304-3835(01)00406-2

    authors： Kubozoe T,Tsujiuchi T,Murata N,Sasaki Y,Tsunoda T,Konishi Y,Tsutsumi M

    更新日期：2001-07-10 00:00:00

  • Growth inhibition of a prostate tumor by alpha-difluoromethylornithine and by cyclophosphamide.

    abstract：:The effects of the ornithine decarboxylase suicide substrate, difluoromethylornithine (DFMO), and of cyclophosphamide, individually and in combination, on the growth of the R3327MAT-Lu prostate derived tumor were determined. DFMO decreased the growth rate and resulted in a 75% reduction in DNA content compared to the ...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/0304-3835(82)90093-3

    authors： Heston WD,Kadmon D,Fair WR

    更新日期：1982-05-01 00:00:00

  • Effect of glucose administration on hamster liver S9-mediated mutagenesis, metabolism and DNA-binding of benzo[a]pyrene and aflatoxin B1.

    abstract：:Hamster liver S9 prepared from control animals and animals given 30% glucose in drinking water 48 h before killing was used in studies of benzo[a]pyrene (BaP) and aflatoxin (AFB1)-induced mutagenesis, metabolism of BaP and AFB1, and metabolite binding to calf thymus DNA. BAP-induced mutagenesis in Salmonella typhimuri...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/0304-3835(90)90254-u

    authors： Rubano DC,Teel RW,Strother A

    更新日期：1990-07-16 00:00:00

  • Endocrine disrupting compounds and prostate cancer.

    abstract：:Prostate cancer is a major health concern and is treated based on its hormone dependence. Agents that alter hormone action can have substantial biological effects on prostate cancer development and progression. As such, there is significant interest in uncovering the potential effects of endocrine disrupting compound ...

    journal_title：Cancer letters

    pub_type： 杂志文章,评审

    doi：10.1016/j.canlet.2005.10.006

    authors： Hess-Wilson JK,Knudsen KE

    更新日期：2006-09-08 00:00:00

  • Experimental hypoxia is a potent stimulus for radiotracer uptake in vitro: comparison of different tumor cells and primary endothelial cells.

    abstract：:Hypoxia causes upregulation of vascular endothelial growth factor (VEGF) which is a key regulator in tumor angiogenesis and essential for the proliferation of endothelial cells. Endothelial cells have been described to accumulate radiotracers like (18)F-FDG. However, the contribution of radiotracer uptake by endotheli...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/j.canlet.2007.02.016

    authors： Oswald J,Treite F,Haase C,Kampfrath T,Mäding P,Schwenzer B,Bergmann R,Pietzsch J

    更新日期：2007-08-28 00:00:00

  • Targeting Hsp70: A possible therapy for cancer.

    abstract：:In all organisms, heat-shock proteins (HSPs) provide an ancient defense system. These proteins act as molecular chaperones by assisting proper folding and refolding of misfolded proteins and aid in the elimination of old and damaged cells. HSPs include Hsp100, Hsp90, Hsp70, Hsp40, and small HSPs. Through its substrate...

    journal_title：Cancer letters

    pub_type： 杂志文章,评审

    doi：10.1016/j.canlet.2016.01.056

    authors： Kumar S,Stokes J 3rd,Singh UP,Scissum Gunn K,Acharya A,Manne U,Mishra M

    更新日期：2016-04-28 00:00:00

  • Formation of nitroso compounds and mutagens from cinnarizine, ethambutol, piromidic acid, pyridinol carbamate and tiaramide by drug/nitrite interaction.

    abstract：:The formation of nitroso compounds and mutagens from 5 nitrogen-containing drugs (cinnarizine, ethambutol, piromidic acid, pyridinol carbamate and tiaramide) by drug/nitrite interaction was examined. On the reaction of 50 micrometers drug and 500 micrometers nitrite at pH 3.0--3.4 and 37 degrees C for 4 h, the conside...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/0304-3835(82)90075-1

    authors： Takeda Y,Kanaya H

    更新日期：1982-01-01 00:00:00

  • Cisplatin-induced cytotoxicity is blocked by brain-derived neurotrophic factor activation of TrkB signal transduction path in neuroblastoma.

    abstract：:We evaluated the ability of brain-derived neurotrophic factor (BDNF) to decrease the chemosensitivity of neuroblastoma cells to cisplatin. Two cell lines, one derived from SH-SY5Y (SY5Y-TB8) and the other from SK-N-AS (AS-TB8), transfected with a TrkB plasmid were generated, and used to assess the effects of activatio...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/s0304-3835(02)00723-1

    authors： Jaboin J,Hong A,Kim CJ,Thiele CJ

    更新日期：2003-04-10 00:00:00

  • Mutant p53R175H promotes cancer initiation in the pancreas by stabilizing HSP70.

    abstract：:Pancreatic cancer remains a highly lethal malignancy. We have recently shown that simultaneous expression of Kras and mutant Tp53R175H promotes invasive ductal adenocarcinoma from pancreatic ductal cells. We hypothesized specific mutations in TP53 have divergent mechanisms of transforming ductal cells. In order to und...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/j.canlet.2019.03.047

    authors： Polireddy K,Singh K,Pruski M,Jones NC,Manisundaram NV,Ponnela P,Ouellette M,Van Buren G,Younes M,Bynon JS,Dar WA,Bailey JM

    更新日期：2019-07-01 00:00:00

  • Mutagenicity of chamuvaritin: a benzyldihydrochalcone isolated from a medicinal plant.

    abstract：:The mutagenic effects of chamuvaritin, dihydrobenzylchalcone isolated from Uvaria chamae, were investigated using Salmonella typhimurium tester strains TA92, TA94--98, TA100--1535, TA1537 and TA1538. The phytochemical was mutagenic in tester strains TA98 and TA100 and required activation by the hepatic S-9 microsomal ...

    journal_title：Cancer letters

    pub_type： 杂志文章

    doi：10.1016/0304-3835(79)90027-2

    authors： Uwaifo AO,Okorie DA,Bababunmi EA

    更新日期：1979-11-01 00:00:00