Abstract：
:A growing number of studies have examined transcriptional responses to sex steroids along the hypothalamic-pituitary-gonadal axis in teleost fishes. However, data are lacking on the molecular cascades that underlie progesterone signaling. The objective of this study was to characterize the transcriptional response in the ovary of fathead minnows (Pimephales promelas) in response to progesterone (P4). Fathead minnow ovaries were exposed in vitro to 500 ng P4/L. Germinal vesicle migration and breakdown (GVBD) was observed and microarrays were used to identify gene cascades affected by P4. Microarray analysis identified 1702 differentially expressed transcripts after P4 treatment. Functional enrichment analysis revealed that transcripts involved in the molecular functions of protein serine/threonine kinase activity, ATP binding, and activity of calcium channels were increased after P4 treatment. There was an overwhelming decrease in levels of transcripts of genes that are structural constituents of ribosomes with P4 treatment. There was also evidence for gene expression changes in steroid and maturation-related transcripts. Pathway analyses identified cell cycle regulation, insulin action, hedgehog, and B cell activation as pathways containing an over-representation of highly regulated transcripts. Significant regulatory sub-networks of P4-mediated transcripts included genes regulated by tumor protein p53 and E2F transcription factor 1. These data provide novel insight into the molecular signaling cascades that underlie P4-signaling in the ovary and identify genes and processes that may indicate premature GVBD due to environmental pollutants that mimic progestins.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Garcia-Reyero N,Martyniuk CJ,Kroll KJ,Escalon BL,Spade DJ,Denslow NDdoi
10.1016/j.ygcen.2013.06.008subject
Has Abstractpub_date
2013-10-01 00:00:00pages
159-69eissn
0016-6480issn
1095-6840pii
S0016-6480(13)00275-Xjournal_volume
192pub_type
杂志文章abstract：:We investigated the seasonal and daily variation in plasma glucose levels in response to stress in captive wild starlings. Starlings were captured from the wild during the winter, held on short days (11L:13D, mimicking winter), and then shifted to long days (19L:5D, mimicking summer). Birds were maintained on long day...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2000.7492
更新日期：2000-07-01 00:00:00
abstract：:Oligonucleotide probes were synthesized for the mRNAs of a pair of tilapia prolactins (tPRL177 and tPRL188) and growth hormone (tGH) based on cDNAs for the hormones of Oreochromis niloticus and amino acid sequences for the hormones of O. mossambicus. The three 45mer probes were labeled with 35S for hybridization studi...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1993.1010
更新日期：1993-01-01 00:00:00
abstract：:Growth hormone (GH) and insulin-like growth factor-I (IGF-I) play major roles in the endocrine regulation of fish growth, but their interdependency and mode of action has not been well elucidated. The GH-IGF-I system is essential for normal vertebral growth in mouse, but this has not been studied in fish. To study the...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2004.12.005
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.02.010
更新日期：2010-05-15 00:00:00
abstract：:As an alternative to radioimmunoassays, a simple, highly sensitive and quick enzymeimmunoassay (EIA) for determination of testosterone in blood plasma of yaks on microtitreplates using second antibody coating technique and testosterone-horseradish peroxidase as a label has been developed for the first time. The wells ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.07.007
更新日期：2008-09-15 00:00:00
abstract：:Insulin receptor downregulation in the isolated hepatocytes of lamprey (Lampetra fluviatilis) was studied at the ambient temperature for this species. Preincubation of hepatocytes with 10(-9)-10(-8) M unlabeled insulin decreased insulin binding capacity to 43, 37, and 34% at 4, 15, and 25 degrees, respectively. Preinc...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1995.1126
更新日期：1995-10-01 00:00:00
abstract：:The Harderian gland of the lizard Podarcis sicula sicula (Raf) contains connective tissue type mast cells whose numbers vary during the year showing two peaks, one in spring the other in winter. No sex differences are found throughout the year. Thermal and photoperiodic manipulations indicate that only temperature inf...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1997.6925
更新日期：1997-09-01 00:00:00
