Insulin receptor downregulation in isolated hepatocytes of river lamprey (Lampetra fluviatilis).

Abstract:

:Insulin receptor downregulation in the isolated hepatocytes of lamprey (Lampetra fluviatilis) was studied at the ambient temperature for this species. Preincubation of hepatocytes with 10(-9)-10(-8) M unlabeled insulin decreased insulin binding capacity to 43, 37, and 34% at 4, 15, and 25 degrees, respectively. Preincubation of hepatocytes in the presence of 10(-10) M unlabeled insulin had no effect on the 125I-insulin binding. The maximal decrease in the 125I-insulin binding was reached after 10 min of preincubation and was then maintained at a constant level for 2 hr. Competitive binding assays demonstrated that preincubation with unlabeled insulin resulted in a 45% decline in the number of binding sites. In rat adipocytes and frog hepatocytes used for the comparison, preincubation with insulin caused a 65% decrease in cell-surface receptors, while intracellular (internalized receptors) increased proportionally. In contrast, in the lamprey hepatocytes both cell-surface and intracellular receptors decreased. We conclude that insulin receptors of the lamprey hepatocytes could be down-regulated at the ambient temperature for the species and at the physiological concentrations of insulin (10(-9)-10(-8) M).

journal_name

Gen Comp Endocrinol

authors

Leibush BN,Lappova YL

doi

10.1006/gcen.1995.1126

subject

Has Abstract

pub_date

1995-10-01 00:00:00

pages

10-7

issue

1

eissn

0016-6480

issn

1095-6840

pii

S0016-6480(85)71126-4

journal_volume

100

pub_type

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