Abstract：
:Our previous study showed that docosahexaenoic acid (DHA) plays an important role in decreasing lipid accumulation by inducing apoptosis of the adipocytes in grass carp. However, the mechanism involved remains unclear. DHA has been reported as the natural ligand of PPARγ. The present study aimed to assess whether PPARγ mediates the pro-apoptotic effects by DHA. Adipocytes of grass carp were cultured until 2 days post-confluence and were treated with DHA at various concentrations-0, 25, 50, 100, 200, and 400 μmol/L for 24 h and at 200 μmol/L for various time periods (0, 12, 24, and 48 h, respectively). Besides, the adipocytes were exposed to 200 μM DHA and PPARγ antagonist or inhibitor of certain key enzymes of apoptosis, following which the expression levels of key genes of the cell apoptotic and mitochondrial apoptotic pathways were detected. We found that DHA induced apoptosis of grass carp adipocytes in a time- and dose-dependent manner (P < 0.05). In addition, DHA treatment significantly increased the protein and gene expression levels of PPARγ (P < 0.05), but the PPARγ antagonist significantly abolished this effect and the DHA pro-apoptotic effect (P < 0.05). Moreover, treatment with caspase 9 inhibitor significantly attenuated the DHA-induced preadipocytes apoptosis effects, while treatment with caspase 8 inhibitor showed no influence. These observations suggest that the DHA-induced apoptosis in adipocytes might be mediated by PPARγ and via the intrinsic apoptotic pathway in grass carp.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Jin A,Shi XC,Liu Y,Sun J,Ji Hdoi
10.1016/j.ygcen.2018.05.020subject
Has Abstractpub_date
2018-09-15 00:00:00pages
211-219eissn
0016-6480issn
1095-6840pii
S0016-6480(17)30789-Xjournal_volume
266pub_type
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1996.0073
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.12.017
更新日期：2009-04-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1994.1055
更新日期：1994-04-01 00:00:00
abstract：:Endocrinological research on wild animals inhabiting remote areas has been hampered by the need to store plasma samples at subzero temperatures. In an attempt to remedy this logistical issue, we here investigate the use of ethanol as an alternative to freezing for the preservation of steroid and indoleamine hormones i...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2006.09.014
更新日期：2007-01-15 00:00:00
