Insulin receptor downregulation in isolated hepatocytes of river lamprey (Lampetra fluviatilis).


:Insulin receptor downregulation in the isolated hepatocytes of lamprey (Lampetra fluviatilis) was studied at the ambient temperature for this species. Preincubation of hepatocytes with 10(-9)-10(-8) M unlabeled insulin decreased insulin binding capacity to 43, 37, and 34% at 4, 15, and 25 degrees, respectively. Preincubation of hepatocytes in the presence of 10(-10) M unlabeled insulin had no effect on the 125I-insulin binding. The maximal decrease in the 125I-insulin binding was reached after 10 min of preincubation and was then maintained at a constant level for 2 hr. Competitive binding assays demonstrated that preincubation with unlabeled insulin resulted in a 45% decline in the number of binding sites. In rat adipocytes and frog hepatocytes used for the comparison, preincubation with insulin caused a 65% decrease in cell-surface receptors, while intracellular (internalized receptors) increased proportionally. In contrast, in the lamprey hepatocytes both cell-surface and intracellular receptors decreased. We conclude that insulin receptors of the lamprey hepatocytes could be down-regulated at the ambient temperature for the species and at the physiological concentrations of insulin (10(-9)-10(-8) M).


Gen Comp Endocrinol


Leibush BN,Lappova YL




Has Abstract


1995-10-01 00:00:00














  • The significance of cortisol for osmoregulation in carp (Cyprinus carpio) and tilapia (Sarotherodon mossambicus).

    abstract::Changes in plasma cortisol and glucose concentration were studied in carp during acclimation from fresh water (FW) to 1.5% salt water and vice versa. There was an increase in cortisol and glucose concentration during acclimation from FW to salt water which lasted for several days. Reacclimation to FW did not cause cle...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Abo Hegab S,Hanke W

    更新日期:1984-06-01 00:00:00

  • Control of vascular smooth muscle cell growth in fowl.

    abstract::In adult domestic fowl, angiotensin (ANG) receptors are present in the vascular smooth muscles (VSM) and in the endothelium, mediating vasorelaxation via endothelium-derived relaxing factor/cGMP. ANG II-induced relaxation is minor in chicks and becomes more marked as they mature but diminishes in adult birds, whereas ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Shimada T,Fabian M,Yan HQ,Nishimura H

    更新日期:1998-10-01 00:00:00

  • Hormone-sensitive lipase in yellow catfish Pelteobagrus fulvidraco: molecular characterization, mRNA tissue expression and transcriptional regulation by leptin in vivo and in vitro.

    abstract::Hormone-sensitive lipase (hsl) plays a pivotal role in regulation of lipolysis in mammals, but information is very scarce about its gene structure and function in fish. In this study, two distinct hsl cDNAs, designated hsl1 and hsl2, were firstly isolated and characterized from yellow catfish Pelteobagrus fulvidraco. ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Chen QL,Luo Z,Song YF,Wu K,Huang C,Pan YX,Zhu QL

    更新日期:2014-09-15 00:00:00

  • Brain arginine vasotocin and isotocin in breeding female three-spined sticklebacks (Gasterosteus aculeatus): the presence of male and egg deposition.

    abstract::Arginine vasotocin (AVT) and isotocin (IT) are fish hypothalamic nonapeptides involved in numerous social and reproductive behaviors. Vasotocinergic and isotocinergic fibers project to different brain areas where peptides act as neurotransmitters and/or neuromodulators. In this study, we measured whole brain levels of...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Kulczykowska E,Kleszczyńska A

    更新日期:2014-08-01 00:00:00

  • Isolation and identification of testosterone from the serum and testes of the American lobster (Homarus americanus).

    abstract::Testosterone was isolated and quantified from male lobster serum and testes by solvent extraction, sequential thin-layer chromatography, and high-performance liquid chromatography. The identity of the isolated steroid was established by its isopolarity and isomorphicity with authentic radiolabeled testosterone and its...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Burns BG,Sangalang GB,Freeman HC,McMenemy M

    更新日期:1984-06-01 00:00:00

  • Differential expression of PTHrP and its receptor in pituitary gland and gills in estradiol-treated gilthead sea bream (Sparus auratus, L.).

    abstract::In the gilthead sea bream (Sparus auratus) 17β-estradiol (E₂) plays an important role in the synthesis of vitellogenin. During vitellogenesis, vitellogenin as a nutritional precursor protein is loaded with calcium, which requires elevated plasma calcium levels. This is accomplished via E₂-dependent processes. Reports ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Bevelander GS,Guerreiro PM,Spanings T,Flik G

