Abstract：
:Cdc6 and Cdt1 initiate DNA replication licensing when cells exit mitosis. In cycling cells, Cdc6 is efficiently degraded from anaphase onwards as a result of APC/C-Cdh1 activity. When APC/C-Cdh1 is switched off again, at the end of G1 phase, Cdc6 could thus re-accumulate, risking the re-licensing of DNA as long as Cdt1 is present. Here, we carefully investigated the dynamics of Cdt1 and Cdc6 in cycling cells. We reveal a novel APC/C-Cdh1-independent degradation pathway that prevents nuclear Cdc6 re-accumulation at the G1-S transition and during S phase. Similar to Cdt1, nuclear clearance of Cdc6 depends on an N-terminal PIP-box and the Cdt2-containing CRL4 complex. When cells reach G2 phase, Cdc6 rapidly re-accumulates but, at this time, Cdt1 is mostly absent and expression of Cdc6 is limited to the cytoplasm. We propose that Cdk1 contributes to the nuclear export of Cdc6 at the S-to-G2 transition. In summary, our results show that different control mechanisms of Cdc6 restrain erroneous licensing of DNA replication during G1, S and G2 phase.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Clijsters L,Wolthuis Rdoi
10.1242/jcs.145862subject
Has Abstractpub_date
2014-03-15 00:00:00pages
1336-45issue
Pt 6eissn
0021-9533issn
1477-9137pii
jcs.145862journal_volume
127pub_type
杂志文章abstract：:The events responsible for repair of DNA interstrand cross-links in mammalian cells, the proteins involved and their interactions with each other are poorly understood. The present study demonstrates that the structural protein nonerythroid alpha spectrin (alphaSpIISigma*), present in normal human cell nuclei, plays a...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00294
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journal_title：Journal of cell science
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.218792
更新日期：2018-12-19 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01000
更新日期：2004-03-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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journal_title：Journal of cell science
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pub_type： 杂志文章
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更新日期：2014-12-01 00:00:00
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journal_title：Journal of cell science
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pub_type： 杂志文章
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更新日期：2005-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1242/jcs.00678
更新日期：2003-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1976-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.248062
更新日期：2020-08-06 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03020
更新日期：2006-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.150888
更新日期：2014-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.067009
更新日期：2011-04-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-08-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.231357
更新日期：2019-10-09 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03217
更新日期：2006-10-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2020-05-14 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
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更新日期：2004-03-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.225557
更新日期：2019-02-01 00:00:00
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pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2015-03-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00065
更新日期：2002-10-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-12-01 00:00:00