abstract：:Anthropogenic emissions of carbon dioxide in the atmosphere have generated rapid variations in atmospheric composition which drives major climate changes. Climate change related effects include changes in physico-chemical proprieties of sea and freshwater, such as variations in water temperature, salinity, pH/pCO2 and...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2020.113439
更新日期：2020-05-15 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2017.07.006
更新日期：2018-01-15 00:00:00
abstract：:Insulin-like growth factor-1 (IGF-1) is a member of the vertebrate insulin/insulin-like growth factor/relaxin gene family necessary for growth, reproduction, and survival at both the cellular and organismal level. Its sequence, protein structure, and function have been characterized in mammals, birds, and fish; howeve...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.04.022
更新日期：2012-08-01 00:00:00
abstract：:Secretin (Sct), traditionally a gastrointestinal hormone backed by a century long research, is now beginning to be recognized also as a neuroactive peptide. Substantiation by recent evidence on the functional role of Sct in various regions of the brain, especially on its potential neurosecretion from the posterior pit...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2012.11.017
更新日期：2013-01-15 00:00:00
abstract：:The class Chondrichthyes, that includes Elasmobranchii and Holocephali, is a diverse group of fish occupying a key position at the base of vertebrate evolution. Their evolutionary success is greatly attributed to their wide range of reproductive strategies controlled by different endocrine mechanics. As in other verte...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2013.05.021
更新日期：2013-10-01 00:00:00
abstract：:Plasma glucagon levels in 11 different teleosts and in dogfish were evaluated using an adapted classical mammalian radioimmunoassay (RIA). The values obtained are considered relative; sensitivity was inferior to the original method, but the validation experiment results are acceptable. The mean value of the coefficien...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(86)90131-0
更新日期：1986-09-01 00:00:00
abstract：:Songbirds possess a specialized network of brain nuclei mediating song learning and production, the song system. In many species, the nuclei in this circuit are larger in volume in males housed on long photoperiods than in males housed on short photoperiods. Exposure to long days initially leads to increases in circul...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1996.6829
更新日期：1997-02-01 00:00:00
abstract：:This experiment was performed on two groups of male Japanese quail. One had been maintained in our laboratory as a closed colony (S-group), and the other had been obtained from a commercial source (R-group). Different responses of gonadal function were found between two groups following either testosterone treatment o...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(85)90390-9
更新日期：1985-09-01 00:00:00
abstract：:Changes in plasma cortisol and glucose concentration were studied in carp during acclimation from fresh water (FW) to 1.5% salt water and vice versa. There was an increase in cortisol and glucose concentration during acclimation from FW to salt water which lasted for several days. Reacclimation to FW did not cause cle...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(84)90155-2
更新日期：1984-06-01 00:00:00
abstract：:Arsenic contaminates water surface and groundwater worldwide. Several studies have suggested that arsenic acts as an endocrine disruptor in mammalian and non-mammalian species, although its chronic effect during development remains largely unknown. To address this question, life cycle exposures to 0, 0.3 and 0.8ppm of...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2015.09.015
更新日期：2016-08-01 00:00:00
abstract：:The presence of specific, saturable, high-affinity gonadotropin receptors was demonstrated in membrane preparations from preovulatory ovarian follicles of amago salmon (Oncorhynchus rhodurus), including intact follicles, isolated thecal layers, and isolated granulosa cells. Optimum conditions for the binding study usi...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90177-3
更新日期：1988-10-01 00:00:00
abstract：:Appetite and reproduction are closely related functions that are both regulated by brain hormones. Appetite stimulators include orexin and neuropeptide Y (NPY), and reproductive hormones include gonadotropin-releasing hormone (GnRH), gonadotropin-inhibitory hormone (GnIH), kisspeptin, and neurokinin B (NKB). GnRH stim...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2019.06.011
更新日期：2019-10-01 00:00:00
