Abstract:
:Diet-induced obesity (DIO) predisposes individuals to insulin resistance, and adipose tissue has a major role in the disease. Insulin resistance can be induced in cultured adipocytes by a variety of treatments, but what aspects of the in vivo responses are captured by these models remains unknown. We use global RNA sequencing to investigate changes induced by TNF-α, hypoxia, dexamethasone, high insulin, and a combination of TNF-α and hypoxia, comparing the results to the changes in white adipose tissue from DIO mice. We found that different in vitro models capture distinct features of DIO adipose insulin resistance, and a combined treatment of TNF-α and hypoxia is most able to mimic the in vivo changes. Using genome-wide DNase I hypersensitivity followed by sequencing, we further examined the transcriptional regulation of TNF-α-induced insulin resistance, and we found that C/EPBβ is a potential key regulator of adipose insulin resistance.
journal_name
Cell Repjournal_title
Cell reportsauthors
Lo KA,Labadorf A,Kennedy NJ,Han MS,Yap YS,Matthews B,Xin X,Sun L,Davis RJ,Lodish HF,Fraenkel Edoi
10.1016/j.celrep.2013.08.039subject
Has Abstractpub_date
2013-10-17 00:00:00pages
259-70issue
1issn
2211-1247pii
S2211-1247(13)00480-4journal_volume
5pub_type
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