    更新日期:2011-08-01 00:00:00

  • Selective elimination/RNAi silencing of FMRF-related peptides and their receptors decreases the locomotor activity in Drosophila melanogaster.

    abstract::Five neuropeptide genes are classified in the FMRF-related (FaRP) group: the Fmrf, dromyosuppressin (Dms), drosulfakinin (Dsk), neuropeptide F (npf) and short neuropeptide F (sNPF) genes coding for 8, 1, 2, 1 and 4 peptides, respectively. In order to compare their effects on the locomotor activity of Drosophila adults...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Kiss B,Szlanka T,Zvara Á,Žurovec M,Sery M,Kakaš Š,Ramasz B,Hegedűs Z,Lukacsovich T,Puskás L,Fónagy A,Kiss I

    更新日期:2013-09-15 00:00:00

  • Histological and immunocytochemical study of the endocrine pancreas of the lizard Podarcis hispanica Steindachner, 1870 (Lacertidae).

    abstract::The endocrine pancreas of the lizard Podarcis hispanica is described using light and electron microscopy. The endocrine pancreas of this reptile is located throughout the spleen side of the organ and consists of islet-like structures, small groups of two to five cells, and single scattered endocrine cells. The endocri...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: López J,Echevarría M,Vázquez JJ

    更新日期:1988-08-01 00:00:00

  • Amphibian melanotrophs as a model to analyze the secretory plasticity of endocrine cells.

    abstract::Precise regulation of hormone secretion from endocrine cells is of critical importance for the maintenance of animal homeostasis. This is partly enabled through the ability of endocrine cells to adapt dynamically their secretory activity to the physiological demands through complex functional changes. Such a secretory...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章,评审


    authors: Peinado JR,Castaño JP,Vázquez-Martínez R,Anouar Y,Tonon MC,Vaudry H,Gracia-Navarro F,Malagón MM

    更新日期:2002-03-01 00:00:00

  • Transfer of tilapia (Oreochromis niloticus) to a hyperosmotic environment is associated with sustained expression of prolactin receptor in intestine, gill, and kidney.

    abstract::Expression of the tilapia prolactin receptor (tiPRL-R) has been characterized in the intestine of Oreochromis niloticus and the levels of both tiPRL-R transcripts and tiPRL binding sites have been further analyzed in this organ, as well as in gill and kidney, during adaptation of tilapia to a hyperosmotic environment....

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Sandra O,Le Rouzic P,Rentier-Delrue F,Prunet P

    更新日期:2001-09-01 00:00:00

  • Impairment of microvascular angiogenesis is associated with delay in prostatic development in rat offspring of maternal protein malnutrition.

    abstract::Experimental data demonstrated the negative impact of maternal protein malnutrition (MPM) on rat prostate development, but the mechanism behind the impairment of prostate growth has not been well understood. Male Sprague Dawley rats, borned to dams fed a normal protein diet (CTR group, 17% protein diet), were compared...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Colombelli KT,Santos SAA,Camargo ACL,Constantino FB,Barquilha CN,Rinaldi JC,Felisbino SL,Justulin LA

    更新日期:2017-05-15 00:00:00

  • Increase in ovarian alpha-inhibin gene expression and plasma immunoreactive inhibin level is correlated with a decrease in ovulation rate in the domestic hen.

    abstract::This study was designed to examine the relationship among ovulation rate, inhibin alpha-subunit gene expression, and plasma immunoreactive inhibin levels between hens laying at a high ovulation rate and hens laying at a low ovulation rate. A porcine inhibin alpha-subunit cDNA was used to examine the expression of inhi...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Wang SY,Johnson PA

    更新日期:1993-07-01 00:00:00

  • Multiple forms of glucagon and somatostatin isolated from the intestine of the southern-hemisphere lamprey Geotria australis.

    abstract::Current views on Agnathan phylogeny favor the hypothesis that the genera of holarctic lampreys belong to a single family (Petromyzontidae) and form an interrelated progression in which Petromyzon is near to Ichthomyzon at the base of the phylogenetic tree and Lampetra is the most derived. A stock similar to that of co...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Wang Y,Nielsen PF,Youson JH,Potter IC,Conlon JM

    更新日期:1999-02-01 00:00:00

  • Seasonal changes in androgen receptor mRNA in the brain of the white-crowned sparrow.