abstract：:Serum levels of 17 beta-hydroxy-4-androstene-3,11-dione (11-KT) and testosterone in the protogynous black sea bass, Centropristis striatus fluctuate annually, correlated with the breeding season. Although serum 11-KT levels in both males and females exhibit seasonality, serum estradiol-17 beta concentrations cycle ann...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(91)90297-j
更新日期：1991-04-01 00:00:00
abstract：:Uptake of L-thyroxine (T4) by isolated trout hepatocytes was characterized after 1-min incubation of cells in a Hanks' balanced salts medium containing glucose and [125I]T4. Centrifugation through silicone oil and glycine buffer (pH 10.5) was used to separate cells from the medium and minimize extracellular binding of...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1993.1057
更新日期：1993-04-01 00:00:00
abstract：:Cholecystokinin (CCK) and gastrin-releasing peptide (GRP) are gastrointestinal peptides thought to be important regulators of intake and digestion of food in vertebrates. In this study, pre- and postprandial plasma levels of CCK and GRP were measured in rainbow trout (Oncorhynchus mykiss) by the establishment of homol...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2006.02.016
更新日期：2006-09-01 00:00:00
abstract：:Four genomes and two transcriptomes from six Chelicerate species were analyzed for the presence of neuropeptide and neurohormone precursors and their GPCRs. The genome from the spider Stegodyphus mimosarum yielded 87 neuropeptide precursors and 101 neuropeptide GPCRs. High neuropeptide transcripts were also found in t...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2015.07.014
更新日期：2016-11-09 00:00:00
abstract：:The present study examined plasma concentrations of insulin-like growth factor (IGF)-I, IGF-II and IGF-binding proteins (IGFBPs) during posthatch growth and development in chickens. Three distinct proteins which bound 125I-IGF-I were observed irrespective of age or sex, these having apparent molecular weights of 22, 2...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1997.6898
更新日期：1997-07-01 00:00:00
abstract：:By mapping the distribution of cholecystokinin (CCK) receptor types onto an established phylogenetic hypothesis of vertebrate relationships, we tested two hypothesis about the evolution of CCK receptors: (1) A single CCK receptor type, CCK-X, is the ancestral receptor, while CCK-A and CCK-B receptors represent derived...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1996.0047
更新日期：1996-04-01 00:00:00
abstract：:The widespread use of P-nitrophenol (PNP) as a raw material in pesticides, medicines and dyes has led to environmental pollution. PNP is a well-known endocrine disruptor in mammals and quails. This study investigated the effects of long-term PNP exposure on the testicular development and semen quality of roosters. Pub...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2020.113656
更新日期：2021-01-15 00:00:00
abstract：:Our previous study showed that docosahexaenoic acid (DHA) plays an important role in decreasing lipid accumulation by inducing apoptosis of the adipocytes in grass carp. However, the mechanism involved remains unclear. DHA has been reported as the natural ligand of PPARγ. The present study aimed to assess whether PPAR...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2018.05.020
更新日期：2018-09-15 00:00:00
abstract：:Prolonged or frequent secretion of adrenal glucocorticoids in response to aversive stimuli can negatively impact reproduction. Because female southern white rhinoceros (Ceratotherium simum simum) reproduce poorly in captivity, we compared fecal corticosterone metabolite concentrations among parous, nulliparous, and ad...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.02.010
更新日期：2011-05-01 00:00:00
abstract：:To understand the effect of testosterone on sex differentiation, the quantities of testosterone (T) and estradiol-17beta (E2) in developing eggs of medaka (Oryzias latipes) were measured by radioimmunoassay, and the influence on sex differentiation of treating embryos with exogenous androgens was also examined. Endoge...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2005.07.003
更新日期：2006-01-01 00:00:00
abstract：:Our previous study showed the seasonal variations in expression of vasotocin (VT) and isotocin (IT) genes in preoptic magnocellular neurons of female masu salmon (Oncorhynchus masou). The changes in the level of VT mRNA were coincident with those in plasma testosterone and estradiol levels. In the present study, gener...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1999.7344
更新日期：1999-10-01 00:00:00
