Abstract:
PURPOSE:House dust mites (HDMs) are an important source of indoor allergens associated with asthma, rhinitis and atopic dermatitis. Chicken immunoglobulin (Ig) Y is known to be a good alternative to mice and rabbit antibody production. In this study, we produced IgYs specific to HDMs and investigated their IgE immunoreactivities. MATERIALS AND METHODS:Total IgYs were isolated from the yolks of White Leghorn hens immunized with either Dermatophagoides pteronyssinus or D. farinae protein extract. Control antibodies were separated from the yolks of immunized hens with phosphate buffered saline. IgYs specific to HDMs were analyzed using enzyme-linked immunosorbent assay and Western blotting analysis. RESULTS:The concentration of egg IgY specific to D. farinae in an immunized hen increased and the highest achieved was 661.3 ug/mg (per an egg) on day 47, compared with 760 ug/mg IgY specific to D. pteronyssinus on day 16. The D. pteronyssinus or D. farinae-specific IgY was detected by binding of each mite proteins, and their immunoreactivities were elevated dependent of the specific IgY concentration. CONCLUSION:IgY specific to HDMs may be a promising antibody for immunological diagnosis as well as identification of possible resistance relating to HDM allergy.
journal_name
Yonsei Med Jjournal_title
Yonsei medical journalauthors
Lee KE,Han BK,Han JY,Hong JY,Kim MN,Heo WI,Sohn MH,Kim KW,Kim KEdoi
10.3349/ymj.2014.55.4.999subject
Has Abstractpub_date
2014-07-01 00:00:00pages
999-1004issue
4eissn
0513-5796issn
1976-2437pii
201407999journal_volume
55pub_type
杂志文章abstract::House dust mites are the most common cause of allergic sensitization in respiratory allergic patients in the world. Tyrophagus putrescentiae (TP), which was followed by Dermatophagoides farinae (DF) and Dermatophagoides pteronyssinus (DP), has been reported as the third most common house mite in Korea. We previously r...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1998.39.2.109
更新日期:1998-04-01 00:00:00
abstract:PURPOSE:Since 1993, Koreans have used diagnostic criteria set by the Korean Society on Thrombosis and Hemostasis (KSTH) in the diagnosis of overt disseminated intravascular coagulation (DIC). In 2001, the Scientific and Standardization Committee (SCC) of the International Society on Thrombosis and Hemostasis (ISTH) pro...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2007.48.4.595
更新日期:2007-08-31 00:00:00
abstract:PURPOSE:This study aimed to elucidate which echocardiographic criteria at three time points, for cardiac resynchronization therapy (CRT) response, are accurate in discriminating the hierarchical clinical composite end point (HCCEP). MATERIALS AND METHODS:We included 120 patients (age, 66.1±12.6 years; men, 54.2%) who ...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2019.60.1.48
更新日期:2019-01-01 00:00:00
abstract::Silicosis had been considered for decades as an illness with manifestations of lung fibrosis due to inhalation of overconcentrated SiO₂ dust. To the best of our knowledge, studies have yet to report SiO₂ deposits in any other tissues and organs. In the present case, while performing bilateral artificial total hip arth...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2014.55.5.1447
更新日期:2014-09-01 00:00:00
abstract:PURPOSE:Numerous studies have assessed the association of SP110 gene variants with tuberculosis (TB), but the results were inconsistent. Through a comprehensive review and meta-analysis, our study aimed to clarify the nature of genetic risks contributed by 11 polymorphisms for the development of TB. MATERIALS AND METH...
