Identification of triploidy by DA/DAPI staining of trophoblastic interphase nuclei.

Abstract:

:Combined staining of non-cultured interphase nuclei from hydatidiform moles with distamycin A and DAPI resulted in the presence of specific stained interphase bodies. A mean number of six interphase bodies was observed in moles with a diploid karyotype and a mean number of nine interphase bodies was observed in triploid moles. It is concluded that the interphase bodies are primarily due to specific staining of the large heterochromatic areas on chromosomes 1, 9 and 16. The method may permit a rapid measurement of the ploidy of non-cultured hydatidiform moles and identify partial, triploid moles.

journal_name

Placenta

journal_title

Placenta

authors

Tommerup N,Vejerslev LO

doi

10.1016/s0143-4004(85)80046-1

subject

Has Abstract

pub_date

1985-07-01 00:00:00

pages

363-7

issue

4

eissn

0143-4004

issn

1532-3102

pii

S0143-4004(85)80046-1

journal_volume

6

pub_type

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