Identification of differentially expressed genes in papillary thyroid cancers.

Abstract:

PURPOSE:Techniques designed to identify differentially expressed genes (DEGs) in tumors have become important in modern pathology. Genefishing technique using the annealing control primer (ACP) system has recently been developed to screen for DEG transcripts. We tried to identify DEGs involved in papillary thyroid cancer (PTC) by using Genefishing technique. MATERIALS AND METHODS:We utilized a new differential display method, designated with Genefishing technique, to analyze DEGs in 21 cases of PTCs. RESULTS:Comparing the gene expression profiles between PTC and normal thyroid, we detected 17 genes that were differentially expressed in PTCs and performed cloning with sequencing in 10 genes. We confirmed the expression patterns of 2 DEGs by RT-PCR assay and identified the same results in 17 out of 21 (81%) PTCs. The 2 DEGs over-expressed in PTCs were identified as DC-STAMP and type I collagen A1. They are novel genes identified first in PTCs. CONCLUSION:We confirmed 2 DEGs in PTCs as DC-STAMP and type I collagen A1 by using Genefishing technique. Although the detailed functions of those 2 genes and their products remain to be determined, the genes will provide insights into mechanisms of carcinogenesis or tumor progression in PTCs.

journal_name

Yonsei Med J

journal_title

Yonsei medical journal

authors

Lee KY,Huang SM,Li S,Kim JM

doi

10.3349/ymj.2009.50.1.60

subject

Has Abstract

pub_date

2009-02-28 00:00:00

pages

60-7

issue

1

eissn

0513-5796

issn

1976-2437

journal_volume

50

pub_type

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