journal_title:Yonsei medical journal
pub_type: 杂志文章,meta分析,评审
doi:10.3349/ymj.2017.58.1.165
更新日期:2017-01-01 00:00:00
abstract:PURPOSE:The purpose of this study is to assess patient attitudes towards anti-glaucoma medication and their association with adherence, visual quality of life, and personality traits. MATERIALS AND METHODS:One hundred and forty-seven glaucoma patients were enrolled this study. The participants were divided into 'pharm...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2010.51.2.261
更新日期:2010-03-01 00:00:00
abstract:PURPOSE:This study aimed to investigate the effects of PIK3CA on the sensitivity of acute B lymphocytic leukemia cells (Nalm-6 cells) to chemotherapy drugs. MATERIALS AND METHODS:Children's normal B lymphocytes and Nalm-6 cells were cultured. Nalm-6 cells were transfected with PIK3CA siRNA (siPIK3CA group) or its nega...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2019.60.2.182
更新日期:2019-02-01 00:00:00
abstract:PURPOSE:Information on the possible role of the ribosomal protein S15a (RPS15a) in gastric cancer is scarce. The aim of this study was to evaluate the impact of RPS15a gene expression on the growth and cell cycle of gastric cancer cells in vitro and in vivo. MATERIALS AND METHODS:RPS15a mRNA expression was examined in...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2018.59.10.1166
更新日期:2018-12-01 00:00:00
abstract:PURPOSE:The objective of this study was to describe hand function in relation with gross motor function and subtype of spastic cerebral palsy and to investigate the relationships among gross motor function, bimanual performance, unimanual capacity and upper limb functional measures in children with spastic cerebral pal...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2013.54.2.516
更新日期:2013-03-01 00:00:00
abstract:PURPOSE:The conventional trocar and cannula method in peritoneal dialysis (PD) catheter insertion has its limitation in clinical setting. The aim of this study was to compare a modified method for percutaneous PD catheter insertion with the conventional method, and demonstrate advantages of the modified method. MATERI...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2015.56.4.981
更新日期:2015-07-01 00:00:00
abstract::Hepatocytes are the primary targets of the hepatitis C virus (HCV). While immunosuppressive roles of HCV core protein have been found in several studies, it remains uncertain whether core protein expressed in hepatocytes rather than in immune cells affects the CD8+ T cell response. In order to transduce genes selectiv...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2005.46.6.827
更新日期:2005-12-31 00:00:00
abstract:PURPOSE:Activation of the innate immune system and chronic low-grade inflammation are thought to be involved in the pathogenesis of atherosclerosis and also thought to be associated with type 2 diabetes and its complications. As a receptor for bacterial lipopolysaccharide and heat-shock proteins, Toll-like receptor 4 (...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2008.49.1.58
更新日期:2008-02-29 00:00:00
abstract:PURPOSE:Rearrangement of the proto-oncogene rearranged during transfection (RET) has been newly identified potential driver mutation in lung adenocarcinoma. Clinically available tyrosine kinase inhibitors (TKIs) target RET kinase activity, which suggests that patients with RET fusion genes may be treatable with a kinas...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2017.58.1.9
更新日期:2017-01-01 00:00:00
abstract::This study proposed an assessment of the correlation of hand bone mineral density measured by dual energy x-ray absorbtiometry (DXA) with the carpo:metacarpal (C:MC) ratio and metacarpal cortical index (CI) in patients with rheumatoid arthritis (RA). The correlation of total hand BMD, CI and C:MC ratio with BMD at oth...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1999.40.5.478
更新日期:1999-10-01 00:00:00
abstract::Although many weakening procedures for the inferior oblique muscle have been advocated, there is some controversy as to the most beneficial procedure for weakening overacting inferior oblique muscles. This study was undertaken to determine if unilateral anterior transposition of the inferior oblique muscle alone could...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2004.45.4.609
更新日期:2004-08-31 00:00:00
abstract:PURPOSE:This study examined 2-year outcome of consecutive therapy using entecavir (ETV) followed by telbivudine (LdT) in subjects with undetectable hepatitis B virus (HBV) DNA level and normal alanine aminotransferase level after the initial 6 months of ETV treatment. MATERIALS AND METHODS:Sixty subjects were randomiz...
journal_title:Yonsei medical journal
pub_type: 杂志文章,随机对照试验
doi:10.3349/ymj.2017.58.3.552
更新日期:2017-05-01 00:00:00
abstract:PURPOSE:The present study investigated the diagnostic accuracy and clinical implications of moderate stenosis (50-69%, Coronary Artery Disease Reporting and Data System, grade 3) on coronary computed tomography angiography (CCTA), compared with invasive coronary angiography (ICA). MATERIALS AND METHODS:Two hundred and...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2018.59.8.937
更新日期:2018-10-01 00:00:00
abstract::Presently, allergy diagnosis and therapy procedures are undergoing a transition phase in which allergen extracts are being step-by-step replaced by molecule-based products. The new developments will allow clinicians to obtain detailed information on sensitization patterns, more accurate interpretation of allergic symp...
journal_title:Yonsei medical journal
pub_type: 杂志文章,评审
doi:10.3349/ymj.2014.55.4.839
更新日期:2014-07-01 00:00:00
abstract::Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominantly inherited syndrome. MEN1 is characterized by the presence of functioning and nonfunctioning tumors or hyperplasia of the pituitary gland, parathyroid glands, and pancreatic islet cells. In addition, MEN1 carriers can have adrenal or thyroid tumors a...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2008.49.4.655
更新日期:2008-08-30 00:00:00
abstract:PURPOSE:The association of interleukin-10 (IL-10) polymorphisms (-1082G/A, -819C/T, -592A/C) and interleukin-6 (IL-6) poly-morphisms (-174G/C) with tuberculosis (TB) risk has been widely reported. However, the results are controversial. To clarify the role of these polymorphisms in TB, we performed a meta-analysis of a...