    abstract::In songbirds, neurons that regulate learned song behavior undergo extensive seasonal plasticity in their number and size in relation to the bird's reproductive status. Seasonal plasticity of these brain regions is primarily regulated by changes in circulating concentrations of testosterone. Androgen receptors are pres...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Fraley GS,Steiner RA,Lent KL,Brenowitz EA

    更新日期:2010-03-01 00:00:00

  • Regulation of growth hormone (GH) receptor (GHR1 and GHR2) mRNA level by GH and metabolic hormones in primary cultured tilapia hepatocytes.

    abstract::Growth hormone (GH) regulates essential physiological functions in teleost fishes, including growth, metabolism, and osmoregulation. Recent studies have identified two clades of putative receptors for GH (GHR1 clade and GHR2 clade) in fishes, both of which are highly expressed in the liver. Moreover, the liver is an i...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Pierce AL,Breves JP,Moriyama S,Uchida K,Grau EG

    更新日期:2012-10-01 00:00:00

  • Seasonal gonadal recrudescence in song sparrows: response to temperature cues.

    abstract::Day length predicts changing of seasons at mid-latitudes, but additional environmental cues (e.g., temperature, rainfall) give more precise information about timing of food peaks critical for offspring survival. We tested the effects of temperature on seasonal reproductive development of male song sparrows (Melospiza ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Perfito N,Meddle SL,Tramontin AD,Sharp PJ,Wingfield JC

    更新日期:2005-09-01 00:00:00

  • Evolution of insulin-like growth factor (IGF) function: production and characterization of recombinant hagfish IGF.

    abstract::While there is considerable structural evidence that insulin-like growth factors (IGFs) share a long evolutionary history, little is known about the conservation of IGF function. In order to address this, we have made recombinant hagfish IGF, hence allowing characterization of an IGF from a representative of the primi...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Upton Z,Francis GL,Chan SJ,Steiner DF,Wallace JC,Ballard FJ

    更新日期:1997-01-01 00:00:00

  • Reproduction in worker honey bees is associated with low juvenile hormone titers and rates of biosynthesis.

    abstract::Three experiments were performed to determine the role of juvenile hormone (JH) in worker reproduction in queenless colonies of honey bees. In Experiment 1, egg-laying workers had low hemolymph titers of JH, as did bees engaged in brood care, while foragers had significantly higher titers. Experiment 2 confirmed these...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Robinson GE,Strambi C,Strambi A,Huang ZY

    更新日期:1992-09-01 00:00:00

  • Different actions of ecdysis-triggering hormone on the brain and ventral nerve cord of the hornworm, Manduca sexta.

    abstract::Ecdysis, or the shedding of the old cuticle, depends on coordinated stereotyped behaviors, regulated by a number of neuropeptides. In the hornworm, Manduca sexta, two neuropeptides interact, namely ecdysis-triggering hormone (ETH) and eclosion hormone. We looked at the effects of ETH in vivo and in vitro, on the brain...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Asuncion-Uchi M,El Shawa H,Martin T,Fuse M

    更新日期:2010-03-01 00:00:00

  • In vitro effects of ACTH on interrenal corticosteroidogenesis during early larval development in rainbow trout.

    abstract::Interrenal tissue from embryonic and larval rainbow trout (Oncorhynchus mykiss) was cultured in vitro and exposed to various doses of adrenocorticotropin (ACTH) to document the ontogeny of interrenal responsiveness to tropic stimulation. Resting and acute stress-induced changes in whole-body cortisol levels in vivo we...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Barry TP,Ochiai M,Malison JA

    更新日期:1995-09-01 00:00:00

  • Effects of corticosteroids and protein synthesis inhibitors on activities of oxidative enzymes in a bony fish, Anabas testudineus (Bloch).

    abstract::The activities of oxidative enzymes of hepatic mitochondria of Anabas testudineus increased following exogenous administration of hydrocortisone and corticosterone. The inhibitors of protein synthesis prevented the hormone-mediated increase of hepatic enzymes, suggesting that corticosteroids regulate the oxidative met...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Ignatius J,Oommen OV

    更新日期:1990-05-01 00:00:00

  • Comparison of recombinant barramundi and human insulin-like growth factor (IGF)-I in juvenile barramundi (Lates calcarifer): in vivo metabolic effects, association with circulating IGF-binding proteins, and tissue localisation.

