Abstract:
:Inflammation and vascular injury triggered by ischemia/reperfusion (I/R) represent a leading cause of morbidity and mortality in a number of clinical settings. Wnt and its homolog partners R-spondins, in addition to regulating embryonic development have recently been demonstrated to serve as wound-healing agents in inflammation-associated conditions. Here we ask whether R-spondins could prevent inflammation-associated tissue damage in ischemic disorders and thus investigate the role of R-spondin3 (R-spo3) in a mouse model of mesenteric I/R. We demonstrate that R-spo3 ameliorates mesenteric I/R-induced local intestinal as well as remote lung damage by suppressing local and systemic cytokine response and deposition of IgM and complement in intestinal tissues. We also show that decreased inflammatory response is accompanied by tightening of endothelial cell junctions and reduction in vascular leakage. We conclude that R-spo3 stabilizes endothelial junctions and inhibits vascular leakage during I/R and thereby mitigates the inflammatory events and associated tissue damage. Our findings uniquely demonstrate a protective effect of R-spo3 in I/R-related tissue injury and suggest a mechanism by which it may have these effects.
journal_name
Proc Natl Acad Sci U S Aauthors
Kannan L,Kis-Toth K,Yoshiya K,Thai TH,Sehrawat S,Mayadas TN,Dalle Lucca JJ,Tsokos GCdoi
10.1073/pnas.1309393110subject
Has Abstractpub_date
2013-08-27 00:00:00pages
14348-53issue
35eissn
0027-8424issn
1091-6490pii
1309393110journal_volume
110pub_type
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