journal_title:Yonsei medical journal
pub_type: 杂志文章,meta分析
doi:10.3349/ymj.2015.56.5.1274
更新日期:2015-09-01 00:00:00
abstract:PURPOSE:Here, we compared the operative and perioperative outcomes between robot-assisted laparoscopic myomectomy (RALM) and abdominal myomectomy (AM) in patients with large (>10 cm) or heavy myomas (>250 g). MATERIALS AND METHODS:We included 278 patients who underwent multi-port RALM (n=126) or AM (n=151) for large o...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2020.61.12.1054
更新日期:2020-12-01 00:00:00
abstract:PURPOSE:To investigate the relationship between prostate volume and the increased risk for being diagnosed with prostate cancer (PCa) in men with slowly increasing prostate specific antigen (PSA). MATERIALS AND METHODS:A cohort of 1035 men who visited our hospital's health promotion center and were checked for serum P...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2013.54.5.1202
更新日期:2013-09-01 00:00:00
abstract:PURPOSE:Elevated C-reactive protein (CRP) is associated with poor prognosis in several tumor types. The purpose of this study was to investigate serum CRP as a prognostic marker in small cell lung cancer (SCLC). MATERIALS AND METHODS:The pretreatment serum CRP level was measured in 157 newly diagnosed SCLC patients, a...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2012.53.1.111
更新日期:2012-01-01 00:00:00
abstract::The healing pattern of medial and lateral cortical gap in open transverse or short oblique tibial fractures were retrospectively reviewed in 2 groups; In group A, 16 patients were treated by Judet external fixator in rigid mode. In group B, 6 patients were treated in biocompressive mode, which allowed predominantly lo...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1995.36.2.130
更新日期:1995-05-01 00:00:00
abstract::Progeria, also known as Hutchinson-Gilford syndrome, is an extremely rare condition originally described by Hutchinson in 1886. Death results from cardiac complications in the majority of cases and usually occurs at an average age of fourteen years. We recently experienced a patient with progeria who died suddenly aft...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1993.34.4.352
更新日期:1993-12-01 00:00:00
abstract:PURPOSE:Stroke prevention in patients with atrial fibrillation (AF) is influenced by many factors. Using a contemporary registry, we evaluated variables associated with the use of warfarin or direct oral anticoagulants (OACs). MATERIALS AND METHODS:In the prospective multicenter CODE-AF registry, 10529 patients with A...
journal_title:Yonsei medical journal
pub_type: 临床试验,杂志文章,多中心研究
doi:10.3349/ymj.2020.61.2.120
更新日期:2020-02-01 00:00:00
abstract::This study sought to determine the outcomes of posterior lumbar interbody fusion (PLIF), via a unilateral approach, in selected patients who presented with unilateral leg pain and segmental instability of the lumbar spine. Patients with a single level of a herniated disc disease in the lumbar spine, unilateral leg pai...
journal_title:Yonsei medical journal
pub_type: 临床试验,杂志文章
doi:10.3349/ymj.2006.47.3.319
更新日期:2006-06-30 00:00:00
abstract:PURPOSE:Techniques designed to identify differentially expressed genes (DEGs) in tumors have become important in modern pathology. Genefishing technique using the annealing control primer (ACP) system has recently been developed to screen for DEG transcripts. We tried to identify DEGs involved in papillary thyroid canc...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2009.50.1.60
更新日期:2009-02-28 00:00:00
abstract::We have analyzed two (BclI and XbaI) intragenic restriction fragment length polymorphisms (RFLPs) and St14 (DXS52) variable number of tandem repeats (VNTR) by rapid PCR method in 97 unrelated normal subjects. The incidences for positive Bc1I and XbaI polymorphic sites in the Koreans were 81% and 72%, respectively, whi...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1993.34.3.239
更新日期:1993-09-01 00:00:00
abstract:PURPOSE:Owing to the recommendations of the Surviving Sepsis Campaign guidelines, protocol-based resuscitation or goal-directed therapy (GDT) is broadly advocated for the treatment of septic shock. However, the most recently published trials showed no survival benefit from protocol-based resuscitation in septic shock p...
journal_title:Yonsei medical journal
pub_type: 杂志文章,meta分析
doi:10.3349/ymj.2016.57.5.1260
更新日期:2016-09-01 00:00:00