    abstract::The in vivo actions of human and fish insulin-like growth factor (IGF)-I have been compared to extend the understanding of the metabolism of IGFs in fish and to identify potential differences in their actions. The effects of acute administration of these proteins on the incorporation of glucose into muscle glycogen an...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Degger B,Upton Z,Soole K,Collet C,Richardson N

    更新日期:2000-03-01 00:00:00

  • Immunohistochemical localization of beta-endorphin-like material in the urodele and anuran amphibian tissues.

    abstract::In the present study, we have localized for the first time beta-endorphin (beta-EP)-like material in the adult and larval urodele and anuran tissues using immunohistochemical techniques. In the adult Notophthalmus viridescens and Ambystoma mexicanum, strong immunoreactivity to beta-EP antisera was observed in the regi...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Vethamany-Globus S

    更新日期:1989-08-01 00:00:00

  • Development and control of the circadian pacemaker for melatonin release in the chicken pineal gland.

    abstract:UNLABELLED:Melatonin (MT) release from explanted pineal glands of 3- to 20-week-old chicken was investigated in a 5-day perifusion system. Both the chicken and the explanted glands were exposed to various environmental lighting regimens. OBSERVATIONS:(1) The explanted chicken pineal is sensitive to direct light. Conti...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Csernus V,Ghosh M,Mess B

    更新日期:1998-04-01 00:00:00

  • Localization of diversified relaxin gene transcripts in the brain of eels.

    abstract::Relaxin 3 (RLN3) is a newly-discovered member of the insulin superfamily. We isolated three RLN3-like cDNAs from the brain of the Japanese eel (Anguilla japonica). The deduced amino acid sequences of the RLN3-like cDNAs contained the two-chain structure common to relaxin including a RXXXRXXI/V motif in the B-chain. Ph...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Hu GB,Kusakabe M,Takei Y

    更新日期:2011-07-01 00:00:00

  • Behavioral insensitivity to testosterone: why and how does testosterone alter paternal and aggressive behavior in some avian species but not others?

    abstract::Considerable research has been conducted on the interrelationships of the steroid hormone testosterone and reproductive behavior in seasonally breeding birds. In species in which males provide paternal care, males experience a peak in testosterone secretion that coincides with territory establishment and pair bonding,...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章,评审


    authors: Lynn SE

    更新日期:2008-07-01 00:00:00

  • Changes in plasma cortisol during stress and smoltification in coho salmon, Oncorhynchus kisutch.

    abstract::The cortisol stress response in juvenile coho salmon, Oncorhynchus kisutch, considered as the difference between resting and peak poststress cortisol levels, increased from 80 ng/ml in March to 166 ng/ml in July, the period when smoltification normally occurs. Resting plasma cortisol levels also continually increased ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Barton BA,Schreck CB,Ewing RD,Hemmingsen AR,Patiño R

    更新日期:1985-09-01 00:00:00

  • Sight of a predator can stimulate a corticosterone response in the great tit (Parus major).

    abstract::The corticosterone response to the sight of a natural predator was investigated in free-living and captive great tits (Parus major). Free-living great tits responded to the sight of a stuffed, slowly moving Tengmalm's owl, a major predator of great tits, with warning calls and a change in behaviour around a feeder. Gr...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Cockrem JF,Silverin B

    更新日期:2002-02-01 00:00:00

  • Mutual regulation by GnRH and kisspeptin of their receptor expression and its impact on the gene expression of gonadotropin subunits.

    abstract::Hypothalamic kisspeptin plays a pivotal role in the regulation of the hypothalamic-pituitary-gonadal axis by stimulating gonadotropin-releasing hormone (GnRH) release into the portal circulation, with the subsequent release of gonadotropins. Kisspeptin and its receptor, the kisspeptin 1 receptor (Kiss1R), are also exp...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Mijiddorj T,Kanasaki H,Sukhbaatar U,Oride A,Hara T,Kyo S

    更新日期:2017-05-15 00:00:00

  • Number of mast cells in the Harderian gland of the lizard Podarcis sicula sicula (Raf): the annual cycle and its relation to environmental factors and estradiol administration.

    abstract::The Harderian gland of the lizard Podarcis sicula sicula (Raf) contains connective tissue type mast cells whose numbers vary during the year showing two peaks, one in spring the other in winter. No sex differences are found throughout the year. Thermal and photoperiodic manipulations indicate that only temperature inf...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Vitiello II,Baccari GC,Di Matteo L,Rusciani A,Chieffi P,Minucci S

    更新日期:1997-09-01 00:00:00