Abstract:
:Most human cancers are aneuploid and have chromosomal instability, which contrasts to the inability of human cells to normally tolerate aneuploidy. Noting that aneuploidy in human breast cancer correlates with increased expression levels of the Mps1 checkpoint gene, we investigated whether these high levels of Mps1 contribute to the ability of breast cancer cells to tolerate this aneuploidy. Reducing Mps1 levels in cultured human breast cancer cells by RNAi resulted in aberrant mitoses, induction of apoptosis, and decreased ability of human breast cancer cells to grow as xenografts in nude mice. Remarkably, breast cancer cells that survive reductions in levels of Mps1 have relatively less aneuploidy, as measured by copies of specific chromosomes, compared with cells that have constitutively high levels of Mps1. Thus, high levels of Mps1 in breast cancer cells likely contribute to these cells tolerating aneuploidy.
journal_name
Proc Natl Acad Sci U S Aauthors
Daniel J,Coulter J,Woo JH,Wilsbach K,Gabrielson Edoi
10.1073/pnas.1007645108subject
Has Abstractpub_date
2011-03-29 00:00:00pages
5384-9issue
13eissn
0027-8424issn
1091-6490pii
1007645108journal_volume
108pub_type
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